Sugar beet fiber(SBF)is a promising prebiotic candidate,yet its comprehensive impact on gut microbiota and metabolic profiles remains unclear.Here,we conducted in vitro fecal fermentation using samples from six health...Sugar beet fiber(SBF)is a promising prebiotic candidate,yet its comprehensive impact on gut microbiota and metabolic profiles remains unclear.Here,we conducted in vitro fecal fermentation using samples from six healthy donors to evaluate the microbial and metabolic impacts of SBF compared to a carbohydrate-free control(CON group).SBF significantly altered the overall gut microbiota composition and diversity(p<0.05).Keystone SBFresponsive taxa were identified,including the enrichment of Bifidobacterium,Parasutterella,Sutterella,Bifidobacterium longum subsp.longum,and Lactobacillus salivarius,alongside the reduction of Escherichia-Shigella.Network analysis revealed a functional guild of primary degraders actively foraging SBF.Metabolically,SBF supplementation significantly increased acetate,propionate,and butyrate production(p<0.05),while selectively modulating organic acids by elevating pyruvate and reducing 3-phosphoglycerate and citrate(p<0.05).Targeted metabolomics showed increased levels of indole-3-propionic acid(SBF:4.8797 mg/L vs.CON:4.4508 mg/L,p=0.0138)and tryptamine(SBF:8.2855 mg/L vs.CON:7.3250 mg/L,p=0.0030),along with a significant reduction in the secondary bile acid deoxycholic acid(p=0.0138).Non-targeted metabolomics further highlighted fatty acid biosynthesis as a key SBF-responsive pathway.These findings demonstrate that SBF promotes the growth of beneficial microbial taxa,enhances the production of short-chain fatty acids and indole derivatives,and remodels bile acid metabolism,supporting its potential as a novel prebiotic fiber for gut health improvement.展开更多
基金financial support from the National Natural Sci-ence Foundation of China(No.32202059)Natural Science Foundation of Jiangsu Province(BK20221070)+3 种基金the China National Postdoctoral Program for Innovative Talents(BX20230146)China Postdoctoral Science Foundation(2023M731338)the Fundamental Research Funds for the Central Universities(JUSRP123051)the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘Sugar beet fiber(SBF)is a promising prebiotic candidate,yet its comprehensive impact on gut microbiota and metabolic profiles remains unclear.Here,we conducted in vitro fecal fermentation using samples from six healthy donors to evaluate the microbial and metabolic impacts of SBF compared to a carbohydrate-free control(CON group).SBF significantly altered the overall gut microbiota composition and diversity(p<0.05).Keystone SBFresponsive taxa were identified,including the enrichment of Bifidobacterium,Parasutterella,Sutterella,Bifidobacterium longum subsp.longum,and Lactobacillus salivarius,alongside the reduction of Escherichia-Shigella.Network analysis revealed a functional guild of primary degraders actively foraging SBF.Metabolically,SBF supplementation significantly increased acetate,propionate,and butyrate production(p<0.05),while selectively modulating organic acids by elevating pyruvate and reducing 3-phosphoglycerate and citrate(p<0.05).Targeted metabolomics showed increased levels of indole-3-propionic acid(SBF:4.8797 mg/L vs.CON:4.4508 mg/L,p=0.0138)and tryptamine(SBF:8.2855 mg/L vs.CON:7.3250 mg/L,p=0.0030),along with a significant reduction in the secondary bile acid deoxycholic acid(p=0.0138).Non-targeted metabolomics further highlighted fatty acid biosynthesis as a key SBF-responsive pathway.These findings demonstrate that SBF promotes the growth of beneficial microbial taxa,enhances the production of short-chain fatty acids and indole derivatives,and remodels bile acid metabolism,supporting its potential as a novel prebiotic fiber for gut health improvement.
