Pollen tube growth is an essential step during flowering plant reproduction, whose growth depends on a population of dynamic apical actin filaments. Apical actin filaments were thought to be involved in the regu- lati...Pollen tube growth is an essential step during flowering plant reproduction, whose growth depends on a population of dynamic apical actin filaments. Apical actin filaments were thought to be involved in the regu- lation of vesicle fusion and targeting in the pollen tube. However, the molecular mechanisms that regulate the construction of apical actin structures in the pollen tube remain largely unclear. Here, we identify profilin as an important player in the regulation of actin polymerization at the apical membrane in the pollen tube. Downregulation of profilin decreased the amount of filamentous actin and induced disorganization of apical actin filaments, and reduced tip-directed vesicle transport and accumulation in the pollen tube. Direct visualization of actin dynamics revealed that the elongation of actin filaments originating at the apical membrane decreased in profilin mutant pollen tubes. Mutant profilin that is defective in binding poly-L-proline only partially rescues the actin polymerization defect in profilin mutant pollen tubes, although it fully rescues the actin turnover phenotype. We propose that profilin controls the construction of actin structures at the pollen tube tip, presumably by favoring formin-mediated actin polymerization at the apical membrane.展开更多
Thermally activated delayedfluorescence(TADF)molecules are regarded as promis-ing materials for realizing high-performance organic light-emitting diodes(OLEDs).The connecting groups between donor(D)and acceptor(A)units...Thermally activated delayedfluorescence(TADF)molecules are regarded as promis-ing materials for realizing high-performance organic light-emitting diodes(OLEDs).The connecting groups between donor(D)and acceptor(A)units in D–A type TADF molecules could affect the charge transfer and luminescence performance of TADF materials in aggregated states.In this work,we design and synthesize four TADF molecules using planar and twisted linkers to connect the aza-azulene donor(D)and triazine acceptor(A).Compared with planar linkers,the twisted ones(Az-NP-T and Az-NN-T)can enhance A–A aggregation interaction between adjacent molecules to balance hole and electron density.As a result,highly efficient and stable deep-red top-emission OLEDs with a high electroluminescence efficiency of 57.3%and an impressive long operational lifetime(LT_(95)∼30,000 h,initial luminance of 1000 cd m^(-2))are obtained.This study provides a new strategy for designing more effi-cient and stable electroluminescent devices through linker aggregation engineering in donor–acceptor molecules.展开更多
文摘Pollen tube growth is an essential step during flowering plant reproduction, whose growth depends on a population of dynamic apical actin filaments. Apical actin filaments were thought to be involved in the regu- lation of vesicle fusion and targeting in the pollen tube. However, the molecular mechanisms that regulate the construction of apical actin structures in the pollen tube remain largely unclear. Here, we identify profilin as an important player in the regulation of actin polymerization at the apical membrane in the pollen tube. Downregulation of profilin decreased the amount of filamentous actin and induced disorganization of apical actin filaments, and reduced tip-directed vesicle transport and accumulation in the pollen tube. Direct visualization of actin dynamics revealed that the elongation of actin filaments originating at the apical membrane decreased in profilin mutant pollen tubes. Mutant profilin that is defective in binding poly-L-proline only partially rescues the actin polymerization defect in profilin mutant pollen tubes, although it fully rescues the actin turnover phenotype. We propose that profilin controls the construction of actin structures at the pollen tube tip, presumably by favoring formin-mediated actin polymerization at the apical membrane.
基金National Key R&D Program of China,Grant/Award Number:2022YFE0109000National Natural Science Foundation of China,Grant/Award Number:21975152+1 种基金China Postdoctoral Science Foundation,Grant/Award Number:2022M722028Deutsche Forschungsgemeinschaft,Grant/Award Number:3DMM2O−EXC−2082/1−390761711。
文摘Thermally activated delayedfluorescence(TADF)molecules are regarded as promis-ing materials for realizing high-performance organic light-emitting diodes(OLEDs).The connecting groups between donor(D)and acceptor(A)units in D–A type TADF molecules could affect the charge transfer and luminescence performance of TADF materials in aggregated states.In this work,we design and synthesize four TADF molecules using planar and twisted linkers to connect the aza-azulene donor(D)and triazine acceptor(A).Compared with planar linkers,the twisted ones(Az-NP-T and Az-NN-T)can enhance A–A aggregation interaction between adjacent molecules to balance hole and electron density.As a result,highly efficient and stable deep-red top-emission OLEDs with a high electroluminescence efficiency of 57.3%and an impressive long operational lifetime(LT_(95)∼30,000 h,initial luminance of 1000 cd m^(-2))are obtained.This study provides a new strategy for designing more effi-cient and stable electroluminescent devices through linker aggregation engineering in donor–acceptor molecules.
