Stimuli-responsive drug delivery systems based on polymeric micelles can achieve controlled drug release to improve the therapeutic outcome and reduce unwanted systematic toxicity and side effects of the cytotoxic dru...Stimuli-responsive drug delivery systems based on polymeric micelles can achieve controlled drug release to improve the therapeutic outcome and reduce unwanted systematic toxicity and side effects of the cytotoxic drug in chemotherapy but often face challenging synthesis and purification of functionalized biocompatible polymer materials and low drug loading efficiency. In the present study, we reported a novel redox-responsive self-assembly polymeric micelle system, mPEG-β-CD/Ad-SS-CPT, to achieve high loading efficiency and selective delivery of camptothecin(CPT) in a reductive environment inside cancer cells. The host-vip supramolecular micelles utilized a simple β-CD modified PEG, mPEG-β-cyclodextrin(mPEG-β-CD), as the polymeric host with the ease of synthesis and purification. The vip prodrug Ad-SS-CPT contained the disulfide bond as the redox sensitivity group. The selective cleavage of disulfide bond and subsequent drug release in a reductive environment could potentially reduce system toxicity and improve the therapeutic outcome of CPT. In vitro studies showed that the micelles exhibited excellent cytotoxicity against He La cells comparable to the free drug. The host-vip polymeric micelles also showed great potentials for multi-drug co-delivery. Collectively, our current findings provided a general and convenient approach to design drug delivery systems based on stimuli-responsive polymeric micelles for disease treatment.展开更多
Fe_(2)O_(3)/Co_(3)O_(4)/NiO/NC nanosheets have been successfully prepared via a two-step annealing process of ternary metal coordination polymer. Attributing to the synergistic effects of the multiple metal oxides and...Fe_(2)O_(3)/Co_(3)O_(4)/NiO/NC nanosheets have been successfully prepared via a two-step annealing process of ternary metal coordination polymer. Attributing to the synergistic effects of the multiple metal oxides and the unique 2D nanosheet structure, the improved electrical conductivity and effective electron/ion transfer enables Fe_(2)O_(3)/Co_(3)O_(4)/NiO/NC electrode to exhibit excellent electrochemical properties with outstanding rate capacity and cycling stability. This work may pave the way to construct ternary metal oxide electrode material with an excellent electrochemical performance by introducing multiple metal oxides.展开更多
Dear Editor,Pain is a common and complex clinical problem which affects 28%-50%of the general population and is responsible for large health-related costs.People with chronic pain often develop nociceptive sensitizati...Dear Editor,Pain is a common and complex clinical problem which affects 28%-50%of the general population and is responsible for large health-related costs.People with chronic pain often develop nociceptive sensitization,having mood and anxiety disorders as comorbidities.Sustained pain may alter the processing of affective information in the brain,thereby inducing anxio-depressive disorders.展开更多
Optimizing lithium-ion batteries(LIBs)is pivotal for advancing sustainable energy solutions,particularly for portable electronics,electric vehicles and renewable energy storage systems.This review explores state-of-th...Optimizing lithium-ion batteries(LIBs)is pivotal for advancing sustainable energy solutions,particularly for portable electronics,electric vehicles and renewable energy storage systems.This review explores state-of-the-art strategies that integrate advanced digital simulations with comprehensive lifecycle management,assisted by artificial intelligence(AI),to overcome critical challenges in battery performance,safety,and durability.Our approach combines computational materials science with multi-scale modeling,bridging atomic-scale phenomena to system-level dynamics.This synergy provides new insights into materials behavior and electrochemical processes.Physics-based simulation techniques and AI-driven optimization technologies underpin these methods,enabling them to achieve accurate predictions and drive the design of nextgeneration batteries.Furthermore,the integration of cloud-based battery management systems(BMS)with edge computing facilitates real-time monitoring,predictive diagnostics,and proactive control,while the adoption of the“Battery Passport”concept enhances lifecycle traceability,promoting recycling and reuse.Collectively,these strategies establish a robust framework centered on standardization,modularization,and digitization,driving innovation across design,manufacturing,maintenance and recycling processes.This industry-academia-research collaborative battery large model has not only accelerated the industrialization of next-generation battery technologies but also provided strong support for the sustainable development of the sector.This review underscores the transformative potential of these integrated approaches,laying the groundwork for future breakthroughs in energy technologies and advancing global sustainability goals.展开更多
Dynamic temperature monitoring at critical locations of IGBT modules is a key means to improve the reliability of high-power converters.However,most ex-isting thermal model-based methods suffer from temper-ature estim...Dynamic temperature monitoring at critical locations of IGBT modules is a key means to improve the reliability of high-power converters.However,most ex-isting thermal model-based methods suffer from temper-ature estimation errors due to model parameter varia-tions and loss calculation errors.To address this problem,based on the reduced-order thermal model,an H_(∞)ob-server-based robust 3-D thermal monitoring method for IGBT modules is proposed in this paper.Through the optimized design of the observer feedback gain,the thermal model and real-time temperature information are effectively combined,which reduces the temperature estimation error in the worst case.Thus,the proposed method is more robust to model parameter uncertainty and loss error than the conventional temperature ob-servers.Experiment validations of the proposed H_(∞)ob-server and conventional observers are provided.The results demonstrate that the proposed observer achieves the highest temperature estimation accuracy under vari-ous system uncertainties,making it an effective solution for reliable online thermal monitoring of IGBT modules over the whole life cycle.展开更多
Burn wound healing is a multifaceted process often complicated by excessive inflammation and impaired ker-atinocyte function,both of which are key factors contributing to delayed healing.In this study we screened the ...Burn wound healing is a multifaceted process often complicated by excessive inflammation and impaired ker-atinocyte function,both of which are key factors contributing to delayed healing.In this study we screened the key miRNA regulating the epithelialization process under oxidative stress conditions through high-throughput sequencing.We identified that miR-192-5p was significantly upregulated in both oxidative stress models of keratinocytes and burn wound tissues,with detrimental effects on keratinocyte proliferation,migration,and apoptosis.Inhibition of miR-192-5p enhanced epidermal cell function by upregulating olfactomedin-4(OLFM4),a key gene associated with cell proliferation,adhesion and migration.To optimize delivery and therapeutic efficacy,we engineered MSC-derived exosomes loaded with antagomiR-192-5p(ant-192;Final content:2 nmol per wound;Loading efficiency:35.22±0.34%)and then encapsulated into a composite hydrogel composed of GelMA and MXene(Ti3C2Tx)nanosheets,forming a multifunctional dressing(Exo-ant-192@M-Gel).It achieved sustained release of ant-192,delay its degradation,and exert anti-inflammatory properties,thus promoting epithelization and burn wound healing.This study offered a novel therapeutic approach for burn wound closure.展开更多
Aging is an inevitable,physiological process of the human body,leading to deterioration in bodily function and increased susceptibility to various diseases.Effective endogenous therapeutic strategies for anti-aging an...Aging is an inevitable,physiological process of the human body,leading to deterioration in bodily function and increased susceptibility to various diseases.Effective endogenous therapeutic strategies for anti-aging and related diseases remain limited.Exercise confers multifaceted benefits to physical health by augmenting osteogenic and myogenic processes,enhancing cardiovascular and nervous system function,and attenuating chronic inflammation.Angiogenesis and lymphangiogenesis play pivotal roles in anti-aging,tissue repair,and immune response modulation,underscoring their potential as therapeutic targets for age-related diseases.Modulating angiogenic and lymphangiogenic pathways may provide a promising strategy for mitigating vascular decline and immune system dysfunction associated with aging.Exercise-induced endogenous angiogenesis and lymphangiogenesis can exert beneficial effects on physiological function,thereby representing a potential therapeutic paradigm for combating age-related decline and diseases.This review offers a thorough summary of the present knowledge regarding angiogenesis and lymphangiogenesis induced by exercise,encompassing the underlying mechanisms and the effects in different organs.In addition,it explores the potential of physical activity as a non-pharmacological intervention for anti-aging strategies and disease management,offering novel insights into the intersection of physical activity,aging,and disease progression.展开更多
Background:Sepsis-associated acute lung injury(ALl)is driven by endothelial barrier dysfunction and endothelial-mesenchymal transition(EndoMT),mediated by TGF-β1/SMAD3 signaling.Despite the therapeutic potential of S...Background:Sepsis-associated acute lung injury(ALl)is driven by endothelial barrier dysfunction and endothelial-mesenchymal transition(EndoMT),mediated by TGF-β1/SMAD3 signaling.Despite the therapeutic potential of SMAD3,current inhibitors face limitations.As endogenous small molecules that are closely related to physiological regulatory processes,microRNAs(miRNAs)have more potential research value for regulating SMAD3.Therefore,this study aimed to investigate the protective effect and molecular mechanism of a key miRNA targeting SMAD3 in sepsis-ALI.Methods:Screening multiple databases revealed that miR-23b-3p was the sole miRNA targeting SMAD3.Lipopolysaccharide(LPS)-stimulated human umbilical vein endothelial cells(HUvECs)and cecal ligation/puncture(CLP)mice were used to model sepsis.Lentivirus was used to construct stable strains.The functional performance and mechanism were verified by key techniques,including dual-luciferase assays,rescue experiments,reverse transcription-quantitative polymerase chain reaction(qPCR)/Western blotting,monocyte adhesion/permeability assays,and histopathology.Results:In LPS-stimulated HUVECs,miR-23b-3p downregulation correlated with TGF-β1/SMAD3 activation,EndoMT progression,and barrier disruption.miR-23b-3p overexpression reversed these effects by restoring the expression of junctional proteins and suppressing the expression of mesenchymal markers.Chromatin isolation by RNA purification-qPCR,RNA pull-down,and dual-luciferase assays confirmed the direct miR-23b-3p-SMAD33'UTR interaction.Rescue experiments demonstrated that miR-23b-3p counteracts TGF-β1/SMAD3 hyperactivation.In CLP mice,intratracheal agomiR-23b-3p attenuated lung injury,normalized alveolar architecture,and reduced vascular leakage by suppressing endothelial Smad3 upregulation.Conclusion:miR-23b-3p is a SMAD3-targeting regulator that inhibits EndoMT and repairs endothelial barrier integrity.Mechanistically,miR-23b-3p preserves endothelial homeostasis via SMAD3-dependent EndoMT inhibition.This study provides mechanistic insights and a miRNA-based therapeutic strategy for sepsis-induced ALl.展开更多
Background:Diabetic wounds are one of the most common and serious complications of diabetes mellitus,characterized by the dysfunction of wound-healing-related cells in quantity and quality.Our previous studies reveale...Background:Diabetic wounds are one of the most common and serious complications of diabetes mellitus,characterized by the dysfunction of wound-healing-related cells in quantity and quality.Our previous studies revealed that human amniotic epithelial cells(hAECs)could promote diabetic wound healing by paracrine action.Interestingly,numerous studies demonstrated that exosomes derived from stem cells are the critical paracrine vehicles for stem cell therapy.However,whether exosomes derived from hAECs(hAECs-Exos)mediate the effects of hAECs on diabetic wound healing remains unclear.This study aimed to investigate the biological effects of hAECs-Exos on diabetic wound healing and preliminarily elucidate the underlying mechanism.Methods:hAECs-Exos were isolated by ultracentrifugation and identified by transmission electron microscopy,dynamic light scattering and flow cytometry.A series of in vitro functional analyses were performed to assess the regulatory effects of hAECs-Exos on human fibroblasts(HFBs)and human umbilical vein endothelial cells(HUVECs)in a high-glycemic microenvironment.Highthroughput sequencing and bioinformatics analyses were conducted to speculate the related mechanisms of actions of hAECs-Exos on HFBs and HUVECs.Subsequently,the role of the candidate signaling pathway of hAECs-Exos in regulating the function of HUVECs and HFBs,as well as in diabetic wound healing,was assessed.Results:hAECs-Exos presented a cup-or sphere-shaped morphology with a mean diameter of 105.89±10.36 nm,were positive for CD63 and TSG101 and could be internalized by HFBs and HUVECs.After that,hAECs-Exos not only significantly promoted the proliferation and migration of HFBs,but also facilitated the angiogenic activity of HUVECs in vitro.High-throughput sequencing revealed enriched miRNAs of hAECs-Exos involved in wound healing.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses have shown that the target genes of the top 15 miRNAs were highly enriched in the PI3K-AKT pathway.Further functional studies demonstrated that the PI3K-AKT-mTOR pathway was necessary for the induced biological effects of hAECs-Exos on HFBs and HUVECs,as well as on wound healing,in diabetic mice.Conclusions:Our findings demonstrated that hAECs-Exos represent a promising,novel strategy for diabetic wound healing by promoting angiogenesis and fibroblast function via activation of the PI3K-AKT-mTOR pathway.展开更多
For the process of point cloud registration,and the problem of inaccurate registration due to errors in correspondence between keypoints.In this paper,a registration method based on calibration balls was proposed,the ...For the process of point cloud registration,and the problem of inaccurate registration due to errors in correspondence between keypoints.In this paper,a registration method based on calibration balls was proposed,the trunk,branch,and crown were selected as experimental objects,and three calibration balls were randomly placed around the experimental objects to ensure different distances between two ball centers.Using the Kinect V2 depth camera to collect the point cloud of the experimental scene from four different viewpoints,the PassThrough filter algorithm was used for point cloud filtering in each view of the experimental scenes.The Euclidean cluster extraction algorithm was employed for point cloud clustering and segmentation to extract the experimental object and the calibration ball.The random sample consensus(RANSAC)algorithm was applied to fit the point cloud of a ball and calculate the coordinates of the ball center so that the distance between two ball centers under different viewpoints can be obtained by using the coordinates of the ball center.Comparing the distance between the ball centers from different viewpoints to determine the corresponding relationship between the ball centers from different viewpoints,and then using the singular value decomposition(SVD)method,the initial registration matrix was obtained.Finally,Iterative Closest Point(ICP)and its improved algorithm were used for accurate registration.The experimental results showed that the method of point cloud registration based on calibration balls can solve the problem of corresponding error of keypoints,and can register point clouds from different viewpoints of the same object.The registration method was evaluated by using the registration running time and the fitness score.The final registration running time of different experimental objects was not more than 6.5 s.The minimum fitness score of the trunk was approximately 0.0001,the minimum fitness score of the branch was approximately 0.0001,and the minimum fitness score of the crown was approximately 0.0006.展开更多
The downwash flow field of the multi-rotor unmanned aerial vehicle(UAV), formed by propellers during operation, has a significant influence on the deposition, drift and distribution of droplets as well as the spray wi...The downwash flow field of the multi-rotor unmanned aerial vehicle(UAV), formed by propellers during operation, has a significant influence on the deposition, drift and distribution of droplets as well as the spray width of the UAV for plant protection. To study the general characteristics of the distribution of the downwash airflow and simulate the static wind field of multi-rotor UAVs in hovering state, a 3 D full-size physical model of JF01-10 six-rotor plant protection UAV was constructed using Solid Works. The entire flow field surrounding the UAV and the rotation flow fields around the six rotors were established in UG software. The physical model and flow fields were meshed using unstructured tetrahedral elements in ANSYS software.Finally, the downwash flow field of UAV was simulated.With an increased hovering height, the ground effect was reduced and the minimum current velocity increased initially and then decreased. In addition, the spatial proportion of the turbulence occupied decreased. Furthermore, the appropriate operational hovering height for the JF01-10 is considered to be 3 m. These results can be applied to six-rotor plant protection UAVs employed in pesticide spraying and spray width detection.展开更多
Diabetic wounds,characterized by prolonged inflammation and impaired vascularization,are a serious complication of diabetes.This study aimed to design a gelatin methacrylate(GelMA)hydrogel for the sustained release of...Diabetic wounds,characterized by prolonged inflammation and impaired vascularization,are a serious complication of diabetes.This study aimed to design a gelatin methacrylate(GelMA)hydrogel for the sustained release of netrin-1 and evaluate its potential as a scaffold to promote diabetic wound healing.The results showed that netrin-1 was highly expressed during the inflammation and proliferation phases of normal wounds,whereas it synchronously exhibited aberrantly low expression in diabetic wounds.Neutralization of netrin-1 inhibited normal wound healing,and the topical application of netrin-1 accelerated diabetic wound healing.Mechanistic studies demonstrated that netrin-1 regulated macrophage heterogeneity via the A2bR/STAT/PPARγsignaling pathway and promoted the function of endothelial cells,thus accelerating diabetic wound healing.These data suggest that netrin-1 is a potential therapeutic target for diabetic wounds.展开更多
Currently,Computational Fluid Dynamics(CFD)has been used to investigate agricultural UAV downwash.However,the validations of CFD models are difficult to deal with.Current verification methods are to use either water-s...Currently,Computational Fluid Dynamics(CFD)has been used to investigate agricultural UAV downwash.However,the validations of CFD models are difficult to deal with.Current verification methods are to use either water-sensitive papers or wind-speed arrays,which could get wind distribution or speed only.In this study,model migration was used to develop and verify downwash CFD models.The basic idea is to try to use the results of a scaled-down drone to represent that of a real-used UAV.The CFD models of both a real-used six-rotor UAV,JF01-10,and a 1:10 scaled-down small drone were developed by ANSYS.Then,the scaled-down drone was utilized to conduct trials by particle image velocimetry(PIV),so that not only distribution and speed but also flowing direction of downwash could be obtained.Results indicated the relative error between the PIV tests and the CFD models of the small UAV was less than 12%,while that between the tests and the CFD models of JF01-10 was less than 34%.It could be indicated that model migration could reflect multiple downwash characteristics but should be optimized in some complex details.This study was a preliminary but fundamental attempt to investigate CFD modelling and validation of agricultural UAVs and provided a novel thinking of downwash verification.展开更多
The vibration problem during the operation of rice transplanters is the most common phenomenon.In order that the static and dynamic characteristics of the rice transplanter chassis can meet the requirements of more st...The vibration problem during the operation of rice transplanters is the most common phenomenon.In order that the static and dynamic characteristics of the rice transplanter chassis can meet the requirements of more stable operation,the research took the 2ZG-6DK rice transplanter as the research object to carry out a vibration reduction optimization study.In the research,the Pro/Engineer 5.0 software was first used to model the chassis of the rice transplanter.The constructed finite element model was revised by using the structural parameter revision method and the mixed penalty function method.The model was imported into ANSYS Workbench to solve the modal frequency and vibration shape of the rice transplanter chassis.Based on the MAC(modal assurance criterion)criterion,modal tests were carried out to verify the accuracy of the finite element theoretical analysis.Through the analysis of the characteristics of the external excitation frequency,the chassis is structurally optimized to avoid resonance caused by the natural frequency of the chassis falling within the road excitation frequency range.The final optimization results showed that the first four orders of modal frequencies of the chassis were adjusted to 32.083 Hz,33.751 Hz,42.517 Hz,and 50.362 Hz,respectively,in the case that the chassis mass was increased by 6.714 kg(8.8%).They all avoid the range of road excitation frequency(10-30 Hz)so that the rice transplanter can effectively avoid the resonance phenomenon during operation.This study can provide a reference for the design and optimization of the chassis structure of transplanter.展开更多
基金National Key Research and Development Program of China (Grant No. 2019YFA0904800)Science and Technology Commission of Shanghai Municipality (Grant No. 11DZ2260600 and 10DZ2220500)Shanghai Natural Science Fund (Grant No. 20ZR1414700)。
文摘Stimuli-responsive drug delivery systems based on polymeric micelles can achieve controlled drug release to improve the therapeutic outcome and reduce unwanted systematic toxicity and side effects of the cytotoxic drug in chemotherapy but often face challenging synthesis and purification of functionalized biocompatible polymer materials and low drug loading efficiency. In the present study, we reported a novel redox-responsive self-assembly polymeric micelle system, mPEG-β-CD/Ad-SS-CPT, to achieve high loading efficiency and selective delivery of camptothecin(CPT) in a reductive environment inside cancer cells. The host-vip supramolecular micelles utilized a simple β-CD modified PEG, mPEG-β-cyclodextrin(mPEG-β-CD), as the polymeric host with the ease of synthesis and purification. The vip prodrug Ad-SS-CPT contained the disulfide bond as the redox sensitivity group. The selective cleavage of disulfide bond and subsequent drug release in a reductive environment could potentially reduce system toxicity and improve the therapeutic outcome of CPT. In vitro studies showed that the micelles exhibited excellent cytotoxicity against He La cells comparable to the free drug. The host-vip polymeric micelles also showed great potentials for multi-drug co-delivery. Collectively, our current findings provided a general and convenient approach to design drug delivery systems based on stimuli-responsive polymeric micelles for disease treatment.
基金supported by the Natural Science Foundation of Guangdong Province(No.2020A1515010886)the Science and Technology Planning Project of Guangzhou(No.202102010373)。
文摘Fe_(2)O_(3)/Co_(3)O_(4)/NiO/NC nanosheets have been successfully prepared via a two-step annealing process of ternary metal coordination polymer. Attributing to the synergistic effects of the multiple metal oxides and the unique 2D nanosheet structure, the improved electrical conductivity and effective electron/ion transfer enables Fe_(2)O_(3)/Co_(3)O_(4)/NiO/NC electrode to exhibit excellent electrochemical properties with outstanding rate capacity and cycling stability. This work may pave the way to construct ternary metal oxide electrode material with an excellent electrochemical performance by introducing multiple metal oxides.
基金supported by the National Natural Foundation of China(81571326 and 81501153)the Startup Funding of Shanghai Mental Health Center(2017-kyqd-01)+4 种基金Feixiang Personnel Training Program of Shanghai Mental Health Center(2018-FX-02)Shanghai Key Laboratory of Psychotic Disorders(13dz2260500)Natural Science Research Projects in Anhui Universities(KJ2020A0218)Applied Medicine Research Project of Hefei Health Committee(Hwk2020zd0016,AHWJ2021a036)Funding of Key Research and Development Projects of Anhui Province(2022e07020002).
文摘Dear Editor,Pain is a common and complex clinical problem which affects 28%-50%of the general population and is responsible for large health-related costs.People with chronic pain often develop nociceptive sensitization,having mood and anxiety disorders as comorbidities.Sustained pain may alter the processing of affective information in the brain,thereby inducing anxio-depressive disorders.
文摘Optimizing lithium-ion batteries(LIBs)is pivotal for advancing sustainable energy solutions,particularly for portable electronics,electric vehicles and renewable energy storage systems.This review explores state-of-the-art strategies that integrate advanced digital simulations with comprehensive lifecycle management,assisted by artificial intelligence(AI),to overcome critical challenges in battery performance,safety,and durability.Our approach combines computational materials science with multi-scale modeling,bridging atomic-scale phenomena to system-level dynamics.This synergy provides new insights into materials behavior and electrochemical processes.Physics-based simulation techniques and AI-driven optimization technologies underpin these methods,enabling them to achieve accurate predictions and drive the design of nextgeneration batteries.Furthermore,the integration of cloud-based battery management systems(BMS)with edge computing facilitates real-time monitoring,predictive diagnostics,and proactive control,while the adoption of the“Battery Passport”concept enhances lifecycle traceability,promoting recycling and reuse.Collectively,these strategies establish a robust framework centered on standardization,modularization,and digitization,driving innovation across design,manufacturing,maintenance and recycling processes.This industry-academia-research collaborative battery large model has not only accelerated the industrialization of next-generation battery technologies but also provided strong support for the sustainable development of the sector.This review underscores the transformative potential of these integrated approaches,laying the groundwork for future breakthroughs in energy technologies and advancing global sustainability goals.
基金supported by the National Key Research and Development Program of China(No.2022YFE0138400)Zhejiang Provincial Key R&D Program Project(No.2023C01061).
文摘Dynamic temperature monitoring at critical locations of IGBT modules is a key means to improve the reliability of high-power converters.However,most ex-isting thermal model-based methods suffer from temper-ature estimation errors due to model parameter varia-tions and loss calculation errors.To address this problem,based on the reduced-order thermal model,an H_(∞)ob-server-based robust 3-D thermal monitoring method for IGBT modules is proposed in this paper.Through the optimized design of the observer feedback gain,the thermal model and real-time temperature information are effectively combined,which reduces the temperature estimation error in the worst case.Thus,the proposed method is more robust to model parameter uncertainty and loss error than the conventional temperature ob-servers.Experiment validations of the proposed H_(∞)ob-server and conventional observers are provided.The results demonstrate that the proposed observer achieves the highest temperature estimation accuracy under vari-ous system uncertainties,making it an effective solution for reliable online thermal monitoring of IGBT modules over the whole life cycle.
基金supported by the National Key R&D Program of China(2024YFA1108401)the National Nature Science Foundation of China(81930057)+7 种基金Shanghai Rising Star Program(22QA1411700,24YF2758700)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-076)the National Nature Science Foundation of China(82372512,82372513,82072170 and 81772076)Shanghai Top Pri-ority Research Center Project(2023ZZ02013)Basic medical research project of Changhai Hospital(2023YQ02)Changhong talent plan of Changhai HospitalDeep Blue Talent Project of Naval Medical Univer-sityClinical Medical Research of Changhai Hospital(2024LYC06).
文摘Burn wound healing is a multifaceted process often complicated by excessive inflammation and impaired ker-atinocyte function,both of which are key factors contributing to delayed healing.In this study we screened the key miRNA regulating the epithelialization process under oxidative stress conditions through high-throughput sequencing.We identified that miR-192-5p was significantly upregulated in both oxidative stress models of keratinocytes and burn wound tissues,with detrimental effects on keratinocyte proliferation,migration,and apoptosis.Inhibition of miR-192-5p enhanced epidermal cell function by upregulating olfactomedin-4(OLFM4),a key gene associated with cell proliferation,adhesion and migration.To optimize delivery and therapeutic efficacy,we engineered MSC-derived exosomes loaded with antagomiR-192-5p(ant-192;Final content:2 nmol per wound;Loading efficiency:35.22±0.34%)and then encapsulated into a composite hydrogel composed of GelMA and MXene(Ti3C2Tx)nanosheets,forming a multifunctional dressing(Exo-ant-192@M-Gel).It achieved sustained release of ant-192,delay its degradation,and exert anti-inflammatory properties,thus promoting epithelization and burn wound healing.This study offered a novel therapeutic approach for burn wound closure.
基金supported by the grants from the National Key Research and Development Program of China Grant(No.2022YFA1104500)National Natural Science Foundation of China(Nos.82020108002,and 82225005)+1 种基金the and Science and Technology Commission of Shanghai Municipality(Nos.23410750100,20DZ2255400,and 21XD1421300)the“Dawn”Program of Shanghai Education Commission(No.19SG34).
文摘Aging is an inevitable,physiological process of the human body,leading to deterioration in bodily function and increased susceptibility to various diseases.Effective endogenous therapeutic strategies for anti-aging and related diseases remain limited.Exercise confers multifaceted benefits to physical health by augmenting osteogenic and myogenic processes,enhancing cardiovascular and nervous system function,and attenuating chronic inflammation.Angiogenesis and lymphangiogenesis play pivotal roles in anti-aging,tissue repair,and immune response modulation,underscoring their potential as therapeutic targets for age-related diseases.Modulating angiogenic and lymphangiogenic pathways may provide a promising strategy for mitigating vascular decline and immune system dysfunction associated with aging.Exercise-induced endogenous angiogenesis and lymphangiogenesis can exert beneficial effects on physiological function,thereby representing a potential therapeutic paradigm for combating age-related decline and diseases.This review offers a thorough summary of the present knowledge regarding angiogenesis and lymphangiogenesis induced by exercise,encompassing the underlying mechanisms and the effects in different organs.In addition,it explores the potential of physical activity as a non-pharmacological intervention for anti-aging strategies and disease management,offering novel insights into the intersection of physical activity,aging,and disease progression.
基金funded by the National Natural Science Foundation of China(81930057)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-076)+2 种基金Key Research Center Construction Project of Shanghai(2023ZZ02013)Major/Key Logistics Research Projects(AHJ24J005,JKBWS23C1018)Lab of Treatment of War Wounds During Amphibious Operations,and Clinical Key Discipline Project of Shanghai,PLA and PRC.
文摘Background:Sepsis-associated acute lung injury(ALl)is driven by endothelial barrier dysfunction and endothelial-mesenchymal transition(EndoMT),mediated by TGF-β1/SMAD3 signaling.Despite the therapeutic potential of SMAD3,current inhibitors face limitations.As endogenous small molecules that are closely related to physiological regulatory processes,microRNAs(miRNAs)have more potential research value for regulating SMAD3.Therefore,this study aimed to investigate the protective effect and molecular mechanism of a key miRNA targeting SMAD3 in sepsis-ALI.Methods:Screening multiple databases revealed that miR-23b-3p was the sole miRNA targeting SMAD3.Lipopolysaccharide(LPS)-stimulated human umbilical vein endothelial cells(HUvECs)and cecal ligation/puncture(CLP)mice were used to model sepsis.Lentivirus was used to construct stable strains.The functional performance and mechanism were verified by key techniques,including dual-luciferase assays,rescue experiments,reverse transcription-quantitative polymerase chain reaction(qPCR)/Western blotting,monocyte adhesion/permeability assays,and histopathology.Results:In LPS-stimulated HUVECs,miR-23b-3p downregulation correlated with TGF-β1/SMAD3 activation,EndoMT progression,and barrier disruption.miR-23b-3p overexpression reversed these effects by restoring the expression of junctional proteins and suppressing the expression of mesenchymal markers.Chromatin isolation by RNA purification-qPCR,RNA pull-down,and dual-luciferase assays confirmed the direct miR-23b-3p-SMAD33'UTR interaction.Rescue experiments demonstrated that miR-23b-3p counteracts TGF-β1/SMAD3 hyperactivation.In CLP mice,intratracheal agomiR-23b-3p attenuated lung injury,normalized alveolar architecture,and reduced vascular leakage by suppressing endothelial Smad3 upregulation.Conclusion:miR-23b-3p is a SMAD3-targeting regulator that inhibits EndoMT and repairs endothelial barrier integrity.Mechanistically,miR-23b-3p preserves endothelial homeostasis via SMAD3-dependent EndoMT inhibition.This study provides mechanistic insights and a miRNA-based therapeutic strategy for sepsis-induced ALl.
基金funded by the National Key R&D Program of China(2019YFA0110503)the National Nature Science Foundation of China(81701905,81930057,81772076,81871559,81571897)+5 种基金the Shanghai Pujiang Program(17PJD043)the Clinical Key Discipline Project of Shanghai and Chinathe Shanghai Health System Excellent Talent Training Program(2017BR037)the Fujian Burn Medical Center([2017]171)the Key Clinical Specialty Discipline Construction Programme of Fujian,China([2012]149)the Fujian Provincial Key Laboratory of Burn and Trauma,China.
文摘Background:Diabetic wounds are one of the most common and serious complications of diabetes mellitus,characterized by the dysfunction of wound-healing-related cells in quantity and quality.Our previous studies revealed that human amniotic epithelial cells(hAECs)could promote diabetic wound healing by paracrine action.Interestingly,numerous studies demonstrated that exosomes derived from stem cells are the critical paracrine vehicles for stem cell therapy.However,whether exosomes derived from hAECs(hAECs-Exos)mediate the effects of hAECs on diabetic wound healing remains unclear.This study aimed to investigate the biological effects of hAECs-Exos on diabetic wound healing and preliminarily elucidate the underlying mechanism.Methods:hAECs-Exos were isolated by ultracentrifugation and identified by transmission electron microscopy,dynamic light scattering and flow cytometry.A series of in vitro functional analyses were performed to assess the regulatory effects of hAECs-Exos on human fibroblasts(HFBs)and human umbilical vein endothelial cells(HUVECs)in a high-glycemic microenvironment.Highthroughput sequencing and bioinformatics analyses were conducted to speculate the related mechanisms of actions of hAECs-Exos on HFBs and HUVECs.Subsequently,the role of the candidate signaling pathway of hAECs-Exos in regulating the function of HUVECs and HFBs,as well as in diabetic wound healing,was assessed.Results:hAECs-Exos presented a cup-or sphere-shaped morphology with a mean diameter of 105.89±10.36 nm,were positive for CD63 and TSG101 and could be internalized by HFBs and HUVECs.After that,hAECs-Exos not only significantly promoted the proliferation and migration of HFBs,but also facilitated the angiogenic activity of HUVECs in vitro.High-throughput sequencing revealed enriched miRNAs of hAECs-Exos involved in wound healing.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses have shown that the target genes of the top 15 miRNAs were highly enriched in the PI3K-AKT pathway.Further functional studies demonstrated that the PI3K-AKT-mTOR pathway was necessary for the induced biological effects of hAECs-Exos on HFBs and HUVECs,as well as on wound healing,in diabetic mice.Conclusions:Our findings demonstrated that hAECs-Exos represent a promising,novel strategy for diabetic wound healing by promoting angiogenesis and fibroblast function via activation of the PI3K-AKT-mTOR pathway.
基金This research was funded by the National Key R&D Program of China(Grant No.2018YFD0700601)and the National Natural Science Foundation of China(Grant No.31600588).
文摘For the process of point cloud registration,and the problem of inaccurate registration due to errors in correspondence between keypoints.In this paper,a registration method based on calibration balls was proposed,the trunk,branch,and crown were selected as experimental objects,and three calibration balls were randomly placed around the experimental objects to ensure different distances between two ball centers.Using the Kinect V2 depth camera to collect the point cloud of the experimental scene from four different viewpoints,the PassThrough filter algorithm was used for point cloud filtering in each view of the experimental scenes.The Euclidean cluster extraction algorithm was employed for point cloud clustering and segmentation to extract the experimental object and the calibration ball.The random sample consensus(RANSAC)algorithm was applied to fit the point cloud of a ball and calculate the coordinates of the ball center so that the distance between two ball centers under different viewpoints can be obtained by using the coordinates of the ball center.Comparing the distance between the ball centers from different viewpoints to determine the corresponding relationship between the ball centers from different viewpoints,and then using the singular value decomposition(SVD)method,the initial registration matrix was obtained.Finally,Iterative Closest Point(ICP)and its improved algorithm were used for accurate registration.The experimental results showed that the method of point cloud registration based on calibration balls can solve the problem of corresponding error of keypoints,and can register point clouds from different viewpoints of the same object.The registration method was evaluated by using the registration running time and the fitness score.The final registration running time of different experimental objects was not more than 6.5 s.The minimum fitness score of the trunk was approximately 0.0001,the minimum fitness score of the branch was approximately 0.0001,and the minimum fitness score of the crown was approximately 0.0006.
基金supported and funded by The National Key Research and Development Program of China (2016YFD02 00700) from China Ministry of Science and TechnologyThe Fundamental Research Funds for the Central Universities (2015TC036 and 2017QC139)
文摘The downwash flow field of the multi-rotor unmanned aerial vehicle(UAV), formed by propellers during operation, has a significant influence on the deposition, drift and distribution of droplets as well as the spray width of the UAV for plant protection. To study the general characteristics of the distribution of the downwash airflow and simulate the static wind field of multi-rotor UAVs in hovering state, a 3 D full-size physical model of JF01-10 six-rotor plant protection UAV was constructed using Solid Works. The entire flow field surrounding the UAV and the rotation flow fields around the six rotors were established in UG software. The physical model and flow fields were meshed using unstructured tetrahedral elements in ANSYS software.Finally, the downwash flow field of UAV was simulated.With an increased hovering height, the ground effect was reduced and the minimum current velocity increased initially and then decreased. In addition, the spatial proportion of the turbulence occupied decreased. Furthermore, the appropriate operational hovering height for the JF01-10 is considered to be 3 m. These results can be applied to six-rotor plant protection UAVs employed in pesticide spraying and spray width detection.
基金supported by 173 plan project of Military Science and Technology(2019-JCJQ-ZD-359-00)the National Key R&D Program of China(2019YFA0110503,2019YFA0110501)+5 种基金the National Nature Science Foundation of China(82072170,82372512,82172201,82372513,81930057 and 81701905)Shanghai Rising Star Program(22QA1411700)Basic medical research project of Changhai Hospital(2023YQ02)Changhong talent plan of Changhai HospitalYouth Medical Talents-Specialist ProgramChinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2019-I2M-5-076).
文摘Diabetic wounds,characterized by prolonged inflammation and impaired vascularization,are a serious complication of diabetes.This study aimed to design a gelatin methacrylate(GelMA)hydrogel for the sustained release of netrin-1 and evaluate its potential as a scaffold to promote diabetic wound healing.The results showed that netrin-1 was highly expressed during the inflammation and proliferation phases of normal wounds,whereas it synchronously exhibited aberrantly low expression in diabetic wounds.Neutralization of netrin-1 inhibited normal wound healing,and the topical application of netrin-1 accelerated diabetic wound healing.Mechanistic studies demonstrated that netrin-1 regulated macrophage heterogeneity via the A2bR/STAT/PPARγsignaling pathway and promoted the function of endothelial cells,thus accelerating diabetic wound healing.These data suggest that netrin-1 is a potential therapeutic target for diabetic wounds.
基金The authors acknowledge that this work was financially supported by the National Key Research and Development Program of China(2018YFD0700603 and 2016YFD0200702)National Natural Science Foundation of China(31701315)+1 种基金Independent Innovation Foundation for the Graduates of China Agricultural University(2020XYZC38A)Chinese Universities Scientific Fund(2020TC116).The devices used in the paper were supported by National Research Center of Intelligent Equipment for Agriculture.
文摘Currently,Computational Fluid Dynamics(CFD)has been used to investigate agricultural UAV downwash.However,the validations of CFD models are difficult to deal with.Current verification methods are to use either water-sensitive papers or wind-speed arrays,which could get wind distribution or speed only.In this study,model migration was used to develop and verify downwash CFD models.The basic idea is to try to use the results of a scaled-down drone to represent that of a real-used UAV.The CFD models of both a real-used six-rotor UAV,JF01-10,and a 1:10 scaled-down small drone were developed by ANSYS.Then,the scaled-down drone was utilized to conduct trials by particle image velocimetry(PIV),so that not only distribution and speed but also flowing direction of downwash could be obtained.Results indicated the relative error between the PIV tests and the CFD models of the small UAV was less than 12%,while that between the tests and the CFD models of JF01-10 was less than 34%.It could be indicated that model migration could reflect multiple downwash characteristics but should be optimized in some complex details.This study was a preliminary but fundamental attempt to investigate CFD modelling and validation of agricultural UAVs and provided a novel thinking of downwash verification.
基金financially supported by the National Key Research and Development Program of China Subproject(Grant No.2021YFD2000601)Innovation Scientists and Technicians Talent Projects of Henan Provincial Department of Education(Grant No.23IRTSTHN015,No.202300410124)。
文摘The vibration problem during the operation of rice transplanters is the most common phenomenon.In order that the static and dynamic characteristics of the rice transplanter chassis can meet the requirements of more stable operation,the research took the 2ZG-6DK rice transplanter as the research object to carry out a vibration reduction optimization study.In the research,the Pro/Engineer 5.0 software was first used to model the chassis of the rice transplanter.The constructed finite element model was revised by using the structural parameter revision method and the mixed penalty function method.The model was imported into ANSYS Workbench to solve the modal frequency and vibration shape of the rice transplanter chassis.Based on the MAC(modal assurance criterion)criterion,modal tests were carried out to verify the accuracy of the finite element theoretical analysis.Through the analysis of the characteristics of the external excitation frequency,the chassis is structurally optimized to avoid resonance caused by the natural frequency of the chassis falling within the road excitation frequency range.The final optimization results showed that the first four orders of modal frequencies of the chassis were adjusted to 32.083 Hz,33.751 Hz,42.517 Hz,and 50.362 Hz,respectively,in the case that the chassis mass was increased by 6.714 kg(8.8%).They all avoid the range of road excitation frequency(10-30 Hz)so that the rice transplanter can effectively avoid the resonance phenomenon during operation.This study can provide a reference for the design and optimization of the chassis structure of transplanter.
