Bacillus thuringiensis（B.thuringiensis） is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins（Cry） are commonly used as biological alternatives to pesticides.In a pangenomic study,we sequenced ...Bacillus thuringiensis（B.thuringiensis） is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins（Cry） are commonly used as biological alternatives to pesticides.In a pangenomic study,we sequenced seven B.thuringiensis isolates in both high coverage and base-quality using the next-generation sequencing platform.The B.thuringiensis pangenome was extrapolated to have 4196 core genes and an asymptotic value of 558 unique genes when a new genome is added.Compared to the pangenomes of its closely related species of the same genus,B.thuringiensis pangenome shows an open characteristic,similar to B.cereus but not to B.anthracis;the latter has a closed pangenome. We also found extensive divergence among the seven B.thuringiensis genome assemblies,which harbor ample repeats and single nucleotide polymorphisms（SNPs）.The identities among orthologous genes are greater than 84.5%and the hotspots for the genome variations were discovered in genomic regions of 2.3-2.8 Mb and 5.0-5.6 Mb.We concluded that high-coverage sequence assemblies from multiple strains, before all the gaps are closed,are very useful for pangenomic studies.展开更多
Carotid atherosclerosis is a partial manifestation of arteriosclerosis of organism.In recent years,its incidence rate is increasing year by year.Traditional Chinese medicine(TCM)has a broad prospect in the clinical de...Carotid atherosclerosis is a partial manifestation of arteriosclerosis of organism.In recent years,its incidence rate is increasing year by year.Traditional Chinese medicine(TCM)has a broad prospect in the clinical development of CAS treatment by holism concept and the principle of treatment based on syndrome differentiation.This paper reviews the etiology,pathogenesis,TCM dialectics and TCM treatment of carotid atherosclerosis,in order to provide theoretical guidance for TCM treatment of CAS patients.展开更多
We aimed to firstly explore characteristics and prognostic factors of therapy-related acute myeloid leukemia(t-AML)in multi-center samples in China.We analyzed 228 t-AML patients from 14 centers across China.The media...We aimed to firstly explore characteristics and prognostic factors of therapy-related acute myeloid leukemia(t-AML)in multi-center samples in China.We analyzed 228 t-AML patients from 14 centers across China.The median age at t-AML diagnosis was 52 years(range,1.3–89 years).The median latency interval was 45.8 months(19.2–142.8 months).Next generation sequencing(NGS)results were available in 150(65.8%)patients.The overall survival(OS)and disease-free survival rates of all t-AML were 58.3%and 63.7%,respectively.In t-AML patients,NPM1 mutation(85.0%vs.54.4%,P=0.01),core binding factor(CBF)(RUNX1/RUNX1T1 and CBFβ/MYH11)(70.7%vs.55.1%,P=0.03),and allogeneic hematopoietic stem cell transplantation(allo-HSCT)(86.0%vs.67.9%,P=0.02)were associated with significantly better OS,while the 2022 ELN intermediate-adverse risk group had worse OS than the favorable group(55.9%vs.73.1%,P=0.02).In multivariable analyses,NPM1 mutation,CBF t-AML,primary tumor remission,WBC count≤15×10^(9)L^(-1),CRc after one course and allo-HSCT were associated with favorable OS.Regarding NGS molecular analysis,in addition to the positive effects of NPM1 on OS,our study identified TP53 mutation as a risk factor associated with poor OS(40.0%vs.66.6%,P=0.03,HR=2.64).We developed a prognostic scoring system including clinical and molecular profiles termed NTCTH(NPM1(HR=0.16),TP53(HR=3.45),CBF t-AML(HR=0.09),first course intensive induction Therapy regime(HR=0.24),and allo-HSCT(HR=0.36))in patients who performed NGS.Our study first demonstrated prognostic factors of t-AML in large samples from multiple centers in China and found that NPM1 and CBF t-AML were associated with superior OS,and TP53 was associated with inferior OS.展开更多
基金supported by a grant from King Abdulaziz City for Science and Technology,Riyadh,Saudi Arabia(No. KACST 428-29)institutional grant from CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics, Chinese Academy of Sciences+2 种基金supported by the grants from the National Basic Research Program(973 Program)(No.2010CB126604)the Special Foundation Work Program(No.2009FY 120100)the Ministry of Science and Technology of the People's Republic of China and from the National Science Foundation of China(No. 31071163).
文摘Bacillus thuringiensis（B.thuringiensis） is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins（Cry） are commonly used as biological alternatives to pesticides.In a pangenomic study,we sequenced seven B.thuringiensis isolates in both high coverage and base-quality using the next-generation sequencing platform.The B.thuringiensis pangenome was extrapolated to have 4196 core genes and an asymptotic value of 558 unique genes when a new genome is added.Compared to the pangenomes of its closely related species of the same genus,B.thuringiensis pangenome shows an open characteristic,similar to B.cereus but not to B.anthracis;the latter has a closed pangenome. We also found extensive divergence among the seven B.thuringiensis genome assemblies,which harbor ample repeats and single nucleotide polymorphisms（SNPs）.The identities among orthologous genes are greater than 84.5%and the hotspots for the genome variations were discovered in genomic regions of 2.3-2.8 Mb and 5.0-5.6 Mb.We concluded that high-coverage sequence assemblies from multiple strains, before all the gaps are closed,are very useful for pangenomic studies.
文摘Carotid atherosclerosis is a partial manifestation of arteriosclerosis of organism.In recent years,its incidence rate is increasing year by year.Traditional Chinese medicine(TCM)has a broad prospect in the clinical development of CAS treatment by holism concept and the principle of treatment based on syndrome differentiation.This paper reviews the etiology,pathogenesis,TCM dialectics and TCM treatment of carotid atherosclerosis,in order to provide theoretical guidance for TCM treatment of CAS patients.
基金supported by the National Key Research and Development Program of China(2022YFA1103300)Major Program of the National Natural Science Foundation of China(82293630)+2 种基金Beijing Municipal Science&Technology Commission(Z211100002921071)Peking University Medicine Fund for world's leading discipline or discipline cluster development(71003Y3035)Beijing Research Ward Excellence Program,BRWEP(BRWEP2024W134080106)。
文摘We aimed to firstly explore characteristics and prognostic factors of therapy-related acute myeloid leukemia(t-AML)in multi-center samples in China.We analyzed 228 t-AML patients from 14 centers across China.The median age at t-AML diagnosis was 52 years(range,1.3–89 years).The median latency interval was 45.8 months(19.2–142.8 months).Next generation sequencing(NGS)results were available in 150(65.8%)patients.The overall survival(OS)and disease-free survival rates of all t-AML were 58.3%and 63.7%,respectively.In t-AML patients,NPM1 mutation(85.0%vs.54.4%,P=0.01),core binding factor(CBF)(RUNX1/RUNX1T1 and CBFβ/MYH11)(70.7%vs.55.1%,P=0.03),and allogeneic hematopoietic stem cell transplantation(allo-HSCT)(86.0%vs.67.9%,P=0.02)were associated with significantly better OS,while the 2022 ELN intermediate-adverse risk group had worse OS than the favorable group(55.9%vs.73.1%,P=0.02).In multivariable analyses,NPM1 mutation,CBF t-AML,primary tumor remission,WBC count≤15×10^(9)L^(-1),CRc after one course and allo-HSCT were associated with favorable OS.Regarding NGS molecular analysis,in addition to the positive effects of NPM1 on OS,our study identified TP53 mutation as a risk factor associated with poor OS(40.0%vs.66.6%,P=0.03,HR=2.64).We developed a prognostic scoring system including clinical and molecular profiles termed NTCTH(NPM1(HR=0.16),TP53(HR=3.45),CBF t-AML(HR=0.09),first course intensive induction Therapy regime(HR=0.24),and allo-HSCT(HR=0.36))in patients who performed NGS.Our study first demonstrated prognostic factors of t-AML in large samples from multiple centers in China and found that NPM1 and CBF t-AML were associated with superior OS,and TP53 was associated with inferior OS.
