[Objectives]To investigate the synergistic effect of Arnebiae Radix after processing.[Methods]The effects of raw Arnebiae Radix and milk processed Arnebiae Radix on hypothermia in yeast-induced febrile rats were compa...[Objectives]To investigate the synergistic effect of Arnebiae Radix after processing.[Methods]The effects of raw Arnebiae Radix and milk processed Arnebiae Radix on hypothermia in yeast-induced febrile rats were compared.[Results]The processed and unprocessed Arnebiae Radix at high,medium and low doses all had a certain effect on inhibiting the rise of body temperature in rats.The high dose unprocessed group,the medium dose processed group and the high dose processed group had the best inhibitory effect on body temperature,the low dose processed group could delay the fever time,and the low dose unprocessed group had poor inhibitory effect on fever.[Conclusions]The prepared Arnebiae Radix has enhanced drug effect,and milk processed Arnebiae Radix can be used to replace common Arnebiae Radix to reduce the dosage of Arnebiae Radix and save Arnebiae Radix resources.展开更多
Clear cell renal cell carcinoma(ccRCC)is the most prevalent and aggressive subtype of kidney cancer,demanding rapid and precise diagnostic tools to guide clinical decision-making.Current methods,such as immunohistoche...Clear cell renal cell carcinoma(ccRCC)is the most prevalent and aggressive subtype of kidney cancer,demanding rapid and precise diagnostic tools to guide clinical decision-making.Current methods,such as immunohistochemistry(IHC)and frozen section analysis,face limitations in speed,sensitivity,and workflow complexity.To address these challenges,we developed a tetrahedral DNA nanostructure(TDN)-enhanced quadrivalent hybridization chain reaction(qHCR)platform targeting carbonic anhydrase IX(CAIX),a highly specific biomarker for ccRCC.This system integrates aptamer-based molecular recognition with enzyme-free signal amplification,leveraging the spatial confinement and multivalent effects of TDNs to achieve ultrasensitive detection.The qHCR platform demonstrated remarkable performance,with a reaction rate 95 times faster than traditional HCR and sustained signal stability in serum for over 36 h.In clinical validation using 40 ccRCC tissue samples,the platform enabled Precision Tumor Recognition within 25 min for frozen sections,yielding a tumor-to-normal tissue signal ratio of 3.35:1,and completed molecular profiling within 2.5 h for formalin-fixed,paraffin-embedded(FFPE)samples,showing full concordance with histopathological diagnoses.Its modular design allows seamless target switching by replacing aptamer sequences,as confirmed by successful detection of protein tyrosine kinase(PTK7)in an acute lymphoblastic leukemia model.With its cost-effectiveness($0.15 per test),streamlined workflow,and compatibility with both intraoperative margin assessment and postoperative pathological analysis,the qHCR platform represents a transformative advancement in molecular diagnostics for ccRCC management,offering a robust solution for precision oncology and time-critical surgical decision-making.展开更多
Exploiting cells as vehicles combined with nanoparticles combined with therapy has attracted increasing attention in the world recently. Red blood cells, leukocytes and stem cells have been used for tumor immunotherap...Exploiting cells as vehicles combined with nanoparticles combined with therapy has attracted increasing attention in the world recently. Red blood cells, leukocytes and stem cells have been used for tumor immunotherapy, tissue regeneration and inflammatory disorders, and it is known that neutrophils can accumulate in brain lesions in many brain diseases including depression. N-Acetyl Pro–Gly–Pro(PGP) peptide shows high specific binding affinity to neutrophils through the CXCR2 receptor. In this study, PGP was used to modify baicalein-loaded solid lipid nanoparticles(PGP-SLNs) to facilitate binding to neutrophils in vivo. Brain-targeted delivery to the basolateral amygdala(BLA) was demonstrated by enhanced concentration of baicalein in the BLA. An enhanced anti-depressant effect was observed in vitro and in vivo. The mechanism involved inhibition of apoptosis and a decrease in lactate dehydrogenase release. Behavioral evaluation carried out with rats demonstrated that anti-depression outcomes were achieved. The results indicate that PGP-SLNs decrease immobility time, increase swimming time and climbing time and attenuate locomotion in olfactory-bulbectomized(OB) rats. In conclusion, PGP modification is a strategy for targeting the brain with a cell–nanoparticle delivery system for depression therapy.展开更多
To the Editor:Acupoint sticking therapy reportedly enhances the therapeutic efficacy of drugs,[1]with corresponding acupoints for the treatment of diseases,such as rheumatoid arthritis(RA)and dysmenorrhea.However,repo...To the Editor:Acupoint sticking therapy reportedly enhances the therapeutic efficacy of drugs,[1]with corresponding acupoints for the treatment of diseases,such as rheumatoid arthritis(RA)and dysmenorrhea.However,reports on the differences in the concentration and efficiency of bioactive constituents for acupoint and nonacupoint applications are lacking.The pharmacokinetics/pharmacodynamics(PK/PD)model is suitable for explaining bioactive constituents and pharmaceutical effects in vivo,and the relationship between PK and PD has been applied in traditional Chinese medicine(TCM)studies.展开更多
基金Health Science and Technology Program of Inner Mongolia Autonomous Region(202201450)Key R&D and Achievement Transformation Project of Inner Mongolia Autonomous Region(2023YFDZ0063)+3 种基金Project of Improving the Scientific Research and Innovation Ability of Young Teachers in Universities Directly under Inner Mongolia Autonomous Region(GXKY22139)Open Fund Project of National and Local Joint Engineering Research Center for Mongolian Medicine Research and Development(MDK2021026)Key Discipline Construction Project of Traditional Chinese Pharmacy(Mongolian Pharmacy)of Inner Mongolia Minzu University(ZYX007)Basic Operating Expenses for Scientific Research of Universities Directly under Inner Mongolia Autonomous Region in 2022"Study on the Change of Shikonin Content During Milk Processed Arnebiae Radix".
文摘[Objectives]To investigate the synergistic effect of Arnebiae Radix after processing.[Methods]The effects of raw Arnebiae Radix and milk processed Arnebiae Radix on hypothermia in yeast-induced febrile rats were compared.[Results]The processed and unprocessed Arnebiae Radix at high,medium and low doses all had a certain effect on inhibiting the rise of body temperature in rats.The high dose unprocessed group,the medium dose processed group and the high dose processed group had the best inhibitory effect on body temperature,the low dose processed group could delay the fever time,and the low dose unprocessed group had poor inhibitory effect on fever.[Conclusions]The prepared Arnebiae Radix has enhanced drug effect,and milk processed Arnebiae Radix can be used to replace common Arnebiae Radix to reduce the dosage of Arnebiae Radix and save Arnebiae Radix resources.
基金supported by the National Natural Science Foundation of China(grant number 72174144)the Natural Science Foundation of Tianjin(grant number 25JCLZJC00250)the Tianjin Health Research Project(grant number TJWJ2025RC004)。
文摘Clear cell renal cell carcinoma(ccRCC)is the most prevalent and aggressive subtype of kidney cancer,demanding rapid and precise diagnostic tools to guide clinical decision-making.Current methods,such as immunohistochemistry(IHC)and frozen section analysis,face limitations in speed,sensitivity,and workflow complexity.To address these challenges,we developed a tetrahedral DNA nanostructure(TDN)-enhanced quadrivalent hybridization chain reaction(qHCR)platform targeting carbonic anhydrase IX(CAIX),a highly specific biomarker for ccRCC.This system integrates aptamer-based molecular recognition with enzyme-free signal amplification,leveraging the spatial confinement and multivalent effects of TDNs to achieve ultrasensitive detection.The qHCR platform demonstrated remarkable performance,with a reaction rate 95 times faster than traditional HCR and sustained signal stability in serum for over 36 h.In clinical validation using 40 ccRCC tissue samples,the platform enabled Precision Tumor Recognition within 25 min for frozen sections,yielding a tumor-to-normal tissue signal ratio of 3.35:1,and completed molecular profiling within 2.5 h for formalin-fixed,paraffin-embedded(FFPE)samples,showing full concordance with histopathological diagnoses.Its modular design allows seamless target switching by replacing aptamer sequences,as confirmed by successful detection of protein tyrosine kinase(PTK7)in an acute lymphoblastic leukemia model.With its cost-effectiveness($0.15 per test),streamlined workflow,and compatibility with both intraoperative margin assessment and postoperative pathological analysis,the qHCR platform represents a transformative advancement in molecular diagnostics for ccRCC management,offering a robust solution for precision oncology and time-critical surgical decision-making.
基金financial support from the National Natural Science Foundation of China (81673372, 81690263, 81361140344 and 81773911)the National Basic Research Program of China (2013CB 932502)+2 种基金the Opening Project of Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education (Sichuan University, Chengdu, China)the Development Project of Shanghai Peak Disciplines–Integrated Medicine (No. 20150407)the Open Project Program of Key Lab of Smart Drug Delivery (Fudan University, Shanghai, China), Ministry of Education, China
文摘Exploiting cells as vehicles combined with nanoparticles combined with therapy has attracted increasing attention in the world recently. Red blood cells, leukocytes and stem cells have been used for tumor immunotherapy, tissue regeneration and inflammatory disorders, and it is known that neutrophils can accumulate in brain lesions in many brain diseases including depression. N-Acetyl Pro–Gly–Pro(PGP) peptide shows high specific binding affinity to neutrophils through the CXCR2 receptor. In this study, PGP was used to modify baicalein-loaded solid lipid nanoparticles(PGP-SLNs) to facilitate binding to neutrophils in vivo. Brain-targeted delivery to the basolateral amygdala(BLA) was demonstrated by enhanced concentration of baicalein in the BLA. An enhanced anti-depressant effect was observed in vitro and in vivo. The mechanism involved inhibition of apoptosis and a decrease in lactate dehydrogenase release. Behavioral evaluation carried out with rats demonstrated that anti-depression outcomes were achieved. The results indicate that PGP-SLNs decrease immobility time, increase swimming time and climbing time and attenuate locomotion in olfactory-bulbectomized(OB) rats. In conclusion, PGP modification is a strategy for targeting the brain with a cell–nanoparticle delivery system for depression therapy.
基金National Natural Science Foundation of China(Nos. 82074271 and 81473359)Natural Science Foundation of Heilongjiang Province(No. H201472)Central Government Supports Local College Reform Projects(No. 2020YQ05)
文摘To the Editor:Acupoint sticking therapy reportedly enhances the therapeutic efficacy of drugs,[1]with corresponding acupoints for the treatment of diseases,such as rheumatoid arthritis(RA)and dysmenorrhea.However,reports on the differences in the concentration and efficiency of bioactive constituents for acupoint and nonacupoint applications are lacking.The pharmacokinetics/pharmacodynamics(PK/PD)model is suitable for explaining bioactive constituents and pharmaceutical effects in vivo,and the relationship between PK and PD has been applied in traditional Chinese medicine(TCM)studies.
