Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due t...Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.展开更多
Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accu...Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy.Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest.In this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named BnaPan50T.The platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic traits.The capture quality of BnaPan50T was demonstrated well in 12 typical accessions.Compared with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide application.Target capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the platform.BnaPan50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification resources.This study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.展开更多
Rapeseed(Brassica napus)is one of the most important oil crops worldwide;however,drought seriously curtails its growth and productivity.Identifying drought-tolerant germplasm is an efficient strategy for addressing wa...Rapeseed(Brassica napus)is one of the most important oil crops worldwide;however,drought seriously curtails its growth and productivity.Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages.Here,we phenotyped a panel of 264 B.napus accessions at full-bloom stage using water loss ratio(WLR)as drought-tolerant index.It identified 8 low-WLR and 6 high-WLR accessions,regarded as droughttolerant and drought-sensitive,respectively.Comparing with drought-sensitive accessions at the seedling stage,drought-tolerant accessions had shown better performance in maintaining fresh and dry weights,and performed the higher expression of drought-induced marker genes under drought stress.Subsequently,a total of 139 SNPs(single nucleotide polymorphisms)were identified associated with the WLR using a genome-wide association study(GWAS)among 264 B.napus accessions,with the largest number SNPs at chromosome A10,and 13 SNPs significantly were associated with the WLR(-log_(10)(p-value)>6).Furthermore,4 putative candidate genes(BnaC09.RPS6,BnaC09.MATE,BnaA10.PPD5 and BnaC09.Histone)were screened involving in drought tolerance in B.napus.Together,our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed.展开更多
Rapeseed(Brassica napus L.)is the second most widely grown premium oilseed crop globally,mainly for its vegetable oil and protein meal.One of the main goals of breeders is producing high-yield rapeseed cultivars with ...Rapeseed(Brassica napus L.)is the second most widely grown premium oilseed crop globally,mainly for its vegetable oil and protein meal.One of the main goals of breeders is producing high-yield rapeseed cultivars with sustainable production to meet the requirements of the fast-growing population.Besides the pod number,seeds per silique(SS),and thousand-seed weight(TSW),the ovule number(ON)is a decisive yield determining factor of individual plants and the final seed yield.In recent years,tremendous efforts have been made to dissect the genetic and molecular basis of these complex traits,but relatively few genes or loci controlling these traits have been reported thus far.This review highlights the updated information on the hormonal and molecular basis of ON and development in model plants(Arabidopsis thaliana).It also presents what is known about the hormonal,molecular,and genetic mechanism of ovule development and number,and bridges our understanding between the model plant species(A.thaliana)and cultivated species(B.napus).This report will open new pathways for primary and applied research in plant biology and benefit rapeseed breeding programs.This synopsis will stimulate research interest to further understand ovule number determination,its role in yield improvement,and its possible utilization in breeding programs.展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32272111)+4 种基金Special fund for youth team of the Southwest Universities(SWU-XJPY202306)Chongqing Natural Science Foundation(CSTB2024NSCQLZX0012)Modern Agro-industry Technology Research System(CARS-12)Chongqing Modern Agricultural Industry Technology System(COMAITS202504)Biological Breeding-National Science and Technology Major Project(2022ZD04008).We sincerely appreciate the Plant Editors team for English language editing of the manuscript,which significantly improved its clarity and overall quality.
文摘Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.
基金supported by the National Natural Science Foundation of China(31871653 to K.L.,31830067 to J.L.)the Talent Project of Chongqing Natural Science Foundation(cstc2021ycjhbgzxm0033 to K.L.)Germplasm Creation Special Program of Southwest University.
文摘Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy.Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest.In this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named BnaPan50T.The platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic traits.The capture quality of BnaPan50T was demonstrated well in 12 typical accessions.Compared with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide application.Target capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the platform.BnaPan50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification resources.This study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.
基金supported by grants from the National Key Research and Development Plan(2018YFD0100500)the National Natural Science Foundation of China(31871653)+3 种基金the Fundamental Research Funds for the Central Universities(XDJK2020D023)the Chongqing Postgraduate Research and Innovation Projects(CYS20124)the Natural Science Foundation of Chongqing,China(cstc2018jcyjAX0347)the 111 project(B12006).
文摘Rapeseed(Brassica napus)is one of the most important oil crops worldwide;however,drought seriously curtails its growth and productivity.Identifying drought-tolerant germplasm is an efficient strategy for addressing water shortages.Here,we phenotyped a panel of 264 B.napus accessions at full-bloom stage using water loss ratio(WLR)as drought-tolerant index.It identified 8 low-WLR and 6 high-WLR accessions,regarded as droughttolerant and drought-sensitive,respectively.Comparing with drought-sensitive accessions at the seedling stage,drought-tolerant accessions had shown better performance in maintaining fresh and dry weights,and performed the higher expression of drought-induced marker genes under drought stress.Subsequently,a total of 139 SNPs(single nucleotide polymorphisms)were identified associated with the WLR using a genome-wide association study(GWAS)among 264 B.napus accessions,with the largest number SNPs at chromosome A10,and 13 SNPs significantly were associated with the WLR(-log_(10)(p-value)>6).Furthermore,4 putative candidate genes(BnaC09.RPS6,BnaC09.MATE,BnaA10.PPD5 and BnaC09.Histone)were screened involving in drought tolerance in B.napus.Together,our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed.
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32272111)+4 种基金the Special fund for youth team of the Southwest Universities,China(SWU-XJPY202306)the Chongqing Natural Science Foundation,China(CSTB2024NSCQ-LZX0012)the Chongqing Modern Agricultural Industry Technology System,China(COMAITS202304)the Chongqing Germplasm Resource Bank,China(ZWZZ2020004)the Germplasm Creation Special Program of Southwest University,China。
文摘Rapeseed(Brassica napus L.)is the second most widely grown premium oilseed crop globally,mainly for its vegetable oil and protein meal.One of the main goals of breeders is producing high-yield rapeseed cultivars with sustainable production to meet the requirements of the fast-growing population.Besides the pod number,seeds per silique(SS),and thousand-seed weight(TSW),the ovule number(ON)is a decisive yield determining factor of individual plants and the final seed yield.In recent years,tremendous efforts have been made to dissect the genetic and molecular basis of these complex traits,but relatively few genes or loci controlling these traits have been reported thus far.This review highlights the updated information on the hormonal and molecular basis of ON and development in model plants(Arabidopsis thaliana).It also presents what is known about the hormonal,molecular,and genetic mechanism of ovule development and number,and bridges our understanding between the model plant species(A.thaliana)and cultivated species(B.napus).This report will open new pathways for primary and applied research in plant biology and benefit rapeseed breeding programs.This synopsis will stimulate research interest to further understand ovule number determination,its role in yield improvement,and its possible utilization in breeding programs.
