[Objectives] To study the differences in the effects of Rheum officinale and wine prepared Rheum palmatum Dachengqi Decoction(DCQ) on laxative effect of mice.[Methods]First,72 mice were randomly divided into positive ...[Objectives] To study the differences in the effects of Rheum officinale and wine prepared Rheum palmatum Dachengqi Decoction(DCQ) on laxative effect of mice.[Methods]First,72 mice were randomly divided into positive control group,blank control group,6 and10 g/kg dose R.officinale DCQ groups,6 and 10 g/kg dose wine prepared R.palmatum DCQ groups.After administration at the dose of20 m L/kg,metabolic cage method and carbon powder propulsion were used to conduct Na+-K+-ATPasc activity experiment,and the comparison was made for the differences in the normal laxative effect of R.officinale and wine prepared R.palmatum on DCQ,the carbon powder propulsion rate,and Na+-K+-ATPasc activit.[Results]There are differences in the laxative effect of R.officinale and wine prepared R.palmatum on DCQ,but their differences in changes are,to some extent,connected with R.officinale and wine prepared R.palmatum.From comparison of R.officinale DCQ group,wine prepared R.palmatum DCQ group,blank control group,and positive control group,it is found that there are differences in the normal laxative effect of normal mice,carbon powder propulsion,and inhibition of Na+-K+-ATPase activity.In addition,the comparison of drug administration groups indicates that the differences in the effects of R.officinale DCQ group are most significant,and there are significant differences in the 6 and 10 g/kg groups.[Conclusions]There are certain differences in the influence of R.officinale and wine prepared R.palmatum Dachengqi Decoction on normal laxative effect of mice,which may be connected with the fact that changes in main chemical components of king drugs R.officinale and wine prepared R.palmatum lead to changes in the dissolution amount of anthraquinones in the compound Dachengqi Decoction.展开更多
In both Traditional Chinese Medicine(TCM)and modern medicine,they agree that the integrity and healthy structure of the vascular endothelium are essential for normal hemodynamics.Damage to the vascular endothelium can...In both Traditional Chinese Medicine(TCM)and modern medicine,they agree that the integrity and healthy structure of the vascular endothelium are essential for normal hemodynamics.Damage to the vascular endothelium can quickly activate the extrinsic coagulation pathway by triggering the tissue factor(TF)and lead to coagulation.This damage,along with a loss of anticoagulant properties through antithrombinⅢ(ATⅢ),TF pathway inhibitors,and the protein C system,can result in a hypercoagulable state and even thrombosis.Hypercoagulability is not only a common feature of many cancers but also an important factor promoting tumor development and metastasis,which corresponds to the TCM theory of“blood stasis leading to tumors.”The pharmacological effects of heparin and aspirin have similarities with TCM's“activating blood circulation and removing blood stasis”theory in improving blood circulation,treating related diseases,and their anti-inflammatory effects.展开更多
Natural products(NPs)have historically been a fundamental source for drug discovery.Yet the complex nature of NPs presents substantial challenges in pinpointing bioactive constituents,and corresponding targets.In the ...Natural products(NPs)have historically been a fundamental source for drug discovery.Yet the complex nature of NPs presents substantial challenges in pinpointing bioactive constituents,and corresponding targets.In the present study,an innovative natural product virtual screening-interaction-phenotype(NP-VIP)strategy that integrates virtual screening,chemical proteomics,and metabolomics to identify and validate the bioactive targets of NPs.This approach reduces false positive results and enhances the efficiency of target identification.Salvia miltiorrhiza(SM),a herb with recognized therapeutic potential against ischemic stroke(IS),was used to illustrate the workflow.Utilizing virtual screening,chemical proteomics,and metabolomics,potential therapeutic targets for SM in the IS treatment were identified,totaling 29,100,and 78,respectively.Further analysis via the NP-VIP strategy highlighted five high-confidence targets,including poly[ADP-ribose]polymerase 1(PARP1),signal transducer and activator of transcription 3(STAT3),amyloid precursor protein(APP),glutamate-ammonia ligase(GLUL),and glutamate decarboxylase 67(GAD67).These targets were subsequently validated and found to play critical roles in the neuroprotective effects of SM.The study not only underscores the importance of SM in treating IS but also sets a precedent for NP research,proposing a comprehensive approach that could be adapted for broader pharmacological explorations.展开更多
Turmeric residue（TR）, containing residual levels of curcumin, is a solid by-product waste generated after the extraction and separation of curcumin from turmeric root. A feeding trial was conducted to evaluate the e...Turmeric residue（TR）, containing residual levels of curcumin, is a solid by-product waste generated after the extraction and separation of curcumin from turmeric root. A feeding trial was conducted to evaluate the effects of TR on the survival of Chinese soft-shelled turtles（SSTs）, Pelodiscus sinensis, under a high ambient temperature. A total of 320 female SSTs were assigned randomly to two diets： basal diet（the control group, n=160） and an interventional diet supplemented with 10% TR（the TR group, n=160）. Our results demonstrated that supplementation of TR increased the SST survival rate by 135.5%, and superoxide dismutase（SOD） activity of SST liver by 112.8%, and decreased the malondialdehyde（MDA） content of SST liver by 36.4%, compared to the control group. The skin of the SST fed TR showed a golden color. High-performance liquid chromatography（HPLC） analysis indicated that the concentrations of curcumin in TR and the skin of the SST fed TR were（1.69±0.30） and（0.14±0.03） μg/g, respectively. Our observation suggests that supplementation of TR increased the survival rate of SST under high ambient temperatures. We speculated that the increased survival rate and tolerance at the high ambient temperature were associated with the anti-oxidation activity of curcumin from TR. Moreover, curcumin in TR could be deposited in SST skin, which made it more favored in the market of China. Our findings provide new knowledge and evidence to effectively reuse TR as a feed additive in animal and aquatic farming.展开更多
Background The molecular mechanisms of early-onset multigenerational diabetes remain unknown.This study aimed to investigate the clinical and genetic characteristics of early-onset diabetes involving at least two cons...Background The molecular mechanisms of early-onset multigenerational diabetes remain unknown.This study aimed to investigate the clinical and genetic characteristics of early-onset diabetes involving at least two consecutive generations.Methods From 1296 inpatients with diabetes,we selected individuals who were≤30 years of age and who were clinically suspected of having familial monogenic diabetes.Clinical data were collected from the probands and their family members.Whole-exome sequencing(WES)was used to identify possible causal variants for diabetes.Candidate pathogenic variants were verified by Sanger sequencing,assessed for cosegregation in family members,and evaluated on the basis of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology(ACMG/AMP)guidelines.Moreover,missense and synonymous variants were subjected to in silico pathogenicity prediction via MutationTaster and PolyPhen-2.RNAfold was used to predict RNA structural alterations for synonymous variants.Results Twenty-five early-onset diabetes patients with a history of familial diabetes were enrolled.Pathogenic/likely pathogenic variants(p.Gly292fs in HNF1A,p.Gly245Argfs*22 in PDX1,p.Asp329His in KCNJ11,p.Leu734Phe and p.Val606Gly in WFS1)were detected in four patients,who were diagnosed accurately and treated with reasonable hypoglycemic agents based on genetic testing results.The variants of uncertain significance(ABCC8 c.3039 G>A(p.Ser1013=Ser),MAPK8IP1 p.Gln144_Gly145insSerGln,and TBC1D4 p.Arg1249Trp)were identified in three probands.Conclusion Patients with early-onset diabetes involving at least two consecutive generations may harbor genetic variants.Genetic testing in this population enables precision diagnosis,informs individualized treatment,and facilitates genetic counseling.展开更多
Herein,a ternary supramolecular assembly(BPP-BQ?CB[8]-SCD)is successfully constructed by a bromophenylpyridine-tethered-bromoisoquinoline(BPP-BQ),cucurbit[8]uril(CB[8])and sulfonatedβ-cyclodextrin(SCD)via successive ...Herein,a ternary supramolecular assembly(BPP-BQ?CB[8]-SCD)is successfully constructed by a bromophenylpyridine-tethered-bromoisoquinoline(BPP-BQ),cucurbit[8]uril(CB[8])and sulfonatedβ-cyclodextrin(SCD)via successive assembling way,exhibiting progressively enhanced green roomtemperature phosphorescence(RTP).The self-aggregates of BPP-BQ?CB[8]-SCD accommodate an energy acceptor rhodamine B(Rh B)to form a light-harvesting system(BPP-BQ?CB[8]-SCD@Rh B)with further enhanced yellow long-lifetime luminescence with large Stokes shift based on triplet-singlet F?rster resonance energy transfer(TS-FRET).Crucially,the introduction of a photoactive diarylethene achieves the long-lived photoluminescence of BPP-BQ?CB[8]-SCD@Rh B to be switched with the efficiency of up to98%through logically ordered lowering/enhancing RTP performance of the energy donor and intercepting/restoring TS-FRET pathway,when stimulated by host-vip competition and light illumination in sequence.Moreover,BPP-BQ?CB[8]-SCD@Rh B is evenly doped into polyvinyl alcohol or polyacrylamide to obtain high-performance luminescent films with long afterglow.The abovementioned logically ordered stimulus-switched long-lived emission enables the light-harvesting system in both solution and solid state to be applied in high-security-level information encryption and transformation,and anticounterfeiting.展开更多
The development of multi-stimuli-responsive luminescent system to address emerging demands is essential in anti-counterfeiting field.Herein,a photoswitchable system was reported,which was constructed from photoacid su...The development of multi-stimuli-responsive luminescent system to address emerging demands is essential in anti-counterfeiting field.Herein,a photoswitchable system was reported,which was constructed from photoacid sulfonato-merocyanine(MEH-D)serving as H+donor and diarylethene derivative(DAEA1)as acceptor.After capturing 2 equiv.HCl,the obtained fluorescent molecule DAE-A1-H showed solvatochromic property.Further on,benefiting from that MEH-D released protons and became a ring-closed isomer spiropyran(SP-D)under 440 nm irradiation,DAE-A1 was protonated,turning on fluorescence effect was realized in DAE-A1/MEH-D.In dark,a photo-activated reversible process was realized with SPD changed to MEH-D in situ system.In addition,the OF-DAE-A1-H/SP-D could efficiently and reversibly switch on/off its luminescence upon irradiation by UV–vis light.Significantly,the multi-stimuli-responsive system was successfully applied in logic gate and fluorescence ink,making it an efficient strategy for information encryption and decryption with higher security requirements.展开更多
In pursuit of low cost and long life for lithium-ion batteries in electric vehicles,the most promising strategy is to replace the commercial LiCoO_(2)with a high-energy-density Ni-rich cathode.However,the irreversible...In pursuit of low cost and long life for lithium-ion batteries in electric vehicles,the most promising strategy is to replace the commercial LiCoO_(2)with a high-energy-density Ni-rich cathode.However,the irreversible redox couples induce rapid capacity decay,poor long-term cycling life,vast gas evolution,and unstable structure transformations of the Ni-rich cathode,limiting its practical applications.Element doping has been considered as the most promising strategy for addressing these issues.However,the relationships between element doping functions and redox chemistry still remain confused.To clarify this connection,this review places the dynamic evolution of redox couples(Li^(*),Ni^(2+)/Ni^(3+)/Ni^(4+)-e^(-),O^(2-)/O^(n-)/O_(2)-e^(-))as the tree trunk.The material structure,degradation mechanisms,and addressing element doping strategies are considered as the tree branches.This comprehensive summary aims to provide an overview of the current understanding and progress of Ni-rich cathode materials.In the last section,promising strategies based on element doping functions are provided to encourage the practical application of Ni-rich cathodes.These strategies also offer a new approach for the development of other intercalated electrode materials in Na and K-based battery systems.展开更多
Adjustment of the sowing date is a widely used measure in rice production for adapting to high-temperature conditions.However,the impact of a delayed sowing date(DS)on rice quality may vary by variety and ecological c...Adjustment of the sowing date is a widely used measure in rice production for adapting to high-temperature conditions.However,the impact of a delayed sowing date(DS)on rice quality may vary by variety and ecological conditions.In this study,we conducted experiments using four different sowing dates,the conventional sowing date 1(CS1),CS2(10 d later than CS1),DS1(30 d later than CS1),and DS2(30 d later than CS2),and three rice varieties,i.e.,Yixiangyou 2115,Fyou 498,and Chuanyou 6203.This experiment was conducted at four sites in the Sichuan Basin in 2018 and 2019 to evaluate the infuence of DS on the pasting properties of rice,which are a proxy for the eating and cooking quality(ECQ).In DS1 and DS2,the rice had a signifcantly greater amylose content(AC)but a lower protein content(PC),peak viscosity(PKV),cool paste viscosity(CPV),and hot paste viscosity(HPV)than in CS1 and CS2.Moreover,except for CS2 and DS1 in 2018,DS1 and DS2 led to 2.15-11.19%reductions in breakdown viscosity(BDV)and 23.46-108.47%increases in setback viscosity(SBV).However,the infuence of DS on rice pasting properties varied by study site and rice variety.In 2019,DS1 and DS2 led to BDV reductions of 2.35-9.33,2.61-8.61,10.03-17.78,and 2.06-8.93%,and SBV increases of 2.32-60.93,63.74-144.24,55.46-91.63,and-8.28-65.37%at the Dayi,Anzhou,Nanbu,and Shehong(except for SBV in CS2 and DS1)sites,respectively.DS resulted in greater reductions in PKV,HPV,CPV,and BDV and greater increases in the AC and SBV for Yixiangyou 2115 than for Chuanyou 6203 and Fyou 498.The correlation analysis indicated that PKV and HPV were signifcantly and positively related to the mean,maximum,and minimum temperatures after heading.These temperatures must be greater than 25.9,31.2,and 22.3℃,respectively,to increase the relative BDV and reduce the relative SBV of rice,thereby enhancing ECQ.In conclusion,DS might contribute to a signifcant deterioration in ECQ in machine-transplanted rice in the Sichuan Basin.A mean temperature above 25.9℃ after heading is required to improve the ECQ of rice.展开更多
Herein,anthracene-pyridinium derivative(A1)is synthesized to assemble with amphiphilic sulfonatocalix[4]arene(SC4AD)with a porous cavity through electrostatic interaction,exhibiting enhanced fluorescence emission and ...Herein,anthracene-pyridinium derivative(A1)is synthesized to assemble with amphiphilic sulfonatocalix[4]arene(SC4AD)with a porous cavity through electrostatic interaction,exhibiting enhanced fluorescence emission and multipath fluorescence resonance energy transfer(FRET)with organic dyes(EY,Ni R or Cy5.5).In this nanoassembly,A1/SC4AD first transfers the energy to dye EY(first acceptor),and then delivers it to Ni R(second acceptor),which further transfers the energy to Cy5.5(third acceptor),accompanying with an emission ranging from 535 nm to 570 nm,then to 638 nm,and finally to near-infrared emission at 717 nm.Compared to one-step FRET(43.0%),the three-step FRET system shows higher energy transfer efficiency(FRET Ⅰ:84.9%,FRET Ⅱ:81.4%,FRET Ⅲ:66.9%).The donor/acceptor ratio is 3000(A1):20(EY):8(Ni R):5(Cy5.5),together with an antenna effect of 2.3.Additionally,diverse two-step cascade light-harvesting systems are successfully fabricated via tuning combination of dye acceptors.We believe that this multipath light-harvesting assembly will provide a direction for designing multiple sequential FRET and artificial light-harvesting systems.展开更多
Purely organic room-temperature phosphorescence(RTP)is current hotspot in the research fields of chemistry,biology,materials etc.Herein,we report that photo-thermal double response reversible ultralong RTP flexible el...Purely organic room-temperature phosphorescence(RTP)is current hotspot in the research fields of chemistry,biology,materials etc.Herein,we report that photo-thermal double response reversible ultralong RTP flexible elastic material with multicolor delayed fluorescence,which is constructed by 4-biphenylboronic acid(BOH),polyethylene glycol,2,2-bis(hydroxymethyl)propionic acid,isophorone diamine and isophorone diisocyanate copolymer.Importantly,the supramolecular phosphorescent elastomer not only exhibits extending RTP emission with a lifetime up to 1.21 s,but also gives a visible afterglow of 20 s via encapsulation of BOH unities by the deep cavities of hydroxypropyl-β-cyclodextrin(β-CD-HP)and in situ polymerization.Especially,after doping organic dyes(Fluorescein isothiocyanate,Sulforhodamine 101,Rhodamine B),supramolecular phosphorescent elastomer achieves multicolor delayed fluorescence realized by RTP energy transfer from phosphorescent donor to dye acceptors,which possesses reversible photo-thermal responsiveness and maintains high efficiency in delayed emission even after dozens of cycles.Present research provides a new approach for constructing multicolor delayed fluorescent supramolecular elastomers.展开更多
The electrochemical carbon dioxide reduction reaction(CO_(2)RR)to high value-added fuels or chemicals driven by the renewable energy is promising to alleviate global warming.However,the selective CO_(2)reduction to C_...The electrochemical carbon dioxide reduction reaction(CO_(2)RR)to high value-added fuels or chemicals driven by the renewable energy is promising to alleviate global warming.However,the selective CO_(2)reduction to C_(2)products remains challenge.Cu-based catalyst with the specific Cu^(0)and Cu^(+)sites is important to generate C_(2)products.This work used nitrogen(N)to tune amounts of Cu^(0)and Cu^(+)sites in Cu_(2)O catalysts and improve C_(2)-product conversion.The controllable Cu^(0)/Cu^(+)ratio of Cu_(2)O catalyst from 0.16 to 15.19 was achieved by adjusting the N doping amount using NH3/Ar plasma treatment.The major theme of this work was clarifying a volcano curve of the ethylene Faraday efficiency as a function of the Cu^(0)/Cu^(+)ratio.The optimal Cu^(0)/Cu^(+)ratio was determined as 0.43 for selective electroreduction CO_(2)to ethylene.X-ray spectroscopy and density functional theory(DFT)calculations were employed to elucidate that the strong interaction between N and Cu increased the binding energy of N–Cu bond and stabilize Cu^(+),resulting in a 92.3%reduction in the potential energy change for^(∗)CO-^(∗)CO dimerization.This study is inspiring in designing high performance electrocatalysts for CO_(2)conversion.展开更多
[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous ...[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.展开更多
Garnet-based all-solid-state lithium batteries(ASSLBs)were considered as the most promising energy storage device due to their high energy density and good safety.However,interface problems caused by impurities such a...Garnet-based all-solid-state lithium batteries(ASSLBs)were considered as the most promising energy storage device due to their high energy density and good safety.However,interface problems caused by impurities such as Li_(2)CO_(3) on the surface still hinder the practical application of garnet-based ASSLBs.Here,we use a simple ultrasonic spraying method to coat SiO_(2) on the Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)surface,and in-situ construct Li_(4)SiO_(4)/Li_(2) O/Li_(2)1 Si5 nano-multifunctional interlayer through the high-temperature conversion reaction of SiO_(2).Experiments and density functional theory(DFT)calculations demonstrate that the introduced Li_(4)SiO_(4)/Li_(2) O/Li_(2)1 Si_(5) nano-multifunctional interlayer at the LLZTO/Li interface can significantly improve the air stability and interface contact of LLZTO/Li.As a result,the interface impedance of Li/SiO_(2)@LLZTO/Li was reduced to 24.2Ωcm^(-2),and it can operate stably over 2400 and 1000 h at current densities of 0.05 and 0.2 mA cm^(−2),respectively.The full cell assembled with LiFePO_(4)(LFP)as cathode(Li/SiO_(2)@LLZTO/LFP)also exhibits excellent cycling performance(capacity retention rate of 95%after 50 cycles at 0.1 C)and rate performance(140 mAh g^(−1) at 0.1 C and 107 mAh g−1 at 1 C).This research provides a strategy to improve interface problems and achieve dendrite-free ASSLBs through in-situ transformation constructed nano-multifunctional interlayers.展开更多
基金Supported by Project of National Natural Science Foundation(81360524&81660701)Natural Science Foundation Project of Guangxi(2016 GXNSFAA380148&2014GXNSFAA118208)+1 种基金Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6)Program of Scientific Research Third-level Laboratory "Chinese(Zhuang) Medicine Chemical and Quality Analysis Laboratory" of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 2009[No.21])
文摘[Objectives] To study the differences in the effects of Rheum officinale and wine prepared Rheum palmatum Dachengqi Decoction(DCQ) on laxative effect of mice.[Methods]First,72 mice were randomly divided into positive control group,blank control group,6 and10 g/kg dose R.officinale DCQ groups,6 and 10 g/kg dose wine prepared R.palmatum DCQ groups.After administration at the dose of20 m L/kg,metabolic cage method and carbon powder propulsion were used to conduct Na+-K+-ATPasc activity experiment,and the comparison was made for the differences in the normal laxative effect of R.officinale and wine prepared R.palmatum on DCQ,the carbon powder propulsion rate,and Na+-K+-ATPasc activit.[Results]There are differences in the laxative effect of R.officinale and wine prepared R.palmatum on DCQ,but their differences in changes are,to some extent,connected with R.officinale and wine prepared R.palmatum.From comparison of R.officinale DCQ group,wine prepared R.palmatum DCQ group,blank control group,and positive control group,it is found that there are differences in the normal laxative effect of normal mice,carbon powder propulsion,and inhibition of Na+-K+-ATPase activity.In addition,the comparison of drug administration groups indicates that the differences in the effects of R.officinale DCQ group are most significant,and there are significant differences in the 6 and 10 g/kg groups.[Conclusions]There are certain differences in the influence of R.officinale and wine prepared R.palmatum Dachengqi Decoction on normal laxative effect of mice,which may be connected with the fact that changes in main chemical components of king drugs R.officinale and wine prepared R.palmatum lead to changes in the dissolution amount of anthraquinones in the compound Dachengqi Decoction.
基金supported by the Guizhou Provincial Basic Research Program(Natural Science)Youth Guidance Project{Qian Kehe Foundation-[2024]Youth 307}。
文摘In both Traditional Chinese Medicine(TCM)and modern medicine,they agree that the integrity and healthy structure of the vascular endothelium are essential for normal hemodynamics.Damage to the vascular endothelium can quickly activate the extrinsic coagulation pathway by triggering the tissue factor(TF)and lead to coagulation.This damage,along with a loss of anticoagulant properties through antithrombinⅢ(ATⅢ),TF pathway inhibitors,and the protein C system,can result in a hypercoagulable state and even thrombosis.Hypercoagulability is not only a common feature of many cancers but also an important factor promoting tumor development and metastasis,which corresponds to the TCM theory of“blood stasis leading to tumors.”The pharmacological effects of heparin and aspirin have similarities with TCM's“activating blood circulation and removing blood stasis”theory in improving blood circulation,treating related diseases,and their anti-inflammatory effects.
基金supported by the National Natural Science Foundations of China(Grant No.:82204584)Liaoning Provincial Science and Technology Projects,China(Project No.:2021JH1/10400055).
文摘Natural products(NPs)have historically been a fundamental source for drug discovery.Yet the complex nature of NPs presents substantial challenges in pinpointing bioactive constituents,and corresponding targets.In the present study,an innovative natural product virtual screening-interaction-phenotype(NP-VIP)strategy that integrates virtual screening,chemical proteomics,and metabolomics to identify and validate the bioactive targets of NPs.This approach reduces false positive results and enhances the efficiency of target identification.Salvia miltiorrhiza(SM),a herb with recognized therapeutic potential against ischemic stroke(IS),was used to illustrate the workflow.Utilizing virtual screening,chemical proteomics,and metabolomics,potential therapeutic targets for SM in the IS treatment were identified,totaling 29,100,and 78,respectively.Further analysis via the NP-VIP strategy highlighted five high-confidence targets,including poly[ADP-ribose]polymerase 1(PARP1),signal transducer and activator of transcription 3(STAT3),amyloid precursor protein(APP),glutamate-ammonia ligase(GLUL),and glutamate decarboxylase 67(GAD67).These targets were subsequently validated and found to play critical roles in the neuroprotective effects of SM.The study not only underscores the importance of SM in treating IS but also sets a precedent for NP research,proposing a comprehensive approach that could be adapted for broader pharmacological explorations.
基金Project supported by the National Natural Science Foundation of China(No.31271848)the Foundation of Fuli Institute of Food Science of Zhejiang University(No.KY201404),China
文摘Turmeric residue（TR）, containing residual levels of curcumin, is a solid by-product waste generated after the extraction and separation of curcumin from turmeric root. A feeding trial was conducted to evaluate the effects of TR on the survival of Chinese soft-shelled turtles（SSTs）, Pelodiscus sinensis, under a high ambient temperature. A total of 320 female SSTs were assigned randomly to two diets： basal diet（the control group, n=160） and an interventional diet supplemented with 10% TR（the TR group, n=160）. Our results demonstrated that supplementation of TR increased the SST survival rate by 135.5%, and superoxide dismutase（SOD） activity of SST liver by 112.8%, and decreased the malondialdehyde（MDA） content of SST liver by 36.4%, compared to the control group. The skin of the SST fed TR showed a golden color. High-performance liquid chromatography（HPLC） analysis indicated that the concentrations of curcumin in TR and the skin of the SST fed TR were（1.69±0.30） and（0.14±0.03） μg/g, respectively. Our observation suggests that supplementation of TR increased the survival rate of SST under high ambient temperatures. We speculated that the increased survival rate and tolerance at the high ambient temperature were associated with the anti-oxidation activity of curcumin from TR. Moreover, curcumin in TR could be deposited in SST skin, which made it more favored in the market of China. Our findings provide new knowledge and evidence to effectively reuse TR as a feed additive in animal and aquatic farming.
基金supported by Diabetes Talent Research Project of China,International Medical Foundation 2019(No.2018-N-1).
文摘Background The molecular mechanisms of early-onset multigenerational diabetes remain unknown.This study aimed to investigate the clinical and genetic characteristics of early-onset diabetes involving at least two consecutive generations.Methods From 1296 inpatients with diabetes,we selected individuals who were≤30 years of age and who were clinically suspected of having familial monogenic diabetes.Clinical data were collected from the probands and their family members.Whole-exome sequencing(WES)was used to identify possible causal variants for diabetes.Candidate pathogenic variants were verified by Sanger sequencing,assessed for cosegregation in family members,and evaluated on the basis of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology(ACMG/AMP)guidelines.Moreover,missense and synonymous variants were subjected to in silico pathogenicity prediction via MutationTaster and PolyPhen-2.RNAfold was used to predict RNA structural alterations for synonymous variants.Results Twenty-five early-onset diabetes patients with a history of familial diabetes were enrolled.Pathogenic/likely pathogenic variants(p.Gly292fs in HNF1A,p.Gly245Argfs*22 in PDX1,p.Asp329His in KCNJ11,p.Leu734Phe and p.Val606Gly in WFS1)were detected in four patients,who were diagnosed accurately and treated with reasonable hypoglycemic agents based on genetic testing results.The variants of uncertain significance(ABCC8 c.3039 G>A(p.Ser1013=Ser),MAPK8IP1 p.Gln144_Gly145insSerGln,and TBC1D4 p.Arg1249Trp)were identified in three probands.Conclusion Patients with early-onset diabetes involving at least two consecutive generations may harbor genetic variants.Genetic testing in this population enables precision diagnosis,informs individualized treatment,and facilitates genetic counseling.
基金the National Natural Science Foundation of China(Nos.21801063,22305070 and U20041101)the Top-Notch Talents Program of Henan Agricultural University(Nos.30501049 and 30501032)for financial support。
文摘Herein,a ternary supramolecular assembly(BPP-BQ?CB[8]-SCD)is successfully constructed by a bromophenylpyridine-tethered-bromoisoquinoline(BPP-BQ),cucurbit[8]uril(CB[8])and sulfonatedβ-cyclodextrin(SCD)via successive assembling way,exhibiting progressively enhanced green roomtemperature phosphorescence(RTP).The self-aggregates of BPP-BQ?CB[8]-SCD accommodate an energy acceptor rhodamine B(Rh B)to form a light-harvesting system(BPP-BQ?CB[8]-SCD@Rh B)with further enhanced yellow long-lifetime luminescence with large Stokes shift based on triplet-singlet F?rster resonance energy transfer(TS-FRET).Crucially,the introduction of a photoactive diarylethene achieves the long-lived photoluminescence of BPP-BQ?CB[8]-SCD@Rh B to be switched with the efficiency of up to98%through logically ordered lowering/enhancing RTP performance of the energy donor and intercepting/restoring TS-FRET pathway,when stimulated by host-vip competition and light illumination in sequence.Moreover,BPP-BQ?CB[8]-SCD@Rh B is evenly doped into polyvinyl alcohol or polyacrylamide to obtain high-performance luminescent films with long afterglow.The abovementioned logically ordered stimulus-switched long-lived emission enables the light-harvesting system in both solution and solid state to be applied in high-security-level information encryption and transformation,and anticounterfeiting.
基金financially supported by Natural Science Foundation of Shandong Province(Nos.ZR2022QB061,2022KJ181)National Key R&D Program of China(No.2023YFD1700903)。
文摘The development of multi-stimuli-responsive luminescent system to address emerging demands is essential in anti-counterfeiting field.Herein,a photoswitchable system was reported,which was constructed from photoacid sulfonato-merocyanine(MEH-D)serving as H+donor and diarylethene derivative(DAEA1)as acceptor.After capturing 2 equiv.HCl,the obtained fluorescent molecule DAE-A1-H showed solvatochromic property.Further on,benefiting from that MEH-D released protons and became a ring-closed isomer spiropyran(SP-D)under 440 nm irradiation,DAE-A1 was protonated,turning on fluorescence effect was realized in DAE-A1/MEH-D.In dark,a photo-activated reversible process was realized with SPD changed to MEH-D in situ system.In addition,the OF-DAE-A1-H/SP-D could efficiently and reversibly switch on/off its luminescence upon irradiation by UV–vis light.Significantly,the multi-stimuli-responsive system was successfully applied in logic gate and fluorescence ink,making it an efficient strategy for information encryption and decryption with higher security requirements.
基金supported by the National Natural Science Foundation of China(22209055)the China Postdoctoral Science Foundation(2022M721330)+2 种基金the Foshan Postdoctoral Science Foundation(X221081MS210)the Innovation Team of Universities of Guangdong Province(2022KCXTD030)the“Targeted Technology Innovation Initiative”Project at the Foshan National Institute of Innovation(JBGS2024002)。
文摘In pursuit of low cost and long life for lithium-ion batteries in electric vehicles,the most promising strategy is to replace the commercial LiCoO_(2)with a high-energy-density Ni-rich cathode.However,the irreversible redox couples induce rapid capacity decay,poor long-term cycling life,vast gas evolution,and unstable structure transformations of the Ni-rich cathode,limiting its practical applications.Element doping has been considered as the most promising strategy for addressing these issues.However,the relationships between element doping functions and redox chemistry still remain confused.To clarify this connection,this review places the dynamic evolution of redox couples(Li^(*),Ni^(2+)/Ni^(3+)/Ni^(4+)-e^(-),O^(2-)/O^(n-)/O_(2)-e^(-))as the tree trunk.The material structure,degradation mechanisms,and addressing element doping strategies are considered as the tree branches.This comprehensive summary aims to provide an overview of the current understanding and progress of Ni-rich cathode materials.In the last section,promising strategies based on element doping functions are provided to encourage the practical application of Ni-rich cathodes.These strategies also offer a new approach for the development of other intercalated electrode materials in Na and K-based battery systems.
基金supported by the National Natural Science Foundation of China(U20A2022 and 32372217)the National Key Research and Development Program of China(2022YFD2300700)the Free Exploration Program of State Key Laboratory of Crop Gene Exploration and Utilization in Sichuan Basin,China(SKL-ZY202216)。
文摘Adjustment of the sowing date is a widely used measure in rice production for adapting to high-temperature conditions.However,the impact of a delayed sowing date(DS)on rice quality may vary by variety and ecological conditions.In this study,we conducted experiments using four different sowing dates,the conventional sowing date 1(CS1),CS2(10 d later than CS1),DS1(30 d later than CS1),and DS2(30 d later than CS2),and three rice varieties,i.e.,Yixiangyou 2115,Fyou 498,and Chuanyou 6203.This experiment was conducted at four sites in the Sichuan Basin in 2018 and 2019 to evaluate the infuence of DS on the pasting properties of rice,which are a proxy for the eating and cooking quality(ECQ).In DS1 and DS2,the rice had a signifcantly greater amylose content(AC)but a lower protein content(PC),peak viscosity(PKV),cool paste viscosity(CPV),and hot paste viscosity(HPV)than in CS1 and CS2.Moreover,except for CS2 and DS1 in 2018,DS1 and DS2 led to 2.15-11.19%reductions in breakdown viscosity(BDV)and 23.46-108.47%increases in setback viscosity(SBV).However,the infuence of DS on rice pasting properties varied by study site and rice variety.In 2019,DS1 and DS2 led to BDV reductions of 2.35-9.33,2.61-8.61,10.03-17.78,and 2.06-8.93%,and SBV increases of 2.32-60.93,63.74-144.24,55.46-91.63,and-8.28-65.37%at the Dayi,Anzhou,Nanbu,and Shehong(except for SBV in CS2 and DS1)sites,respectively.DS resulted in greater reductions in PKV,HPV,CPV,and BDV and greater increases in the AC and SBV for Yixiangyou 2115 than for Chuanyou 6203 and Fyou 498.The correlation analysis indicated that PKV and HPV were signifcantly and positively related to the mean,maximum,and minimum temperatures after heading.These temperatures must be greater than 25.9,31.2,and 22.3℃,respectively,to increase the relative BDV and reduce the relative SBV of rice,thereby enhancing ECQ.In conclusion,DS might contribute to a signifcant deterioration in ECQ in machine-transplanted rice in the Sichuan Basin.A mean temperature above 25.9℃ after heading is required to improve the ECQ of rice.
基金financially supported by the National Natural Science Foundation of China(No.22131008)。
文摘Herein,anthracene-pyridinium derivative(A1)is synthesized to assemble with amphiphilic sulfonatocalix[4]arene(SC4AD)with a porous cavity through electrostatic interaction,exhibiting enhanced fluorescence emission and multipath fluorescence resonance energy transfer(FRET)with organic dyes(EY,Ni R or Cy5.5).In this nanoassembly,A1/SC4AD first transfers the energy to dye EY(first acceptor),and then delivers it to Ni R(second acceptor),which further transfers the energy to Cy5.5(third acceptor),accompanying with an emission ranging from 535 nm to 570 nm,then to 638 nm,and finally to near-infrared emission at 717 nm.Compared to one-step FRET(43.0%),the three-step FRET system shows higher energy transfer efficiency(FRET Ⅰ:84.9%,FRET Ⅱ:81.4%,FRET Ⅲ:66.9%).The donor/acceptor ratio is 3000(A1):20(EY):8(Ni R):5(Cy5.5),together with an antenna effect of 2.3.Additionally,diverse two-step cascade light-harvesting systems are successfully fabricated via tuning combination of dye acceptors.We believe that this multipath light-harvesting assembly will provide a direction for designing multiple sequential FRET and artificial light-harvesting systems.
基金financially supported by the National Natural Science Foundation of China(No.22131008)。
文摘Purely organic room-temperature phosphorescence(RTP)is current hotspot in the research fields of chemistry,biology,materials etc.Herein,we report that photo-thermal double response reversible ultralong RTP flexible elastic material with multicolor delayed fluorescence,which is constructed by 4-biphenylboronic acid(BOH),polyethylene glycol,2,2-bis(hydroxymethyl)propionic acid,isophorone diamine and isophorone diisocyanate copolymer.Importantly,the supramolecular phosphorescent elastomer not only exhibits extending RTP emission with a lifetime up to 1.21 s,but also gives a visible afterglow of 20 s via encapsulation of BOH unities by the deep cavities of hydroxypropyl-β-cyclodextrin(β-CD-HP)and in situ polymerization.Especially,after doping organic dyes(Fluorescein isothiocyanate,Sulforhodamine 101,Rhodamine B),supramolecular phosphorescent elastomer achieves multicolor delayed fluorescence realized by RTP energy transfer from phosphorescent donor to dye acceptors,which possesses reversible photo-thermal responsiveness and maintains high efficiency in delayed emission even after dozens of cycles.Present research provides a new approach for constructing multicolor delayed fluorescent supramolecular elastomers.
基金supported by“Pioneer”and“Leading Goose”R&D Program of Zhejiang(Nos.2022C03146 and 2023C03017)the National Natural Science Foundation of China(Nos.U23A20677 and 22022610)+1 种基金Zhejiang Provincial Natural Science Foundation of China(No.LDT23E06015B06)the National Funded Postdoctoral Researcher Program of China(No.GZC20232363).
文摘The electrochemical carbon dioxide reduction reaction(CO_(2)RR)to high value-added fuels or chemicals driven by the renewable energy is promising to alleviate global warming.However,the selective CO_(2)reduction to C_(2)products remains challenge.Cu-based catalyst with the specific Cu^(0)and Cu^(+)sites is important to generate C_(2)products.This work used nitrogen(N)to tune amounts of Cu^(0)and Cu^(+)sites in Cu_(2)O catalysts and improve C_(2)-product conversion.The controllable Cu^(0)/Cu^(+)ratio of Cu_(2)O catalyst from 0.16 to 15.19 was achieved by adjusting the N doping amount using NH3/Ar plasma treatment.The major theme of this work was clarifying a volcano curve of the ethylene Faraday efficiency as a function of the Cu^(0)/Cu^(+)ratio.The optimal Cu^(0)/Cu^(+)ratio was determined as 0.43 for selective electroreduction CO_(2)to ethylene.X-ray spectroscopy and density functional theory(DFT)calculations were employed to elucidate that the strong interaction between N and Cu increased the binding energy of N–Cu bond and stabilize Cu^(+),resulting in a 92.3%reduction in the potential energy change for^(∗)CO-^(∗)CO dimerization.This study is inspiring in designing high performance electrocatalysts for CO_(2)conversion.
基金Supported by Agricultural Science and Technology Innovation Fund of Hunan Province(XCNZ[2021]No.15)Loudi Science and Technology Innovation Program(LKF[2022]29)+1 种基金Applied Characteristic Discipline Construction Project of Hunan Province:Plant ProtectionPostgraduate Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2022Y12).
文摘[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.
基金The Key Research and Development Project of Hainan Province(No.ZDYF2022SHFZ093)sHainan Provincial Natural Science Foundation of China(No.120RC455)+1 种基金the Natural Science Foundation of China(No.61901142)the Program of Hainan Association for Science and Technology Plans to Youth R&D Innovation(No.QCXM202014)are acknowledged.
文摘Garnet-based all-solid-state lithium batteries(ASSLBs)were considered as the most promising energy storage device due to their high energy density and good safety.However,interface problems caused by impurities such as Li_(2)CO_(3) on the surface still hinder the practical application of garnet-based ASSLBs.Here,we use a simple ultrasonic spraying method to coat SiO_(2) on the Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)surface,and in-situ construct Li_(4)SiO_(4)/Li_(2) O/Li_(2)1 Si5 nano-multifunctional interlayer through the high-temperature conversion reaction of SiO_(2).Experiments and density functional theory(DFT)calculations demonstrate that the introduced Li_(4)SiO_(4)/Li_(2) O/Li_(2)1 Si_(5) nano-multifunctional interlayer at the LLZTO/Li interface can significantly improve the air stability and interface contact of LLZTO/Li.As a result,the interface impedance of Li/SiO_(2)@LLZTO/Li was reduced to 24.2Ωcm^(-2),and it can operate stably over 2400 and 1000 h at current densities of 0.05 and 0.2 mA cm^(−2),respectively.The full cell assembled with LiFePO_(4)(LFP)as cathode(Li/SiO_(2)@LLZTO/LFP)also exhibits excellent cycling performance(capacity retention rate of 95%after 50 cycles at 0.1 C)and rate performance(140 mAh g^(−1) at 0.1 C and 107 mAh g−1 at 1 C).This research provides a strategy to improve interface problems and achieve dendrite-free ASSLBs through in-situ transformation constructed nano-multifunctional interlayers.
