Background: Overconditioned dairy cows are susceptible to excessive lipolysis and increased insulin resistance during the transition period.The associations among body fat reserve,insulin resistance,and lipolysis in a...Background: Overconditioned dairy cows are susceptible to excessive lipolysis and increased insulin resistance during the transition period.The associations among body fat reserve,insulin resistance,and lipolysis in adipose tissues(AT) remain to be elucidated.Therefore,this study aimed to investigate whether excessive fat reserves influence the insulin signaling pathway in AT postpartum.Results: Twenty multiparous dairy cows were selected and assigned to one of two groups,according to prepartum body condition score(BCS): Control group(BCS = 3.0–3.5;n = 10) and Overconditioned group(BCS ≥ 4.0;n = 10).Blood samples were collected on days-14,-7,-4,-2,-1,0,1,2,4,7,and 14 relative to parturition.Subcutaneous AT were collected on day 2 following parturition for quantitative real-time polymerase chain reaction and western blot analyses.No differences were observed between the two groups in serum glucose,non-esterified fatty acids,β-hydroxybutyric acid,tumor necrosis factor(TNF)α,insulin,or leptin concentrations during the experimental period.Compared with the control cows,the overconditioned cows had lower serum triglyceride levels and higher adiponectin concentrations.In the AT postpartum,insulin receptor mRNA and protein levels were lower in the overconditioned cows than in the control cows,and no differences were found in glucose transporter 4 mRNA.Compared with the control cows,the overconditioned cows had lower mRNA levels of TNFα and higher mRNA levels of peroxisome proliferator-activated receptor gamma(PPARγ) in AT postpartum.The phosphorylated protein kinase B(AKT) content and phosphorylation rate of AKT were increased in the overconditioned cows compared with the control cows,which suggested that the downstream insulin signaling in AT was affected.Conclusions: In the present study,transition dairy cows with higher BCS did not show more fat mobilization.The changes of insulin signaling pathway in AT postpartum of overconditioned cows may be partly related to the expression of PPARγ and TNFα,and the secretion of adiponectin.展开更多
During a biological survey in July 2011, a pit viper was collected from the Yaoluoping Nature Reserve in the Dabie Mountains, Anhui, China. The pit viper’s the total length measured 836 mm with a dorsal ground color ...During a biological survey in July 2011, a pit viper was collected from the Yaoluoping Nature Reserve in the Dabie Mountains, Anhui, China. The pit viper’s the total length measured 836 mm with a dorsal ground color of yellowbrown, decorated by 56 dark-brown transverse bands. The 4thsupralabial squama of the new species is separated from subocular by two squamae, and the tip of tail is orange; this combination of characters distinguish it from other species of the genus Protobothrops. Based on the body proportions, number of squamae, body color pattern, and comparing the data of its life history with those of other species of Protobothrops, we herein describe it as a new species, belonging to the genus of Protobothrops.展开更多
Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigatio...Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigations,laboratory testing,and control measures.Methods:We collected serial blood samples throughout the patient’s hospitalization and documented the complete clinical progression from admission to discharge.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was employed to detect CHIKV nucleic acid,while Enzyme-Linked Immunosorbent Assay(ELISA)was used to test for CHIKV IgG and IgM antibodies.MinION nanopore sequencing was performed on blood samples to obtain the complete viral genome sequence.Phylogenetic analyses were subsequently constructed to determine the origin,genotype,and mutation profile of this imported case.Results:The qPCR analysis confirmed CHIKV presence in the patient’s serum samples.ELISA detected CHIKV IgG and IgM antibodies in blood samples collected on the fifth and ninth days after illness onset,respectively.Nanopore sequencing successfully generated the complete CHIKV genome sequence.Phylogenetic analysis demonstrated that the strain belonged to the East-Central-South-African lineage,consistent with the genotype identified from 190 cases in the local clustered Chikungunya fever(CHIKF)outbreak that occurred in Guangdong Province in July 2025.The strain showed 99.964% nucleotide identity(4 differential loci)with strain PX216392.1 detected in the current epidemic,and 99.9556% identity(5 differential loci)with PX236189.1.Conclusion:This is Anhui’s first imported CHIKV case,linked to the Guangdong outbreak.No local transmission or CHIKV-positive vector mosquitoes were detected.展开更多
The oriental white stork (Ciconia boyciana) is a threatened species, and their numbers are still in decline due tohabitat loss and poaching. China is a breeding and main wintering area for this animal and in recent ye...The oriental white stork (Ciconia boyciana) is a threatened species, and their numbers are still in decline due tohabitat loss and poaching. China is a breeding and main wintering area for this animal and in recent years someindividuals have been found breeding in wintering areas and at some stopover sites. These new breeding coloniesare an exciting sign, however, little is understood of the genetic structure of this species. Based on the analysis ofa 463-bp mitochondrial DNA (mtDNA) control region, we investigated the genetic structure and genetic diversity of66 wild oriental white storks from a Chinese population. We analyzed the sequences of 66 storks obtained in thisstudy and the data of 17 storks from a Japanese population. Thirty-seven different haplotypes were detected amongthe 83 samples. An analysis of molecular variance showed a significant population subdivision between the twopopulations (FST = 0.316, P < 0.05). However, the phylogenetic analysis revealed that the samples from the differentpopulations did not form separate clusters and that there were genetic exchanges between the two populations.Compared with the Japanese population, the Chinese population had a relatively higher genetic diversity with ahaplotype diversity (h ± SD) of 0.953 ± 0.013 and a nucleotide diversity (π ± SD) of 0.013 ± 0.007. The high haplotypediversity and low nucleotide diversity indicate that this population might be in a rapidly increasing period from asmall effective population. A neighbor-joining tree analysis indicated that genetic exchange had occurred betweenthe newly arisen southern breeding colony and the northern breeding colony wintering in the middle and lowerYangtze River floodplain. These results have important implications for the conservation of the oriental white storkpopulation in China.展开更多
基金supported by the National Key R&D Program of China(Project No.2017YFD0502200)the Programs for National Science Foundation of Beijing Municipality(Project No.6182005)
文摘Background: Overconditioned dairy cows are susceptible to excessive lipolysis and increased insulin resistance during the transition period.The associations among body fat reserve,insulin resistance,and lipolysis in adipose tissues(AT) remain to be elucidated.Therefore,this study aimed to investigate whether excessive fat reserves influence the insulin signaling pathway in AT postpartum.Results: Twenty multiparous dairy cows were selected and assigned to one of two groups,according to prepartum body condition score(BCS): Control group(BCS = 3.0–3.5;n = 10) and Overconditioned group(BCS ≥ 4.0;n = 10).Blood samples were collected on days-14,-7,-4,-2,-1,0,1,2,4,7,and 14 relative to parturition.Subcutaneous AT were collected on day 2 following parturition for quantitative real-time polymerase chain reaction and western blot analyses.No differences were observed between the two groups in serum glucose,non-esterified fatty acids,β-hydroxybutyric acid,tumor necrosis factor(TNF)α,insulin,or leptin concentrations during the experimental period.Compared with the control cows,the overconditioned cows had lower serum triglyceride levels and higher adiponectin concentrations.In the AT postpartum,insulin receptor mRNA and protein levels were lower in the overconditioned cows than in the control cows,and no differences were found in glucose transporter 4 mRNA.Compared with the control cows,the overconditioned cows had lower mRNA levels of TNFα and higher mRNA levels of peroxisome proliferator-activated receptor gamma(PPARγ) in AT postpartum.The phosphorylated protein kinase B(AKT) content and phosphorylation rate of AKT were increased in the overconditioned cows compared with the control cows,which suggested that the downstream insulin signaling in AT was affected.Conclusions: In the present study,transition dairy cows with higher BCS did not show more fat mobilization.The changes of insulin signaling pathway in AT postpartum of overconditioned cows may be partly related to the expression of PPARγ and TNFα,and the secretion of adiponectin.
基金funded by the Foundation for Young and Key Teachersand the Talent Cultivation Project of Anhui University(02203104/04)
文摘During a biological survey in July 2011, a pit viper was collected from the Yaoluoping Nature Reserve in the Dabie Mountains, Anhui, China. The pit viper’s the total length measured 836 mm with a dorsal ground color of yellowbrown, decorated by 56 dark-brown transverse bands. The 4thsupralabial squama of the new species is separated from subocular by two squamae, and the tip of tail is orange; this combination of characters distinguish it from other species of the genus Protobothrops. Based on the body proportions, number of squamae, body color pattern, and comparing the data of its life history with those of other species of Protobothrops, we herein describe it as a new species, belonging to the genus of Protobothrops.
基金Supported by the Anhui Provincial Health Science and Technology Project(AHWJ2024Aa20203 and AHWJ2024Aa20208)the Key Health Research Program of Anhui Province in 2024(AHWJ2024Aa10092)the Peak Discipline Project from the School of Public Health,Anhui Medical University(Collaborative Education Innovation Project)(2024GWXTYRZ005).
文摘Introduction:On August 11,2025,a suspected Chikungunya virus(CHIKV)case traveled from Foshan to Fuyang Xiguan Airport,Anhui Province.After symptom reporting on August 12,local CDC launched epidemiological investigations,laboratory testing,and control measures.Methods:We collected serial blood samples throughout the patient’s hospitalization and documented the complete clinical progression from admission to discharge.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was employed to detect CHIKV nucleic acid,while Enzyme-Linked Immunosorbent Assay(ELISA)was used to test for CHIKV IgG and IgM antibodies.MinION nanopore sequencing was performed on blood samples to obtain the complete viral genome sequence.Phylogenetic analyses were subsequently constructed to determine the origin,genotype,and mutation profile of this imported case.Results:The qPCR analysis confirmed CHIKV presence in the patient’s serum samples.ELISA detected CHIKV IgG and IgM antibodies in blood samples collected on the fifth and ninth days after illness onset,respectively.Nanopore sequencing successfully generated the complete CHIKV genome sequence.Phylogenetic analysis demonstrated that the strain belonged to the East-Central-South-African lineage,consistent with the genotype identified from 190 cases in the local clustered Chikungunya fever(CHIKF)outbreak that occurred in Guangdong Province in July 2025.The strain showed 99.964% nucleotide identity(4 differential loci)with strain PX216392.1 detected in the current epidemic,and 99.9556% identity(5 differential loci)with PX236189.1.Conclusion:This is Anhui’s first imported CHIKV case,linked to the Guangdong outbreak.No local transmission or CHIKV-positive vector mosquitoes were detected.
文摘The oriental white stork (Ciconia boyciana) is a threatened species, and their numbers are still in decline due tohabitat loss and poaching. China is a breeding and main wintering area for this animal and in recent years someindividuals have been found breeding in wintering areas and at some stopover sites. These new breeding coloniesare an exciting sign, however, little is understood of the genetic structure of this species. Based on the analysis ofa 463-bp mitochondrial DNA (mtDNA) control region, we investigated the genetic structure and genetic diversity of66 wild oriental white storks from a Chinese population. We analyzed the sequences of 66 storks obtained in thisstudy and the data of 17 storks from a Japanese population. Thirty-seven different haplotypes were detected amongthe 83 samples. An analysis of molecular variance showed a significant population subdivision between the twopopulations (FST = 0.316, P < 0.05). However, the phylogenetic analysis revealed that the samples from the differentpopulations did not form separate clusters and that there were genetic exchanges between the two populations.Compared with the Japanese population, the Chinese population had a relatively higher genetic diversity with ahaplotype diversity (h ± SD) of 0.953 ± 0.013 and a nucleotide diversity (π ± SD) of 0.013 ± 0.007. The high haplotypediversity and low nucleotide diversity indicate that this population might be in a rapidly increasing period from asmall effective population. A neighbor-joining tree analysis indicated that genetic exchange had occurred betweenthe newly arisen southern breeding colony and the northern breeding colony wintering in the middle and lowerYangtze River floodplain. These results have important implications for the conservation of the oriental white storkpopulation in China.
