This study investigates the efficacy of polysaccharides derived from Osmunda japonica Thunb in the context of immunoinflammation and elucidates its underlying mechanisms.The findings demonstrate that Osmunda japonica ...This study investigates the efficacy of polysaccharides derived from Osmunda japonica Thunb in the context of immunoinflammation and elucidates its underlying mechanisms.The findings demonstrate that Osmunda japonica Thunb polysaccharides(OP)significantly enhances microcirculation in vivo,facilitates immune regu-lation,and markedly promotes cell proliferation.Notably,the high-dose OP group exhibited a 45.18%inhibition rate on ear edema in mice,with a maximum vasodilatory effect of 16.72%.Additionally,after 24 h of OP stimulation of macrophages,the most pronounced promotion of activity was observed at 151.36%when the OP concentration reached 100μg/mL.OP also stimulates the secretion of nitric oxide(NO)and pro-inflammatory cytokines,including interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),prostaglandin E2(PGE2),and interleukin-1β(IL-1β),indicating its potent biological activity.Furthermore,OP promotes macrophage activation and NO release by activating the mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)signaling pathways,with particular emphasis on the NF-κB pathway,as well as c-Jun N-terminal kinase(JNK)and p38 MAPK within the MAPK pathway.These findings not only elucidate the mechanisms by which OP functions in the realm of immune inflammation but also provide a scientific foundation for the development of immune-regulating food products,thereby contributing to enhanced human health.展开更多
基金supported by a grant from the 2023 Jiangsu Province Industry-University-Research Cooperation Project(BY20230427)the Yancheng Science and Technology Planning Project(YCBK202250)the Yancheng Institute of Technology School-level Talent Project(xjr2021025).
文摘This study investigates the efficacy of polysaccharides derived from Osmunda japonica Thunb in the context of immunoinflammation and elucidates its underlying mechanisms.The findings demonstrate that Osmunda japonica Thunb polysaccharides(OP)significantly enhances microcirculation in vivo,facilitates immune regu-lation,and markedly promotes cell proliferation.Notably,the high-dose OP group exhibited a 45.18%inhibition rate on ear edema in mice,with a maximum vasodilatory effect of 16.72%.Additionally,after 24 h of OP stimulation of macrophages,the most pronounced promotion of activity was observed at 151.36%when the OP concentration reached 100μg/mL.OP also stimulates the secretion of nitric oxide(NO)and pro-inflammatory cytokines,including interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),prostaglandin E2(PGE2),and interleukin-1β(IL-1β),indicating its potent biological activity.Furthermore,OP promotes macrophage activation and NO release by activating the mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)signaling pathways,with particular emphasis on the NF-κB pathway,as well as c-Jun N-terminal kinase(JNK)and p38 MAPK within the MAPK pathway.These findings not only elucidate the mechanisms by which OP functions in the realm of immune inflammation but also provide a scientific foundation for the development of immune-regulating food products,thereby contributing to enhanced human health.
