Gossypium raimondii(2n=2x=26,D_(5)),an untapped wild species,is the putative progenitor of the D-subgenome of G.hirsutum(2n=4x=52,AD_(1)),an extensively cultivated species.Here,we developed a G.hirsutum(recipient)–G....Gossypium raimondii(2n=2x=26,D_(5)),an untapped wild species,is the putative progenitor of the D-subgenome of G.hirsutum(2n=4x=52,AD_(1)),an extensively cultivated species.Here,we developed a G.hirsutum(recipient)–G.raimondii(donor)introgression population to exploit the favorable QTLs/genes and mapped potential quantitative trait loci(QTLs)from wild cotton species.The introgression population consisted of 256 lines with an introgression rate of 52.33%for the G.raimondii genome.The introgression segment length range was 0.03–19.12 Mb,with an average of 1.22 Mb.The coverage of total introgression fragments from G.raimondii was 386.98 Mb.Further genome-wide association analysis(Q+K+MLM)and QTL mapping(RSTEP-LRT)identified 59 common QTLs,including 14 stable QTLs and six common QTL(co-QTL)clusters,and one hotspot of micronaire(MIC).The common QTLs for seed index all showed positive additive effects,while the common QTLs for boll weight all had negative additive effects,indicating that the linkage between seed index and boll weight could be broken.QTLs for lint percentage showed positive effects and could be beneficial for improving cotton yield.Most QTLs for fiber quality had negative additive effects,implying these QTLs were domesticated/improved in G.hirsutum.A few fiber quality QTLs showed positive additive effects,so they could be used to improve cotton fiber quality.The introgression lines developed could be useful for molecular marker-assisted breeding and mapping QTLs precisely for mining desirable genes from the wild species G.raimondii.Such genes can improve cultivated cotton in the future through a designbreeding approach.展开更多
TypeⅠinterferon(IFN)-mediated innate immune responses represent the first line of host defense against viral infection.However,the molecular mechanisms by which avian infuenza virus(AIV)inhibits typeⅠIFN production ...TypeⅠinterferon(IFN)-mediated innate immune responses represent the first line of host defense against viral infection.However,the molecular mechanisms by which avian infuenza virus(AIV)inhibits typeⅠIFN production in ducks are not well understood.Here,we frst found that the polymerase basic 2(PB2)protein of H5N1 subtype AIV inhibited the typeⅠIFN responses by targeting duck mitochondrial antiviral signaling protein(MAVS).We further demonstrated that H5N1-PB2 bound to theΔtransmembrane(ΔTM)domain of duck MAVS,and the polymerase basic 1(PB1)binding domain(PBD)and RNA binding nuclear import domain(RND)of H5N1-PB2 interacted with MAVS to inhibit typeⅠIFN expression in ducks.Collectively,our fndings contribute to understanding the molecular mechanism by which AIV proteins regulate the retinoic acid-inducible geneⅠ(RIG-Ⅰ)-like receptor(RLR)signaling pathway to evade host antiviral immune responses in ducks.展开更多
[Objectives]Farmland ginseng cultivation,as a sustainable alternative to traditional forest-clearing ginseng planting,requires systematic evaluation of soil optimization strategies.This study aimed to quantify the lin...[Objectives]Farmland ginseng cultivation,as a sustainable alternative to traditional forest-clearing ginseng planting,requires systematic evaluation of soil optimization strategies.This study aimed to quantify the linkage between soil improvement outcomes and ginseng(Panax ginseng)yield across five regions in Yanbian Korean Autonomous Prefecture.[Methods]Soil improvement trials were conducted using farmland soils,with forest soils as the baseline.Soil nutrient contents were measured via soil agrochemical analysis method using a continuous flow analyzer.Statistical approaches,including significance tests,correlation analysis,and regression analysis,were applied to identify key factors influencing yield.[Results]Ginseng yield exhibited a significant positive correlation with organic matter content and available phosphorus,but a negative correlation with electrical conductivity,ammonium nitrogen,and available potassium.Wangqing and Liucai regions achieved post-improvement yields equivalent to 94%and 88%of forest soil yields,respectively,demonstrating the highest soil similarity to forest ecosystems.[Conclusions]Region-specific soil improvement protocols in Wangqing and Liucai show high replicability and efficacy.These strategies can serve as benchmarks for sustainable farmland ginseng cultivation,minimizing ecological disruption while maintaining productivity.展开更多
Deforestation is one of the most serious environmental problems facing humankind.It continues to escalate rapidly across many regions of the world,thereby deteriorating the forest soil quality.This has prompted a larg...Deforestation is one of the most serious environmental problems facing humankind.It continues to escalate rapidly across many regions of the world,thereby deteriorating the forest soil quality.This has prompted a large number of field-based studies aimed at understanding the impacts of deforestation on soil properties.However,the lack of comprehensive meta-analyses that utilized these studies has limited our deeper understanding of how different soil properties,including the soil organic carbon(SOC)pool,respond to deforestation.To address this critical knowledge gap,we conducted a meta-analysis of 144 studies to explore the impacts of deforestation on soil chemical,physical,and biological properties,with special emphasis on the long-term changes in SOC,such as concentrations,stocks,and sequestration.The results revealed that deforestation significantly decreased soil organic matter,electrical conductivity,and base saturation by 52%,50%,and 98%,respectively.While deforestation increased soil total nitrogen content and decreased available phosphorus content by 51%and 99%,respectively,it resulted in slight decreases in some chemical properties,including soil pH(1%)and base cations(1%–13%).Deforestation significantly increased bulk density by 27%and soil erosion by 47%,but significantly decreased soil aggregate stability by 39%and saturated hydraulic conductivity by 63%.Soil microbial biomass C and N concentrations and enzyme activities were significantly decreased as a consequence of deforestation.Soil biological properties were much more affected by deforestation than soil physical and chemical properties.Regarding the SOC,the land use conversion from forest to pasture significantly increased SOC concentrations,stocks,and sequestration rates(11%–13%),whereas the land use conversions from forest to both plantation and cropland significantly decreased SOC concentrations,stocks,and sequestration rates(10%–43%).This observed decline in SOC accumulations decreased with increasing years after deforestation.The SOC dynamics following deforestation were predominantly regulated by microbial biomass concentrations,dehydrogenase activity,soil erosion,saturated hydraulic conductivity,aggregate stability,as well as concentrations of total organic carbon,total nitrogen,total phosphorus and organic matter.The present meta-analytical study provides compelling evidence that deforestation can induce profound changes in soil characteristics,including soil C contents,and has significant implications for soil health sustainability and climate change mitigation.展开更多
We successfully prepared a series of rare-earth doped borosilicate glasses using the melt-quenching method,and carefully investigated the luminescent properties and the spectral modulation of Tb/Eucodoped borosilicate...We successfully prepared a series of rare-earth doped borosilicate glasses using the melt-quenching method,and carefully investigated the luminescent properties and the spectral modulation of Tb/Eucodoped borosilicate glasses under UV(200-400 nm)excitation.The results show that the prepared samples have the characteristics of broadband response,excellent transparency and tunable luminescence.By adjusting the excitation wavelength,the emissions of Tb^(3+),Eu^(2+)and Eu^(3+)ions are observed,which exhibit yellow-green,blue,red color and multi-color even white emissions,respectively.Moreover,the energy transfer between Tb^(3+)and Eu^(3+)ions in the codoped glasses is confirmed.Tb^(3+)absorbs a large number of solar-blind light,transfers to Eu^(3+)and results in intense visible emission in a wide waveband range.This makes the Tb/Eu co-doped glass a desirable candidate for solar-blind light detections.The photodetection system was built and shows a strong and stable response to the UV light of 210-400 nm.Due to broad detection range,high sensitivity and stability,our results offer strong implications for the development of photodetection device for diverse applications.展开更多
Background:To comprehensively analyze the clinical characteristics of patients who underwent gastric endoscopic submucosal dissection(ESD)and explore the incidence and influencing factors of postoperative pain.Methods...Background:To comprehensively analyze the clinical characteristics of patients who underwent gastric endoscopic submucosal dissection(ESD)and explore the incidence and influencing factors of postoperative pain.Methods:The clinical data of patients who underwent gastric ESD at our center from 2009 to 2024 were retrospectively analyzed.Pain severity was assessed using a visual analogue scale,with a score≥4 defined as postoperative pain.Based on the presence or absence of postoperative pain,patients were divided into a pain group and a control group.Independent factors influencing postoperative pain were identified using multivariate logistic regression analysis.To control for confounding bias,patients in the case and control groups were matched by sex and lesion size,and the matched participants were further analyzed using a conditional logistic regression model.Results:In total,993 patients were analyzed.The incidence of postoperative pain was 9.1%(95%confidence interval[CI],7.3-11.1).In the univariate analysis,sex,operation duration,anesthesia method,intraoperative electrocoagulation,nasogastric tube placement,and postoperative vomiting were significantly associated with postoperative pain.Multivariate analysis identified eight independent factors:male sex(odds ratio[OR],0.61;95%CI,0.37-0.97;p=0.04),operation duration(OR,1.29;95%CI,1.03-1.63;p=0.02),protuberant lesions(OR,0.43;95%CI,0.26-0.71;p<0.01),antral lesions(OR,1.84;95%CI,1.10-3.05;p=0.01),intubation general anesthesia(OR,0.40;95%CI,0.22-0.72;p=0.002),intraoperative electrocoagulation(OR,0.32;95%CI,0.19-0.55;p<0.01),nasogastric tube placement(OR,2.005;95%CI,1.12-3.57;p=0.01),and postoperative vomiting(OR,3.24;95%CI,1.40-7.47;p=0.005).Conditional logistic regression analysis further identified diabetes mellitus(OR,2.50;95%CI,1.03-6.06;p=0.04).Conclusion:Female sex,diabetes mellitus,concave-type lesions,lesions in the gastric antrum,non-intubation general anesthesia,absence of intraoperative electrocoagulation,prolonged operation duration,nasogastric tube placement,and postoperative vomiting were independent factors associated with moderate to severe pain after gastric ESD.For patients at increased risk of postoperative pain,appropriate prophylactic and therapeutic measures during the perioperative period may effectively alleviate pain following gastric ESD.展开更多
Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to i...Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.展开更多
<strong>Background:</strong> Oncolytic herpes simplex virus (oHSV) have been proved effective and safe to treat tumors. Glycoprotein D (gD) has been engineered for targeting cancer cells and de-targeting n...<strong>Background:</strong> Oncolytic herpes simplex virus (oHSV) have been proved effective and safe to treat tumors. Glycoprotein D (gD) has been engineered for targeting cancer cells and de-targeting normal cells successfully, however, the effectiveness and safety of oHSVs still need to be improved. <strong>Method:</strong> Here we sequenced the DNA encoding gD of our recently isolated new strain HSV-1-LXMW and compared the gD amino acid sequence with the gDs of other 7 HSV-1 and 3 HSV-2 strains. <strong>Results:</strong> Phylogenetic analysis revealed that HSV-1-LXMW is evolutionarily close to HSV-1-Patton and -KOS strains. The gD amino acid sequence alignment identified 19 conserved and 8 variable regions. We further predicted 10 new motifs in HSV gD for the first time and identified motif differences in HSV-1 and HSV-2. We summarized the gD-engineered oHSVs and found that some of the newly identified gD motifs are actually functional. <strong>Conclusion:</strong> Our results shed light on HSV gD biology and provided new directions for future gD functional studies and engineering in order to make better oHSVs.展开更多
Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library...Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate(EMS). F2 and F2:3generations produced from crosses of M8008 × YN15(MY) and M8008 × SJZ54(MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height(PH), spike length(SL),fertile spikelet number per spike(FSS), grain width(GW), grain length(GL), GL/GW ratio(GLW), and thousand-grain weight(TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat(SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci(QTLs) forming QTL clusters(one in MY and two in MS) were detected. The QTL clusters coinciding with(MY population) or near(MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density(FFD), and TGW and to increases in GLW. The cluster in the wmc25–barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant(SN), and SL.展开更多
With the large-scale commercial launch of fifth generation(5G)mobile network,the development of new services and applications catering to the year 2030,along with the deep convergence of information,communication,and ...With the large-scale commercial launch of fifth generation(5G)mobile network,the development of new services and applications catering to the year 2030,along with the deep convergence of information,communication,and data technologies(ICDT),and the lessons and experiences from 5G practice will drive the evolution of the next generation of mobile networks.This article surveys the history and driving forces of the evolution of the mobile network architecture and proposes a logical function architecture for sixth generation(6G)mobile network.The proposed 6G network architecture is termed SOLIDS(related to the following basic features:soft,on-demand fulfillment,lite,native intelligence,digital twin,and native security),which can support self-generation,self-healing,self-evolution,and self-immunity without human involvement and address the primary issues in the legacy 5G network(e.g.,high cost,high power consumption,and highly complicated operation and maintenance),significantly well.展开更多
OBJECTIVE:To verify the effects of scraping therapy on the weightlifting ability by measuring the subjective sensation,and changes of biomarkers.METHODS:Five students,who have been trained for 3 years in a sport schoo...OBJECTIVE:To verify the effects of scraping therapy on the weightlifting ability by measuring the subjective sensation,and changes of biomarkers.METHODS:Five students,who have been trained for 3 years in a sport school in China were participated in this study.A course of scraping therapy was applied to intervene during the normal 7-week of weightlifting training programme.The ability of weightlifting,the scale of rating perceived exertion and serum biochemical markers were measured before and after the intervention.RESULTS:Scraping therapy caused a significant increase in weightlifting ability(P<0.05).The level of rating perceived exertion remained stable with the increase in the training volume.Immuno-globulinA was significantly increased(P<0.05),and creatine kinase and blood urea nitrogen were significantly decreased(P<0.05).No significant changes were observed in white blood cell,neutrophil,and testosterone.CONCLUSION:Scraping therapy may facilitate weightlifting ability mainly by decreasing weight sensation and improving serum biochemical parameters.展开更多
Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two deca...Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of bacteria.The discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial agents.For the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS systems.This review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.展开更多
Lithium-selenium(Li-Se)battery has attracted growing attention.Nevertheless,its practical application is still impeded by the shuttle effect of the formed polyselenides.Herein,we report in-situ hydrothermal weaving th...Lithium-selenium(Li-Se)battery has attracted growing attention.Nevertheless,its practical application is still impeded by the shuttle effect of the formed polyselenides.Herein,we report in-situ hydrothermal weaving the three-dimensional(3 D)highly conductive hierarchically interconnected nanoporous web by threading microporous metal organic framework MIL-68(Al)crystals onto multi-walled carbon nanotubes(MWCNTs).Such 3 D hierarchically nanoporous web(3 D MIL-68(Al)@MWCNTs web)with a very high surface area,a large amount of micropores,electrical conductivity and elasticity strongly traps the soluble polyselenides during the electrochemical reaction and significantly facilitates lithium ion diffusion and electron transportation.Molecular dynamic calculation confirmed the strong affinity of MIL-68(Al)for the adsorption of polyselenides,quite suitable for Li-Se battery.Their hexahedral channels(1.56 nm)are more efficient for the confinement of polyselenides and for the diffusion of electrolytes compared to their smaller triangular channels(0.63 nm).All these excellent characteristics of 3 D MIL-68(Al)@MWCNTs web with suitable confinement of a large amount of selenium and the conductive linkage between MIL-68(Al)host by MWCNTs result in a high capacity of 453 m Ah/g at 0.2 C with 99.5%coulombic efficiency after 200 cycles with significantly improved cycle stability and rate performance.The 3 D MIL-68(Al)@MWCNTs web presents a good performance in Li-Se battery in term of the specific capacity and cycling stability and also in terms of rate performance compared with all the metal-organic framework(MOF)based or MOF derived porous carbons used in Li-Se battery.展开更多
Objective:To observe the clinical effect difference between abdominal acupuncture combined with megestrol acetate and megestrol acetate for advanced cancer-related anorexia.Methods:A total of 60 patients with advanced...Objective:To observe the clinical effect difference between abdominal acupuncture combined with megestrol acetate and megestrol acetate for advanced cancer-related anorexia.Methods:A total of 60 patients with advanced cancer-related anorexia were randomly divided into an abdominal acupuncture combined with oral megestrol acetate treatment group(group A)and oral megestrol acetate treatment group(group B),with 30 cases in each group.Both groups were given nutritional assessment,nutritional supplementation,improvement of constipation,correction of electrolyte disturbance and other general treatments.In addition,group B was given another 160 mg megestrol acetate dispersible tablet once a day.Group A was treated with abdominal acupuncture on the basis of group B treatment,5 times a week,and both the groups were treated for 2 weeks.The appetite visual analog scale(VAS),Karnofsky(KPS)score,and body weight of the two groups were observed before and after treatment,and the clinical efficacy was evaluated.Results:After treatment,the appetite VAS in the both groups were lower than those before treatment,the Karnofsky(KPS)scores were higher than those before treatment,and the differences were statistically significant(both P<0.05).The differences of appetite VAS and KPS score before and after treatment in group A were higher than those of group B,and the differences were statistically significant(both P<0.05).After treatment,the body weights of the two groups were higher than those before treatment,and the difference of group A before and after treatment was higher than that of group B,but the difference was not statistically significant(P>0.05).The effective rate of group A was 76.7%and that of group B was 50%,the difference was statistically significant(P<0.05).Conclusion:Abdominal acupuncture combined with oral megestrol acetate and oral megestrol acetate both can effectively improve the appetite and functional status of patients with advanced cancer-related anorexia.Abdominal acupuncture can improve the curative effect,which is better than that of western medication alone,providing a new idea for the treatment of advanced cancer-related anorexia in the future.展开更多
A splicing mutation in VPS4B can cause dentin dysplasia type I(DD-I),a hereditary autosomal-dominant disorder characterized by rootless teeth,the etiology of which is genetically heterogeneous.In our study,dental foll...A splicing mutation in VPS4B can cause dentin dysplasia type I(DD-I),a hereditary autosomal-dominant disorder characterized by rootless teeth,the etiology of which is genetically heterogeneous.In our study,dental follicle cells(DFCs)were isolated and cultured from a patient with DD-I and compared with those from an age-matched,healthy control.In a previous study,this DD-I patient was confirmed to have a loss-of-function splicing mutation in VPS4B(IVS7+46C>G).The results from this study showed that the isolated DFCs were vimentin-positive and CK14-negative,indicating that the isolated cells were derived from the mesenchyme.DFCs harboring the VPS4B mutation had a significantly higher proliferation rate from day 3 to day 8 than control DFCs,indicating that VPS4B is involved in cell proliferation.The cells were then replenished with osteogenic medium to investigate how the VPS4B mutation affected osteogenic differentiation.Induction of osteogenesis,detected by alizarin red and alkaline phosphatase staining in vitro,was decreased in the DFCs from the DD-I patient compared to the control DFCs.Furthermore,we also found that the VPS4B mutation in the DD-I patient downregulated the expression of osteoblast-related genes,such as ALP,BSP,OCN,RUNX2,and their encoded proteins.These outcomes confirmed that the DD-I-associated VPS4B mutation could decrease the capacity of DFCs to differentiate during the mineralization process and may also impair physiological root formation and bone remodeling.This might provide valuable insights and implications for exploring the pathological mechanisms underlying DD-I root development.展开更多
基金funded by the National Key Research and Development Program of China(2016YFD0100203 and 2016YFD0102000)the Key Scientific and Technological Projects of the Eighth Division of the Xinjiang Production and Construction Corps(XPCC),China(2024NY01,2023 NY09,2023 NY10)+2 种基金the Key Scientific and Technological Project of XPCC,China(2021AB010)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX22_0748)supported by the High-performance Computing Platform of the Bioinformatics Center,Nanjing Agricultural University,China。
文摘Gossypium raimondii(2n=2x=26,D_(5)),an untapped wild species,is the putative progenitor of the D-subgenome of G.hirsutum(2n=4x=52,AD_(1)),an extensively cultivated species.Here,we developed a G.hirsutum(recipient)–G.raimondii(donor)introgression population to exploit the favorable QTLs/genes and mapped potential quantitative trait loci(QTLs)from wild cotton species.The introgression population consisted of 256 lines with an introgression rate of 52.33%for the G.raimondii genome.The introgression segment length range was 0.03–19.12 Mb,with an average of 1.22 Mb.The coverage of total introgression fragments from G.raimondii was 386.98 Mb.Further genome-wide association analysis(Q+K+MLM)and QTL mapping(RSTEP-LRT)identified 59 common QTLs,including 14 stable QTLs and six common QTL(co-QTL)clusters,and one hotspot of micronaire(MIC).The common QTLs for seed index all showed positive additive effects,while the common QTLs for boll weight all had negative additive effects,indicating that the linkage between seed index and boll weight could be broken.QTLs for lint percentage showed positive effects and could be beneficial for improving cotton yield.Most QTLs for fiber quality had negative additive effects,implying these QTLs were domesticated/improved in G.hirsutum.A few fiber quality QTLs showed positive additive effects,so they could be used to improve cotton fiber quality.The introgression lines developed could be useful for molecular marker-assisted breeding and mapping QTLs precisely for mining desirable genes from the wild species G.raimondii.Such genes can improve cultivated cotton in the future through a designbreeding approach.
基金supported by the grants from the National Natural Science Foundation of China(31872497 and 32072844)the National Key Research and Development Program of China(2021YFD1800200 and 2016YFD0500207)the Laboratory of Lingnan Modern Agriculture Project,China(NT2021007)。
文摘TypeⅠinterferon(IFN)-mediated innate immune responses represent the first line of host defense against viral infection.However,the molecular mechanisms by which avian infuenza virus(AIV)inhibits typeⅠIFN production in ducks are not well understood.Here,we frst found that the polymerase basic 2(PB2)protein of H5N1 subtype AIV inhibited the typeⅠIFN responses by targeting duck mitochondrial antiviral signaling protein(MAVS).We further demonstrated that H5N1-PB2 bound to theΔtransmembrane(ΔTM)domain of duck MAVS,and the polymerase basic 1(PB1)binding domain(PBD)and RNA binding nuclear import domain(RND)of H5N1-PB2 interacted with MAVS to inhibit typeⅠIFN expression in ducks.Collectively,our fndings contribute to understanding the molecular mechanism by which AIV proteins regulate the retinoic acid-inducible geneⅠ(RIG-Ⅰ)-like receptor(RLR)signaling pathway to evade host antiviral immune responses in ducks.
基金Supported by National Natural Science Foundation Cultivation Project of Lishui University(036/2024)Municipal-Level Project:Pathways for Establishing Low-carbon Pilot Counties(FGLS202210).
文摘[Objectives]Farmland ginseng cultivation,as a sustainable alternative to traditional forest-clearing ginseng planting,requires systematic evaluation of soil optimization strategies.This study aimed to quantify the linkage between soil improvement outcomes and ginseng(Panax ginseng)yield across five regions in Yanbian Korean Autonomous Prefecture.[Methods]Soil improvement trials were conducted using farmland soils,with forest soils as the baseline.Soil nutrient contents were measured via soil agrochemical analysis method using a continuous flow analyzer.Statistical approaches,including significance tests,correlation analysis,and regression analysis,were applied to identify key factors influencing yield.[Results]Ginseng yield exhibited a significant positive correlation with organic matter content and available phosphorus,but a negative correlation with electrical conductivity,ammonium nitrogen,and available potassium.Wangqing and Liucai regions achieved post-improvement yields equivalent to 94%and 88%of forest soil yields,respectively,demonstrating the highest soil similarity to forest ecosystems.[Conclusions]Region-specific soil improvement protocols in Wangqing and Liucai show high replicability and efficacy.These strategies can serve as benchmarks for sustainable farmland ginseng cultivation,minimizing ecological disruption while maintaining productivity.
基金This study was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA28020300)the National Natural Science Foundation of China(Grant No.42250410332)+2 种基金the Key Foreign Cooperation Program of the Bureau of International Cooperation of the Chinese Academy of Sciences(Grant No.177GJHZ2022020BS)the‘Double-First Class’Initiative Program for Foreign Talents of Southwest University(Grant No.cstc2021ycjh-bgzxm0002)the‘Prominent Scientist Program’of Chongqing Talents(Grant No.cstc2021ycjh-bgzxm0020)。
文摘Deforestation is one of the most serious environmental problems facing humankind.It continues to escalate rapidly across many regions of the world,thereby deteriorating the forest soil quality.This has prompted a large number of field-based studies aimed at understanding the impacts of deforestation on soil properties.However,the lack of comprehensive meta-analyses that utilized these studies has limited our deeper understanding of how different soil properties,including the soil organic carbon(SOC)pool,respond to deforestation.To address this critical knowledge gap,we conducted a meta-analysis of 144 studies to explore the impacts of deforestation on soil chemical,physical,and biological properties,with special emphasis on the long-term changes in SOC,such as concentrations,stocks,and sequestration.The results revealed that deforestation significantly decreased soil organic matter,electrical conductivity,and base saturation by 52%,50%,and 98%,respectively.While deforestation increased soil total nitrogen content and decreased available phosphorus content by 51%and 99%,respectively,it resulted in slight decreases in some chemical properties,including soil pH(1%)and base cations(1%–13%).Deforestation significantly increased bulk density by 27%and soil erosion by 47%,but significantly decreased soil aggregate stability by 39%and saturated hydraulic conductivity by 63%.Soil microbial biomass C and N concentrations and enzyme activities were significantly decreased as a consequence of deforestation.Soil biological properties were much more affected by deforestation than soil physical and chemical properties.Regarding the SOC,the land use conversion from forest to pasture significantly increased SOC concentrations,stocks,and sequestration rates(11%–13%),whereas the land use conversions from forest to both plantation and cropland significantly decreased SOC concentrations,stocks,and sequestration rates(10%–43%).This observed decline in SOC accumulations decreased with increasing years after deforestation.The SOC dynamics following deforestation were predominantly regulated by microbial biomass concentrations,dehydrogenase activity,soil erosion,saturated hydraulic conductivity,aggregate stability,as well as concentrations of total organic carbon,total nitrogen,total phosphorus and organic matter.The present meta-analytical study provides compelling evidence that deforestation can induce profound changes in soil characteristics,including soil C contents,and has significant implications for soil health sustainability and climate change mitigation.
基金Project supported by the Significant Science and Technology Projects of LongMen Laboratory in Henan Province(231100220100,231100221100)the Key Research and Development Program of Henan province(231111222200)+2 种基金the College Students Innovations Special Project(202410482008)the National Natural Science Foundation of China(62275105,12104163)Basic Scientific Research Operating Expenses of Henan Academy of Sciences(240607004)。
文摘We successfully prepared a series of rare-earth doped borosilicate glasses using the melt-quenching method,and carefully investigated the luminescent properties and the spectral modulation of Tb/Eucodoped borosilicate glasses under UV(200-400 nm)excitation.The results show that the prepared samples have the characteristics of broadband response,excellent transparency and tunable luminescence.By adjusting the excitation wavelength,the emissions of Tb^(3+),Eu^(2+)and Eu^(3+)ions are observed,which exhibit yellow-green,blue,red color and multi-color even white emissions,respectively.Moreover,the energy transfer between Tb^(3+)and Eu^(3+)ions in the codoped glasses is confirmed.Tb^(3+)absorbs a large number of solar-blind light,transfers to Eu^(3+)and results in intense visible emission in a wide waveband range.This makes the Tb/Eu co-doped glass a desirable candidate for solar-blind light detections.The photodetection system was built and shows a strong and stable response to the UV light of 210-400 nm.Due to broad detection range,high sensitivity and stability,our results offer strong implications for the development of photodetection device for diverse applications.
基金supported by Beijing Natural Science Foundation(L232101).
文摘Background:To comprehensively analyze the clinical characteristics of patients who underwent gastric endoscopic submucosal dissection(ESD)and explore the incidence and influencing factors of postoperative pain.Methods:The clinical data of patients who underwent gastric ESD at our center from 2009 to 2024 were retrospectively analyzed.Pain severity was assessed using a visual analogue scale,with a score≥4 defined as postoperative pain.Based on the presence or absence of postoperative pain,patients were divided into a pain group and a control group.Independent factors influencing postoperative pain were identified using multivariate logistic regression analysis.To control for confounding bias,patients in the case and control groups were matched by sex and lesion size,and the matched participants were further analyzed using a conditional logistic regression model.Results:In total,993 patients were analyzed.The incidence of postoperative pain was 9.1%(95%confidence interval[CI],7.3-11.1).In the univariate analysis,sex,operation duration,anesthesia method,intraoperative electrocoagulation,nasogastric tube placement,and postoperative vomiting were significantly associated with postoperative pain.Multivariate analysis identified eight independent factors:male sex(odds ratio[OR],0.61;95%CI,0.37-0.97;p=0.04),operation duration(OR,1.29;95%CI,1.03-1.63;p=0.02),protuberant lesions(OR,0.43;95%CI,0.26-0.71;p<0.01),antral lesions(OR,1.84;95%CI,1.10-3.05;p=0.01),intubation general anesthesia(OR,0.40;95%CI,0.22-0.72;p=0.002),intraoperative electrocoagulation(OR,0.32;95%CI,0.19-0.55;p<0.01),nasogastric tube placement(OR,2.005;95%CI,1.12-3.57;p=0.01),and postoperative vomiting(OR,3.24;95%CI,1.40-7.47;p=0.005).Conditional logistic regression analysis further identified diabetes mellitus(OR,2.50;95%CI,1.03-6.06;p=0.04).Conclusion:Female sex,diabetes mellitus,concave-type lesions,lesions in the gastric antrum,non-intubation general anesthesia,absence of intraoperative electrocoagulation,prolonged operation duration,nasogastric tube placement,and postoperative vomiting were independent factors associated with moderate to severe pain after gastric ESD.For patients at increased risk of postoperative pain,appropriate prophylactic and therapeutic measures during the perioperative period may effectively alleviate pain following gastric ESD.
文摘Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.
文摘<strong>Background:</strong> Oncolytic herpes simplex virus (oHSV) have been proved effective and safe to treat tumors. Glycoprotein D (gD) has been engineered for targeting cancer cells and de-targeting normal cells successfully, however, the effectiveness and safety of oHSVs still need to be improved. <strong>Method:</strong> Here we sequenced the DNA encoding gD of our recently isolated new strain HSV-1-LXMW and compared the gD amino acid sequence with the gDs of other 7 HSV-1 and 3 HSV-2 strains. <strong>Results:</strong> Phylogenetic analysis revealed that HSV-1-LXMW is evolutionarily close to HSV-1-Patton and -KOS strains. The gD amino acid sequence alignment identified 19 conserved and 8 variable regions. We further predicted 10 new motifs in HSV gD for the first time and identified motif differences in HSV-1 and HSV-2. We summarized the gD-engineered oHSVs and found that some of the newly identified gD motifs are actually functional. <strong>Conclusion:</strong> Our results shed light on HSV gD biology and provided new directions for future gD functional studies and engineering in order to make better oHSVs.
基金supported by the National Natural Science Foundation of China (31271712)the National Key Technologies R&D Program of China (2013BAD01B02-8)
文摘Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate(EMS). F2 and F2:3generations produced from crosses of M8008 × YN15(MY) and M8008 × SJZ54(MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height(PH), spike length(SL),fertile spikelet number per spike(FSS), grain width(GW), grain length(GL), GL/GW ratio(GLW), and thousand-grain weight(TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat(SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci(QTLs) forming QTL clusters(one in MY and two in MS) were detected. The QTL clusters coinciding with(MY population) or near(MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density(FFD), and TGW and to increases in GLW. The cluster in the wmc25–barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant(SN), and SL.
基金the National Key Research and Development Program of China(2020YFB1806800).
文摘With the large-scale commercial launch of fifth generation(5G)mobile network,the development of new services and applications catering to the year 2030,along with the deep convergence of information,communication,and data technologies(ICDT),and the lessons and experiences from 5G practice will drive the evolution of the next generation of mobile networks.This article surveys the history and driving forces of the evolution of the mobile network architecture and proposes a logical function architecture for sixth generation(6G)mobile network.The proposed 6G network architecture is termed SOLIDS(related to the following basic features:soft,on-demand fulfillment,lite,native intelligence,digital twin,and native security),which can support self-generation,self-healing,self-evolution,and self-immunity without human involvement and address the primary issues in the legacy 5G network(e.g.,high cost,high power consumption,and highly complicated operation and maintenance),significantly well.
文摘OBJECTIVE:To verify the effects of scraping therapy on the weightlifting ability by measuring the subjective sensation,and changes of biomarkers.METHODS:Five students,who have been trained for 3 years in a sport school in China were participated in this study.A course of scraping therapy was applied to intervene during the normal 7-week of weightlifting training programme.The ability of weightlifting,the scale of rating perceived exertion and serum biochemical markers were measured before and after the intervention.RESULTS:Scraping therapy caused a significant increase in weightlifting ability(P<0.05).The level of rating perceived exertion remained stable with the increase in the training volume.Immuno-globulinA was significantly increased(P<0.05),and creatine kinase and blood urea nitrogen were significantly decreased(P<0.05).No significant changes were observed in white blood cell,neutrophil,and testosterone.CONCLUSION:Scraping therapy may facilitate weightlifting ability mainly by decreasing weight sensation and improving serum biochemical parameters.
基金funded by the National Natural Science Foundation of China (Grant No.: 81803812)
文摘Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of bacteria.The discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial agents.For the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS systems.This review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.
基金supported by the National Postdoctoral Program(2020M672782)National Natural Science Foundation of China(No.U1663225)+2 种基金Changjiang Scholars and Innovative Research Team in University(No.IRT15R52)National 111 project from the Ministry of Science and Technologythe Ministry of Education of China and the National Key R&D Program of China(No.2016YFA0202602)。
文摘Lithium-selenium(Li-Se)battery has attracted growing attention.Nevertheless,its practical application is still impeded by the shuttle effect of the formed polyselenides.Herein,we report in-situ hydrothermal weaving the three-dimensional(3 D)highly conductive hierarchically interconnected nanoporous web by threading microporous metal organic framework MIL-68(Al)crystals onto multi-walled carbon nanotubes(MWCNTs).Such 3 D hierarchically nanoporous web(3 D MIL-68(Al)@MWCNTs web)with a very high surface area,a large amount of micropores,electrical conductivity and elasticity strongly traps the soluble polyselenides during the electrochemical reaction and significantly facilitates lithium ion diffusion and electron transportation.Molecular dynamic calculation confirmed the strong affinity of MIL-68(Al)for the adsorption of polyselenides,quite suitable for Li-Se battery.Their hexahedral channels(1.56 nm)are more efficient for the confinement of polyselenides and for the diffusion of electrolytes compared to their smaller triangular channels(0.63 nm).All these excellent characteristics of 3 D MIL-68(Al)@MWCNTs web with suitable confinement of a large amount of selenium and the conductive linkage between MIL-68(Al)host by MWCNTs result in a high capacity of 453 m Ah/g at 0.2 C with 99.5%coulombic efficiency after 200 cycles with significantly improved cycle stability and rate performance.The 3 D MIL-68(Al)@MWCNTs web presents a good performance in Li-Se battery in term of the specific capacity and cycling stability and also in terms of rate performance compared with all the metal-organic framework(MOF)based or MOF derived porous carbons used in Li-Se battery.
基金Supported by Huguo Temple Hospital of Traditional Chinese Medicine Affiliated to Beijing University of Traditional Chinese Medicine:2017-3
文摘Objective:To observe the clinical effect difference between abdominal acupuncture combined with megestrol acetate and megestrol acetate for advanced cancer-related anorexia.Methods:A total of 60 patients with advanced cancer-related anorexia were randomly divided into an abdominal acupuncture combined with oral megestrol acetate treatment group(group A)and oral megestrol acetate treatment group(group B),with 30 cases in each group.Both groups were given nutritional assessment,nutritional supplementation,improvement of constipation,correction of electrolyte disturbance and other general treatments.In addition,group B was given another 160 mg megestrol acetate dispersible tablet once a day.Group A was treated with abdominal acupuncture on the basis of group B treatment,5 times a week,and both the groups were treated for 2 weeks.The appetite visual analog scale(VAS),Karnofsky(KPS)score,and body weight of the two groups were observed before and after treatment,and the clinical efficacy was evaluated.Results:After treatment,the appetite VAS in the both groups were lower than those before treatment,the Karnofsky(KPS)scores were higher than those before treatment,and the differences were statistically significant(both P<0.05).The differences of appetite VAS and KPS score before and after treatment in group A were higher than those of group B,and the differences were statistically significant(both P<0.05).After treatment,the body weights of the two groups were higher than those before treatment,and the difference of group A before and after treatment was higher than that of group B,but the difference was not statistically significant(P>0.05).The effective rate of group A was 76.7%and that of group B was 50%,the difference was statistically significant(P<0.05).Conclusion:Abdominal acupuncture combined with oral megestrol acetate and oral megestrol acetate both can effectively improve the appetite and functional status of patients with advanced cancer-related anorexia.Abdominal acupuncture can improve the curative effect,which is better than that of western medication alone,providing a new idea for the treatment of advanced cancer-related anorexia in the future.
基金This work was supported by grants from the Science and Technology Research Program of Henan Province(22170124)the Medical Science and Technology Research Program of Henan Province(SBGJ2018038)+1 种基金the Science and Technology Program of Guangdong(2019A030317019)the Medical Scientific Research Foundation of Guangdong Province(A2019232).
文摘A splicing mutation in VPS4B can cause dentin dysplasia type I(DD-I),a hereditary autosomal-dominant disorder characterized by rootless teeth,the etiology of which is genetically heterogeneous.In our study,dental follicle cells(DFCs)were isolated and cultured from a patient with DD-I and compared with those from an age-matched,healthy control.In a previous study,this DD-I patient was confirmed to have a loss-of-function splicing mutation in VPS4B(IVS7+46C>G).The results from this study showed that the isolated DFCs were vimentin-positive and CK14-negative,indicating that the isolated cells were derived from the mesenchyme.DFCs harboring the VPS4B mutation had a significantly higher proliferation rate from day 3 to day 8 than control DFCs,indicating that VPS4B is involved in cell proliferation.The cells were then replenished with osteogenic medium to investigate how the VPS4B mutation affected osteogenic differentiation.Induction of osteogenesis,detected by alizarin red and alkaline phosphatase staining in vitro,was decreased in the DFCs from the DD-I patient compared to the control DFCs.Furthermore,we also found that the VPS4B mutation in the DD-I patient downregulated the expression of osteoblast-related genes,such as ALP,BSP,OCN,RUNX2,and their encoded proteins.These outcomes confirmed that the DD-I-associated VPS4B mutation could decrease the capacity of DFCs to differentiate during the mineralization process and may also impair physiological root formation and bone remodeling.This might provide valuable insights and implications for exploring the pathological mechanisms underlying DD-I root development.
