Nervonic acid(NA) is a long-chain monounsaturated fatty acid with significant potential for neural fiber repair.In this study,a mixed fatty acid methyl ester was synthesized as the raw material through saponification ...Nervonic acid(NA) is a long-chain monounsaturated fatty acid with significant potential for neural fiber repair.In this study,a mixed fatty acid methyl ester was synthesized as the raw material through saponification of Acer truncatum Bunge seed oil.Based on the differences in boiling points and relative volatilities of various components,a four-stage vacuum batch distillation process was employed to enrich the nervonic acid methyl ester(NAME).The effect of distillation process parameters on enrichment efficiency was investigated,including distillation temperature,operating pressure,and reflux ratio.The purity of NAME achieved as 91.20% under optimal conditions and the corresponding yield was 48.91%.To further increase the purity,a low-temperature crystallization process was adopted and a final purity of NAME was obtained as 97.56%.Simulation of the above four-stage batch distillation was conducted using Aspen Plus software,and a continuous distillation processes was further simulated to establish a theoretical basis for future industrial-scale production.The results of experiments and simulation demonstrate that the integrated process of vacuum distillation and low-temperature crystallization exhibits remarkable separation performances,providing robust guidance for the production of high-purity NA.展开更多
Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats...Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult.A new combined process of molecular distillation,urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials.First,according to the difference in the mean free path of fatty acids,molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18-C20 fatty acids of stearic,oleic,linoleic,and linolenic acids.The content of C16-C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes,in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%.Then,according to the difference in carbon chain length and saturation of fatty acid,the contents of C22-C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification(SPOMFs)ratio as 0.6.In addition,all saturated fatty acids were basically separated.Finally,according to the difference in the solubility of fatty acids in solvents,the C18-C20 unsaturated fatty acids of oleic,linoleic,and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization.The content of C18-C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization.The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization.The combined process of molecular distillation,urea inclusion and low temperature crystallization innovatively adopted an efficient,simple and easy-toindustrial solvent crystallization method to separate erucic and nervonic acids,obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.展开更多
基金supported by the National Natural Science Foundation of China(22125802,22108150,22338001)。
文摘Nervonic acid(NA) is a long-chain monounsaturated fatty acid with significant potential for neural fiber repair.In this study,a mixed fatty acid methyl ester was synthesized as the raw material through saponification of Acer truncatum Bunge seed oil.Based on the differences in boiling points and relative volatilities of various components,a four-stage vacuum batch distillation process was employed to enrich the nervonic acid methyl ester(NAME).The effect of distillation process parameters on enrichment efficiency was investigated,including distillation temperature,operating pressure,and reflux ratio.The purity of NAME achieved as 91.20% under optimal conditions and the corresponding yield was 48.91%.To further increase the purity,a low-temperature crystallization process was adopted and a final purity of NAME was obtained as 97.56%.Simulation of the above four-stage batch distillation was conducted using Aspen Plus software,and a continuous distillation processes was further simulated to establish a theoretical basis for future industrial-scale production.The results of experiments and simulation demonstrate that the integrated process of vacuum distillation and low-temperature crystallization exhibits remarkable separation performances,providing robust guidance for the production of high-purity NA.
基金supported by the National Natural Science Foundation of China(22125802 and 22078010)Beijing Natural Science Foundation(2222017)Big Science Project from BUCT(XK180301).
文摘Nervonic acid is the world’s first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value.The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult.A new combined process of molecular distillation,urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials.First,according to the difference in the mean free path of fatty acids,molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18-C20 fatty acids of stearic,oleic,linoleic,and linolenic acids.The content of C16-C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes,in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%.Then,according to the difference in carbon chain length and saturation of fatty acid,the contents of C22-C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification(SPOMFs)ratio as 0.6.In addition,all saturated fatty acids were basically separated.Finally,according to the difference in the solubility of fatty acids in solvents,the C18-C20 unsaturated fatty acids of oleic,linoleic,and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization.The content of C18-C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization.The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization.The combined process of molecular distillation,urea inclusion and low temperature crystallization innovatively adopted an efficient,simple and easy-toindustrial solvent crystallization method to separate erucic and nervonic acids,obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.
