Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in ...Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments.展开更多
The integration of accurate detection and immediate bactericidal in a single platform significantly enhances pathogen monitoring and control capabilities,but nanomaterial functional compatibility remains a major chall...The integration of accurate detection and immediate bactericidal in a single platform significantly enhances pathogen monitoring and control capabilities,but nanomaterial functional compatibility remains a major challenge.Here,we developed a multifunctional nanoplatform integrating multimode flexible detection and magnetic aggregation-induced sterilization by utilizing the synergistic effect of quinone-phenol grafting nanozymes loaded with Pt single atoms(Pt SA@Q-ACDs)and magnetic carbon dots(Fe_(3)O_(4)@DCDs).In Pt SA@Q-ACDs,Pt SA increased the electron transfer from the quinone-phenol system,and the hydroxyl and pyridine state N defects sequentially synergized to form a double anchor site,the energy barrier for reducing H_(2)O_(2) was reduced,constituting a"1+1>2"bicatalytic system,which improved signal output and sterilization efficiency.Fe_(3)O_(4)@DCD enriches target bacteria through magnetic induction,dramatically reduces background interference,and increases the sterilization efficiency to 100%while realizing the detection of pathogenic bacteria at the single-cell level.Complementing this,we designed an intelligent portable device capable of dynamically switching and interpreting multimodal signals,enabling adaptable field deployment.The multifunctional platform can realize early warning from food processing to the terminal,combined with high bactericidal efficiency,and can implement"closed-loop monitoring"for pathogenic bacteria in an all-round way,which has great potential.展开更多
Aspergillus flavus(A.flavus)and its metabolites,aflatoxins,are widespread in crops and foods,threatening public health.Using chemical fungicides to control A.flavus can pose safety hazards and cause environmental poll...Aspergillus flavus(A.flavus)and its metabolites,aflatoxins,are widespread in crops and foods,threatening public health.Using chemical fungicides to control A.flavus can pose safety hazards and cause environmental pollution.Therefore,there is an urgent need for safe and effective antifungal agents to control A.flavus.In this study,we isolated Aspergillus luchuensisYZ-1 cell-free supernatant(CFS),volatiles,and mycelial extracts to investigate their inhibitory effects on A.flavus.Results indicate that the CFS exhibited greater efficacy in impeding the growth and synthesis of aflatoxin B1(AFB1)in A.flavus.Transcriptomic analysis revealed a significant downregulation of lipid metabolism,biosynthesis of other secondary metabolites,and glycan metabolism in A.flavus treated with CFS.Approximately 94%of the genes in the aflatoxin synthesis pathway were significantly downregulated.Furthermore,the CFS treatment significantly increased reactive oxygen species(ROS)production in A.flavus,affected its energy metabolism,and upregulated methane metabolism and the pentose phosphate pathway.We then investigated the inhibitory mechanism of YZ-1 against A.flavus by biochemical analysis.It was further confirmed that CFS had destructive effects in the cell wall and cell membrane of A.flavus,as indicated by leakage of nucleic acids,proteins,alkaline phosphatase(AKP),as well as decreased chitin and ergosterol content.In summary,this study provides novel insights into the mechanisms of CFS against A.flavus,suggesting its potential as a biocontrol agent in food and agriculture.展开更多
基金the financial support of National Key Research and Development program, China (2017YFC1601202 and 2017YFC1601200)China Postdoctoral Science Foundation (2021M690854)+2 种基金National Natural Science Foundation of China (31671951 and 31972175)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (2017BT01S174)Fundamental Research Funds for the Central Universities, China (JZ2020HGQA0151)
文摘Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments.
基金supported by the National Key Research and Development Program,China(2022YFF1100702,2022YFF1100703)the National Natu-ral Science Foundation of China(32230084,32102104)+1 种基金the Guangdong Provincial Key Research and Development Project(2022B11l1040002)the Anhui Natural Science Foundation(2208085J11).
文摘The integration of accurate detection and immediate bactericidal in a single platform significantly enhances pathogen monitoring and control capabilities,but nanomaterial functional compatibility remains a major challenge.Here,we developed a multifunctional nanoplatform integrating multimode flexible detection and magnetic aggregation-induced sterilization by utilizing the synergistic effect of quinone-phenol grafting nanozymes loaded with Pt single atoms(Pt SA@Q-ACDs)and magnetic carbon dots(Fe_(3)O_(4)@DCDs).In Pt SA@Q-ACDs,Pt SA increased the electron transfer from the quinone-phenol system,and the hydroxyl and pyridine state N defects sequentially synergized to form a double anchor site,the energy barrier for reducing H_(2)O_(2) was reduced,constituting a"1+1>2"bicatalytic system,which improved signal output and sterilization efficiency.Fe_(3)O_(4)@DCD enriches target bacteria through magnetic induction,dramatically reduces background interference,and increases the sterilization efficiency to 100%while realizing the detection of pathogenic bacteria at the single-cell level.Complementing this,we designed an intelligent portable device capable of dynamically switching and interpreting multimodal signals,enabling adaptable field deployment.The multifunctional platform can realize early warning from food processing to the terminal,combined with high bactericidal efficiency,and can implement"closed-loop monitoring"for pathogenic bacteria in an all-round way,which has great potential.
基金financial support of National key research and Development program(2022YFF1100700)Guangdong Provincial Key Research and Development Project(2022B1111040002)+1 种基金Anhui Provincial Natural Science Foundation(2208085J11)the Fundamental Research Funds for the Central Universities(JZ2022HGTB0271).
文摘Aspergillus flavus(A.flavus)and its metabolites,aflatoxins,are widespread in crops and foods,threatening public health.Using chemical fungicides to control A.flavus can pose safety hazards and cause environmental pollution.Therefore,there is an urgent need for safe and effective antifungal agents to control A.flavus.In this study,we isolated Aspergillus luchuensisYZ-1 cell-free supernatant(CFS),volatiles,and mycelial extracts to investigate their inhibitory effects on A.flavus.Results indicate that the CFS exhibited greater efficacy in impeding the growth and synthesis of aflatoxin B1(AFB1)in A.flavus.Transcriptomic analysis revealed a significant downregulation of lipid metabolism,biosynthesis of other secondary metabolites,and glycan metabolism in A.flavus treated with CFS.Approximately 94%of the genes in the aflatoxin synthesis pathway were significantly downregulated.Furthermore,the CFS treatment significantly increased reactive oxygen species(ROS)production in A.flavus,affected its energy metabolism,and upregulated methane metabolism and the pentose phosphate pathway.We then investigated the inhibitory mechanism of YZ-1 against A.flavus by biochemical analysis.It was further confirmed that CFS had destructive effects in the cell wall and cell membrane of A.flavus,as indicated by leakage of nucleic acids,proteins,alkaline phosphatase(AKP),as well as decreased chitin and ergosterol content.In summary,this study provides novel insights into the mechanisms of CFS against A.flavus,suggesting its potential as a biocontrol agent in food and agriculture.
