The internal transcribed spacer(ITS)region(ITS1,ITS2 and 5.8S rDNA)of the nuclear ribosomal DNA(nrDNA)was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of ...The internal transcribed spacer(ITS)region(ITS1,ITS2 and 5.8S rDNA)of the nuclear ribosomal DNA(nrDNA)was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi.Around 800 bp-1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested.Taking isolate USA as outgroup,the phylogenetic tree was constructed by means of maximum parsimony analysis,and the genetic evolution among isolates was analyzed.The results showed that there is a great difference between the base constitution of ITS 1 and ITS2 among various isolates.The seventeen isolates are classified into three groups,and the isolates from the same region belong to the same group,which shows the variation in geography.展开更多
Soybean is a leguminous crop that provides oil and protein. Exploring the genomic signatures of soybean evolution is crucial for breeding varieties with improved adaptability to environmental extremes. We analyzed the...Soybean is a leguminous crop that provides oil and protein. Exploring the genomic signatures of soybean evolution is crucial for breeding varieties with improved adaptability to environmental extremes. We analyzed the genome sequences of 2,214 soybeans and proposed a soybean evolutionary route, i.e., the expansion of annual wild soybean(Glycine soja Sieb. & Zucc.) from southern China and its domestication in central China, followed by the expansion and local breeding selection of its landraces(G. max(L.) Merr.). We observed that the genetic introgression in soybean landraces was mostly derived from sympatric rather than allopatric wild populations during the geographic expansion. Soybean expansion and breeding were accompanied by the positive selection of flowering time genes, including GmSPA3c. Our study sheds light on the evolutionary history of soybean and provides valuable genetic resources for its future breeding.展开更多
基金supported by National Natural Science Fundation of China(No.30400285,30671317)Postdoctoral Grant from Ag-riculture Sciences Academy of Heilongjiang Province(No.LRB06-010)+4 种基金China Postdoctoral Grant,Item for Teachers from Heilongjiang University(No.140022)Young People’s Science Fund of Heilongjiang Province(No.QC06C012)973(No.2004CB117203-4)the Opening Fund of Key Opening Laboratory of Physiology and Ecology of Crop in Cold Terra of Agriculture Ministry"the Cloning and Mapping of cDNA Sequence from Related Gene Resistant to Phytophthora sojaeInternational Tech-nology Cooperation Item(No.2005DFA30340).
文摘The internal transcribed spacer(ITS)region(ITS1,ITS2 and 5.8S rDNA)of the nuclear ribosomal DNA(nrDNA)was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi.Around 800 bp-1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested.Taking isolate USA as outgroup,the phylogenetic tree was constructed by means of maximum parsimony analysis,and the genetic evolution among isolates was analyzed.The results showed that there is a great difference between the base constitution of ITS 1 and ITS2 among various isolates.The seventeen isolates are classified into three groups,and the isolates from the same region belong to the same group,which shows the variation in geography.
基金supported by the National Key R&D Program of China(2021YFD1201601,2016YFD0100201,2020YFE0202300)the National Natural Science Foundation of China(32072091)+2 种基金the Platform of National Crop Germplasm Resources of China(2016-004,2017-004,2018-004,2019-04,2020-05)the Crop Germplasm Resources Protection(2016NWB036-05,2017NWB036-05,2018NWB03605,2019NWB036-05)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences(CAASZDRW202109)。
文摘Soybean is a leguminous crop that provides oil and protein. Exploring the genomic signatures of soybean evolution is crucial for breeding varieties with improved adaptability to environmental extremes. We analyzed the genome sequences of 2,214 soybeans and proposed a soybean evolutionary route, i.e., the expansion of annual wild soybean(Glycine soja Sieb. & Zucc.) from southern China and its domestication in central China, followed by the expansion and local breeding selection of its landraces(G. max(L.) Merr.). We observed that the genetic introgression in soybean landraces was mostly derived from sympatric rather than allopatric wild populations during the geographic expansion. Soybean expansion and breeding were accompanied by the positive selection of flowering time genes, including GmSPA3c. Our study sheds light on the evolutionary history of soybean and provides valuable genetic resources for its future breeding.
