This study examined the effects of various levels of dietary nonphytate phosphorus on laying performance and the expression patterns of phosphorus metabolism related genes in Dwarf pink-shell Jaying hens. A total of 4...This study examined the effects of various levels of dietary nonphytate phosphorus on laying performance and the expression patterns of phosphorus metabolism related genes in Dwarf pink-shell Jaying hens. A total of 405 28-week-old Dwarf pink-shell laying hens were fed the same corn-soybean basal meals but containing 0.20%, 0.25%, 0.30%, 0.35% or 0.40% nonphytate phosphorus. The results showed that feed intake, egg production, and average egg weights were quadratically correlated with dietary nonphytate phosphorus content （P 〈 0.05）, and the highest egg production, feed intake and average egg weights were achieved when dietary nonphytate phosphorus was at 0.3% （P 〈 0.05）. mRNA expression of intestinal sodium phosphorus co-transporter linearly decreased when dietary nonphytate phosphorus increased, mRNA and protein expression of intestinal calbindin and vitamin D receptor correlated quadratically with dietary nonphytate phosphorus, and the highest expression was found when dietary available phosphorus was at 0.2,5% to 0.3%. In conclusion, the ideal phosphorus requirement for Dwarf pink-shell layer hens is estimated to be 0.3% in a corn-soybean diet. With this level of phosphorus supplementation, calbindin and vitamin D receptor reached their highest expression.展开更多
Optical gain and loss of microcavity greatly affect the quality of lasing,how to improve optical gain and decrease optical loss is of great significance for the preparation of laser.In this study,four types standard m...Optical gain and loss of microcavity greatly affect the quality of lasing,how to improve optical gain and decrease optical loss is of great significance for the preparation of laser.In this study,four types standard microdisks with different thicknesses of 2.2μm,1.9μm,1.7μm,and 1.45μm were fabricated by micromachining technology process to modulate optical gain and loss of microdisk lasing.The whispering gallery mode lasing in the ultraviolet range of Ga N microdisk devices was investigated for these devices in order to clarify the effect of microdisk thickness on device characteristics.The quality factor Q and lasing mode number for different thicknesses are calculated from the stimulated spectra.The lifetimes of the exciton combination properties of the devices were observed using time-resolved PL spectroscopy.The lasing modes are modulated,and the lifetime decreases,while the Q factor of the devices first increases and then decreases with decreasing thickness.All these results are induced by optical gain and loss competition.展开更多
An ecological optimization project(semi-closed reclamation project)was implemented to control the invasion of Spartina alterniflora,and optimize the habitat of the Chongming Dongtan wetland,in the Changjiang(Yangtze)R...An ecological optimization project(semi-closed reclamation project)was implemented to control the invasion of Spartina alterniflora,and optimize the habitat of the Chongming Dongtan wetland,in the Changjiang(Yangtze)River Estuary.After project implementation,a macrobenthic ecological survey was conducted in a natural tidal flat and a semi-closed reclamation restoration area within the Chongming Dongtan wetland from 2019 to 2020.Compared with historical data before reclamation,findings showed that the groups,numbers,and species diversity of the macrobenthos increased significantly,and the ecological optimization project resulted in good ecological benefits.In addition,compared to the natural tidal flat,the number of collected macrobenthic phyla,and the macrobenthic density and biomass were significantly lower in the restoration area.Furthermore,the biodiversity index and functional redundancy of natural tidal flats were generally higher,indicating that the community composition and function of natural tidal flats were relatively more stable.Even though the species composition differed between a number of restoration areas and natural tidal flats,there was no difference in functional diversity,indicating that the effect of restoring ecological functions in restoration areas was optimal.Among them,the biodiversity and functional redundancy of Site S2 were significantly reduced,and the ecosystem function was extremely unstable.Habitat heterogeneity,vegetation community and decreasing salinity were the main factors that affected the ecological functions of macrobenthos.The ecological quality was also evaluated;the Transects N3 and N4 showed good quality.The overall ecological quality of the restoration area was generally high,but that of Site S2 was poor and that of Site S2E was merely good,which was mainly due to modifications of the ecological function of macrobenthos.It is suggested that reeds mowing and freshwater species release should be adopted in restoration areas to improve the community function and the environmental disturbance resistance of the macrobenthos.展开更多
Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical...Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical multiple bacterial infections and monitor food microbial contamination in production sites in real-time.In this work,we developed a 4-mercaptophenylboronic acid gold nanoparticles(4-MPBA-AuNPs)-functionalized hydrogel microneedle(MPBA-H-MN)for bacteria detection in skin interstitial fluid.MPBA-H-MN could conveniently capture and enrich a variety of bacteria within 5 min.Surface enhanced Raman spectroscopy(SERS)detection was then performed and combined with machine learning technology to distinguish and identify a variety of bacteria.Overall,the capture efficiency of this method exceeded 50%.In the concentration range of 1×10_(7) to 1×10^(10) colony-forming units/mL(CFU/mL),the corresponding SERS intensity showed a certain linear relationship with the bacterial concentration.Using random forest(RF)-based machine learning,bacteria were effectively distinguished with an accuracy of 97.87%.In addition,the harmless disposal of used MNs by photothermal ablation was convenient,environmentally friendly,and inexpensive.This technique provided a potential method for rapid and real-time diagnosis of multiple clinical bacterial infections and for monitoring microbial contamination of food in production sites.展开更多
Acute liver failure is a life-threatening clinical syndrome with a high mortality rate. Currently, the research on Astragaloside IV in liver diseases primarily focuses on liver cancer, and there is limited understandi...Acute liver failure is a life-threatening clinical syndrome with a high mortality rate. Currently, the research on Astragaloside IV in liver diseases primarily focuses on liver cancer, and there is limited understanding of its mechanism in acute liver failure’s innate immunity. Therefore, this study aims to investigate the potential protective effect of Astragaloside IV on acute liver failure and its impact on innate immune cells. The study employed D-GalN/LPS-induced acute liver failure mouse models and employed various techniques such as a range of molecular and analytical techniques. The experimental results demonstrated that treatment with Astragaloside IV significantly reduced the inflammatory response, alleviated liver injury, and improved the survival rate of mice with acute liver failure induced by D-GalN/LPS. Further investigations revealed that AS-IV played a beneficial role by regulating the proportion of CD11b<sup>+</sup>Ly6C<sup>hi</sup> monocytes and the secretion of inflammatory cytokines and anti-inflammatory metabolites. These findings suggest that the pharmacological mechanism of AS-IV may involve targeted regulation of CD11b<sup>+</sup>Ly6C<sup>hi</sup> monocytes in both peripheral blood and liver. The implications of this study’s results are twofold. Firstly, they provide a basis for the clinical application of AS-IV in treating liver failure, offering potential therapeutic benefits. Secondly, they serve as a reference for further development of safer and more effective modified compounds.展开更多
Objective: To evaluate the role of prevention and control strategies for nosocomial infection in a tertiary teaching hospital during the sudden outbreak of Corona Virus Disease 2019 (COVID-19). Methods: The hospital i...Objective: To evaluate the role of prevention and control strategies for nosocomial infection in a tertiary teaching hospital during the sudden outbreak of Corona Virus Disease 2019 (COVID-19). Methods: The hospital initiated an emergency plan involving multi-departmental defense and control. It adopted a series of nosocomial infection prevention and control measures, including strengthening pre-examination and triage, optimizing the consultation process, improving the hospital’s architectural composition, implementing graded risk management, enhancing personal protection, and implementing staff training and supervision. Descriptive research was used to evaluate the short-term effects of these in-hospital prevention and control strategies. The analysis compared changes in related evaluation indicators between January 24, 2020 and February 12, 2020 (Chinese Lunar New Year’s Eve 2020 to lunar January 19) and the corresponding lunar period of the previous year. Results: Compared to the same period last year, the outpatient fever rate increased by 1.85-fold (P P Conclusion: The nosocomial infection prevention and control strategies implemented during this specific period improved the detection and control abilities for the COVID-19 source of infection and enhanced the compliance with measures. This likely contributed significantly to avoiding the occurrence of nosocomial infection.展开更多
The Changxing Formation of the Upper Permian in the Sichuan Basin remains a focal area for natural gas exploration.Previous research on this formation has predominantly focused on platform-margin reefs in northeastern...The Changxing Formation of the Upper Permian in the Sichuan Basin remains a focal area for natural gas exploration.Previous research on this formation has predominantly focused on platform-margin reefs in northeastern Sichuan Basin,while reservoirs within the platform have received limited attention.This study addresses this gap by systematically analyzing bioclastic shoal reservoirs in the Zhongjiang-Moxi region of central Sichuan Basin,using integrated methods including core observations,petrographic analysis,well log interpretation,and 3D seismic data.Results reveal that the study area primarily hosts porous bioclastic limestone reservoirs,characterized by storage spaces dominated by intragranular and interparticle dissolution pores.These reservoirs exhibit distinct facies-controlled attributes.Key controlling factors include:(1)syn-to penecontemporaneous karstification,(2)sea-level fluctuations governing reservoir thickness and stacking patterns,(3)micro-paleogeomorphic variations influencing early exposure dissolution,and(4)dolomitization enhancing reservoir quality.Based on these findings,two depositional evolution models for intraplatform reservoirs of the Changxing Formation are proposed.展开更多
Objective:To quantitatively analyze global research trends on the gut microbiota and cardiovascular disease(CVD)from 2005–2025,identifying publication output,core themes,influential articles,and emerging research hot...Objective:To quantitatively analyze global research trends on the gut microbiota and cardiovascular disease(CVD)from 2005–2025,identifying publication output,core themes,influential articles,and emerging research hotspots.Methods:We retrieved publications related to gut microbiota and CVD from 2005–2025 using standard bibliographic databases.A bibliometric analysis was conducted to evaluate annual output growth,leading journals,country and institutional contributions,author collaborations,citation networks,and keyword co-occurrence patterns.Descriptive statistics and visualization tools were used to map the developmental trajectory and research hotspots of this field.Results:The analysis included a sharp rise in publications over the past two decades,with especially rapid growth after 2015.Collaborative networks highlighted a few key countries and research centers driving the field.Highly cited papers clustered around themes such as microbiota-derived metabolites such as trimethylamine N-oxide,inflammation,and the“gut-heart axis.”Keyword analysis indicated evolving focus from early descriptive studies to recent mechanistic and clinical translational research.Conclusion:Research linking gut microbiota and CVD has expanded exponentially in 2005–2025,transitioning from correlation studies to mechanistic and therapeutic explorations.The bibliometric trends underscore the gut-heart axis as an emerging interdisciplinary domain in cardiovascular research,with potential to inform novel preventive and therapeutic strategies.展开更多
N6-methyladenosine(m6A)modification,one of the most prevalent RNA epi-genetic modifications in eukaryotes,constitutes over 60%of all RNA methylation modifications.This dynamic modification regulates RNA processing,mat...N6-methyladenosine(m6A)modification,one of the most prevalent RNA epi-genetic modifications in eukaryotes,constitutes over 60%of all RNA methylation modifications.This dynamic modification regulates RNA processing,maturation,nucleocytoplasmic transport,translation efficiency,phase separation,and sta-bility,thereby linking its dysregulation to diverse physiological and pathological processes.METTL3,a core catalytic component of the methyltransferase complex responsible for m6A deposition,is frequently dysregulated in diseases,including colorectal cancer(CRC).Although METTL3’s involvement in CRC pathogenesis has been documented,its precise molecular mechanisms and functional roles remain incompletely understood.METTL3 mediates CRC progression-encompa-ssing proliferation,invasion,drug resistance,and metabolic reprogramming-through m6A-dependent modulation of both coding RNAs and noncoding RNAs.Its regulatory effects are primarily attributed to interactions with key signaling pathways at multiple stages of CRC development.Emerging evidence highlights METTL3 as a promising biomarker for CRC diagnosis and prognosis,as well as a potential therapeutic target.By synthesizing recent advances in METTL3 research within CRC,this review provides critical insights into novel strategies for clinical diagnosis and targeted therapy.展开更多
Achieving carbon peak and carbon neutrality goals has driven the development of the hydrogen energy industry,with water electrolysis being a key source of clean hydrogen.However,the slow kinetics of oxygen evolution r...Achieving carbon peak and carbon neutrality goals has driven the development of the hydrogen energy industry,with water electrolysis being a key source of clean hydrogen.However,the slow kinetics of oxygen evolution reaction(OER)at the anode,especially under high current densities,hinders industrial-scale water electrolysis for hydrogen production.The design and fabrication of non-precious metal electrocatalysts with high OER activity at high current densities remain challenging.In this study,we synthesized an Fe(OH)_(x)-NiCoP OER catalyst by electrodepositing amorphous Fe(OH)_(x) onto low-crystallinity phosphides,forming crystalline-amorphous interface nanoneedle arrays.Characterization results revealed that the crystallineamorphous interfaces shortened ion/electron diffusion paths during electrochemical reactions and protected phosphides from corrosion.The incorporation of Fe enhanced the material's conductivity,while the sea urchin-like clustered structure provided a high specific surface area and rough surface.Critically,the superhydrophilic nature of the catalyst facilitated rapid bubble detachment and enhanced electrolyte accessibility,which are pivotal for sustaining high-current-density operation.The Fe(OH)_(x)-NiCoP/NF catalyst exhibited excellent electrocatalytic activity for OER in alkaline solution,demonstrating an overpotential of 357 mV at an industrially relevant current density of 1000 mA cm^(-2) and sustained catalytic activity for 750 h.Surface reconstruction of Fe(OH)_(x)-NiCoP/NF during OER contributed to the improved electrochemical performance.This work underscores the synergistic role of superhydrophilicity and efficient gas release in achieving durable high-current OER performance.By integrating crystalline and amorphous active sites into a multi-component material system,this study offers a novel design strategy for efficient OER electrocatalysts under demanding industrial conditions.展开更多
The conversion of biomass into lactic acid is essential for meeting industrial demand sustainably,alleviating resource shortages and environmental issues with benefits such as controllable conditions,lower costs,and r...The conversion of biomass into lactic acid is essential for meeting industrial demand sustainably,alleviating resource shortages and environmental issues with benefits such as controllable conditions,lower costs,and recyclable catalysts over traditional methods.The one-pot conversion of biomass(e.g.,microalgae,lignocellulose)and platform compounds(e.g.,glucose,fructose,erythritol,glycerol)to lactic acid involves multiple reaction steps to generate precursors such as dihydroxyacetone and pyruvaldehyde.Mismatched reaction rates often cause significant side reactions,resulting in low lactic acid selectivity and massive byproducts such as formic acid,acetic acid,and acetolactate.Achieving high selectivity to lactic acid requires precise catalytic control to synchronize reaction rates,promoting the targeted formation of lactic acid.In this review,we describe the reaction pathways for lactic acid production from biomass and its platform compounds,summarizing the advancements in the selective synthesis of lactic acid using alkali/alkaline earth catalysts and Lewis acid catalysts which involve post-transition metal(Al,Ga,Sn,Pb,etc.),transition metal(Zr,Nb,Mo,etc.)and lanthanide catalysts,as well as redox catalysts(such as perovskite-type and photocatalytic catalysts).The methods for regulating the selectivity to biomass-derived lactic acid are also summarized.By reviewing the progress and challenges in catalyst development for biomass-based lactic acid,we provide an outlook on catalyst design and characterization,reaction mechanism studies,and practical applications.展开更多
BACKGROUND At present,the incidence of diabetic nephropathy is increasing year by year,and there are many studies on the pathogenesis of diabetic nephropathy,but it is still not completely clear.The final pathological...BACKGROUND At present,the incidence of diabetic nephropathy is increasing year by year,and there are many studies on the pathogenesis of diabetic nephropathy,but it is still not completely clear.The final pathological result of diabetic nephropathy is mainly glomerular cell fibrosis,and the roles of micro-RNA(miRNA)-29 and DNA methyl transferase(DNMTs)in cell fibrosis have been confirmed in other studies,but there is a lack of relevant research in the kidney at present.AIM To study the potential involvement of miRNA-29a-3p in fibrosis related to diabetic kidney disease(DKD).METHODS The expression of miR-29a-3p,DNMT3A/3B,fibrosis-related molecules,Wnt3a,β-catenin,Janus kinase 2,and signal transducer and activator of transcription 3 was assessed in SV40MES13 cells and diabetic mice using quantitative real-time PCR and western blotting.Furthermore,the expression changes of fibrosis-related molecules were further analyzed using immunofluorescence and immunohistochemical blotting.The renal pathological changes of DKD in each group were also studied using hematoxylin-eosin and periodate-Schiff reaction staining.RESULTS In both the in vivo and in vitro experiments,it was observed that high glucose induction significantly decreased miR-29a-3p expression.As a result of this downregulation,DKD-related fibrosis was found to be promoted,as confirmed by elevated expression levels ofα-smooth muscle actin,collagen type I,and fibronectin.MiR-29a-3p targets the 3’non-coding regions of DNMT3A and DNMT3B and inhibits their expression.Inhibition of DNMT3A and DNMT3B can reverse the effect of miR-29a-3p downregulation on DKD-related fibrosis.CONCLUSION MiR-29a-3p can regulate Wnt/β-catenin and Janus kinase/signal transducer and activator of transcription signal pathways by regulating and inhibiting the expression of DNMT3A/3B and thus participate in the inhibition of DKD-related fibrosis.展开更多
Background:Episodic memory loss is a prominent clinical manifestation of Alzheimer’s disease(AD),which is closely related to tau pathology and hippocampal impairment.Due to the heterogeneity of brain neurons,the spec...Background:Episodic memory loss is a prominent clinical manifestation of Alzheimer’s disease(AD),which is closely related to tau pathology and hippocampal impairment.Due to the heterogeneity of brain neurons,the specific roles of different brain neurons in terms of their sensitivity to tau accumulation and their contribution to AD-like social memory loss remain unclear.Therefore,further investigation is necessary.Methods:We investigated the effects of AD-like tau pathology by Tandem mass tag proteomic and phosphoproteomic analysis,social behavioural tests,hippocampal electrophysiology,immunofluorescence staining and in vivo optical fibre recording of GCaMP6f and iGABASnFR.Additionally,we utilized optogenetics and administered ursolic acid(UA)via oral gavage to examine the effects of these agents on social memory in mice.Results:The results of proteomic and phosphoproteomic analyses revealed the characteristics of ventral hippocampal CA1(vCA1)under both physiological conditions and AD-like tau pathology.As tau progressively accumulated,vCA1,especially its excitatory and parvalbumin(PV)neurons,were fully filled with mislocated and phosphorylated tau(p-Tau).This finding was not observed for dorsal hippocampal CA1(dCA1).The overexpression of human tau(hTau)in excitatory and PV neurons mimicked AD-like tau accumulation,significantly inhibited neuronal excitability and suppressed distinct discrimination-associated firings of these neurons within vCA1.Photoactivating excitatory and PV neurons in vCA1 at specific rhythms and time windows efficiently ameliorated tau-impaired social memory.Notably,1 month of UA administration efficiently decreased tau accumulation via autophagy in a transcription factor EB(TFEB)-dependent manner and restored the vCA1 microcircuit to ameliorate tau-impaired social memory.Conclusion:This study elucidated distinct protein and phosphoprotein networks between dCA1 and vCA1 and highlighted the susceptibility of the vCA1 microcircuit to AD-like tau accumulation.Notably,our novel findings regarding the efficacy of UA in reducing tau load and targeting the vCA1 microcircuit may provide a promising strategy for treating AD in the future.展开更多
BACKGROUND Continuous glucose monitoring(CGM)metrics,such as time in range(TIR)and glycemic risk index(GRI),have been linked to various diabetes-related complications,including diabetic foot(DF).AIM To investigate the...BACKGROUND Continuous glucose monitoring(CGM)metrics,such as time in range(TIR)and glycemic risk index(GRI),have been linked to various diabetes-related complications,including diabetic foot(DF).AIM To investigate the association between CGM-derived indicators and the risk of DF in individuals with type 2 diabetes mellitus(T2DM).METHODS A total of 591 individuals with T2DM(297 with DF and 294 without DF)were enrolled.Relevant clinical data,complications,comorbidities,hematological parameters,and 72-hour CGM data were collected.Logistic regression analysis was employed to examine the relationship between these measurements and the risk of DF.RESULTS Individuals with DF exhibited higher mean blood glucose(MBG)levels and increased proportions of time above range(TAR),TAR level 1,and TAR level 2,but lower TIR(all P<0.001).Patients with DF had significantly lower rates of achieving target ranges for TIR,TAR,and TAR level 2 than those without DF(all P<0.05).Logistic regression analysis revealed that GRI,MBG,and TAR level 1 were positively associated with DF risk,while TIR was inversely correlated(all P<0.05).Achieving TIR and TAR was inversely correlated with white blood cell count and glycated hemoglobin A1c levels(P<0.05).Additionally,achieving TAR was influenced by fasting plasma glucose,body mass index,diabetes duration,and antidiabetic medication use.CONCLUSION CGM metrics,particularly TIR and GRI,are significantly associated with the risk of DF in T2DM,emphasizing the importance of improved glucose control.展开更多
Objective:To investigate the preference characteristics and relative importance of each core factor in the teaching program for undergraduate nursing students during clinical practice,and to provide empirical support ...Objective:To investigate the preference characteristics and relative importance of each core factor in the teaching program for undergraduate nursing students during clinical practice,and to provide empirical support for the creation of a student-centered,formalized clinical teaching system that meets the actual needs of nursing students.Methods:The quantitative research method of discrete choice experiment was adopted,and the questionnaire was designed based on the random utility theory.Through a systematic literature review,semi-structured interviews,and two rounds of Delphi expert consultations,six core attributes of the instructor,namely educational qualifications,teaching methods,frequency of individualized guidance,operational practice opportunities,feedback timeliness,and instructor title,and their corresponding levels were determined.The study period was from January 2024 to January 2025,and 158 undergraduate nursing students who chose to intern at Deyang People’s Hospital were selected as the research subjects.A survey tool with 12 choice sets was created using Ngene software,and then statistical analysis was performed on the obtained data using the conditional Logit model to measure the impact of each attribute on the choice behavior of nursing students.Results:The results showed that the conditional Logit model fitted well(likelihood ratio chi-square=85.32,p<0.001).The analysis results indicated that the most important teaching attributes for undergraduate nursing students were,in order:the academic qualifications of the teaching instructor(master vs.Junior college,β=0.42,p<0.01),individualized guidance frequency(daily vs.Weekly,β=0.38,p<0.01),operational practice opportunities(more vs.less,β=0.31,p<0.05),and the timeliness of feedback(timely versus delayed,β=0.29,p<0.05).The influence of the title of the instructor was not statistically significant(p>0.05).Conclusion:Undergraduate nursing students show a clear and systematic preference structure for clinical teaching,with a high expectation of frequent personalized guidance from highly educated teachers,as well as sufficient operational opportunities and timely teaching feedback.展开更多
Upgrading of abundant cellulosic biomass to isosorbide can reduce the dependence on limited fossil resources and provide a sustainable way to produce isosorbide,utilized for polymers,medicine and health care product s...Upgrading of abundant cellulosic biomass to isosorbide can reduce the dependence on limited fossil resources and provide a sustainable way to produce isosorbide,utilized for polymers,medicine and health care product synth-esis.This review comprehensively examines the key steps and catalytic systems involved in the conversion of cel-lulose to isosorbide.Initially,the reaction pathway from cellulose to isosorbide is elucidated,emphasizing three critical steps:cellulose hydrolysis,glucose hydrogenation,and the two-step dehydration of sorbitol to produce isosorbide.Additionally,the activation energy and acidic sites during cellulose hydrolysis,the impact of metal particle size and catalyst support on hydrogenation,and the effects of catalyst acidity,pore structure,and reaction conditions on sorbitol dehydration have been thoroughly examined.Finally,the progress made in cellulose con-version to isosorbide is summarized,current challenges are highlighted,and future development trends are pro-jected in this review.展开更多
基金financially supported by the Chinese Universities Scientific Fund
文摘This study examined the effects of various levels of dietary nonphytate phosphorus on laying performance and the expression patterns of phosphorus metabolism related genes in Dwarf pink-shell Jaying hens. A total of 405 28-week-old Dwarf pink-shell laying hens were fed the same corn-soybean basal meals but containing 0.20%, 0.25%, 0.30%, 0.35% or 0.40% nonphytate phosphorus. The results showed that feed intake, egg production, and average egg weights were quadratically correlated with dietary nonphytate phosphorus content （P 〈 0.05）, and the highest egg production, feed intake and average egg weights were achieved when dietary nonphytate phosphorus was at 0.3% （P 〈 0.05）. mRNA expression of intestinal sodium phosphorus co-transporter linearly decreased when dietary nonphytate phosphorus increased, mRNA and protein expression of intestinal calbindin and vitamin D receptor correlated quadratically with dietary nonphytate phosphorus, and the highest expression was found when dietary available phosphorus was at 0.2,5% to 0.3%. In conclusion, the ideal phosphorus requirement for Dwarf pink-shell layer hens is estimated to be 0.3% in a corn-soybean diet. With this level of phosphorus supplementation, calbindin and vitamin D receptor reached their highest expression.
基金supported by State Key Laboratory of Luminescence and Applications(Grant No.SKLA-2021-04)the Foundation of Jiangsu Provincial Double-Innovation Doctor Program(Grant No.30644)+2 种基金the Natural Science Foundation of Jiangsu Province(Grant No.BK20215093)the Research Start-up Fund(Grant Nos.NY219147 and NY220181)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant Nos.SJCX210267 and SJCX220279)。
文摘Optical gain and loss of microcavity greatly affect the quality of lasing,how to improve optical gain and decrease optical loss is of great significance for the preparation of laser.In this study,four types standard microdisks with different thicknesses of 2.2μm,1.9μm,1.7μm,and 1.45μm were fabricated by micromachining technology process to modulate optical gain and loss of microdisk lasing.The whispering gallery mode lasing in the ultraviolet range of Ga N microdisk devices was investigated for these devices in order to clarify the effect of microdisk thickness on device characteristics.The quality factor Q and lasing mode number for different thicknesses are calculated from the stimulated spectra.The lifetimes of the exciton combination properties of the devices were observed using time-resolved PL spectroscopy.The lasing modes are modulated,and the lifetime decreases,while the Q factor of the devices first increases and then decreases with decreasing thickness.All these results are induced by optical gain and loss competition.
基金The Shanghai Chongming Dongtan Bird National Nature Reserve Resource Monitoring Project under contract No.CMDT-JC202101the Shanghai Municipal Science and Technology Commission under contract No.19ZR1436900。
文摘An ecological optimization project(semi-closed reclamation project)was implemented to control the invasion of Spartina alterniflora,and optimize the habitat of the Chongming Dongtan wetland,in the Changjiang(Yangtze)River Estuary.After project implementation,a macrobenthic ecological survey was conducted in a natural tidal flat and a semi-closed reclamation restoration area within the Chongming Dongtan wetland from 2019 to 2020.Compared with historical data before reclamation,findings showed that the groups,numbers,and species diversity of the macrobenthos increased significantly,and the ecological optimization project resulted in good ecological benefits.In addition,compared to the natural tidal flat,the number of collected macrobenthic phyla,and the macrobenthic density and biomass were significantly lower in the restoration area.Furthermore,the biodiversity index and functional redundancy of natural tidal flats were generally higher,indicating that the community composition and function of natural tidal flats were relatively more stable.Even though the species composition differed between a number of restoration areas and natural tidal flats,there was no difference in functional diversity,indicating that the effect of restoring ecological functions in restoration areas was optimal.Among them,the biodiversity and functional redundancy of Site S2 were significantly reduced,and the ecosystem function was extremely unstable.Habitat heterogeneity,vegetation community and decreasing salinity were the main factors that affected the ecological functions of macrobenthos.The ecological quality was also evaluated;the Transects N3 and N4 showed good quality.The overall ecological quality of the restoration area was generally high,but that of Site S2 was poor and that of Site S2E was merely good,which was mainly due to modifications of the ecological function of macrobenthos.It is suggested that reeds mowing and freshwater species release should be adopted in restoration areas to improve the community function and the environmental disturbance resistance of the macrobenthos.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82204340,82173954,and 82073815)the Natural Science Foundation of Jiangsu Province,China(Grant No.:BK20221048)+1 种基金the Jiangsu Funding Program for Excellent Postdoctoral Talent,China(Grant No.:2022ZB295)Key Laboratory Project of Quality Control of Chinese Herbal Medicines and Decoction Pieces,Gansu Institute for Drug Control,China(Grant No.:2024GSMPA-KL02).
文摘Bacterial infection is a major threat to global public health,and can cause serious diseases such as bacterial skin infection and foodborne diseases.It is essential to develop a new method to rapidly diagnose clinical multiple bacterial infections and monitor food microbial contamination in production sites in real-time.In this work,we developed a 4-mercaptophenylboronic acid gold nanoparticles(4-MPBA-AuNPs)-functionalized hydrogel microneedle(MPBA-H-MN)for bacteria detection in skin interstitial fluid.MPBA-H-MN could conveniently capture and enrich a variety of bacteria within 5 min.Surface enhanced Raman spectroscopy(SERS)detection was then performed and combined with machine learning technology to distinguish and identify a variety of bacteria.Overall,the capture efficiency of this method exceeded 50%.In the concentration range of 1×10_(7) to 1×10^(10) colony-forming units/mL(CFU/mL),the corresponding SERS intensity showed a certain linear relationship with the bacterial concentration.Using random forest(RF)-based machine learning,bacteria were effectively distinguished with an accuracy of 97.87%.In addition,the harmless disposal of used MNs by photothermal ablation was convenient,environmentally friendly,and inexpensive.This technique provided a potential method for rapid and real-time diagnosis of multiple clinical bacterial infections and for monitoring microbial contamination of food in production sites.
文摘Acute liver failure is a life-threatening clinical syndrome with a high mortality rate. Currently, the research on Astragaloside IV in liver diseases primarily focuses on liver cancer, and there is limited understanding of its mechanism in acute liver failure’s innate immunity. Therefore, this study aims to investigate the potential protective effect of Astragaloside IV on acute liver failure and its impact on innate immune cells. The study employed D-GalN/LPS-induced acute liver failure mouse models and employed various techniques such as a range of molecular and analytical techniques. The experimental results demonstrated that treatment with Astragaloside IV significantly reduced the inflammatory response, alleviated liver injury, and improved the survival rate of mice with acute liver failure induced by D-GalN/LPS. Further investigations revealed that AS-IV played a beneficial role by regulating the proportion of CD11b<sup>+</sup>Ly6C<sup>hi</sup> monocytes and the secretion of inflammatory cytokines and anti-inflammatory metabolites. These findings suggest that the pharmacological mechanism of AS-IV may involve targeted regulation of CD11b<sup>+</sup>Ly6C<sup>hi</sup> monocytes in both peripheral blood and liver. The implications of this study’s results are twofold. Firstly, they provide a basis for the clinical application of AS-IV in treating liver failure, offering potential therapeutic benefits. Secondly, they serve as a reference for further development of safer and more effective modified compounds.
文摘Objective: To evaluate the role of prevention and control strategies for nosocomial infection in a tertiary teaching hospital during the sudden outbreak of Corona Virus Disease 2019 (COVID-19). Methods: The hospital initiated an emergency plan involving multi-departmental defense and control. It adopted a series of nosocomial infection prevention and control measures, including strengthening pre-examination and triage, optimizing the consultation process, improving the hospital’s architectural composition, implementing graded risk management, enhancing personal protection, and implementing staff training and supervision. Descriptive research was used to evaluate the short-term effects of these in-hospital prevention and control strategies. The analysis compared changes in related evaluation indicators between January 24, 2020 and February 12, 2020 (Chinese Lunar New Year’s Eve 2020 to lunar January 19) and the corresponding lunar period of the previous year. Results: Compared to the same period last year, the outpatient fever rate increased by 1.85-fold (P P Conclusion: The nosocomial infection prevention and control strategies implemented during this specific period improved the detection and control abilities for the COVID-19 source of infection and enhanced the compliance with measures. This likely contributed significantly to avoiding the occurrence of nosocomial infection.
基金supported by the Innovation Consortium Science and Technology Cooperation Project jointly conducted by PetroChina and Southwest Petroleum University(No.2020CX010000).
文摘The Changxing Formation of the Upper Permian in the Sichuan Basin remains a focal area for natural gas exploration.Previous research on this formation has predominantly focused on platform-margin reefs in northeastern Sichuan Basin,while reservoirs within the platform have received limited attention.This study addresses this gap by systematically analyzing bioclastic shoal reservoirs in the Zhongjiang-Moxi region of central Sichuan Basin,using integrated methods including core observations,petrographic analysis,well log interpretation,and 3D seismic data.Results reveal that the study area primarily hosts porous bioclastic limestone reservoirs,characterized by storage spaces dominated by intragranular and interparticle dissolution pores.These reservoirs exhibit distinct facies-controlled attributes.Key controlling factors include:(1)syn-to penecontemporaneous karstification,(2)sea-level fluctuations governing reservoir thickness and stacking patterns,(3)micro-paleogeomorphic variations influencing early exposure dissolution,and(4)dolomitization enhancing reservoir quality.Based on these findings,two depositional evolution models for intraplatform reservoirs of the Changxing Formation are proposed.
基金Medical and Health Research Project of Yichang(Project No.:A23-1-006)。
文摘Objective:To quantitatively analyze global research trends on the gut microbiota and cardiovascular disease(CVD)from 2005–2025,identifying publication output,core themes,influential articles,and emerging research hotspots.Methods:We retrieved publications related to gut microbiota and CVD from 2005–2025 using standard bibliographic databases.A bibliometric analysis was conducted to evaluate annual output growth,leading journals,country and institutional contributions,author collaborations,citation networks,and keyword co-occurrence patterns.Descriptive statistics and visualization tools were used to map the developmental trajectory and research hotspots of this field.Results:The analysis included a sharp rise in publications over the past two decades,with especially rapid growth after 2015.Collaborative networks highlighted a few key countries and research centers driving the field.Highly cited papers clustered around themes such as microbiota-derived metabolites such as trimethylamine N-oxide,inflammation,and the“gut-heart axis.”Keyword analysis indicated evolving focus from early descriptive studies to recent mechanistic and clinical translational research.Conclusion:Research linking gut microbiota and CVD has expanded exponentially in 2005–2025,transitioning from correlation studies to mechanistic and therapeutic explorations.The bibliometric trends underscore the gut-heart axis as an emerging interdisciplinary domain in cardiovascular research,with potential to inform novel preventive and therapeutic strategies.
基金Supported by Jiangxi Provincial Natural Science Foundation,No.20242BAB25454the Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular of Ministry of Education of Gannan Medical University,No.XN202013+1 种基金Science and Technology Research Project of Jiangxi Provincial Department of Education,No.GJJ201528Startup Foundation for Advanced Talents of Gannan Medical University,No.QD202124.
文摘N6-methyladenosine(m6A)modification,one of the most prevalent RNA epi-genetic modifications in eukaryotes,constitutes over 60%of all RNA methylation modifications.This dynamic modification regulates RNA processing,maturation,nucleocytoplasmic transport,translation efficiency,phase separation,and sta-bility,thereby linking its dysregulation to diverse physiological and pathological processes.METTL3,a core catalytic component of the methyltransferase complex responsible for m6A deposition,is frequently dysregulated in diseases,including colorectal cancer(CRC).Although METTL3’s involvement in CRC pathogenesis has been documented,its precise molecular mechanisms and functional roles remain incompletely understood.METTL3 mediates CRC progression-encompa-ssing proliferation,invasion,drug resistance,and metabolic reprogramming-through m6A-dependent modulation of both coding RNAs and noncoding RNAs.Its regulatory effects are primarily attributed to interactions with key signaling pathways at multiple stages of CRC development.Emerging evidence highlights METTL3 as a promising biomarker for CRC diagnosis and prognosis,as well as a potential therapeutic target.By synthesizing recent advances in METTL3 research within CRC,this review provides critical insights into novel strategies for clinical diagnosis and targeted therapy.
基金the National Key Research and Development Program of China(2022YFB1105100)the National Natural Science Foundation of China(U2330105)the funding from Science Foundation of China University of Petroleum,Beijing(24620188JC005).
文摘Achieving carbon peak and carbon neutrality goals has driven the development of the hydrogen energy industry,with water electrolysis being a key source of clean hydrogen.However,the slow kinetics of oxygen evolution reaction(OER)at the anode,especially under high current densities,hinders industrial-scale water electrolysis for hydrogen production.The design and fabrication of non-precious metal electrocatalysts with high OER activity at high current densities remain challenging.In this study,we synthesized an Fe(OH)_(x)-NiCoP OER catalyst by electrodepositing amorphous Fe(OH)_(x) onto low-crystallinity phosphides,forming crystalline-amorphous interface nanoneedle arrays.Characterization results revealed that the crystallineamorphous interfaces shortened ion/electron diffusion paths during electrochemical reactions and protected phosphides from corrosion.The incorporation of Fe enhanced the material's conductivity,while the sea urchin-like clustered structure provided a high specific surface area and rough surface.Critically,the superhydrophilic nature of the catalyst facilitated rapid bubble detachment and enhanced electrolyte accessibility,which are pivotal for sustaining high-current-density operation.The Fe(OH)_(x)-NiCoP/NF catalyst exhibited excellent electrocatalytic activity for OER in alkaline solution,demonstrating an overpotential of 357 mV at an industrially relevant current density of 1000 mA cm^(-2) and sustained catalytic activity for 750 h.Surface reconstruction of Fe(OH)_(x)-NiCoP/NF during OER contributed to the improved electrochemical performance.This work underscores the synergistic role of superhydrophilicity and efficient gas release in achieving durable high-current OER performance.By integrating crystalline and amorphous active sites into a multi-component material system,this study offers a novel design strategy for efficient OER electrocatalysts under demanding industrial conditions.
基金supported by the National Key Research and Development Program of China(2022YFB1105100)the National Natural Science Foundation of China(U2330105)the funding from Science Foundation of China University of Petroleum,Beijing(24620188JC005).
文摘The conversion of biomass into lactic acid is essential for meeting industrial demand sustainably,alleviating resource shortages and environmental issues with benefits such as controllable conditions,lower costs,and recyclable catalysts over traditional methods.The one-pot conversion of biomass(e.g.,microalgae,lignocellulose)and platform compounds(e.g.,glucose,fructose,erythritol,glycerol)to lactic acid involves multiple reaction steps to generate precursors such as dihydroxyacetone and pyruvaldehyde.Mismatched reaction rates often cause significant side reactions,resulting in low lactic acid selectivity and massive byproducts such as formic acid,acetic acid,and acetolactate.Achieving high selectivity to lactic acid requires precise catalytic control to synchronize reaction rates,promoting the targeted formation of lactic acid.In this review,we describe the reaction pathways for lactic acid production from biomass and its platform compounds,summarizing the advancements in the selective synthesis of lactic acid using alkali/alkaline earth catalysts and Lewis acid catalysts which involve post-transition metal(Al,Ga,Sn,Pb,etc.),transition metal(Zr,Nb,Mo,etc.)and lanthanide catalysts,as well as redox catalysts(such as perovskite-type and photocatalytic catalysts).The methods for regulating the selectivity to biomass-derived lactic acid are also summarized.By reviewing the progress and challenges in catalyst development for biomass-based lactic acid,we provide an outlook on catalyst design and characterization,reaction mechanism studies,and practical applications.
基金Supported by the Chongqing Basic Research and Frontier Exploration Project,No.cstc2018jcyjAX0834.
文摘BACKGROUND At present,the incidence of diabetic nephropathy is increasing year by year,and there are many studies on the pathogenesis of diabetic nephropathy,but it is still not completely clear.The final pathological result of diabetic nephropathy is mainly glomerular cell fibrosis,and the roles of micro-RNA(miRNA)-29 and DNA methyl transferase(DNMTs)in cell fibrosis have been confirmed in other studies,but there is a lack of relevant research in the kidney at present.AIM To study the potential involvement of miRNA-29a-3p in fibrosis related to diabetic kidney disease(DKD).METHODS The expression of miR-29a-3p,DNMT3A/3B,fibrosis-related molecules,Wnt3a,β-catenin,Janus kinase 2,and signal transducer and activator of transcription 3 was assessed in SV40MES13 cells and diabetic mice using quantitative real-time PCR and western blotting.Furthermore,the expression changes of fibrosis-related molecules were further analyzed using immunofluorescence and immunohistochemical blotting.The renal pathological changes of DKD in each group were also studied using hematoxylin-eosin and periodate-Schiff reaction staining.RESULTS In both the in vivo and in vitro experiments,it was observed that high glucose induction significantly decreased miR-29a-3p expression.As a result of this downregulation,DKD-related fibrosis was found to be promoted,as confirmed by elevated expression levels ofα-smooth muscle actin,collagen type I,and fibronectin.MiR-29a-3p targets the 3’non-coding regions of DNMT3A and DNMT3B and inhibits their expression.Inhibition of DNMT3A and DNMT3B can reverse the effect of miR-29a-3p downregulation on DKD-related fibrosis.CONCLUSION MiR-29a-3p can regulate Wnt/β-catenin and Janus kinase/signal transducer and activator of transcription signal pathways by regulating and inhibiting the expression of DNMT3A/3B and thus participate in the inhibition of DKD-related fibrosis.
基金supported in part by the National Natural Science Foundation of China(91949205,82071219,82001134,31730035,81721005,and 82201584)the Hubei Provincial Key S&T Program(2018ACA142)the Guangdong Provincial Key S&T Program(2018B030336001).
文摘Background:Episodic memory loss is a prominent clinical manifestation of Alzheimer’s disease(AD),which is closely related to tau pathology and hippocampal impairment.Due to the heterogeneity of brain neurons,the specific roles of different brain neurons in terms of their sensitivity to tau accumulation and their contribution to AD-like social memory loss remain unclear.Therefore,further investigation is necessary.Methods:We investigated the effects of AD-like tau pathology by Tandem mass tag proteomic and phosphoproteomic analysis,social behavioural tests,hippocampal electrophysiology,immunofluorescence staining and in vivo optical fibre recording of GCaMP6f and iGABASnFR.Additionally,we utilized optogenetics and administered ursolic acid(UA)via oral gavage to examine the effects of these agents on social memory in mice.Results:The results of proteomic and phosphoproteomic analyses revealed the characteristics of ventral hippocampal CA1(vCA1)under both physiological conditions and AD-like tau pathology.As tau progressively accumulated,vCA1,especially its excitatory and parvalbumin(PV)neurons,were fully filled with mislocated and phosphorylated tau(p-Tau).This finding was not observed for dorsal hippocampal CA1(dCA1).The overexpression of human tau(hTau)in excitatory and PV neurons mimicked AD-like tau accumulation,significantly inhibited neuronal excitability and suppressed distinct discrimination-associated firings of these neurons within vCA1.Photoactivating excitatory and PV neurons in vCA1 at specific rhythms and time windows efficiently ameliorated tau-impaired social memory.Notably,1 month of UA administration efficiently decreased tau accumulation via autophagy in a transcription factor EB(TFEB)-dependent manner and restored the vCA1 microcircuit to ameliorate tau-impaired social memory.Conclusion:This study elucidated distinct protein and phosphoprotein networks between dCA1 and vCA1 and highlighted the susceptibility of the vCA1 microcircuit to AD-like tau accumulation.Notably,our novel findings regarding the efficacy of UA in reducing tau load and targeting the vCA1 microcircuit may provide a promising strategy for treating AD in the future.
基金Supported by Yunnan Province Academician(Expert)Workstation Project,No.202305AF150097the Basic Research Program of Yunnan Province(Kunming Medical University Joint Special Project),No.202101AY070001-276+3 种基金the National Natural Science Foundation of China,No.82160159the Key Project Program of Yunnan Province(Kunming Medical University Joint Special Project),No.202301AY070001-013the Major Science and Technology Project of Yunnan Province,No.202202AA100004the Double First-class University Construction Project of Yunnan University,No.CY22624106.
文摘BACKGROUND Continuous glucose monitoring(CGM)metrics,such as time in range(TIR)and glycemic risk index(GRI),have been linked to various diabetes-related complications,including diabetic foot(DF).AIM To investigate the association between CGM-derived indicators and the risk of DF in individuals with type 2 diabetes mellitus(T2DM).METHODS A total of 591 individuals with T2DM(297 with DF and 294 without DF)were enrolled.Relevant clinical data,complications,comorbidities,hematological parameters,and 72-hour CGM data were collected.Logistic regression analysis was employed to examine the relationship between these measurements and the risk of DF.RESULTS Individuals with DF exhibited higher mean blood glucose(MBG)levels and increased proportions of time above range(TAR),TAR level 1,and TAR level 2,but lower TIR(all P<0.001).Patients with DF had significantly lower rates of achieving target ranges for TIR,TAR,and TAR level 2 than those without DF(all P<0.05).Logistic regression analysis revealed that GRI,MBG,and TAR level 1 were positively associated with DF risk,while TIR was inversely correlated(all P<0.05).Achieving TIR and TAR was inversely correlated with white blood cell count and glycated hemoglobin A1c levels(P<0.05).Additionally,achieving TAR was influenced by fasting plasma glucose,body mass index,diabetes duration,and antidiabetic medication use.CONCLUSION CGM metrics,particularly TIR and GRI,are significantly associated with the risk of DF in T2DM,emphasizing the importance of improved glucose control.
基金Teaching Reform Research Project at Southwest Medical University(Project No.:JG2023jdyb034)Humanities and Social Sciences Research Project at Sichuan Vocational College of Nursing(Project No.:2022RWSY45)。
文摘Objective:To investigate the preference characteristics and relative importance of each core factor in the teaching program for undergraduate nursing students during clinical practice,and to provide empirical support for the creation of a student-centered,formalized clinical teaching system that meets the actual needs of nursing students.Methods:The quantitative research method of discrete choice experiment was adopted,and the questionnaire was designed based on the random utility theory.Through a systematic literature review,semi-structured interviews,and two rounds of Delphi expert consultations,six core attributes of the instructor,namely educational qualifications,teaching methods,frequency of individualized guidance,operational practice opportunities,feedback timeliness,and instructor title,and their corresponding levels were determined.The study period was from January 2024 to January 2025,and 158 undergraduate nursing students who chose to intern at Deyang People’s Hospital were selected as the research subjects.A survey tool with 12 choice sets was created using Ngene software,and then statistical analysis was performed on the obtained data using the conditional Logit model to measure the impact of each attribute on the choice behavior of nursing students.Results:The results showed that the conditional Logit model fitted well(likelihood ratio chi-square=85.32,p<0.001).The analysis results indicated that the most important teaching attributes for undergraduate nursing students were,in order:the academic qualifications of the teaching instructor(master vs.Junior college,β=0.42,p<0.01),individualized guidance frequency(daily vs.Weekly,β=0.38,p<0.01),operational practice opportunities(more vs.less,β=0.31,p<0.05),and the timeliness of feedback(timely versus delayed,β=0.29,p<0.05).The influence of the title of the instructor was not statistically significant(p>0.05).Conclusion:Undergraduate nursing students show a clear and systematic preference structure for clinical teaching,with a high expectation of frequent personalized guidance from highly educated teachers,as well as sufficient operational opportunities and timely teaching feedback.
文摘Upgrading of abundant cellulosic biomass to isosorbide can reduce the dependence on limited fossil resources and provide a sustainable way to produce isosorbide,utilized for polymers,medicine and health care product synth-esis.This review comprehensively examines the key steps and catalytic systems involved in the conversion of cel-lulose to isosorbide.Initially,the reaction pathway from cellulose to isosorbide is elucidated,emphasizing three critical steps:cellulose hydrolysis,glucose hydrogenation,and the two-step dehydration of sorbitol to produce isosorbide.Additionally,the activation energy and acidic sites during cellulose hydrolysis,the impact of metal particle size and catalyst support on hydrogenation,and the effects of catalyst acidity,pore structure,and reaction conditions on sorbitol dehydration have been thoroughly examined.Finally,the progress made in cellulose con-version to isosorbide is summarized,current challenges are highlighted,and future development trends are pro-jected in this review.
