Phase equilibrium modeling using internally consistent thermodynamic dataset and associated activity-composition(a-x)models are very helpful for quantifying P-T evolution for eclogite,which is the basis for decipherin...Phase equilibrium modeling using internally consistent thermodynamic dataset and associated activity-composition(a-x)models are very helpful for quantifying P-T evolution for eclogite,which is the basis for deciphering the geodynamic processes in subduction zones.In this study,we apply different versions of datasets(ds55 and ds62)and associated a-x relations to a wellestablished LT-HP eclogite at Huwan in the classic western Dabie orogen to constrain its P-T evolution.The eclogite comprises garnet+omphacite+amphibole+white mica+epidote+quartz+chlorite+rutile/ilmenite/sphene.Garnet porphyroblasts show mono-variation in the end members(spessartine from 17 mol%to 0,pyrope from 2 mol%to 18 mol%,almandine from 47 mol%to 64mol%and grossular from 35 mol%to 18 mol%)from core to rim.Phase diagrams combined with compositional isopleth thermobarometry show that dataset ds62 and associated a-x relations yield P_(max)of~33 kbar at~560℃,conflicting with our petrological observations and previous studies.On the other hand,phase equilibrium modeling using dataset ds62 and a revised symmetric garnet a-x model irrespective of Fe^(3+)(O)gives P_(max)of~27 kbar at~560℃,consistent with the results using dataset ds55 and associated a-x relations.Therefore,we recommend a symmetric model for garnet involving py,alm,gr and spss,without Fe~(3+)components,instead of the asymmetric garnet a-x relations involving py,alm,gr,spss and kho by White et al.(2014),for calculating phase diagrams for LT-(U)HP eclogite when using dataset ds62.In this study,the defined P-T path is characterized by a segment of the prograde evolution showing a first moderate slope,followed by gentle then steep slopes,representing the thermal structure evolution recorded by slab surface during continental subduction.Our work combined with previous studies conclude that in western Dabie,the Huwan HP eclogite belt to the north and the Hong'an HP eclogite belt to the south belong to the same HP slice overlying the Xinxian UHP slice.展开更多
Exploiting advanced nanocomposites isochronally integrating outstanding thermal conductivity(TC)and electromagnetic interference shielding effectiveness(EMI SE)can boost the cutting-edge application of phase change ma...Exploiting advanced nanocomposites isochronally integrating outstanding thermal conductivity(TC)and electromagnetic interference shielding effectiveness(EMI SE)can boost the cutting-edge application of phase change materials.Here,we report a tiramisu-like composite(GMP),where the typical“crust-and-cheese”hierarchical structure is replicated by an innovative two-step bidirectional freezing assembly(BFA)and compressive densification.Hierarchical-aligned graphene array(G-GA)with ultralow thermal resistance is fabricated through 1st BFA and graphitization.During the 2nd BFA,the MXene-CNF crosslinking network with hydrogen-bond actions is used for encapsulating polyethylene glycol(PEG)onto the microlayers of the G-GA skeleton.Remarkably,the microlaminated GMP4 achieves a recorded TC of 34.05 W m^(-1) K^(-1),unprecedented EMI SE of 87.4 dB,and preferable enthalpy density of 179.4 J cm^(-3),along with leakage-free function,and eminent thermal durability.Furthermore,the GMP-loaded equipment is demonstrated for efficient microelectronics cooling and sustainable solar energy utilization.This work opens new avenues for multiscale designing multifunctional macro-composites,broadening the application prospects in advanced electronics and solar energy utilization systems.展开更多
Dear Editor,Mutations in genomic sequences exhibit a strong correlation with various pathological processes of cancers[1].Currently,the next-generation sequencing technique[2]and polymerase chain reaction(PCR)were the...Dear Editor,Mutations in genomic sequences exhibit a strong correlation with various pathological processes of cancers[1].Currently,the next-generation sequencing technique[2]and polymerase chain reaction(PCR)were the established benchmarks for analyzing DNA mutations.However,the two methods necessitate intricate experimental preparation,costly instrumentation,and skilled personnel,making them challenging for rapid mutations analysis.More importantly,these methods lack adequate accuracy for one base mutations analysis[3].Therefore,the development of a reliable and exceptionally sensitive mutation analysis approach holds immense importance in cancer diagnosis and treatment.展开更多
Objective:To explore the application effect of bundle management in the safe nursing of patients with autoimmune encephalitis.Methods:Seventy-five patients with autoimmune encephalitis who met the inclusion criteria i...Objective:To explore the application effect of bundle management in the safe nursing of patients with autoimmune encephalitis.Methods:Seventy-five patients with autoimmune encephalitis who met the inclusion criteria in our hospital from January 2024 to June 2024 were randomly divided into two groups:an observation group of 39 patients and a control group of 36 patients.The control group received routine nursing care,while the observation group implemented a bundle management strategy based on routine nursing care.Safety nursing outcomes,clinical symptom improvement time,hospital stay,and neurologic function recovery were observed in both groups.Results:The incidence of adverse events in the observation group was 12.82%,significantly lower than the 33.33%in the control group,with a statistically significant difference(P<0.05).There was no statistically significant difference in restraint usage and ICU transfer rates between the two groups(P>0.05).The clinical symptom improvement time,hospital stay,and neurologic function recovery in the observation group were significantly better than those in the control group,with a statistically significant difference(P<0.05).Conclusion:Through the bundle management model,effective connections can be ensured in various aspects of treatment and rehabilitation for patients with autoimmune encephalitis,providing patients with comprehensive and multi-level nursing services and improving their overall satisfaction and treatment effectiveness.展开更多
In this paper,we constrain the symmetry energy at high densities in nuclear matter using recent observations of neutron stars based on the calculations of relativistic mean-field models.Using the observations of the n...In this paper,we constrain the symmetry energy at high densities in nuclear matter using recent observations of neutron stars based on the calculations of relativistic mean-field models.Using the observations of the neutron stars,we obtain the constraint on the symmetry energy at high densities,S(2_(ρ_(0)))=40.54±12.47 MeV,and S(3_(ρ_(0)))=44.12±29.38 MeV.展开更多
Objective:To explore the clinical effects of applying responsibility-based holistic nursing in elderly patients with severe pneumonia.Methods:Ninety-six elderly patients with severe pneumonia admitted to a hospital fr...Objective:To explore the clinical effects of applying responsibility-based holistic nursing in elderly patients with severe pneumonia.Methods:Ninety-six elderly patients with severe pneumonia admitted to a hospital from January 2023 to December 2024 were selected and divided into an observation group(52 cases)and a control group(44 cases)based on a random number table method.The observation group received responsibility-based holistic nursing,while the control group received basic nursing.The clinical effects(time to normalize body temperature,disappearance of cough,disappearance of dyspnea symptoms,disappearance of lung rales,changes in oxygenation index)and nursing satisfaction were observed in both groups.Results:The observation group showed superior results compared to the control group in terms of time to normalize body temperature,disappearance of cough,disappearance of dyspnea symptoms,disappearance of lung rales,and changes in oxygenation index,with statistically significant differences(P<0.05).The satisfaction rate with nursing services in the observation group(92.31%)was significantly higher than that in the control group(86.36%)(P<0.05).Conclusion:Implementing responsibility-based holistic nursing in elderly patients with severe pneumonia can improve their oxygenation function,enhance quality of life,and increase nursing satisfaction.展开更多
Objective Cachexia occurs in approximately half of hepatocellular carcinoma(HCC)patients as the disease progresses and is correlated with a poor prognosis.Therefore,early identification of HCC patients at risk of deve...Objective Cachexia occurs in approximately half of hepatocellular carcinoma(HCC)patients as the disease progresses and is correlated with a poor prognosis.Therefore,early identification of HCC patients at risk of developing cachexia and their prognosis is crucial.This study investigated the functional liver imaging score(FLIS)derived from gadoxetic acid-enhanced magnetic resonance imaging(MRI)to identify cachexia in HCC patients and their prognosis.Methods Pretreatment clinical and MRI data from 339 HCC patients who underwent gadoxetic acid-enhanced MRI scans were retrospectively collected.Patient weights were recorded for 6 months following the MRI scan to diagnose cachexia.The FLIS was calculated as the sum of the enhancement quality score,the excretion quality score,and the portal vein sign quality score.A Cox proportional hazards model was used to determine the significant factors affecting overall survival(OS).Multivariable logistic regression was then conducted to identify variables predicting cachexia in HCC patients,which were subsequently used to predict OS.Results Cox regression analysis revealed a significant association between cachexia and worse OS.Both FLIS(0–4 vs.5–6 points)(OR,9.20;95%CI:4.68–18.10;P<0.001)andα-fetoprotein>100 ng/mL(OR,4.08;95%CI:2.13–7.83;P<0.001)emerged as significant predictors of cachexia in patients with HCC.Furthermore,FLIS(0–4 vs.5–6 points)(HR,1.73;95%CI:1.19–2.51;P=0.004)was significantly associated with OS.Patients in the FLIS 0–4 points group had shorter OS than those in the FLIS 5–6 points group[20 months(95%CI,14.7–25.3)vs.43 months(95%CI,27.7–58.3);P=0.001].Conclusion Cachexia was associated with worse OS.The functional liver imaging score emerged as a significant predictor of cachexia in HCC patients and their prognosis.展开更多
BACKGROUND:There are currently no effective drugs to mitigate the ischemia/reperfusion injury caused by fluid resuscitation after hemorrhagic shock(HS).The aim of this study was to explore the potential of the histone...BACKGROUND:There are currently no effective drugs to mitigate the ischemia/reperfusion injury caused by fluid resuscitation after hemorrhagic shock(HS).The aim of this study was to explore the potential of the histone deacetylase 6(HDAC6)-specific inhibitor tubastatin A(TubA)to suppress nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation in macrophages under hypoxia/reoxygenation(H/R)conditions.METHODS:The viability of RAW264.7 cells subjected to H/R after treatment with different concentrations of TubA was assessed using a cell-counting kit-8(CCK8)assay.Briefly,2.5μmol/L TubA was used with RAW264.7 cells under H/R condition.RAW264.7 cells were divided into three groups,namely the control,H/R,and TubA groups.The levels of reactive oxygen species(ROS)in the cells were detected using fluorescence microscopy.The protein expression of HDAC6,heat shock protein 90(Hsp90),inducible nitric oxide synthase(iNOS),NLRP3,gasdermin-D(GSDMD),Caspase-1,GSDMD-N,and Caspase-1 p20 was detected by western blotting.The levels of interleukin-1β(IL-1β)and IL-18 in the supernatants were detected using enzyme-linked immunosorbent assay(ELISA).RESULTS:HDAC6,Hsp90,and iNOS expression levels were significantly higher(P<0.01)in the H/R group than in the control group,but lower in the TubA group than in the H/R group(P<0.05).When comparing the H/R group to the control group,ROS levels were significantly higher(P<0.01),but significantly reduced in the TubA group(P<0.05).The H/R group had higher NLRP3,GSDMD,Caspase-1,GSDMD-N,and Caspase-1 p20 expression levels than the control group(P<0.05),however,the TubA group had significantly lower expression levels than the H/R group(P<0.05).IL-1βand IL-18 levels in the supernatants were significantly higher in the H/R group compared to the control group(P<0.01),but significantly lower in the TubA group compared to the H/R group(P<0.01).CONCLUSION:TubA inhibited the expression of HDAC6,Hsp90,and iNOS in macrophages subjected to H/R.This inhibition led to a decrease in the content of ROS in cells,which subsequently inhibited the activation of the NLRP3 inflammasome and the secretion of IL-1βand IL-18.展开更多
Nasopharyngeal carcinoma(NPC)is a malignancy originating from the nasopharyngeal epithelial cells.Although some studies have identified potential biomarkers for early diagnosis of NPC based on metabolomics data(Chen e...Nasopharyngeal carcinoma(NPC)is a malignancy originating from the nasopharyngeal epithelial cells.Although some studies have identified potential biomarkers for early diagnosis of NPC based on metabolomics data(Chen et al.,2023;Zhou et al.,2023)and Epstein-Barr virus subtypes(Xu et al.,2019),their effectiveness and applicability need further investigation,particularly when consideringthe roles of oral microbiota.展开更多
To measure the carbon emission efficiency of China’s pharmaceutical manufacturing industry, explore the factors affecting the carbon emission efficiency of China’s pharmaceutical manufacturing industry, and provide ...To measure the carbon emission efficiency of China’s pharmaceutical manufacturing industry, explore the factors affecting the carbon emission efficiency of China’s pharmaceutical manufacturing industry, and provide reference for improving the carbon emission efficiency of China’s pharmaceutical manufacturing industry and promoting the government to formulate macro policies. Based on the data of the pharmaceutical manufacturing industry in 30 provinces of China from 2010 to 2019, and based on the SBM model and ML (Malmquist-Luenberger) index model, the carbon emission efficiency of the pharmaceutical manufacturing industry was calculated and its dynamic change was investigated, and the Tobit model was further used to explore the influencing factors of the carbon emission efficiency of the pharmaceutical manufacturing industry. The carbon emission efficiency of China’s inter-provincial pharmaceutical manufacturing industry has steadily improved. The carbon emission efficiency of the eastern region is higher than that of the western region, and that of the western region is higher than that of the central region. The eastern region is dominated by technological progress, and there is room for improvement in technological efficiency. The central and western regions are dominated by technological efficiency. Compared with technological efficiency, technological progress needs to be further improved. Environmental regulation, industrial agglomeration and technological innovation level positively affect carbon emission efficiency, while foreign investment level has no significant impact on carbon emission efficiency.展开更多
OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of ...OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG).METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/ dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that β-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of β-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats.展开更多
Hypobaric hypoxia can cause severe brain damage and mitochondrial dysfunction, and is involved in hypoxic brain injury. However, little is currently known about the mechanisms responsible for mi- tochondrial dysfuncti...Hypobaric hypoxia can cause severe brain damage and mitochondrial dysfunction, and is involved in hypoxic brain injury. However, little is currently known about the mechanisms responsible for mi- tochondrial dysfunction in hypobaric hypoxic brain damage. In this study, a rat model of hypobaric hypoxic brain injury was established to investigate the molecular mechanisms associated with mi- tochondrial dysfunction. As revealed by two-dimensional electrophoresis analysis, 16, 21, and 36 differential protein spots in cerebral mitochondria were observed at 6, 12, and 24 hours post-hypobaric hypoxia, respectively. Furthermore, ten protein spots selected from each hypobaric hypoxia subgroup were similarly regulated and were identified by mass spectrometry. These de- tected proteins included dihydropyrimidinase-related protein 2, creatine kinase B-type, is- ovaleryI-CoA dehydrogenase, elongation factor Ts, ATP synthase beta-subunit, 3-mercaptopyruvate sulfurtransferase, electron transfer flavoprotein alpha-subunit, Chain A of 2-enoyI-CoA hydratase, NADH dehydrogenase iron-sulfur protein 8 and tropomyosin beta chain. These ten proteins are all involved in the electron transport chain and the function of ATP synthase. Our findings indicate that hypobaric hypoxia can induce the differential expression of several cerebral mitochondrial proteins, which are involved in the regulation of mitochondrial energy production.展开更多
Two kinds of mesoporous carbon solid acids(LDMCE-SO3H and LDMCS-SO3H)were successfully prepared using masson pine alkali lignin as carbon source by evaporation-induced self-assembly(EISA)and salt-induced selfassembly(...Two kinds of mesoporous carbon solid acids(LDMCE-SO3H and LDMCS-SO3H)were successfully prepared using masson pine alkali lignin as carbon source by evaporation-induced self-assembly(EISA)and salt-induced selfassembly(SISA)followed by sulfonation,respectively.In terms of preparation process,SISA(self-assembly in water and preparation time of 2 days)is greener and simpler than EISA(self-assembly in ethanol and preparation time of 7 days).The prepared LDMCE-SO3H and LDMCS-SO3H exhibit obvious differences in structural characteristics such as pore channel structure,specific surface area,mesopore volume and the density of-SO3H groups.Furthermore,the catalytic performances of LDMCE-SO3H and LDMCS-SO3H were investigated in the hydrolysis of microcrystalline cellulose in water,and the glucose yields of 48.99%and 54.42%were obtained under the corresponding optimal reaction conditions.More importantly,the glucose yields still reached 28.85%and 30.35%after five runs,and restored to 39.02%and 45.98%through catalysts regeneration,respectively,demonstrating that LDMCE-SO3H and LDMCS-SO3H have excellent recyclability and regenerability.展开更多
AIM：To investigate the risk factor of primary open angle glaucoma（POAG）,which is the leading cause of irreversible blindness worldwide.An abnormally high level of endogenous formaldehyde（FA） has recently been fou...AIM：To investigate the risk factor of primary open angle glaucoma（POAG）,which is the leading cause of irreversible blindness worldwide.An abnormally high level of endogenous formaldehyde（FA） has recently been found correlated with cell death and neurodegenerative disease,raising the possibility of a putative correlation of abnormal endogenous FA with POAG.· METHODS：Thirty-four elderly patients with POAG and sixteen healthy controls were enrolled.Glaucomatous visual defects were present at both the functional（visual field） and structural[retinal nerve fiber layer（RNFL）thickness]levels.Morning urine samples were obtained and were analyzed by high-performance liquid chromatography（HPLC） to detect the endogenous FA level in a double blind manner.· RESULTS：Patients with POAG（P 〈0.05） had significantly higher urine FA levels.The urine FA level of patients with severe visual field defects[mean deviation（MD）≥12 dB]was significantly（P〈0.001） greater than that of patients with mild to moderate defects（MD〈12 dB）.By optical coherence tomography（OCT）,the superior and inferior RNFL thickness of POAG group was significantly（P〈0.001） thinner than in controls.Furthermore,the superior and inferior thinning of the RNFL was correlated with the elevation of urine FA concentration.CONCLUSION：Endogenous FA level is positively correlated with the neuronal defects of POAG.展开更多
AIMTo conduct a comparative study of effectiveness of silicone hydrogel contact lens and hydrogel contact lens, which are used in patients after laser-assisted subepithelial keratomileusis (LASEK).METHODSSixty-three p...AIMTo conduct a comparative study of effectiveness of silicone hydrogel contact lens and hydrogel contact lens, which are used in patients after laser-assisted subepithelial keratomileusis (LASEK).METHODSSixty-three patients (121 eyes) with a spherical equivalent ≤-5.0 D were chosen after undergoing LASEK in 2012 at Guangdong General Hospital. They were randomly divided into 2 groups. The silicone hydrogel group included 32 cases (61 eyes) that wore silicone hydrogel contact lenses for 4-6d after the operation, while the hydrogel group included 31 cases (60 eyes) who wore hydrogel contact lenses for 4-6d after the operation. Patients' self-reported postoperative symptoms (including pain, photophobia, tears, and foreign body sensation) were evaluated. The healing time of the corneal epithelium, the visual acuity of patients without contact lens after epithelial healing, and the incidence of delayed corneal epithelial shedding were also assessed. The follow-up time was 1mo.RESULTSPostoperative symptoms were milder in the silicone hydrogel group than in the hydrogel group. There were significant differences in pain, foreign body sensation, and photophobia between the 2 groups (P<0.05), although there was no significant difference in postoperative tearing (P>0.05). The healing time of the corneal epithelium in the silicone hydrogel lens group was markedly shorter than that in the hydrogel group (4.07±0.25 vs 4.33±0.82d, t=2.43, P=0.02). Visual acuity without contact lenses after healing of the corneal epithelium was better in the silicone hydrogel group compared with the hydrogel group (χ<sup>2</sup>=7.76, P=0.02). There was no significant difference in the occurrence of delayed corneal epithelial shedding between the 2 groups (P>0.05).CONCLUSIONPatients with LASEK using silicon hydrogel contact lenses had less discomfort and shorter corneal epithelial healing time compared with those using hydrogel contact lenses, suggesting that silicon hydrogel contact lenses may be considered to be a better choice of bandage contact lens after LASEK.展开更多
To explore the regulatory effects of jasmonic acid （JA） on the expression of the genes involved in capsaicin biosynthesis pathway, the mRNA expression of the genes was determined by fluorescence-based quantitative P...To explore the regulatory effects of jasmonic acid （JA） on the expression of the genes involved in capsaicin biosynthesis pathway, the mRNA expression of the genes was determined by fluorescence-based quantitative PCR 0, 4, 12, 24 and 48 h after chili peppers （Capsicum annuum var. conoides） were treated with 100 μmol/L JA, and the content of capsaicin was measured by high performance liquid chromatography （HPLC） 0, 1, 3, 6, 10 and 15 d after JA treatment. The results showed that JA upregulated the mRNA levels of pal, C4h, Comt, 4Cl, Hct, Paint, Bcat, FatA and pun1 in chili pepper, thus promoting the synthesis of capsaicin to different extents.展开更多
基金financially supported by the National Natural Science Foundation of China(No.41930215)the Fundamental Research Funds for the Central Universities,China University of Geosciences(Wuhan)。
文摘Phase equilibrium modeling using internally consistent thermodynamic dataset and associated activity-composition(a-x)models are very helpful for quantifying P-T evolution for eclogite,which is the basis for deciphering the geodynamic processes in subduction zones.In this study,we apply different versions of datasets(ds55 and ds62)and associated a-x relations to a wellestablished LT-HP eclogite at Huwan in the classic western Dabie orogen to constrain its P-T evolution.The eclogite comprises garnet+omphacite+amphibole+white mica+epidote+quartz+chlorite+rutile/ilmenite/sphene.Garnet porphyroblasts show mono-variation in the end members(spessartine from 17 mol%to 0,pyrope from 2 mol%to 18 mol%,almandine from 47 mol%to 64mol%and grossular from 35 mol%to 18 mol%)from core to rim.Phase diagrams combined with compositional isopleth thermobarometry show that dataset ds62 and associated a-x relations yield P_(max)of~33 kbar at~560℃,conflicting with our petrological observations and previous studies.On the other hand,phase equilibrium modeling using dataset ds62 and a revised symmetric garnet a-x model irrespective of Fe^(3+)(O)gives P_(max)of~27 kbar at~560℃,consistent with the results using dataset ds55 and associated a-x relations.Therefore,we recommend a symmetric model for garnet involving py,alm,gr and spss,without Fe~(3+)components,instead of the asymmetric garnet a-x relations involving py,alm,gr,spss and kho by White et al.(2014),for calculating phase diagrams for LT-(U)HP eclogite when using dataset ds62.In this study,the defined P-T path is characterized by a segment of the prograde evolution showing a first moderate slope,followed by gentle then steep slopes,representing the thermal structure evolution recorded by slab surface during continental subduction.Our work combined with previous studies conclude that in western Dabie,the Huwan HP eclogite belt to the north and the Hong'an HP eclogite belt to the south belong to the same HP slice overlying the Xinxian UHP slice.
基金support from the National Natural Science Foundation of China(No.21878218)the Tianjin Research Innovation Project for Postgraduate Students(No.2023KJ262)+2 种基金the State Grid Corporation of China’s Research Program(No.5419-202019385A)the Fundamental Research Funds for the Central Universities(No.92320006)the Tianjin Key Science and Technology Program(No.18ZXSZSF00030)。
文摘Exploiting advanced nanocomposites isochronally integrating outstanding thermal conductivity(TC)and electromagnetic interference shielding effectiveness(EMI SE)can boost the cutting-edge application of phase change materials.Here,we report a tiramisu-like composite(GMP),where the typical“crust-and-cheese”hierarchical structure is replicated by an innovative two-step bidirectional freezing assembly(BFA)and compressive densification.Hierarchical-aligned graphene array(G-GA)with ultralow thermal resistance is fabricated through 1st BFA and graphitization.During the 2nd BFA,the MXene-CNF crosslinking network with hydrogen-bond actions is used for encapsulating polyethylene glycol(PEG)onto the microlayers of the G-GA skeleton.Remarkably,the microlaminated GMP4 achieves a recorded TC of 34.05 W m^(-1) K^(-1),unprecedented EMI SE of 87.4 dB,and preferable enthalpy density of 179.4 J cm^(-3),along with leakage-free function,and eminent thermal durability.Furthermore,the GMP-loaded equipment is demonstrated for efficient microelectronics cooling and sustainable solar energy utilization.This work opens new avenues for multiscale designing multifunctional macro-composites,broadening the application prospects in advanced electronics and solar energy utilization systems.
文摘Dear Editor,Mutations in genomic sequences exhibit a strong correlation with various pathological processes of cancers[1].Currently,the next-generation sequencing technique[2]and polymerase chain reaction(PCR)were the established benchmarks for analyzing DNA mutations.However,the two methods necessitate intricate experimental preparation,costly instrumentation,and skilled personnel,making them challenging for rapid mutations analysis.More importantly,these methods lack adequate accuracy for one base mutations analysis[3].Therefore,the development of a reliable and exceptionally sensitive mutation analysis approach holds immense importance in cancer diagnosis and treatment.
文摘Objective:To explore the application effect of bundle management in the safe nursing of patients with autoimmune encephalitis.Methods:Seventy-five patients with autoimmune encephalitis who met the inclusion criteria in our hospital from January 2024 to June 2024 were randomly divided into two groups:an observation group of 39 patients and a control group of 36 patients.The control group received routine nursing care,while the observation group implemented a bundle management strategy based on routine nursing care.Safety nursing outcomes,clinical symptom improvement time,hospital stay,and neurologic function recovery were observed in both groups.Results:The incidence of adverse events in the observation group was 12.82%,significantly lower than the 33.33%in the control group,with a statistically significant difference(P<0.05).There was no statistically significant difference in restraint usage and ICU transfer rates between the two groups(P>0.05).The clinical symptom improvement time,hospital stay,and neurologic function recovery in the observation group were significantly better than those in the control group,with a statistically significant difference(P<0.05).Conclusion:Through the bundle management model,effective connections can be ensured in various aspects of treatment and rehabilitation for patients with autoimmune encephalitis,providing patients with comprehensive and multi-level nursing services and improving their overall satisfaction and treatment effectiveness.
基金supported by the National Natural Science Foundation of China(Nos.12275359,12375129,11875323,and 11961141003)the National Key R&D Program of China(No.2023YFA1606402)+2 种基金the Continuous Basic Scientific Research Project,the China Institute of Atomic Energy(Nos.YZ222407001301 and YZ232604001601)the Leading Innovation Project of the CNNC(Nos.LC192209000701 and LC202309000201)the computing server SCATP in China Institute of Atomic Energy and Basic Research Special Zone.
文摘In this paper,we constrain the symmetry energy at high densities in nuclear matter using recent observations of neutron stars based on the calculations of relativistic mean-field models.Using the observations of the neutron stars,we obtain the constraint on the symmetry energy at high densities,S(2_(ρ_(0)))=40.54±12.47 MeV,and S(3_(ρ_(0)))=44.12±29.38 MeV.
文摘Objective:To explore the clinical effects of applying responsibility-based holistic nursing in elderly patients with severe pneumonia.Methods:Ninety-six elderly patients with severe pneumonia admitted to a hospital from January 2023 to December 2024 were selected and divided into an observation group(52 cases)and a control group(44 cases)based on a random number table method.The observation group received responsibility-based holistic nursing,while the control group received basic nursing.The clinical effects(time to normalize body temperature,disappearance of cough,disappearance of dyspnea symptoms,disappearance of lung rales,changes in oxygenation index)and nursing satisfaction were observed in both groups.Results:The observation group showed superior results compared to the control group in terms of time to normalize body temperature,disappearance of cough,disappearance of dyspnea symptoms,disappearance of lung rales,and changes in oxygenation index,with statistically significant differences(P<0.05).The satisfaction rate with nursing services in the observation group(92.31%)was significantly higher than that in the control group(86.36%)(P<0.05).Conclusion:Implementing responsibility-based holistic nursing in elderly patients with severe pneumonia can improve their oxygenation function,enhance quality of life,and increase nursing satisfaction.
基金supported by grants from National Natural Science Foundation of China(No.82272064)Jiangsu Provincial Science and Technique Program(No.BK20221461)Zhongda Hospital Affiliated to Southeast University,Jiangsu Province High-level Hospital Paring Assistance Construction(No.zdlyg08).
文摘Objective Cachexia occurs in approximately half of hepatocellular carcinoma(HCC)patients as the disease progresses and is correlated with a poor prognosis.Therefore,early identification of HCC patients at risk of developing cachexia and their prognosis is crucial.This study investigated the functional liver imaging score(FLIS)derived from gadoxetic acid-enhanced magnetic resonance imaging(MRI)to identify cachexia in HCC patients and their prognosis.Methods Pretreatment clinical and MRI data from 339 HCC patients who underwent gadoxetic acid-enhanced MRI scans were retrospectively collected.Patient weights were recorded for 6 months following the MRI scan to diagnose cachexia.The FLIS was calculated as the sum of the enhancement quality score,the excretion quality score,and the portal vein sign quality score.A Cox proportional hazards model was used to determine the significant factors affecting overall survival(OS).Multivariable logistic regression was then conducted to identify variables predicting cachexia in HCC patients,which were subsequently used to predict OS.Results Cox regression analysis revealed a significant association between cachexia and worse OS.Both FLIS(0–4 vs.5–6 points)(OR,9.20;95%CI:4.68–18.10;P<0.001)andα-fetoprotein>100 ng/mL(OR,4.08;95%CI:2.13–7.83;P<0.001)emerged as significant predictors of cachexia in patients with HCC.Furthermore,FLIS(0–4 vs.5–6 points)(HR,1.73;95%CI:1.19–2.51;P=0.004)was significantly associated with OS.Patients in the FLIS 0–4 points group had shorter OS than those in the FLIS 5–6 points group[20 months(95%CI,14.7–25.3)vs.43 months(95%CI,27.7–58.3);P=0.001].Conclusion Cachexia was associated with worse OS.The functional liver imaging score emerged as a significant predictor of cachexia in HCC patients and their prognosis.
基金supported by National Natural Science Foundation of China(82102315).
文摘BACKGROUND:There are currently no effective drugs to mitigate the ischemia/reperfusion injury caused by fluid resuscitation after hemorrhagic shock(HS).The aim of this study was to explore the potential of the histone deacetylase 6(HDAC6)-specific inhibitor tubastatin A(TubA)to suppress nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation in macrophages under hypoxia/reoxygenation(H/R)conditions.METHODS:The viability of RAW264.7 cells subjected to H/R after treatment with different concentrations of TubA was assessed using a cell-counting kit-8(CCK8)assay.Briefly,2.5μmol/L TubA was used with RAW264.7 cells under H/R condition.RAW264.7 cells were divided into three groups,namely the control,H/R,and TubA groups.The levels of reactive oxygen species(ROS)in the cells were detected using fluorescence microscopy.The protein expression of HDAC6,heat shock protein 90(Hsp90),inducible nitric oxide synthase(iNOS),NLRP3,gasdermin-D(GSDMD),Caspase-1,GSDMD-N,and Caspase-1 p20 was detected by western blotting.The levels of interleukin-1β(IL-1β)and IL-18 in the supernatants were detected using enzyme-linked immunosorbent assay(ELISA).RESULTS:HDAC6,Hsp90,and iNOS expression levels were significantly higher(P<0.01)in the H/R group than in the control group,but lower in the TubA group than in the H/R group(P<0.05).When comparing the H/R group to the control group,ROS levels were significantly higher(P<0.01),but significantly reduced in the TubA group(P<0.05).The H/R group had higher NLRP3,GSDMD,Caspase-1,GSDMD-N,and Caspase-1 p20 expression levels than the control group(P<0.05),however,the TubA group had significantly lower expression levels than the H/R group(P<0.05).IL-1βand IL-18 levels in the supernatants were significantly higher in the H/R group compared to the control group(P<0.01),but significantly lower in the TubA group compared to the H/R group(P<0.01).CONCLUSION:TubA inhibited the expression of HDAC6,Hsp90,and iNOS in macrophages subjected to H/R.This inhibition led to a decrease in the content of ROS in cells,which subsequently inhibited the activation of the NLRP3 inflammasome and the secretion of IL-1βand IL-18.
基金supported by the National Key R&D Program of China(2022YFA1204000 to T.D.)the Guangdong Key Area Research and Development Program(2019B020228001 to T.D.)the National Natural Science Foundation of China(81971906 to T.D.).
文摘Nasopharyngeal carcinoma(NPC)is a malignancy originating from the nasopharyngeal epithelial cells.Although some studies have identified potential biomarkers for early diagnosis of NPC based on metabolomics data(Chen et al.,2023;Zhou et al.,2023)and Epstein-Barr virus subtypes(Xu et al.,2019),their effectiveness and applicability need further investigation,particularly when consideringthe roles of oral microbiota.
文摘To measure the carbon emission efficiency of China’s pharmaceutical manufacturing industry, explore the factors affecting the carbon emission efficiency of China’s pharmaceutical manufacturing industry, and provide reference for improving the carbon emission efficiency of China’s pharmaceutical manufacturing industry and promoting the government to formulate macro policies. Based on the data of the pharmaceutical manufacturing industry in 30 provinces of China from 2010 to 2019, and based on the SBM model and ML (Malmquist-Luenberger) index model, the carbon emission efficiency of the pharmaceutical manufacturing industry was calculated and its dynamic change was investigated, and the Tobit model was further used to explore the influencing factors of the carbon emission efficiency of the pharmaceutical manufacturing industry. The carbon emission efficiency of China’s inter-provincial pharmaceutical manufacturing industry has steadily improved. The carbon emission efficiency of the eastern region is higher than that of the western region, and that of the western region is higher than that of the central region. The eastern region is dominated by technological progress, and there is room for improvement in technological efficiency. The central and western regions are dominated by technological efficiency. Compared with technological efficiency, technological progress needs to be further improved. Environmental regulation, industrial agglomeration and technological innovation level positively affect carbon emission efficiency, while foreign investment level has no significant impact on carbon emission efficiency.
基金Supported by National Natural Science Foundation of China(30772794)National Science Foundation for Post-doctoral Scientists of China(2012M521412)Program for Innovative Research Team(in Science and Technology)in Henan College of Chinese Medicine(2011XCXTD01)
文摘OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG).METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/ dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that β-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of β-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats.
基金supported by the National Natural Science Foundation of China,No.81073152the Key Science Foundation of Tianjin in China,No.10JCZDJC21100+1 种基金the Natural Science Foundation of Tianjin in China,No.10JCYBJC14700,No.13JCQNJC13200the Science Foundation of Tianjin Key Laboratory in China,No.WHTD 201303-2
文摘Hypobaric hypoxia can cause severe brain damage and mitochondrial dysfunction, and is involved in hypoxic brain injury. However, little is currently known about the mechanisms responsible for mi- tochondrial dysfunction in hypobaric hypoxic brain damage. In this study, a rat model of hypobaric hypoxic brain injury was established to investigate the molecular mechanisms associated with mi- tochondrial dysfunction. As revealed by two-dimensional electrophoresis analysis, 16, 21, and 36 differential protein spots in cerebral mitochondria were observed at 6, 12, and 24 hours post-hypobaric hypoxia, respectively. Furthermore, ten protein spots selected from each hypobaric hypoxia subgroup were similarly regulated and were identified by mass spectrometry. These de- tected proteins included dihydropyrimidinase-related protein 2, creatine kinase B-type, is- ovaleryI-CoA dehydrogenase, elongation factor Ts, ATP synthase beta-subunit, 3-mercaptopyruvate sulfurtransferase, electron transfer flavoprotein alpha-subunit, Chain A of 2-enoyI-CoA hydratase, NADH dehydrogenase iron-sulfur protein 8 and tropomyosin beta chain. These ten proteins are all involved in the electron transport chain and the function of ATP synthase. Our findings indicate that hypobaric hypoxia can induce the differential expression of several cerebral mitochondrial proteins, which are involved in the regulation of mitochondrial energy production.
基金financial support from the National Natural Science Foundation of China(grant No.21706085)Subsidized Project for Postgraduates’Innovative Fund in Scientific Research of Huaqiao Universitythe Foundation of Key Laboratory of Pulp and Paper Science and Technology of Ministry of Education/Shandong Province of China(grant No.KF201804)。
文摘Two kinds of mesoporous carbon solid acids(LDMCE-SO3H and LDMCS-SO3H)were successfully prepared using masson pine alkali lignin as carbon source by evaporation-induced self-assembly(EISA)and salt-induced selfassembly(SISA)followed by sulfonation,respectively.In terms of preparation process,SISA(self-assembly in water and preparation time of 2 days)is greener and simpler than EISA(self-assembly in ethanol and preparation time of 7 days).The prepared LDMCE-SO3H and LDMCS-SO3H exhibit obvious differences in structural characteristics such as pore channel structure,specific surface area,mesopore volume and the density of-SO3H groups.Furthermore,the catalytic performances of LDMCE-SO3H and LDMCS-SO3H were investigated in the hydrolysis of microcrystalline cellulose in water,and the glucose yields of 48.99%and 54.42%were obtained under the corresponding optimal reaction conditions.More importantly,the glucose yields still reached 28.85%and 30.35%after five runs,and restored to 39.02%and 45.98%through catalysts regeneration,respectively,demonstrating that LDMCE-SO3H and LDMCS-SO3H have excellent recyclability and regenerability.
基金Supported by National Basic Research Program of China(973 Program,No.2012CB911004)the National Natural Science Foundation of China(No. 81041008No.30772376)
文摘AIM：To investigate the risk factor of primary open angle glaucoma（POAG）,which is the leading cause of irreversible blindness worldwide.An abnormally high level of endogenous formaldehyde（FA） has recently been found correlated with cell death and neurodegenerative disease,raising the possibility of a putative correlation of abnormal endogenous FA with POAG.· METHODS：Thirty-four elderly patients with POAG and sixteen healthy controls were enrolled.Glaucomatous visual defects were present at both the functional（visual field） and structural[retinal nerve fiber layer（RNFL）thickness]levels.Morning urine samples were obtained and were analyzed by high-performance liquid chromatography（HPLC） to detect the endogenous FA level in a double blind manner.· RESULTS：Patients with POAG（P 〈0.05） had significantly higher urine FA levels.The urine FA level of patients with severe visual field defects[mean deviation（MD）≥12 dB]was significantly（P〈0.001） greater than that of patients with mild to moderate defects（MD〈12 dB）.By optical coherence tomography（OCT）,the superior and inferior RNFL thickness of POAG group was significantly（P〈0.001） thinner than in controls.Furthermore,the superior and inferior thinning of the RNFL was correlated with the elevation of urine FA concentration.CONCLUSION：Endogenous FA level is positively correlated with the neuronal defects of POAG.
基金Supported by Science and Technology Projects of Guangdong Province (No.2013B021800178)Investigation on Health of Financial Staff in Guangzhou (No.Z012014075)
文摘AIMTo conduct a comparative study of effectiveness of silicone hydrogel contact lens and hydrogel contact lens, which are used in patients after laser-assisted subepithelial keratomileusis (LASEK).METHODSSixty-three patients (121 eyes) with a spherical equivalent ≤-5.0 D were chosen after undergoing LASEK in 2012 at Guangdong General Hospital. They were randomly divided into 2 groups. The silicone hydrogel group included 32 cases (61 eyes) that wore silicone hydrogel contact lenses for 4-6d after the operation, while the hydrogel group included 31 cases (60 eyes) who wore hydrogel contact lenses for 4-6d after the operation. Patients' self-reported postoperative symptoms (including pain, photophobia, tears, and foreign body sensation) were evaluated. The healing time of the corneal epithelium, the visual acuity of patients without contact lens after epithelial healing, and the incidence of delayed corneal epithelial shedding were also assessed. The follow-up time was 1mo.RESULTSPostoperative symptoms were milder in the silicone hydrogel group than in the hydrogel group. There were significant differences in pain, foreign body sensation, and photophobia between the 2 groups (P<0.05), although there was no significant difference in postoperative tearing (P>0.05). The healing time of the corneal epithelium in the silicone hydrogel lens group was markedly shorter than that in the hydrogel group (4.07±0.25 vs 4.33±0.82d, t=2.43, P=0.02). Visual acuity without contact lenses after healing of the corneal epithelium was better in the silicone hydrogel group compared with the hydrogel group (χ<sup>2</sup>=7.76, P=0.02). There was no significant difference in the occurrence of delayed corneal epithelial shedding between the 2 groups (P>0.05).CONCLUSIONPatients with LASEK using silicon hydrogel contact lenses had less discomfort and shorter corneal epithelial healing time compared with those using hydrogel contact lenses, suggesting that silicon hydrogel contact lenses may be considered to be a better choice of bandage contact lens after LASEK.
基金Supported by the Innovation Fund for Undergraduate of Jilin University(2016A82361)~~
文摘To explore the regulatory effects of jasmonic acid （JA） on the expression of the genes involved in capsaicin biosynthesis pathway, the mRNA expression of the genes was determined by fluorescence-based quantitative PCR 0, 4, 12, 24 and 48 h after chili peppers （Capsicum annuum var. conoides） were treated with 100 μmol/L JA, and the content of capsaicin was measured by high performance liquid chromatography （HPLC） 0, 1, 3, 6, 10 and 15 d after JA treatment. The results showed that JA upregulated the mRNA levels of pal, C4h, Comt, 4Cl, Hct, Paint, Bcat, FatA and pun1 in chili pepper, thus promoting the synthesis of capsaicin to different extents.
