Stroke is a leading cause of death worldwide. Up to one thousand potential drugs or interventions have been developed to treat stroke, out of which;60 have gone on to clinical trials. However, none of them has been su...Stroke is a leading cause of death worldwide. Up to one thousand potential drugs or interventions have been developed to treat stroke, out of which;60 have gone on to clinical trials. However, none of them has been successful. New insights into the molecular and cellular mechanisms of ischemia-induced injury are needed for discovering new therapeutic targets. Recently, Drosophila has been used to uncover new hypoxia-related genes. In this study, we describe an efficient and reliable assay with a sophisticated apparatus for studying the effects of oxygen deprivation on flies. Using this assay, wild-type flies were exposed to an anoxic environment for varying lengths of time, then the cumulative death rate and mobility recovery were systematically analyzed. We found that anoxia for over one hour caused lethality. The cumulative death rate on day 5 after anoxia was linearly and positively correlatedwith the duration of anoxia, and reached 50% when the duration was 2.5 h–3 h. We also found that the mobility recovery in normoxia was slow, as the climbing ability remained largely unchanged 4 h–6 h after 2.5-h of anoxia.We suggest that 2.5 h–3 h of anoxia and 4 h–6 h of recovery before mobility analysis are appropriate for future use of the anoxia assay.展开更多
Dear Editor,Diseases caused by ischemia,including coronary artery disease and stroke,are a leading cause of death worldwide[1].Restoration of the blood and oxygen supply after restriction also causes tissue damage by ...Dear Editor,Diseases caused by ischemia,including coronary artery disease and stroke,are a leading cause of death worldwide[1].Restoration of the blood and oxygen supply after restriction also causes tissue damage by activating a number of pathological pathways,such as inflammatory,oxidative stress,and cell death pathways that are mediated by microRNAs and hypoxia-inducible factors[1,2].展开更多
基金supported by the National Key Basic Research Program of China(2013CB530900)the National Natural Science Foundation of China(81371400 and 81771416)Shanghai Municipal Commission of Health and Family Planning(201740153)
文摘Stroke is a leading cause of death worldwide. Up to one thousand potential drugs or interventions have been developed to treat stroke, out of which;60 have gone on to clinical trials. However, none of them has been successful. New insights into the molecular and cellular mechanisms of ischemia-induced injury are needed for discovering new therapeutic targets. Recently, Drosophila has been used to uncover new hypoxia-related genes. In this study, we describe an efficient and reliable assay with a sophisticated apparatus for studying the effects of oxygen deprivation on flies. Using this assay, wild-type flies were exposed to an anoxic environment for varying lengths of time, then the cumulative death rate and mobility recovery were systematically analyzed. We found that anoxia for over one hour caused lethality. The cumulative death rate on day 5 after anoxia was linearly and positively correlatedwith the duration of anoxia, and reached 50% when the duration was 2.5 h–3 h. We also found that the mobility recovery in normoxia was slow, as the climbing ability remained largely unchanged 4 h–6 h after 2.5-h of anoxia.We suggest that 2.5 h–3 h of anoxia and 4 h–6 h of recovery before mobility analysis are appropriate for future use of the anoxia assay.
基金the National Natural Science Foundation of China(81771416,81650110527 and 8197100)Shanghai Municipal Commission of Health and Family Planning(201740153)Key Discipline of Chongming District,Shanghai,China,2018。
文摘Dear Editor,Diseases caused by ischemia,including coronary artery disease and stroke,are a leading cause of death worldwide[1].Restoration of the blood and oxygen supply after restriction also causes tissue damage by activating a number of pathological pathways,such as inflammatory,oxidative stress,and cell death pathways that are mediated by microRNAs and hypoxia-inducible factors[1,2].
