Photothermal synergistic catalytic systems for treating volatile organic compounds(VOCs)have attracted signif-icant attention due to their energy efficiency and potential to reduce carbon emissions.However,the mechani...Photothermal synergistic catalytic systems for treating volatile organic compounds(VOCs)have attracted signif-icant attention due to their energy efficiency and potential to reduce carbon emissions.However,the mechanism underlying the synergistic reaction remains a critical issue.This study introduces a photothermal synergistic system for the removal of ethyl acetate(EA)by synthesizing Cu-doped OMS-2(denoted as Cu-OMS-2).Under ultraviolet-visible(UV–Vis)irradiation in a flow system,the Cu-OMS-2 catalyst exhibited significantly enhanced performance in the EA degradation process,nearly doubling the effectiveness of pure OMS-2,and increasing carbon dioxide yield by 20%.This exceptional performance is attributed to the synergistic effect of increased oxygen vacancies(OV)at OMS-2 active sites and Cu doping,as confirmed by H2-TPR,O_(2)-TPD,and CO consump-tion measurements.This study clarifies the catalytic mechanism of light-assisted thermocatalysis and offers a novel strategy for designing photothermal catalysts with homogeneous Cu-doped nanorods for VOC removal.展开更多
Mycobacterium tuberculosis(Mtb)employs multiple mechanisms,such as phagocytosis and autophagy,to evade innate immune clearance and establish infection.In the present study,we identified the ESX-1 secretion-associated ...Mycobacterium tuberculosis(Mtb)employs multiple mechanisms,such as phagocytosis and autophagy,to evade innate immune clearance and establish infection.In the present study,we identified the ESX-1 secretion-associated protein EspL,which promotes Mtb survival by inhibiting phagosome maturation and autophagy initiation.EspL knockout decreased Mtb intracellular survival,while EspL overexpression increased bacterial survival by interfering with phagocytosis and autophagy.EspL interacts with ULK1 and promotes its phosphorylation at Ser^(757),leading to the inhibition of autophagy initiation.Additionally,overexpression of EspL reduced antigen presentation and T-cell responses both in vitro and in vivo.Our findings revealed that EspL interferes with autophagy and antigen presenta-tion by suppressing ULK1 activation.These insights provide a novel understanding of Mtb pathogenicity.展开更多
Novel carbon quantum dots（CQDs）/Bi OBr composite photocatalysts have been constructed through a facile hydrothermal synthesis in the presence of ionic liquid 1-hexadecyl-3-methylimidazolium bromide（[C16 mim]Br）. S...Novel carbon quantum dots（CQDs）/Bi OBr composite photocatalysts have been constructed through a facile hydrothermal synthesis in the presence of ionic liquid 1-hexadecyl-3-methylimidazolium bromide（[C16 mim]Br）. Series of characterizations have been performed to confirm the uniform distribution of CQDs in Bi OBr nanosheets and the synergistic effect for photocatalytic degradation organic pollutants between CQDs and Bi OBr. The results show that 3.1 wt% CQDs/Bi OBr photocatalyst possesses the best photocatalytic activity for the degradation of colorless antibiotic tetracycline（TC）, endocrine disrupter bisphenol A（BPA） and dye rhodamine B（Rh B）, under visible light irradiation, which exhibited the highest photocatalytic performance. The enhanced photocatalytic performance for CQDs/Bi OBr composites could be attributed to the wider optical absorption range and fast separation of photogenerated charge carriers after the introduction of CQDs. The key roles of CQDs for the enhanced photocatalytic activity of Bi OBr have been discussed. A possible mechanism of CQDs/Bi OBr on the enhancement of visible light performance was proposed.展开更多
Novel graphene-like boron nitride(BN)/Bi_(3)O_(4)Br photocatalysts have been controllably synthesized through a facile solvothermal method for the first time. Layer contact stacking between graphene-like BN and ultrat...Novel graphene-like boron nitride(BN)/Bi_(3)O_(4)Br photocatalysts have been controllably synthesized through a facile solvothermal method for the first time. Layer contact stacking between graphene-like BN and ultrathin Bi_(3)O_(4)Br was achieved with strong interaction. Dehalogenation is designed to harvest more visible light, and the ultrathin structure of Bi_(3)O_(4)Br is designed to accelerate charge transfer from inside to the surface. After graphene-like BN was engineered, photocatalytic performance greatly improved under visible light irradiation. Graphene-like BN can act as a surface electron-withdrawing center and adsorption center, facilitating molecular oxygen activation. O_(2)^(·-)was determined to be the main active species during the degradation process through analyses of electron spin resonance and XPS valence band spectra.展开更多
Non-radical activation of persulfate(PS)by photocatalysts is an effective approach for removing organic pollutants from aqueous environments.In this study,a novel Bi_(2)O_(3)/BiO_(1.3)I_(0.4)heterojunction was synthes...Non-radical activation of persulfate(PS)by photocatalysts is an effective approach for removing organic pollutants from aqueous environments.In this study,a novel Bi_(2)O_(3)/BiO_(1.3)I_(0.4)heterojunction was synthesized using a facile solvothermal approach and used for the first time for non-radical activation of PS to degrade propranolol(PRO)in the presence of visible light.The findings found that the degradation rate of PRO in the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)/PS system was significantly increased from 19%to more than 90%within 90 min compared to the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)system.This indicated that the composite system exerted an excellent synergistic effect between the photocatalyst and the persulfate-based oxygenation.Quenching tests and electron paramagnetic resonance demonstrated that the non-radical pathway with singlet oxygen as the active species played a major role in the photocatalytic process.The existence of photo-generated holes during the reaction could also be directly involved in the oxidation of pollutants.Meanwhile,a possible PRO degradation pathway was also proposed.Furthermore,the impacts of pH,humic acid and common anions on the PRO degradation by the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)/PS were explored,and the system’s stability and reusability were also studied.This study exhibits a highly productive catalyst for PS activation via a non-radical pathway and provides a new idea for the degradation of PRO.展开更多
Background:Diabetic foot ulcer(DFU)is one of the most common and complex complications of diabetes,but the underlying pathophysiology remains unclear.Single-cell RNA sequencing(scRNA-seq)has been conducted to explore ...Background:Diabetic foot ulcer(DFU)is one of the most common and complex complications of diabetes,but the underlying pathophysiology remains unclear.Single-cell RNA sequencing(scRNA-seq)has been conducted to explore novel cell types or molecular profiles of DFU from various perspectives.This study aimed to comprehensively analyze the potential mechanisms underlying impaired re-epithelization of DFU in a single-cell perspective.Methods:We conducted scRNA-seq on tissues from human normal skin,acute wound,and DFU to investigate the potential mechanisms underlying impaired epidermal differentiation and the pathological microenvironment.Pseudo-time and lineage inference analyses revealed the distinct states and transition trajectories of epidermal cells under different conditions.Transcription factor analysis revealed the potential regulatory mechanism of key subtypes of keratinocytes.Cell-cell interaction analysis revealed the regulatory network between the proinflammatory microenvironment and epidermal cells.Laser-capture microscopy coupled with RNA sequencing(LCM-seq)and multiplex immunohistochemistry were used to validate the expression and location of key subtypes of keratinocytes.Results:Our research provided a comprehensive map of the phenotypic and dynamic changes that occur during epidermal differentiation,alongside the corresponding regulatory networks in DFU.Importantly,we identified two subtypes of keratinocytes:basal cells(BC-2)and diabetes-associated keratinocytes(DAK)that might play crucial roles in the impairment of epidermal homeostasis.BC-2 and DAK showed a marked increase in DFU,with an inactive state and insufficient motivation for epidermal differentiation.BC-2 was involved in the cellular response and apoptosis processes,with high expression of TXNIP,IFITM1,and IL1R2.Additionally,the pro-differentiation transcription factors were downregulated in BC-2 in DFU,indicating that the differentiation process might be inhibited in BC-2 in DFU.DAK was associated with cellular glucose homeostasis.Furthermore,increased CCL2+CXCL2+fibroblasts,VWA1+vascular endothelial cells,and GZMA+CD8+T cells were detected in DFU.These changes in the wound microenvironment could regulate the fate of epidermal cells through the TNFSF12-TNFRSF12A,IFNG-IFNGR1/2,and IL-1B-IL1R2 pathways,which might result in persistent inflammation and impaired epidermal differentiation in DFU.Conclusions:Our findings offer novel insights into the pathophysiology of DFU and present potential therapeutic targets that could improve wound care and treatment outcomes for DFU patients.展开更多
The surficial inherent properties of TiO_(2)like exposed facet and crystalline state are vital for their surface reactions.However,efficiently controlling the specific crystal structure and the exposed crystal surface...The surficial inherent properties of TiO_(2)like exposed facet and crystalline state are vital for their surface reactions.However,efficiently controlling the specific crystal structure and the exposed crystal surface still faces big challenge.Here,the controlled solid phase transition of amorphous TiO_(2)to crystalline phase with exposed crystal facet(001)is achieved by photo-assisted atomic layer deposition(ALD)Pt process.Significantly,the obtained Pt/TiO_(2)film via photo-assisted treatment exhibits high sensing performance to NO and HF,and shows a lower optimized working temperature.The enhanced sensing performance is attributed to the metal-support strong interaction under oxidative atmosphere(O-SMSI).The facet effects leading to the unique distribution of charges at the interface combined with the catalytic effects result in the high sensing performance.This work provides a novel phase transition engineering strategy for regulating TiO_(2)from amorphous to crystalline phase,and the controllable synthesis of high Pt monatomic loading on TiO_(2)via ALD,which are critical for the accurate synthesis of efficient sensing and catalytic nanomaterials.展开更多
Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sens...Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.展开更多
基金supported by the Qilu University of Technology(Shandong Academy of Sciences),the Basic Research Project of Science,Education and Industry Integration Pilot Project(No.2022PY047).
文摘Photothermal synergistic catalytic systems for treating volatile organic compounds(VOCs)have attracted signif-icant attention due to their energy efficiency and potential to reduce carbon emissions.However,the mechanism underlying the synergistic reaction remains a critical issue.This study introduces a photothermal synergistic system for the removal of ethyl acetate(EA)by synthesizing Cu-doped OMS-2(denoted as Cu-OMS-2).Under ultraviolet-visible(UV–Vis)irradiation in a flow system,the Cu-OMS-2 catalyst exhibited significantly enhanced performance in the EA degradation process,nearly doubling the effectiveness of pure OMS-2,and increasing carbon dioxide yield by 20%.This exceptional performance is attributed to the synergistic effect of increased oxygen vacancies(OV)at OMS-2 active sites and Cu doping,as confirmed by H2-TPR,O_(2)-TPD,and CO consump-tion measurements.This study clarifies the catalytic mechanism of light-assisted thermocatalysis and offers a novel strategy for designing photothermal catalysts with homogeneous Cu-doped nanorods for VOC removal.
基金supported by the National Natural Science Foundation of China under grant number U21A20259the National Key Research and Development Program of China under grant number 2021YFD1800401.
文摘Mycobacterium tuberculosis(Mtb)employs multiple mechanisms,such as phagocytosis and autophagy,to evade innate immune clearance and establish infection.In the present study,we identified the ESX-1 secretion-associated protein EspL,which promotes Mtb survival by inhibiting phagosome maturation and autophagy initiation.EspL knockout decreased Mtb intracellular survival,while EspL overexpression increased bacterial survival by interfering with phagocytosis and autophagy.EspL interacts with ULK1 and promotes its phosphorylation at Ser^(757),leading to the inhibition of autophagy initiation.Additionally,overexpression of EspL reduced antigen presentation and T-cell responses both in vitro and in vivo.Our findings revealed that EspL interferes with autophagy and antigen presenta-tion by suppressing ULK1 activation.These insights provide a novel understanding of Mtb pathogenicity.
基金financially supported by the National Natural Science Foundation of China (Nos. 21471069, 21476098 and 21576123)Jiangsu University Scientific Research Funding (No. 11JDG0146)Doctoral Innovation Fund of Jiangsu Province (No. KYCX17_1791)
文摘Novel carbon quantum dots（CQDs）/Bi OBr composite photocatalysts have been constructed through a facile hydrothermal synthesis in the presence of ionic liquid 1-hexadecyl-3-methylimidazolium bromide（[C16 mim]Br）. Series of characterizations have been performed to confirm the uniform distribution of CQDs in Bi OBr nanosheets and the synergistic effect for photocatalytic degradation organic pollutants between CQDs and Bi OBr. The results show that 3.1 wt% CQDs/Bi OBr photocatalyst possesses the best photocatalytic activity for the degradation of colorless antibiotic tetracycline（TC）, endocrine disrupter bisphenol A（BPA） and dye rhodamine B（Rh B）, under visible light irradiation, which exhibited the highest photocatalytic performance. The enhanced photocatalytic performance for CQDs/Bi OBr composites could be attributed to the wider optical absorption range and fast separation of photogenerated charge carriers after the introduction of CQDs. The key roles of CQDs for the enhanced photocatalytic activity of Bi OBr have been discussed. A possible mechanism of CQDs/Bi OBr on the enhancement of visible light performance was proposed.
基金financially supported by the Fundamental Research Funds for the Central Universities (No.30922010302)the Start-Up Grant from Nanjing University of Science and Technology (AE89991/397)。
文摘Novel graphene-like boron nitride(BN)/Bi_(3)O_(4)Br photocatalysts have been controllably synthesized through a facile solvothermal method for the first time. Layer contact stacking between graphene-like BN and ultrathin Bi_(3)O_(4)Br was achieved with strong interaction. Dehalogenation is designed to harvest more visible light, and the ultrathin structure of Bi_(3)O_(4)Br is designed to accelerate charge transfer from inside to the surface. After graphene-like BN was engineered, photocatalytic performance greatly improved under visible light irradiation. Graphene-like BN can act as a surface electron-withdrawing center and adsorption center, facilitating molecular oxygen activation. O_(2)^(·-)was determined to be the main active species during the degradation process through analyses of electron spin resonance and XPS valence band spectra.
基金supported by the Basic Research Plan of Natural Science in Shaanxi Province-General Project(Nos.2023-JC-QN-0294,2023-JC-YB-094,2020JQ-740)the Postdoctoral Research Foundation of China(No.2020M673440)+1 种基金the National Natural Science Foundation of China(No.22303064)the Education Department Fund in Shaanxi Province(No.2021JK0763)。
文摘Non-radical activation of persulfate(PS)by photocatalysts is an effective approach for removing organic pollutants from aqueous environments.In this study,a novel Bi_(2)O_(3)/BiO_(1.3)I_(0.4)heterojunction was synthesized using a facile solvothermal approach and used for the first time for non-radical activation of PS to degrade propranolol(PRO)in the presence of visible light.The findings found that the degradation rate of PRO in the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)/PS system was significantly increased from 19%to more than 90%within 90 min compared to the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)system.This indicated that the composite system exerted an excellent synergistic effect between the photocatalyst and the persulfate-based oxygenation.Quenching tests and electron paramagnetic resonance demonstrated that the non-radical pathway with singlet oxygen as the active species played a major role in the photocatalytic process.The existence of photo-generated holes during the reaction could also be directly involved in the oxidation of pollutants.Meanwhile,a possible PRO degradation pathway was also proposed.Furthermore,the impacts of pH,humic acid and common anions on the PRO degradation by the Bi_(2)O_(3)/BiO_(1.3)I_(0.4)/PS were explored,and the system’s stability and reusability were also studied.This study exhibits a highly productive catalyst for PS activation via a non-radical pathway and provides a new idea for the degradation of PRO.
基金supported by the National Natural Science Foundation of China(Nos 82256147,82072178)Support Program for Growth Factor Research(No.SZYZ-TR-06)GuangDong Basic and Applied Basic Research Foundation(No.2023A1515111087).
文摘Background:Diabetic foot ulcer(DFU)is one of the most common and complex complications of diabetes,but the underlying pathophysiology remains unclear.Single-cell RNA sequencing(scRNA-seq)has been conducted to explore novel cell types or molecular profiles of DFU from various perspectives.This study aimed to comprehensively analyze the potential mechanisms underlying impaired re-epithelization of DFU in a single-cell perspective.Methods:We conducted scRNA-seq on tissues from human normal skin,acute wound,and DFU to investigate the potential mechanisms underlying impaired epidermal differentiation and the pathological microenvironment.Pseudo-time and lineage inference analyses revealed the distinct states and transition trajectories of epidermal cells under different conditions.Transcription factor analysis revealed the potential regulatory mechanism of key subtypes of keratinocytes.Cell-cell interaction analysis revealed the regulatory network between the proinflammatory microenvironment and epidermal cells.Laser-capture microscopy coupled with RNA sequencing(LCM-seq)and multiplex immunohistochemistry were used to validate the expression and location of key subtypes of keratinocytes.Results:Our research provided a comprehensive map of the phenotypic and dynamic changes that occur during epidermal differentiation,alongside the corresponding regulatory networks in DFU.Importantly,we identified two subtypes of keratinocytes:basal cells(BC-2)and diabetes-associated keratinocytes(DAK)that might play crucial roles in the impairment of epidermal homeostasis.BC-2 and DAK showed a marked increase in DFU,with an inactive state and insufficient motivation for epidermal differentiation.BC-2 was involved in the cellular response and apoptosis processes,with high expression of TXNIP,IFITM1,and IL1R2.Additionally,the pro-differentiation transcription factors were downregulated in BC-2 in DFU,indicating that the differentiation process might be inhibited in BC-2 in DFU.DAK was associated with cellular glucose homeostasis.Furthermore,increased CCL2+CXCL2+fibroblasts,VWA1+vascular endothelial cells,and GZMA+CD8+T cells were detected in DFU.These changes in the wound microenvironment could regulate the fate of epidermal cells through the TNFSF12-TNFRSF12A,IFNG-IFNGR1/2,and IL-1B-IL1R2 pathways,which might result in persistent inflammation and impaired epidermal differentiation in DFU.Conclusions:Our findings offer novel insights into the pathophysiology of DFU and present potential therapeutic targets that could improve wound care and treatment outcomes for DFU patients.
基金financial support from National Key R&D Program of China(No.2022YFC3320700)National Natural Science Foundation of China(Nos.62271299 and 22302118)China Postdoctoral Science Foundation(No.2022M712019)。
文摘The surficial inherent properties of TiO_(2)like exposed facet and crystalline state are vital for their surface reactions.However,efficiently controlling the specific crystal structure and the exposed crystal surface still faces big challenge.Here,the controlled solid phase transition of amorphous TiO_(2)to crystalline phase with exposed crystal facet(001)is achieved by photo-assisted atomic layer deposition(ALD)Pt process.Significantly,the obtained Pt/TiO_(2)film via photo-assisted treatment exhibits high sensing performance to NO and HF,and shows a lower optimized working temperature.The enhanced sensing performance is attributed to the metal-support strong interaction under oxidative atmosphere(O-SMSI).The facet effects leading to the unique distribution of charges at the interface combined with the catalytic effects result in the high sensing performance.This work provides a novel phase transition engineering strategy for regulating TiO_(2)from amorphous to crystalline phase,and the controllable synthesis of high Pt monatomic loading on TiO_(2)via ALD,which are critical for the accurate synthesis of efficient sensing and catalytic nanomaterials.
基金the financial support from the National Natural Science Foundation of China(Grant No.61875034)the 111 Project(Grant No.B14039)Seeding Project of Scientific and Technical Innovation of Sichuan Province(Grant No.2020107).
文摘Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.
