One novel（named palcernuine, 1） and five known cernuane-type（2–6） alkaloids were isolated from the whole plant of Palhinhaea cernua f. sikkimensis. The structure of 1, possessing an unprecedented [5/6/6/6]-tetrac...One novel（named palcernuine, 1） and five known cernuane-type（2–6） alkaloids were isolated from the whole plant of Palhinhaea cernua f. sikkimensis. The structure of 1, possessing an unprecedented [5/6/6/6]-tetracyclic ring system containing two nitrogen atoms, was established on the basis of spectroscopic methods, and its absolute configuration was determined by comparison of the experimental and calculated electronic circular dichroism（ECD） spectra. A plausible biosynthetic pathway to 1 is proposed.展开更多
Recently,a novel protein language model(PLM)was published by Liang Hong group in Science Advances1,introducing PRIME(PRotein language model for Intelligent Masked pretraining and Environment prediction,Fig.1).PRIME is...Recently,a novel protein language model(PLM)was published by Liang Hong group in Science Advances1,introducing PRIME(PRotein language model for Intelligent Masked pretraining and Environment prediction,Fig.1).PRIME is a deep learning model designed to predict and improve protein stability and activity without relying on experimental mutagenesis data.This innovative approach leverages a vast dataset of 96 million protein sequences annotated with their host bacterial optimal growth temperatures(OGTs)to develop a model that effectively guides protein engineering across various applications.展开更多
基金supported by NSFC grants (Nos. 21472021, 81273401, 81202420)grants from the Ph.D. Programs Foundation of Ministry of Education (MOE) of China (Nos. 20120071110049, 20120071120049)the National Basic Research Program of China (973 Program, No. 2013CB530700)
文摘One novel（named palcernuine, 1） and five known cernuane-type（2–6） alkaloids were isolated from the whole plant of Palhinhaea cernua f. sikkimensis. The structure of 1, possessing an unprecedented [5/6/6/6]-tetracyclic ring system containing two nitrogen atoms, was established on the basis of spectroscopic methods, and its absolute configuration was determined by comparison of the experimental and calculated electronic circular dichroism（ECD） spectra. A plausible biosynthetic pathway to 1 is proposed.
文摘Recently,a novel protein language model(PLM)was published by Liang Hong group in Science Advances1,introducing PRIME(PRotein language model for Intelligent Masked pretraining and Environment prediction,Fig.1).PRIME is a deep learning model designed to predict and improve protein stability and activity without relying on experimental mutagenesis data.This innovative approach leverages a vast dataset of 96 million protein sequences annotated with their host bacterial optimal growth temperatures(OGTs)to develop a model that effectively guides protein engineering across various applications.
