Genistein(GEN)is the most abundant aglycone in soy isoflavones and is a key factor distinguishing soybeans from other food sources.It holds significant nutritional value and exhibits notable biological activity.Howeve...Genistein(GEN)is the most abundant aglycone in soy isoflavones and is a key factor distinguishing soybeans from other food sources.It holds significant nutritional value and exhibits notable biological activity.However,the low water solubility of genistein limits its widespread application.To improve its water solubility,this study preparedγ-cyclodextrin(γ-CD)/GEN inclusion complexes and investigated their mechanism of action in allevi-ating hydrogen peroxide-induced oxidative stress damage in PC12 cells.The results showed thatγ-CD/GEN could be uniformly and stably dispersed in the system.Cell experiments indicated thatγ-CD/GEN significantly increased cell survival after treatment with organic hydrogen peroxide,and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2(Nrf2)and the expression of glutathione peroxidase 4(GPx4).It also improved mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)levels,thereby allevi-ating cell damage caused by oxidative stress.Further Nrf2 knockdown experiments revealed thatγ-CD/GEN failed to provide protection,and no protective effect was observed under RSL3 intervention.In conclusion,γ-CD/GEN exerts antioxidant effects by activating the Nrf2/GPx4 pathway,thereby reducing oxidative stress damage induced by organic hydrogen peroxide,providing a theoretical basis for the development of novel antioxidants.展开更多
Arginine demonstrates enhanced tissue repair capabilities,which may help alleviate intestinal barrier damage caused by pharmaceutical agents or other external factors.However,the intestinal barrier repair potential of...Arginine demonstrates enhanced tissue repair capabilities,which may help alleviate intestinal barrier damage caused by pharmaceutical agents or other external factors.However,the intestinal barrier repair potential of arginine-terminated egg white peptides remains undetermined.In this study,we investigated the therapeutic effects of Leu-Phe-Arg(LFR)on dextran sulfate sodium(DSS)-induced intestinal barrier damage and elucidated its underlying mechanisms.Following 7-day DSS administration,damage models developed colitis manifestations including weight loss,rectal bleeding,and elevated disease activity index(DAI)scores.Therapeutic intervention with LFR demonstrated significant attenuation of oxidative stress,suppression of inflammatory mediators,and restoration of intestinal barrier integrity.Integrated transcriptomic and proteomic analyses revealed LFR’s regulatory effects on colitis-associated pathways and amino acid metabolic networks.Notably,LFR administration downregulated key targets(PI3K,AKT,mTOR,and p70S6K)of the PI3K/AKT/mTOR/p70S6K signaling pathway.These findings provide novel mechanistic insights into the barrier-repairing properties of arginine-terminated bioactive peptides.展开更多
Synaptic dysfunction is a precursor of cognitive decline and memory impairment in neurodegenerative diseases.The complement component 1q(C1q),and complement pathway contribute to synaptic loss and plasticity,leading t...Synaptic dysfunction is a precursor of cognitive decline and memory impairment in neurodegenerative diseases.The complement component 1q(C1q),and complement pathway contribute to synaptic loss and plasticity,leading to nerve damage.The walnut-derived peptide TWLPLPR(TW-7)has beneficial effect on nerve protection.However,its specific mechanisms are still not fully understood,particularly in C1q-mediated synaptic plasticity.We investigated the neuroprotective effects of TW-7,and the mechanism of C1q-mediated synaptic plasticity in hydrogen peroxide(H_(2)O_(2))-induced HT22 cells.Treatment with C1q gene plasmid overexpression in HT22 cells showed that TW-7 reduced C1q expression,thus decreasing complement three(C3)expression and increasing the presynaptic protein SYP and postsynaptic protein PSD95.Molecular docking confirmed that TW-7 binding to the recognition site of C1q and reduce synaptic loss.Furthermore,TW-7 inhibited local apoptosis and mediated the phosphoinositide 3-kinase/Programmable Logic Controller/IP3 receptor pathway to maintain intracellular Ca^(2+)homeostasis and improve synaptic plasticity.Our results suggest that TW-7 exerts a neuroprotective effect in HT22 cells by C1q-mediated synaptic plasticity.展开更多
The post-maturation of fermented beverages is extremely vital because it could influence the products’quality and bioactive ingredients.This work aimed to obtain fermented egg-milk beverage(FEMB)and investigate the i...The post-maturation of fermented beverages is extremely vital because it could influence the products’quality and bioactive ingredients.This work aimed to obtain fermented egg-milk beverage(FEMB)and investigate the impact of different cooling times during post-maturation on physicochemical and texture properties.Herein,chemical analyzing methods such as acidity titration,texture and water holding capacity determination,microbiological analysis,and light microscopy were utilized to explore the properties of FEMB samples with different cooling times.Besides,the functional antioxidant properties were considered in this part.The results revealed that with the extension of cooling time(1-7 h),the acidity rose from 64.00±1.00°T to 82.00±2.08°T(increased by 28.12%),and the water holding rate decreased from 85.67%±1.28%-76.98%±0.13%.The number of lactic acid bacteria colonies increased to 52.00±4.58×10^(7) CFU/mL first and then decreased to 23.30±2.51×10^(7) CFU/mL.With the prolong of the cooling time,the antioxidant capacity was increased.The results of light microscopy proved that protein aggregation is one of the reasons for the sticky texture.Combined with the cooling time and fermented beverage properties,this paper better understands the physicochemical and texture properties of FEMB in the period of post-maturation,which could provide a new perspective for fermented beverages processing.展开更多
基金supported by the National Natural Science Foundation of China(No.32201935)the Education Department of Jilin Province(No.JJKH20241310KJ)+1 种基金the Jilin Province Postdoctoral Funding(Sub-sidy)Projectthe Development Program of the Science and Technology of Jilin Province(No.20220204087YY).
文摘Genistein(GEN)is the most abundant aglycone in soy isoflavones and is a key factor distinguishing soybeans from other food sources.It holds significant nutritional value and exhibits notable biological activity.However,the low water solubility of genistein limits its widespread application.To improve its water solubility,this study preparedγ-cyclodextrin(γ-CD)/GEN inclusion complexes and investigated their mechanism of action in allevi-ating hydrogen peroxide-induced oxidative stress damage in PC12 cells.The results showed thatγ-CD/GEN could be uniformly and stably dispersed in the system.Cell experiments indicated thatγ-CD/GEN significantly increased cell survival after treatment with organic hydrogen peroxide,and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2(Nrf2)and the expression of glutathione peroxidase 4(GPx4).It also improved mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)levels,thereby allevi-ating cell damage caused by oxidative stress.Further Nrf2 knockdown experiments revealed thatγ-CD/GEN failed to provide protection,and no protective effect was observed under RSL3 intervention.In conclusion,γ-CD/GEN exerts antioxidant effects by activating the Nrf2/GPx4 pathway,thereby reducing oxidative stress damage induced by organic hydrogen peroxide,providing a theoretical basis for the development of novel antioxidants.
基金funded by the National Key R&D Program of China(2022YFD2101002).
文摘Arginine demonstrates enhanced tissue repair capabilities,which may help alleviate intestinal barrier damage caused by pharmaceutical agents or other external factors.However,the intestinal barrier repair potential of arginine-terminated egg white peptides remains undetermined.In this study,we investigated the therapeutic effects of Leu-Phe-Arg(LFR)on dextran sulfate sodium(DSS)-induced intestinal barrier damage and elucidated its underlying mechanisms.Following 7-day DSS administration,damage models developed colitis manifestations including weight loss,rectal bleeding,and elevated disease activity index(DAI)scores.Therapeutic intervention with LFR demonstrated significant attenuation of oxidative stress,suppression of inflammatory mediators,and restoration of intestinal barrier integrity.Integrated transcriptomic and proteomic analyses revealed LFR’s regulatory effects on colitis-associated pathways and amino acid metabolic networks.Notably,LFR administration downregulated key targets(PI3K,AKT,mTOR,and p70S6K)of the PI3K/AKT/mTOR/p70S6K signaling pathway.These findings provide novel mechanistic insights into the barrier-repairing properties of arginine-terminated bioactive peptides.
基金supported by the Science and Technology Research Project of Jilin Province(grant number:JJKH20220347KJ)the Science and Technology Development Project of Jilin Province(grant number:20230202055NC).
文摘Synaptic dysfunction is a precursor of cognitive decline and memory impairment in neurodegenerative diseases.The complement component 1q(C1q),and complement pathway contribute to synaptic loss and plasticity,leading to nerve damage.The walnut-derived peptide TWLPLPR(TW-7)has beneficial effect on nerve protection.However,its specific mechanisms are still not fully understood,particularly in C1q-mediated synaptic plasticity.We investigated the neuroprotective effects of TW-7,and the mechanism of C1q-mediated synaptic plasticity in hydrogen peroxide(H_(2)O_(2))-induced HT22 cells.Treatment with C1q gene plasmid overexpression in HT22 cells showed that TW-7 reduced C1q expression,thus decreasing complement three(C3)expression and increasing the presynaptic protein SYP and postsynaptic protein PSD95.Molecular docking confirmed that TW-7 binding to the recognition site of C1q and reduce synaptic loss.Furthermore,TW-7 inhibited local apoptosis and mediated the phosphoinositide 3-kinase/Programmable Logic Controller/IP3 receptor pathway to maintain intracellular Ca^(2+)homeostasis and improve synaptic plasticity.Our results suggest that TW-7 exerts a neuroprotective effect in HT22 cells by C1q-mediated synaptic plasticity.
基金funded by National Key R&D Program of China(2022YFD2101002)Jilin Province Science and Technology Youth Talent Support Project(QT202021).
文摘The post-maturation of fermented beverages is extremely vital because it could influence the products’quality and bioactive ingredients.This work aimed to obtain fermented egg-milk beverage(FEMB)and investigate the impact of different cooling times during post-maturation on physicochemical and texture properties.Herein,chemical analyzing methods such as acidity titration,texture and water holding capacity determination,microbiological analysis,and light microscopy were utilized to explore the properties of FEMB samples with different cooling times.Besides,the functional antioxidant properties were considered in this part.The results revealed that with the extension of cooling time(1-7 h),the acidity rose from 64.00±1.00°T to 82.00±2.08°T(increased by 28.12%),and the water holding rate decreased from 85.67%±1.28%-76.98%±0.13%.The number of lactic acid bacteria colonies increased to 52.00±4.58×10^(7) CFU/mL first and then decreased to 23.30±2.51×10^(7) CFU/mL.With the prolong of the cooling time,the antioxidant capacity was increased.The results of light microscopy proved that protein aggregation is one of the reasons for the sticky texture.Combined with the cooling time and fermented beverage properties,this paper better understands the physicochemical and texture properties of FEMB in the period of post-maturation,which could provide a new perspective for fermented beverages processing.
