[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extr...[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions, including pH, solid-liquid ratio, extraction duration and active agent concentra- tion. The extracted phytase was purified with salting-out concentration method for SDS-PAGE eIectrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634 -2009. [ Result] Phytase activity reached the highest under extraction conditions of pH 5.0, solid-liquid ratio 10, room temperature, shaking speed 200 r/min and shaking duration 1 h, without addition of active agems. [ Condusion] This study improved the extraction technology of phytase from wheat plants and was suitable for practical ap- plication.展开更多
Neoantigen-based immunotherapy has demonstrated long-lasting antitumor activity.The recognition of neoantigens by T cell receptors(TCRs)is considered a trigger for antitumor responses.Due to the overwhelming number of...Neoantigen-based immunotherapy has demonstrated long-lasting antitumor activity.The recognition of neoantigens by T cell receptors(TCRs)is considered a trigger for antitumor responses.Due to the overwhelming number of TCR repertoires in the human genome,pinpointing neoantigen-specific TCRs is a formidable challenge.Recent studies have identified a number of functional neoantigen-specific TCRs,but the corresponding information is scattered across published literature and is difficult to retrieve.To improve access to these data,we developed an immunoinformatic database(NeoTCR)containing a unified description of publicly available neoantigen-specific TCR sequences,as well as relevant information on targeted neoantigens,from experimentally-supported studies across 17 cancer subtypes.A user-friendly web interface allows interactive browsing and running of complex database queries.To facilitate rapid identification of neoantigen-specific TCRs from raw sequencing data,NeoTCR offers a one-stop analysis for annotation and visualization of TCR clonotypes,discovery of existing neoantigen-specific TCRs,and exclusion of bystander virus-associated TCRs.NeoTCR represents a unique tool to expedite future studies of neoantigen-specific TCRs and the development of neoantigen-based immunotherapy.NeoTCR is available at http://neotcrdb.bioxai.cn/and https://github.com/lyot vincent/NeoTCR.展开更多
基金Supported by Doctoral Scientific Research Start-up Fund of Harbin Normal University "Molecular Genetic Research of Black-grain Wheat Pigment"(08XBSK87)
文摘[ Objective] This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [ Method] Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions, including pH, solid-liquid ratio, extraction duration and active agent concentra- tion. The extracted phytase was purified with salting-out concentration method for SDS-PAGE eIectrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634 -2009. [ Result] Phytase activity reached the highest under extraction conditions of pH 5.0, solid-liquid ratio 10, room temperature, shaking speed 200 r/min and shaking duration 1 h, without addition of active agems. [ Condusion] This study improved the extraction technology of phytase from wheat plants and was suitable for practical ap- plication.
基金supported by the National Natural Science Foundation of China(Grant No.81890994)the National Key R&D Program of China(Grant No.2019YFA0905902)+2 种基金the Guangdong Basic and Applied Basic Research Foundation,China(Grant Nos.2019A1515010299 and 2023A1515030298)the Science and Technology Program of Guangzhou,China(Grant No.202102020727)the Innovative Research Team of High-level Local Universities in Shanghai,and the Dean’s fund of Zhujiang Hospital of Southern Medical University,China(Grant No.yzjj2019qn05).
文摘Neoantigen-based immunotherapy has demonstrated long-lasting antitumor activity.The recognition of neoantigens by T cell receptors(TCRs)is considered a trigger for antitumor responses.Due to the overwhelming number of TCR repertoires in the human genome,pinpointing neoantigen-specific TCRs is a formidable challenge.Recent studies have identified a number of functional neoantigen-specific TCRs,but the corresponding information is scattered across published literature and is difficult to retrieve.To improve access to these data,we developed an immunoinformatic database(NeoTCR)containing a unified description of publicly available neoantigen-specific TCR sequences,as well as relevant information on targeted neoantigens,from experimentally-supported studies across 17 cancer subtypes.A user-friendly web interface allows interactive browsing and running of complex database queries.To facilitate rapid identification of neoantigen-specific TCRs from raw sequencing data,NeoTCR offers a one-stop analysis for annotation and visualization of TCR clonotypes,discovery of existing neoantigen-specific TCRs,and exclusion of bystander virus-associated TCRs.NeoTCR represents a unique tool to expedite future studies of neoantigen-specific TCRs and the development of neoantigen-based immunotherapy.NeoTCR is available at http://neotcrdb.bioxai.cn/and https://github.com/lyot vincent/NeoTCR.
