Objective:Lung metastasis is a common and fatal complication of liver transplantation for hepatocellular carcinoma(HCC).The precise prediction of post-transplant lung metastasis in the early phase is of great value.Me...Objective:Lung metastasis is a common and fatal complication of liver transplantation for hepatocellular carcinoma(HCC).The precise prediction of post-transplant lung metastasis in the early phase is of great value.Methods:The mRNA profiles of primary and paired lung metastatic lesions were analyzed to determine key signaling pathways.We enrolled 241 HCC patients who underwent liver transplantation from three centers.Tissue microarrays were used to evaluate the prognostic capacity of tumor necrosis factor(TNF),tumor necrosis factor receptor 1(TNFR1),and TNFR2,particularly for post-transplant lung metastasis.Results:Comparison of primary and lung metastatic lesions revealed that the TNF-dependent signaling pathway was related to lung metastasis of HCC.The expression of TNF was degraded in comparison to that in para-tumor tissues(P<0.001).The expression of key receptors in the TNF-dependent signaling pathway,TNFR1 and TNFR2,was higher in HCC tissues than in para-tumor tissues(P<0.001).TNF and TNFR1 showed no relationship with patients’outcomes,whereas elevated TNFR2 in tumor tissue was significantly associated with worse overall survival(OS)and increased recurrence risk(5-year OS rate:31.9%vs.62.5%,P<0.001).Notably,elevated TNFR2 levels were also associated with an increased risk of post-transplant lung metastasis(hazard ratio:1.146;P<0.001).Cox regression analysis revealed that TNFR2,Hangzhou criteria,age,and hepatitis B surface antigen were independent risk factors for post-transplant lung metastasis,and a novel nomogram was established accordingly.The nomogram achieved excellent prognostic efficiency(area under time-dependent receiver operating characteristic=0.755,concordance-index=0.779)and was superior to conventional models,such as the Milan criteria.Conclusions:TNFR2 is a potent prognostic biomarker for predicting post-transplant lung metastasis in patients with HCC.A nomogram incorporating TNFR2 deserves to be a helpful prognostic tool in liver transplantation for HCC.展开更多
The intensifying challenges posed by climate change and the depletion of fossil fuels have spurred concerted global efforts to develop alternative energy storage solutions.Aqueous zinc-ion batteries(AZIBs)have emerged...The intensifying challenges posed by climate change and the depletion of fossil fuels have spurred concerted global efforts to develop alternative energy storage solutions.Aqueous zinc-ion batteries(AZIBs)have emerged as promising candidates for large-scale electrochemical energy storage systems because of their intrinsic safety,cost-effectiveness,and environmental sustainability.However,Zn dendrite growth consis-tently poses a remarkable challenge to the performance improvement and commercial viability of AZIBs.The use of three-dimensional porous Zn anodes instead of planar Zn plates has been demonstrated as an effec-tive strategy to regulate the deposition/stripping behavior of Zn2+ions,thereby inhibiting the dendrite growth.Here,the merits of porous Zn anodes were summarized,and a comprehensive overview of the recent advancements in the engineering of porous Zn metal anodes was provided,with a particular emphasis on the structural orderliness and critical role of porous structure modulation in enhancing battery performance.Furthermore,strategic insights into the design of porous Zn anodes were presented to facilitate the practical implementation of AZIBs for grid-scale energy storage applications.展开更多
Rechargeable aqueous metal-ion batteries(AMBs)have attracted extensive scientific and commercial interest due to their potential for cost-effective,highly safe,and scalable stationary energy storage.However,their limi...Rechargeable aqueous metal-ion batteries(AMBs)have attracted extensive scientific and commercial interest due to their potential for cost-effective,highly safe,and scalable stationary energy storage.However,their limited output voltage,inadequate energy density,and poor reversibility of ambiguous electrode reactions in aqueous electrolytes strongly limit their practical viability.This review aims to elucidate the challenges of existing AMBs from the material design to whole device applications.We summarize the emerging electrochemistry,fundamental properties,and key issues in interfacial behaviors of various classes of prevailing AMBs,including aqueous alkali metal-ion batteries and multivalent-ion batteries,and present an appraisal of recent advances for addressing the performance deficiency.Specifically,the progress of zinc-ion batteries is highlighted to provide a ubiquitous guideline for their commercialization in the grid-scale energy storage.Finally,we figure out the dominating general challenges for achieving high-performance AMBs,laying out a perspective for future breakthroughs.展开更多
A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throu...A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts encoded.Then,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample.This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per minute.By fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc.展开更多
Heat moisture treatment is safe and non-chemical use method for modifying physicochemical properties and digestibility of starch.Slow starch digestion in rice product would provide numerous health benefi ts for the co...Heat moisture treatment is safe and non-chemical use method for modifying physicochemical properties and digestibility of starch.Slow starch digestion in rice product would provide numerous health benefi ts for the consumers.This study investigated the heat-moisture treatment to the raw paddy rice at 60 and 80ºC for 4 and 24 h,and their effects on the attributes of cooked rice.The fi rmness,adhesiveness,starch hydrolysis and morphological changes during in vitro digestion were examined.Results showed that heat-moisture treatment to raw paddy rice for 24 h signifi cantly reduced the moisture content and could increase the total starch content of the rice grain.The apparent amylose content ranged from 23.83%to 25.65%at the variation in the values between treated and untreated rice.Enhancement in the firmness and reduction in the starch hydrolysis was found in rice treated by longer heating time of 24 h.Meanwhile,the adhesiveness of cooked rice was decreased when increasing the heating temperature.The morphological observation showed that the treated rice exhibited a honey-comb-like structure during simulated gastric digestion,while the untreated rice displayed a non-uniform structure.Noteworthy,the porous structure observed in all digested rice grains during simulated small intestinal digestion could favor the starch hydrolysis.This study showed that the heat-moisture treatment to the raw paddy rice at certain conditions obviously influenced the attributes of cooked rice,especially on the starch hydrolysis,which would provide a fundamental knowledge to enable development and improvement of slow digestion rice products.展开更多
基金the National Key Research and Development Program of China(No.2021YFA1100500)the Major Research Plan of the National Natural Science Foundation of China(No.92159202)+2 种基金the Key Research&Development Plan of Zhejiang Province(No.2019C03050),the Key Research&Development Plan of Zhejiang Province(No.2021C03118)National S&T Major Project(No.2017ZX10203205)the Health Science&Technology Plan of Zhejiang Province(No.2022RC060)。
文摘Objective:Lung metastasis is a common and fatal complication of liver transplantation for hepatocellular carcinoma(HCC).The precise prediction of post-transplant lung metastasis in the early phase is of great value.Methods:The mRNA profiles of primary and paired lung metastatic lesions were analyzed to determine key signaling pathways.We enrolled 241 HCC patients who underwent liver transplantation from three centers.Tissue microarrays were used to evaluate the prognostic capacity of tumor necrosis factor(TNF),tumor necrosis factor receptor 1(TNFR1),and TNFR2,particularly for post-transplant lung metastasis.Results:Comparison of primary and lung metastatic lesions revealed that the TNF-dependent signaling pathway was related to lung metastasis of HCC.The expression of TNF was degraded in comparison to that in para-tumor tissues(P<0.001).The expression of key receptors in the TNF-dependent signaling pathway,TNFR1 and TNFR2,was higher in HCC tissues than in para-tumor tissues(P<0.001).TNF and TNFR1 showed no relationship with patients’outcomes,whereas elevated TNFR2 in tumor tissue was significantly associated with worse overall survival(OS)and increased recurrence risk(5-year OS rate:31.9%vs.62.5%,P<0.001).Notably,elevated TNFR2 levels were also associated with an increased risk of post-transplant lung metastasis(hazard ratio:1.146;P<0.001).Cox regression analysis revealed that TNFR2,Hangzhou criteria,age,and hepatitis B surface antigen were independent risk factors for post-transplant lung metastasis,and a novel nomogram was established accordingly.The nomogram achieved excellent prognostic efficiency(area under time-dependent receiver operating characteristic=0.755,concordance-index=0.779)and was superior to conventional models,such as the Milan criteria.Conclusions:TNFR2 is a potent prognostic biomarker for predicting post-transplant lung metastasis in patients with HCC.A nomogram incorporating TNFR2 deserves to be a helpful prognostic tool in liver transplantation for HCC.
基金National Natural Science Foundation of China(Grant No.22309102)China Postdoctoral Science Foundation(Grant No.2022M711788)+3 种基金National Key Research and Development Program of China(Grant No.2022YFB2404500)Fundamental Research Project of Shenzhen(Grant No.JCYJ20230807111702005)the Australian Research Council through the ARC Discovery Project(Grant No.DP230101579)ACR Linkage Project(Grant No.LP200200926).
文摘The intensifying challenges posed by climate change and the depletion of fossil fuels have spurred concerted global efforts to develop alternative energy storage solutions.Aqueous zinc-ion batteries(AZIBs)have emerged as promising candidates for large-scale electrochemical energy storage systems because of their intrinsic safety,cost-effectiveness,and environmental sustainability.However,Zn dendrite growth consis-tently poses a remarkable challenge to the performance improvement and commercial viability of AZIBs.The use of three-dimensional porous Zn anodes instead of planar Zn plates has been demonstrated as an effec-tive strategy to regulate the deposition/stripping behavior of Zn2+ions,thereby inhibiting the dendrite growth.Here,the merits of porous Zn anodes were summarized,and a comprehensive overview of the recent advancements in the engineering of porous Zn metal anodes was provided,with a particular emphasis on the structural orderliness and critical role of porous structure modulation in enhancing battery performance.Furthermore,strategic insights into the design of porous Zn anodes were presented to facilitate the practical implementation of AZIBs for grid-scale energy storage applications.
基金supported by National Key Research and Development Program of China(2022YFB2404500)Shenzhen Outstanding Talents Training Fund。
文摘Rechargeable aqueous metal-ion batteries(AMBs)have attracted extensive scientific and commercial interest due to their potential for cost-effective,highly safe,and scalable stationary energy storage.However,their limited output voltage,inadequate energy density,and poor reversibility of ambiguous electrode reactions in aqueous electrolytes strongly limit their practical viability.This review aims to elucidate the challenges of existing AMBs from the material design to whole device applications.We summarize the emerging electrochemistry,fundamental properties,and key issues in interfacial behaviors of various classes of prevailing AMBs,including aqueous alkali metal-ion batteries and multivalent-ion batteries,and present an appraisal of recent advances for addressing the performance deficiency.Specifically,the progress of zinc-ion batteries is highlighted to provide a ubiquitous guideline for their commercialization in the grid-scale energy storage.Finally,we figure out the dominating general challenges for achieving high-performance AMBs,laying out a perspective for future breakthroughs.
基金This research has received funding support from the 1000 Youth Talents Plan of China(P.F.)the Fundamental Research Program of Shenzhen(P.F.,JCYJ20160429182424047)+2 种基金and the National Heart Lung and Blood Institute[R01HL111437(T.K.H.)R01HL083015(T.K.H.),R01HL118650(T.K.H.)and EB U54 EB0220002(T.K.H.)].
文摘A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts encoded.Then,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample.This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per minute.By fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc.
基金supported by a grant from the Project of the NARO Bio-oriented Technology Research Advancement Institution for Research Program on Development of Innovative Technology.The grant number was 01010B.
文摘Heat moisture treatment is safe and non-chemical use method for modifying physicochemical properties and digestibility of starch.Slow starch digestion in rice product would provide numerous health benefi ts for the consumers.This study investigated the heat-moisture treatment to the raw paddy rice at 60 and 80ºC for 4 and 24 h,and their effects on the attributes of cooked rice.The fi rmness,adhesiveness,starch hydrolysis and morphological changes during in vitro digestion were examined.Results showed that heat-moisture treatment to raw paddy rice for 24 h signifi cantly reduced the moisture content and could increase the total starch content of the rice grain.The apparent amylose content ranged from 23.83%to 25.65%at the variation in the values between treated and untreated rice.Enhancement in the firmness and reduction in the starch hydrolysis was found in rice treated by longer heating time of 24 h.Meanwhile,the adhesiveness of cooked rice was decreased when increasing the heating temperature.The morphological observation showed that the treated rice exhibited a honey-comb-like structure during simulated gastric digestion,while the untreated rice displayed a non-uniform structure.Noteworthy,the porous structure observed in all digested rice grains during simulated small intestinal digestion could favor the starch hydrolysis.This study showed that the heat-moisture treatment to the raw paddy rice at certain conditions obviously influenced the attributes of cooked rice,especially on the starch hydrolysis,which would provide a fundamental knowledge to enable development and improvement of slow digestion rice products.
