A series of novel hindered amine light stabilizers containing an N-halamine moiety were designed and synthesized. Their structures were characterized by FT-IR, 1H NMR, and MS. The compounds were tested for antibacteri...A series of novel hindered amine light stabilizers containing an N-halamine moiety were designed and synthesized. Their structures were characterized by FT-IR, 1H NMR, and MS. The compounds were tested for antibacterial activity against Candida albicans, Staphylococcus aureus, and Escherichia coll. At a concentration of 0.5 mmol/L, these compounds all exhibited satisfactory antibacterial activity against all the three types of bacteria.展开更多
Mixed matrix membranes(MMMs)could combine the advantages of both polymeric membranes and porousfillers,making them an effective alternative to conventional polymer membranes.However,interfacial incompatibility issues,s...Mixed matrix membranes(MMMs)could combine the advantages of both polymeric membranes and porousfillers,making them an effective alternative to conventional polymer membranes.However,interfacial incompatibility issues,such as the presence of interfacial voids,hardening of polymer chains,and blockage of micropores by polymers between common MMMsfillers and the polymer matrix,currently limit the gas sep-aration performance of MMMs.Ternary phase MMMs(consisting of afiller,an additive,and a matrix)made by adding a third compound,usually functionalized additives,can overcome the structural problems of binary phase MMMs and positively impact membrane separation performance.This review introduces the structure and fabrication processes for ternary MMMs,categorizes various nanofillers and the third component,and summarizes and analyzes in detail the CO_(2) separation performance of newly developed ternary MMMs based on both rubbery and glassy polymers.Based on this separation data,the challenges of ternary MMMs are also discussed.Finally,future directions for ternary MMMs are proposed.展开更多
BACKGROUND Esophageal-gastric variceal bleeding(EGVB)represents a severe complication among patients with cirrhosis and often culminates in fatal outcomes.Interven-tional therapy,a rapidly developing treatment modalit...BACKGROUND Esophageal-gastric variceal bleeding(EGVB)represents a severe complication among patients with cirrhosis and often culminates in fatal outcomes.Interven-tional therapy,a rapidly developing treatment modality over the past few years,has found widespread application in clinical practice due to its minimally inva-sive characteristics.However,whether transjugular intrahepatic portosystemic shunt(TIPS)treatment has an impact on patient prognosis remains controversial.METHODS A retrospective study was conducted on ninety-two patients presenting with cirrhotic EGVB who were admitted to our hospital between September 2020 and September 2022.Based on the different modes of treatment,the patients were assigned to the study group(TIPS received,n=50)or the control group(per-cutaneous transhepatic varices embolization received,n=42).Comparative ana-lyses were performed between the two groups preoperatively and one month postoperatively for the following parameters:Varicosity status;hemodynamic parameters[portal vein flow velocity(PVV)and portal vein diameter(PVD);platelet count(PLT);red blood cell count;white blood cell count(WBC);and hepatic function[albumin(ALB),total bilirubin(TBIL),and aspartate transaminase(AST)].The Generic Quality of Life Inventory-74 was utilized to assess quality of life in the two groups,and the 1-year postoperative rebleeding and survival rates were compared.RESULTS Following surgical intervention,there was an improvement in the incidence of varicosity compared to the preoperative status in both cohorts.Notably,the study group exhibited more pronounced enhancements than did the control group(P<0.05).PVV increased,and PVD decreased compared to the preoperative values,with the study cohort achieving better outcomes(P<0.05).PLT and WBC counts were elevated postoperatively in the two groups,with the study cohort displaying higher PLT and WBC counts(P<0.05).No differences were detected between the two groups in terms of serum ALB,TBIL,or AST levels either preoperatively or postoperatively(P<0.05).Postoperative scores across all dimensions of life quality surpassed preoperative scores,with the study cohort achieving higher scores(P<0.05).At 22.00%,the one-year postoperative rebleeding rate in the study cohort was significantly lower than that in the control group(42.86%;P<0.05);conversely,no marked difference was obser-ved in the 1-year postoperative survival rate between the two cohorts(P>0.05).CONCLUSION TIPS,which has demonstrated robust efficacy in managing cirrhotic EGVB,remarkably alleviates varicosity and improves hemodynamics in patients.This intervention not only results in a safer profile but also contributes significantly to a more favorable prognosis.展开更多
Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previo...Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L^(-1)NAA +1.00 mg·L^(-1)KT + 0.25 mg·L^(-1)TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.展开更多
The mei-yu season,typically occurring from mid-June to mid-July,on average,contributes to 32%of the annual precipitation over the Yangtze-Huai River Valley(YHRV)and represents one of the three heavy-rainfall periods i...The mei-yu season,typically occurring from mid-June to mid-July,on average,contributes to 32%of the annual precipitation over the Yangtze-Huai River Valley(YHRV)and represents one of the three heavy-rainfall periods in China.Here,we briefly review the large-scale background,synoptic pattern,moisture transport,and cloud and precipitation characteristics of the mei-yu frontal systems in the context of the ongoing Integrative Monsoon Frontal Rainfall Experiment(IMFRE)field campaign.Phase one of the campaign,IMFRE-I,was conducted from 10 June to 10 July 2018 in the middle reaches of the YHRV.Led by the Wuhan Institute of Heavy Rain(IHR)with primary support from the National Natural Science Foundation of China,IMFRE-I maximizes the use of our observational capacity enabled by a suite of ground-based and remote sensing instruments,most notably the IHR Mesoscale Heavy Rainfall Observing System(MHROS),including different wavelengths of radars,microwave radiometers,and disdrometers.The KA350(Shanxi King-Air)aircraft participating in the campaign is equipped with Ka-band cloud radar and different probes.The comprehensive datasets from both the MHROS and aircraft instruments are combined with available satellite observations and model simulations to answer the three scientific questions of IMFRE-I.Some highlights from a previously published special issue are included in this review,and we also briefly introduce the IMFRE-II field campaign,conducted during June-July 2020,where the focus was on the spatiotemporal evolutions of the mei-yu frontal systems over the middle and lower reaches of the YHRV.展开更多
DEAR EDITOR Protein arginine methyltransferases (PRMTs)are involved in many cellular processes via the arginine methylation of histone or non-histone proteins.We examined the expression patterns of prmt4,prmt7,and prm...DEAR EDITOR Protein arginine methyltransferases (PRMTs)are involved in many cellular processes via the arginine methylation of histone or non-histone proteins.We examined the expression patterns of prmt4,prmt7,and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR).Xenopus prmt4 and prmt7 were expressed in the neural crest,brain,and spinal cord,and also detected in the eye,branchial arches,and heart at the tailbud stage.Specific print9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches.Quantitative RT-PCR indicated that the expression of prmt4 and prmt7 was up-regulated during the neurula stage,whereas prmt9 maintained its low expression until the late tailbud stage,consistent with the whole-mount in situ hybridization results.Thus,the developmental expression patterns of these three print genes in Xenopus embryos provide a basis for further functional study of such genes.展开更多
Here,we analyze the characteristics and the formation mechanisms of low-level jets(LLJs)in the middle reaches of the Yangtze River during the 2010 mei-yu season using Wuhan station radiosonde data and the fifth genera...Here,we analyze the characteristics and the formation mechanisms of low-level jets(LLJs)in the middle reaches of the Yangtze River during the 2010 mei-yu season using Wuhan station radiosonde data and the fifth generation of the European Centre for Medium-Range Weather Forecasts(ERA5)reanalysis dataset.Our results show that the vertical structure of LLJs is characterized by a predominance of boundary layer jets(BLJs)concentrated at heights of 900-1200 m.The BLJs occur most frequently at 2300 LST(LST=UTC+8 hours)but are strongest at 0200 LST,with composite wind velocities>14 m s^(-1).Synoptic-system-related LLJs(SLLJs)occur most frequently at 0800 LST but are strongest at 1100LST,with composite wind velocities>12 m s^(-1).Both BLJs and SLLJs are characterized by a southwesterly wind direction,although the wind direction of SLLJs is more westerly,and northeasterly SLLJs occur more frequently than northeasterly BLJs.When Wuhan is south of the mei-yu front,the westward extension of the northwest Pacific subtropical high intensifies,and the low-pressure system in the eastern Tibetan Plateau strengthens,favoring the formation of LLJs,which are closely related to precipitation.The wind speeds on rainstorm days are greater than those on LLJ days.Our analysis of four typical heavy precipitation events shows the presence of LLJs at the center of the precipitation and on its southern side before the onset of heavy precipitation.BLJs were shown to develop earlier than SLLJs.展开更多
Bone age assessment(BAA)helps doctors determine how a child’s bones grow and develop in clinical medicine.Traditional BAA methods rely on clinician expertise,leading to time-consuming predictions and inaccurate resul...Bone age assessment(BAA)helps doctors determine how a child’s bones grow and develop in clinical medicine.Traditional BAA methods rely on clinician expertise,leading to time-consuming predictions and inaccurate results.Most deep learning-based BAA methods feed the extracted critical points of images into the network by providing additional annotations.This operation is costly and subjective.To address these problems,we propose a multi-scale attentional densely connected network(MSADCN)in this paper.MSADCN constructs a multi-scale dense connectivity mechanism,which can avoid overfitting,obtain the local features effectively and prevent gradient vanishing even in limited training data.First,MSADCN designs multi-scale structures in the densely connected network to extract fine-grained features at different scales.Then,coordinate attention is embedded to focus on critical features and automatically locate the regions of interest(ROI)without additional annotation.In addition,to improve the model’s generalization,transfer learning is applied to train the proposed MSADCN on the public dataset IMDB-WIKI,and the obtained pre-trained weights are loaded onto the Radiological Society of North America(RSNA)dataset.Finally,label distribution learning(LDL)and expectation regression techniques are introduced into our model to exploit the correlation between hand bone images of different ages,which can obtain stable age estimates.Extensive experiments confirm that our model can converge more efficiently and obtain a mean absolute error(MAE)of 4.64 months,outperforming some state-of-the-art BAA methods.展开更多
Hepatocyte growth factor(HGF)and its receptor,c-Met,play important roles in the occurrence,development,and treatment of gastric cancer(GC).This review explored the function of the HGF/c-Met signaling pathway in GC and...Hepatocyte growth factor(HGF)and its receptor,c-Met,play important roles in the occurrence,development,and treatment of gastric cancer(GC).This review explored the function of the HGF/c-Met signaling pathway in GC and its potential targeted therapeutic mechanisms.As one of the most common malignant tumors worldwide,GC has a complex pathogenesis and limited therapeutic options.Therefore,a thorough understanding of the molecular mechanism of GC is very important for the development of new therapeutic methods.The HGF/c-Met signaling pathway plays an important role in the proliferation,migration,and invasion of GC cells and has become a new therapeutic target.This review summarizes the current research progress on the role of HGF/c-Met in GC and discusses targeted therapeutic strategies targeting this signaling pathway,providing new ideas and directions for the treatment of GC.展开更多
Background:Organic semiconductors have attracted much attention due to their excellent biocompatibility,tunable electronic structure,low cost,and antimicrobial phototherapy.However,owing to the high exciton binding en...Background:Organic semiconductors have attracted much attention due to their excellent biocompatibility,tunable electronic structure,low cost,and antimicrobial phototherapy.However,owing to the high exciton binding energies,organic semiconductor is constrained by their short exciton diffusion length,leading to inefficient transportation of photogenerated carriers and deficient antibacterial capability.Methods:To address this issue,a quad-channel synergistic antibacterial nano-platform of copper sulfide/organic semiconductor(CuS/IEICO-4F)heterojunctions with enhanced photocatalytic performance is designed and manufactured,which can produce localized heat and raise the levels of extracellular reactive oxygen species under near-infrared laser irradiation.Simultaneously,the released Cu2+can consume intrabacterial glutathione,destroying the defense system and ultimately leading to bacterial inactivation.Results:In vitro antibacterial experiments demonstrate that the organic-inorganic bio-heterojunctions possess the potent antibacterial capacity and effective bacterial eradication.Conclusion:This countermeasure shows great promise for application in infectious wound regeneration.展开更多
Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(...Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(S-43) copolymers were studied in a manner analogous to that established by Hsieh and Cheng. The comonomer sequence distributions of copolymer samples were obtained. The results show that these metallocene based copolymers contain a small amount of butene and hexene, and the EE and EEE sequences are dominant.展开更多
Objective:To investigate the predictive value of of serum NSE,umbilical cord blood albumin levels in hyperbilirubinemia full-term neonates with brain injury.Methods:300 cases of hyperbilirubinemia full-term neonates i...Objective:To investigate the predictive value of of serum NSE,umbilical cord blood albumin levels in hyperbilirubinemia full-term neonates with brain injury.Methods:300 cases of hyperbilirubinemia full-term neonates in our hospital during January 2018 to November 2019 were chosen as hyperbilirubinemia group,200 cases of healthy full-term neonates who were delivered in our hospital during the same period and whose general information matched were chosen as normal control group.According to whether the hyperbilirubinemia group had brain injury,they were further divided into bilirubin encephalopathy group(n=34)and non-bilirubin encephalopathy group(n=266).NSE,umbilical cord blood albumin levels in hyperbilirubinemia group and normal control group were compared.Clinical data,NSE and umbilical cord blood albumin levels between bilirubin encephalopathy group,non-bilirubin encephalopathy group were compared.Risk factors associated with brain injury in full-term newborn with hyperbilirubinemia were analyzed by logistics regression,predictive value of NSE and umbilical cord blood albumin on brain injury in full-term newborn with hyperbilirubinemia were evaluated by ROC curve.Results:NSE level in hyperbilirubinemia group was higher than that in normal control group(P<0.05).There was no significant difference in umbilical cord blood albumin between two groups(P>0.05).Incidence of jaundice,anemia,indirect bilirubin peak,total bilirubin peak and NSE level in bilirubin encephalopathy group were higher than those in non-bilirubin encephalopathy group,level of umbilical cord blood albumin was lower than that in non-bilirubin encephalopathy group(P<0.05).Logistic regression analysis showed that jaundice,anemia,indirect bilirubin peak,total bilirubin peak,NSE and umbilical cord blood albumin within 24h after birth were risk factors for hyperbilirubinemia with brain injury(P<0.05).When NSE was 67.09 ng/mL,the yoden index was the highest,with sensitivity and specificity of 79.70%,55.88%respectively.When the umbilical cord blood albumin was 4.20mg/mL,the jorden index was the highest,sensitivity and specificity were 76.32%,82.35%respectively.Conclusion:Abnormal changes in serum NSE,umbilical cord blood albumin levels are observed in full-term neonates with hyperbilirubinemia,moreover they have value of early prediction for subsequent brain injury.展开更多
文摘A series of novel hindered amine light stabilizers containing an N-halamine moiety were designed and synthesized. Their structures were characterized by FT-IR, 1H NMR, and MS. The compounds were tested for antibacterial activity against Candida albicans, Staphylococcus aureus, and Escherichia coll. At a concentration of 0.5 mmol/L, these compounds all exhibited satisfactory antibacterial activity against all the three types of bacteria.
基金support from Sichuan Science and Technology Program(2021YFH0116)National Natural Science Foundation of China(No.52170112)DongFang Boiler Co.,Ltd.(3522015).
文摘Mixed matrix membranes(MMMs)could combine the advantages of both polymeric membranes and porousfillers,making them an effective alternative to conventional polymer membranes.However,interfacial incompatibility issues,such as the presence of interfacial voids,hardening of polymer chains,and blockage of micropores by polymers between common MMMsfillers and the polymer matrix,currently limit the gas sep-aration performance of MMMs.Ternary phase MMMs(consisting of afiller,an additive,and a matrix)made by adding a third compound,usually functionalized additives,can overcome the structural problems of binary phase MMMs and positively impact membrane separation performance.This review introduces the structure and fabrication processes for ternary MMMs,categorizes various nanofillers and the third component,and summarizes and analyzes in detail the CO_(2) separation performance of newly developed ternary MMMs based on both rubbery and glassy polymers.Based on this separation data,the challenges of ternary MMMs are also discussed.Finally,future directions for ternary MMMs are proposed.
文摘BACKGROUND Esophageal-gastric variceal bleeding(EGVB)represents a severe complication among patients with cirrhosis and often culminates in fatal outcomes.Interven-tional therapy,a rapidly developing treatment modality over the past few years,has found widespread application in clinical practice due to its minimally inva-sive characteristics.However,whether transjugular intrahepatic portosystemic shunt(TIPS)treatment has an impact on patient prognosis remains controversial.METHODS A retrospective study was conducted on ninety-two patients presenting with cirrhotic EGVB who were admitted to our hospital between September 2020 and September 2022.Based on the different modes of treatment,the patients were assigned to the study group(TIPS received,n=50)or the control group(per-cutaneous transhepatic varices embolization received,n=42).Comparative ana-lyses were performed between the two groups preoperatively and one month postoperatively for the following parameters:Varicosity status;hemodynamic parameters[portal vein flow velocity(PVV)and portal vein diameter(PVD);platelet count(PLT);red blood cell count;white blood cell count(WBC);and hepatic function[albumin(ALB),total bilirubin(TBIL),and aspartate transaminase(AST)].The Generic Quality of Life Inventory-74 was utilized to assess quality of life in the two groups,and the 1-year postoperative rebleeding and survival rates were compared.RESULTS Following surgical intervention,there was an improvement in the incidence of varicosity compared to the preoperative status in both cohorts.Notably,the study group exhibited more pronounced enhancements than did the control group(P<0.05).PVV increased,and PVD decreased compared to the preoperative values,with the study cohort achieving better outcomes(P<0.05).PLT and WBC counts were elevated postoperatively in the two groups,with the study cohort displaying higher PLT and WBC counts(P<0.05).No differences were detected between the two groups in terms of serum ALB,TBIL,or AST levels either preoperatively or postoperatively(P<0.05).Postoperative scores across all dimensions of life quality surpassed preoperative scores,with the study cohort achieving higher scores(P<0.05).At 22.00%,the one-year postoperative rebleeding rate in the study cohort was significantly lower than that in the control group(42.86%;P<0.05);conversely,no marked difference was obser-ved in the 1-year postoperative survival rate between the two cohorts(P>0.05).CONCLUSION TIPS,which has demonstrated robust efficacy in managing cirrhotic EGVB,remarkably alleviates varicosity and improves hemodynamics in patients.This intervention not only results in a safer profile but also contributes significantly to a more favorable prognosis.
基金funded by Guangdong Basic and Applied Basic Research Foundation (Grant No.2023A1515010237)the 2021 Dongguan Provincial Rural Revitalization Program (Grant No.20211800400022)+2 种基金the Guangdong Key Technology Research and Development Program (Grant Nos.2020B020220005,2022B1111040003)the Guangdong Modern Agricultural Industry Technology System Program (Grant No.2023KJ121)the South China Botanical Garden,the Chinese Academy of Sciences (Grant No.QNXM-02)。
文摘Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L^(-1)NAA +1.00 mg·L^(-1)KT + 0.25 mg·L^(-1)TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.
基金The datasets were provided by the Mesoscale Heavy Rainfall Observing System(MHROS)of the Wuhan Institute of Heave Rain(IHR),China Meteorological AdministrationThe IMFRE field campaign is primarily supported by the National Natural Science Foundation of China(Grant Nos.41620104009 and 91637211).
文摘The mei-yu season,typically occurring from mid-June to mid-July,on average,contributes to 32%of the annual precipitation over the Yangtze-Huai River Valley(YHRV)and represents one of the three heavy-rainfall periods in China.Here,we briefly review the large-scale background,synoptic pattern,moisture transport,and cloud and precipitation characteristics of the mei-yu frontal systems in the context of the ongoing Integrative Monsoon Frontal Rainfall Experiment(IMFRE)field campaign.Phase one of the campaign,IMFRE-I,was conducted from 10 June to 10 July 2018 in the middle reaches of the YHRV.Led by the Wuhan Institute of Heavy Rain(IHR)with primary support from the National Natural Science Foundation of China,IMFRE-I maximizes the use of our observational capacity enabled by a suite of ground-based and remote sensing instruments,most notably the IHR Mesoscale Heavy Rainfall Observing System(MHROS),including different wavelengths of radars,microwave radiometers,and disdrometers.The KA350(Shanxi King-Air)aircraft participating in the campaign is equipped with Ka-band cloud radar and different probes.The comprehensive datasets from both the MHROS and aircraft instruments are combined with available satellite observations and model simulations to answer the three scientific questions of IMFRE-I.Some highlights from a previously published special issue are included in this review,and we also briefly introduce the IMFRE-II field campaign,conducted during June-July 2020,where the focus was on the spatiotemporal evolutions of the mei-yu frontal systems over the middle and lower reaches of the YHRV.
基金supported by grants from the Research Grants Council of Hong Kong CUHK14167017,CUHK24100414 to H.Z.the Shenzhen Innovation Committee of Science and Technology grants(JCYJ20150331101823691)Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research(2017B030301018)to Y.D
文摘DEAR EDITOR Protein arginine methyltransferases (PRMTs)are involved in many cellular processes via the arginine methylation of histone or non-histone proteins.We examined the expression patterns of prmt4,prmt7,and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR).Xenopus prmt4 and prmt7 were expressed in the neural crest,brain,and spinal cord,and also detected in the eye,branchial arches,and heart at the tailbud stage.Specific print9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches.Quantitative RT-PCR indicated that the expression of prmt4 and prmt7 was up-regulated during the neurula stage,whereas prmt9 maintained its low expression until the late tailbud stage,consistent with the whole-mount in situ hybridization results.Thus,the developmental expression patterns of these three print genes in Xenopus embryos provide a basis for further functional study of such genes.
基金supported by the National Natural Science Foundation of China(Grant Nos.42230612,41620104009,41705019,42075186,and 41975058)the Projects of the S&T Development Foundation of the Hubei Meteorological Bureau(Grants No.2021Q04 and 2020Y04)。
文摘Here,we analyze the characteristics and the formation mechanisms of low-level jets(LLJs)in the middle reaches of the Yangtze River during the 2010 mei-yu season using Wuhan station radiosonde data and the fifth generation of the European Centre for Medium-Range Weather Forecasts(ERA5)reanalysis dataset.Our results show that the vertical structure of LLJs is characterized by a predominance of boundary layer jets(BLJs)concentrated at heights of 900-1200 m.The BLJs occur most frequently at 2300 LST(LST=UTC+8 hours)but are strongest at 0200 LST,with composite wind velocities>14 m s^(-1).Synoptic-system-related LLJs(SLLJs)occur most frequently at 0800 LST but are strongest at 1100LST,with composite wind velocities>12 m s^(-1).Both BLJs and SLLJs are characterized by a southwesterly wind direction,although the wind direction of SLLJs is more westerly,and northeasterly SLLJs occur more frequently than northeasterly BLJs.When Wuhan is south of the mei-yu front,the westward extension of the northwest Pacific subtropical high intensifies,and the low-pressure system in the eastern Tibetan Plateau strengthens,favoring the formation of LLJs,which are closely related to precipitation.The wind speeds on rainstorm days are greater than those on LLJ days.Our analysis of four typical heavy precipitation events shows the presence of LLJs at the center of the precipitation and on its southern side before the onset of heavy precipitation.BLJs were shown to develop earlier than SLLJs.
基金This research is partially supported by grant from the National Natural Science Foundation of China(No.72071019)grant from the Natural Science Foundation of Chongqing(No.cstc2021jcyj-msxmX0185)grant from the Chongqing Graduate Education and Teaching Reform Research Project(No.yjg193096).
文摘Bone age assessment(BAA)helps doctors determine how a child’s bones grow and develop in clinical medicine.Traditional BAA methods rely on clinician expertise,leading to time-consuming predictions and inaccurate results.Most deep learning-based BAA methods feed the extracted critical points of images into the network by providing additional annotations.This operation is costly and subjective.To address these problems,we propose a multi-scale attentional densely connected network(MSADCN)in this paper.MSADCN constructs a multi-scale dense connectivity mechanism,which can avoid overfitting,obtain the local features effectively and prevent gradient vanishing even in limited training data.First,MSADCN designs multi-scale structures in the densely connected network to extract fine-grained features at different scales.Then,coordinate attention is embedded to focus on critical features and automatically locate the regions of interest(ROI)without additional annotation.In addition,to improve the model’s generalization,transfer learning is applied to train the proposed MSADCN on the public dataset IMDB-WIKI,and the obtained pre-trained weights are loaded onto the Radiological Society of North America(RSNA)dataset.Finally,label distribution learning(LDL)and expectation regression techniques are introduced into our model to exploit the correlation between hand bone images of different ages,which can obtain stable age estimates.Extensive experiments confirm that our model can converge more efficiently and obtain a mean absolute error(MAE)of 4.64 months,outperforming some state-of-the-art BAA methods.
文摘Hepatocyte growth factor(HGF)and its receptor,c-Met,play important roles in the occurrence,development,and treatment of gastric cancer(GC).This review explored the function of the HGF/c-Met signaling pathway in GC and its potential targeted therapeutic mechanisms.As one of the most common malignant tumors worldwide,GC has a complex pathogenesis and limited therapeutic options.Therefore,a thorough understanding of the molecular mechanism of GC is very important for the development of new therapeutic methods.The HGF/c-Met signaling pathway plays an important role in the proliferation,migration,and invasion of GC cells and has become a new therapeutic target.This review summarizes the current research progress on the role of HGF/c-Met in GC and discusses targeted therapeutic strategies targeting this signaling pathway,providing new ideas and directions for the treatment of GC.
基金jointly funded by the National Natural Science Foundation of China(32271392,52302351)China Postdoctoral Science Foundation(2023M732477)+1 种基金Sichuan University Postdoctoral Interdisciplinary Innovation Fund(JCXK2205)Sichuan University Post-Doctor Research Project(2023SCU12116).
文摘Background:Organic semiconductors have attracted much attention due to their excellent biocompatibility,tunable electronic structure,low cost,and antimicrobial phototherapy.However,owing to the high exciton binding energies,organic semiconductor is constrained by their short exciton diffusion length,leading to inefficient transportation of photogenerated carriers and deficient antibacterial capability.Methods:To address this issue,a quad-channel synergistic antibacterial nano-platform of copper sulfide/organic semiconductor(CuS/IEICO-4F)heterojunctions with enhanced photocatalytic performance is designed and manufactured,which can produce localized heat and raise the levels of extracellular reactive oxygen species under near-infrared laser irradiation.Simultaneously,the released Cu2+can consume intrabacterial glutathione,destroying the defense system and ultimately leading to bacterial inactivation.Results:In vitro antibacterial experiments demonstrate that the organic-inorganic bio-heterojunctions possess the potent antibacterial capacity and effective bacterial eradication.Conclusion:This countermeasure shows great promise for application in infectious wound regeneration.
文摘Metallocene based polyethylenes were prepared by SMOPEC's 'metallocene adduct' technology in a gas phase fluidized bed model reactor. The C-13-NMR spectra of ethylene/1-butene (S-34) and ethylene/1-hexene(S-43) copolymers were studied in a manner analogous to that established by Hsieh and Cheng. The comonomer sequence distributions of copolymer samples were obtained. The results show that these metallocene based copolymers contain a small amount of butene and hexene, and the EE and EEE sequences are dominant.
基金Guilin Scientific Research and Technology Development Project(No.20150403-6-1)Project to improve the basic scientific research ability of young and middle-aged teachers in Guangxi Universities(No.KY2016LX247)Young and middle-aged faculty research capacity Enhancement project of Guilin Medical College(No.2018glmcy096)
文摘Objective:To investigate the predictive value of of serum NSE,umbilical cord blood albumin levels in hyperbilirubinemia full-term neonates with brain injury.Methods:300 cases of hyperbilirubinemia full-term neonates in our hospital during January 2018 to November 2019 were chosen as hyperbilirubinemia group,200 cases of healthy full-term neonates who were delivered in our hospital during the same period and whose general information matched were chosen as normal control group.According to whether the hyperbilirubinemia group had brain injury,they were further divided into bilirubin encephalopathy group(n=34)and non-bilirubin encephalopathy group(n=266).NSE,umbilical cord blood albumin levels in hyperbilirubinemia group and normal control group were compared.Clinical data,NSE and umbilical cord blood albumin levels between bilirubin encephalopathy group,non-bilirubin encephalopathy group were compared.Risk factors associated with brain injury in full-term newborn with hyperbilirubinemia were analyzed by logistics regression,predictive value of NSE and umbilical cord blood albumin on brain injury in full-term newborn with hyperbilirubinemia were evaluated by ROC curve.Results:NSE level in hyperbilirubinemia group was higher than that in normal control group(P<0.05).There was no significant difference in umbilical cord blood albumin between two groups(P>0.05).Incidence of jaundice,anemia,indirect bilirubin peak,total bilirubin peak and NSE level in bilirubin encephalopathy group were higher than those in non-bilirubin encephalopathy group,level of umbilical cord blood albumin was lower than that in non-bilirubin encephalopathy group(P<0.05).Logistic regression analysis showed that jaundice,anemia,indirect bilirubin peak,total bilirubin peak,NSE and umbilical cord blood albumin within 24h after birth were risk factors for hyperbilirubinemia with brain injury(P<0.05).When NSE was 67.09 ng/mL,the yoden index was the highest,with sensitivity and specificity of 79.70%,55.88%respectively.When the umbilical cord blood albumin was 4.20mg/mL,the jorden index was the highest,sensitivity and specificity were 76.32%,82.35%respectively.Conclusion:Abnormal changes in serum NSE,umbilical cord blood albumin levels are observed in full-term neonates with hyperbilirubinemia,moreover they have value of early prediction for subsequent brain injury.
