Objective:To evaluate the chemical compositions and biological activities of an endemic Ballota nigra L.subsp.anatolica P.H.Davis.Methods:Essential oil and fatty acid composition were determined by GC/MS analysis.ABTS...Objective:To evaluate the chemical compositions and biological activities of an endemic Ballota nigra L.subsp.anatolica P.H.Davis.Methods:Essential oil and fatty acid composition were determined by GC/MS analysis.ABTS cation radical decolourisation and cupric reducing antioxidant capacity assays were carried out to indicate the antioxidant activity.The anticholinesterase potential of the extracts were determined by Ellman method.Results:The major compounds in the fatty acid composition of the petroleum ether extract were identified as palmitic(36.0%)and linoleic acids(14.3%).The major components of essential oil were 1-hexacosanol(26.7%),germacrene-D(9.3%)and caryophyllene oxide(9.3%).The water extract indicated higher ABTS cation radical scavenging activity than α-tocopherol and BHT,at 100μg/mL.The acetone extract showed 71.58 and 44.71%inhibitory activity against butyrylcholinesterase and acetylcholinesterase enzyme at 200μg/mL,respectively.Conclusions:The water and acetone extracts of Ballota nigra subsp.anatolica can be investigated in terms of both phytochemical and biological aspects to find natural active compounds.展开更多
基金supported by the Research Fund of Dicle University(DUBAP)(No:12-ASMYO-148).
文摘Objective:To evaluate the chemical compositions and biological activities of an endemic Ballota nigra L.subsp.anatolica P.H.Davis.Methods:Essential oil and fatty acid composition were determined by GC/MS analysis.ABTS cation radical decolourisation and cupric reducing antioxidant capacity assays were carried out to indicate the antioxidant activity.The anticholinesterase potential of the extracts were determined by Ellman method.Results:The major compounds in the fatty acid composition of the petroleum ether extract were identified as palmitic(36.0%)and linoleic acids(14.3%).The major components of essential oil were 1-hexacosanol(26.7%),germacrene-D(9.3%)and caryophyllene oxide(9.3%).The water extract indicated higher ABTS cation radical scavenging activity than α-tocopherol and BHT,at 100μg/mL.The acetone extract showed 71.58 and 44.71%inhibitory activity against butyrylcholinesterase and acetylcholinesterase enzyme at 200μg/mL,respectively.Conclusions:The water and acetone extracts of Ballota nigra subsp.anatolica can be investigated in terms of both phytochemical and biological aspects to find natural active compounds.
