Ischemic stroke(IS)is a prevalent neurological disorder often resulting in significant disability or mortality.Resveratrol,extracted from Polygonum cuspidatum Sieb.et Zucc.(commonly known as Japanese knotweed),has bee...Ischemic stroke(IS)is a prevalent neurological disorder often resulting in significant disability or mortality.Resveratrol,extracted from Polygonum cuspidatum Sieb.et Zucc.(commonly known as Japanese knotweed),has been recognized for its potent neuroprotective properties.However,the neuroprotective efficacy of its derivative,(E)-4-(3,5-dimethoxystyryl)quinoline(RV02),against ischemic stroke remains inadequately explored.This study aimed to evaluate the protective effects of RV02 on neuronal ischemia-reperfusion injury both in vitro and in vivo.The research utilized an animal model of middle cerebral artery occlusion/reperfusion and SH-SY5Y cells subjected to oxygen-glucose deprivation and reperfusion to simulate ischemic conditions.The findings demonstrate that RV02 attenuates neuronal mitochondrial damage and scavenges reactive oxygen species(ROS)through mitophagy activation.Furthermore,Parkin knockdown was found to abolish RV02's ability to activate mitophagy and neuroprotection in vitro.These results suggest that RV02 shows promise as a neuroprotective agent,with the activation of Parkin-mediated mitophagy potentially serving as the primary mechanism underlying its neuroprotective effects.展开更多
基金supported by the National Natural ScienceFoundation of China (No.82174076)the Construction Project of Liaoning Provincial Key Laboratory,China (No.2022JH13/10200026)+2 种基金the Fundamental Research Funds for the Central Universities (No.N2220002)the 111 Project (No.B16009)the Research Project of Educational Commission of Liaoning Province (No.LJ212410164003)。
文摘Ischemic stroke(IS)is a prevalent neurological disorder often resulting in significant disability or mortality.Resveratrol,extracted from Polygonum cuspidatum Sieb.et Zucc.(commonly known as Japanese knotweed),has been recognized for its potent neuroprotective properties.However,the neuroprotective efficacy of its derivative,(E)-4-(3,5-dimethoxystyryl)quinoline(RV02),against ischemic stroke remains inadequately explored.This study aimed to evaluate the protective effects of RV02 on neuronal ischemia-reperfusion injury both in vitro and in vivo.The research utilized an animal model of middle cerebral artery occlusion/reperfusion and SH-SY5Y cells subjected to oxygen-glucose deprivation and reperfusion to simulate ischemic conditions.The findings demonstrate that RV02 attenuates neuronal mitochondrial damage and scavenges reactive oxygen species(ROS)through mitophagy activation.Furthermore,Parkin knockdown was found to abolish RV02's ability to activate mitophagy and neuroprotection in vitro.These results suggest that RV02 shows promise as a neuroprotective agent,with the activation of Parkin-mediated mitophagy potentially serving as the primary mechanism underlying its neuroprotective effects.
