Non-enzymatic glycation can cause the formation and accumulation of advanced glycation end products(AGEs),and it poses great threats to human health.It is urgent to search for safe and efficient inhibitors to prevent ...Non-enzymatic glycation can cause the formation and accumulation of advanced glycation end products(AGEs),and it poses great threats to human health.It is urgent to search for safe and efficient inhibitors to prevent reducing sugar induced protein glycation.In this study,we inves-tigated the anti-glycation activity and mechanism of an identified peptide,Asparagine-Tyrosine-Arginine-Arginine-Glutamic acid(NYRRE)from Chinese quince seed protein hydrolysate,by mul-tispectroscopy,confocal imaging,and computational molecular simulation.Firstly,it was found that NYRRE could scavenge hydroxyl radicals and chelate Fe 2+.Besides,the NYRRE was effective in every stage of fructose induced bovine serum albumin(BSA)glycation.The NYRRE could re-duce the formation of fructosamine,carbonyl compounds,glycoxidation products and𝛽-amyloid structure.Meanwhile,NYRRE could protect thiol groups and stabilize the spatial conformation of BSA.The NYRRE presented strong inhibition in fluorescent AGEs,and 68.19%of total AGEs formation was prevented with NYRRE at 15 mmol/L.The results of molecular simulation indi-cated that NYRRE could insert into the hydrophobic pocket of BSA and interact with hot spots,including arginine and lysine residues.The mechanism of NYRRE inhibiting protein glycation could be due to its antioxidant activity,BSA structure stabilizing ability,and specific bond with glycation sites of BSA.These results provided a valuable reference for developing NYRRE as an efficient antiglycation agent in preventing glycation-mediated diseases.展开更多
Wrinkle Floweringquince(Chaenomeles speciose(Sweet)Nakai)seed,as an unexploited forestry residue,contains considerable amount of bioactive carbohydrates with potential functionality,which was not widely concerned.The ...Wrinkle Floweringquince(Chaenomeles speciose(Sweet)Nakai)seed,as an unexploited forestry residue,contains considerable amount of bioactive carbohydrates with potential functionality,which was not widely concerned.The aim of this study is to determine the basic characterizations(molecular weight and functional group),specificcomponents(carbohydrate,protein and uronic acid contents),and functional properties of Chaenomeles speciosa seedgum(CSG).Results indicated that carbohydrate(63.80%),protein(13.69%)and uronic acid(10.30%)contents wereachieved.The CSG(average molecular weight,9.85×106 u)consists rhamnose,arabinose,xylose and glucose in a molarpercentage of 29.77꞉10.54꞉18.55꞉15.84,respectively.The Fourier transform infrared(FT-IR)analysis revealed hydroxyl,carboxyl and methyl groups andα-glycosidic linkages are founded in the CSG.The CSG was surface active and its abilityto decrease surface tension was comparable to commercial gums.Moreover,the CSG solutions showed pseudoplasticflow behavior under dynamic shear rate at high concentrations.The GSC also presented good emulsifying and foamingproperties,indicating the potential of the GSC as bioresource stabilizer and thickener in industry.展开更多
Food contamination by Escherichia coli(E.coli)is an increasing public health concern.Screening for natural plant preservatives has received increasing attention.In this study,dandelion flower phenolic extract(DFPE),wi...Food contamination by Escherichia coli(E.coli)is an increasing public health concern.Screening for natural plant preservatives has received increasing attention.In this study,dandelion flower phenolic extract(DFPE),with the strongest bacterial inhibition and the highest polyphenol level from various organs,was identified using HPLC and FTIR.The results showed a significant increase in extracellular ATP levels due to cellular membrane leakage in E.coli and decreased Na^(+)-K^(+) ATPase activity.These behaviors were caused by representative phenolic compounds such as caffeic acid in DFPE.Molecular docking simulations were performed to reveal the mechanism of interaction between caffeic acid and Na^(+)-K^(+) ATPase.This indicated that conventional hydrogen bonds,pi-anions,and pi-alkyl were involved in the interaction between them.Molecular dynamic equilibrium of the liganded ATPase complex was achieved after 20 ns.The lower values of Rg and SASA demonstrated that the liganded ATPase structure changed from a relatively loose to a tight state in the presence of caffeic acid.Overall,these findings are meaningful for screening bioactive compounds from various food-derived plant tissues using a combination of practical experimentation and molecular simulations.展开更多
基金support for this work by National Natural Science Foundation of China (No.32171732).
文摘Non-enzymatic glycation can cause the formation and accumulation of advanced glycation end products(AGEs),and it poses great threats to human health.It is urgent to search for safe and efficient inhibitors to prevent reducing sugar induced protein glycation.In this study,we inves-tigated the anti-glycation activity and mechanism of an identified peptide,Asparagine-Tyrosine-Arginine-Arginine-Glutamic acid(NYRRE)from Chinese quince seed protein hydrolysate,by mul-tispectroscopy,confocal imaging,and computational molecular simulation.Firstly,it was found that NYRRE could scavenge hydroxyl radicals and chelate Fe 2+.Besides,the NYRRE was effective in every stage of fructose induced bovine serum albumin(BSA)glycation.The NYRRE could re-duce the formation of fructosamine,carbonyl compounds,glycoxidation products and𝛽-amyloid structure.Meanwhile,NYRRE could protect thiol groups and stabilize the spatial conformation of BSA.The NYRRE presented strong inhibition in fluorescent AGEs,and 68.19%of total AGEs formation was prevented with NYRRE at 15 mmol/L.The results of molecular simulation indi-cated that NYRRE could insert into the hydrophobic pocket of BSA and interact with hot spots,including arginine and lysine residues.The mechanism of NYRRE inhibiting protein glycation could be due to its antioxidant activity,BSA structure stabilizing ability,and specific bond with glycation sites of BSA.These results provided a valuable reference for developing NYRRE as an efficient antiglycation agent in preventing glycation-mediated diseases.
基金National Key Research and Development Program of China(No.2017YFD0400902-3).
文摘Wrinkle Floweringquince(Chaenomeles speciose(Sweet)Nakai)seed,as an unexploited forestry residue,contains considerable amount of bioactive carbohydrates with potential functionality,which was not widely concerned.The aim of this study is to determine the basic characterizations(molecular weight and functional group),specificcomponents(carbohydrate,protein and uronic acid contents),and functional properties of Chaenomeles speciosa seedgum(CSG).Results indicated that carbohydrate(63.80%),protein(13.69%)and uronic acid(10.30%)contents wereachieved.The CSG(average molecular weight,9.85×106 u)consists rhamnose,arabinose,xylose and glucose in a molarpercentage of 29.77꞉10.54꞉18.55꞉15.84,respectively.The Fourier transform infrared(FT-IR)analysis revealed hydroxyl,carboxyl and methyl groups andα-glycosidic linkages are founded in the CSG.The CSG was surface active and its abilityto decrease surface tension was comparable to commercial gums.Moreover,the CSG solutions showed pseudoplasticflow behavior under dynamic shear rate at high concentrations.The GSC also presented good emulsifying and foamingproperties,indicating the potential of the GSC as bioresource stabilizer and thickener in industry.
基金supported by the National Natural Science Foundation of China[grant no.32271820]the Natural Science Foundation of Jiangsu Province[grant no.BK20181124].
文摘Food contamination by Escherichia coli(E.coli)is an increasing public health concern.Screening for natural plant preservatives has received increasing attention.In this study,dandelion flower phenolic extract(DFPE),with the strongest bacterial inhibition and the highest polyphenol level from various organs,was identified using HPLC and FTIR.The results showed a significant increase in extracellular ATP levels due to cellular membrane leakage in E.coli and decreased Na^(+)-K^(+) ATPase activity.These behaviors were caused by representative phenolic compounds such as caffeic acid in DFPE.Molecular docking simulations were performed to reveal the mechanism of interaction between caffeic acid and Na^(+)-K^(+) ATPase.This indicated that conventional hydrogen bonds,pi-anions,and pi-alkyl were involved in the interaction between them.Molecular dynamic equilibrium of the liganded ATPase complex was achieved after 20 ns.The lower values of Rg and SASA demonstrated that the liganded ATPase structure changed from a relatively loose to a tight state in the presence of caffeic acid.Overall,these findings are meaningful for screening bioactive compounds from various food-derived plant tissues using a combination of practical experimentation and molecular simulations.
