Genome editing approaches,particularly the CRISPR/Cas9 technology,are becoming state-of-the-art for trait development in numerous breeding programs.Significant advances in improving plant traits are enabled by this in...Genome editing approaches,particularly the CRISPR/Cas9 technology,are becoming state-of-the-art for trait development in numerous breeding programs.Significant advances in improving plant traits are enabled by this influential tool,especially for disease resistance,compared to traditional breeding.One of the potyviruses,the turnip mosaic virus(TuMV),is the most widespread and damaging virus that infects Brassica spp.worldwide.We generated the targeted mutation at the eIF(iso)4E gene in the TuMV-susceptible cultivar“Seoul”using CRISPR/Cas9 to develop TuMV-resistant Chinese cabbage.We detected several heritable indel mutations in the edited T0 plants and developed T1 through generational progression.It was indicated in the sequence analysis of the eIF(iso)4E-edited T1 plants that the mutations were transferred to succeeding generations.These edited T1 plants conferred resistance to TuMV.It was shown with ELISA analysis the lack of accumulation of viral particles.Furthermore,we found a strong negative correlation(r=-0.938)between TuMV resistance and the genome editing frequency of eIF(iso)4E.Consequently,it was revealed in this study that CRISPR/Cas9 technique can expedite the breeding process to improve traits in Chinese cabbage plants.展开更多
基金This work was carried out with the support of“Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ01652201)”Rural Development Administration,Republic of Korea.
文摘Genome editing approaches,particularly the CRISPR/Cas9 technology,are becoming state-of-the-art for trait development in numerous breeding programs.Significant advances in improving plant traits are enabled by this influential tool,especially for disease resistance,compared to traditional breeding.One of the potyviruses,the turnip mosaic virus(TuMV),is the most widespread and damaging virus that infects Brassica spp.worldwide.We generated the targeted mutation at the eIF(iso)4E gene in the TuMV-susceptible cultivar“Seoul”using CRISPR/Cas9 to develop TuMV-resistant Chinese cabbage.We detected several heritable indel mutations in the edited T0 plants and developed T1 through generational progression.It was indicated in the sequence analysis of the eIF(iso)4E-edited T1 plants that the mutations were transferred to succeeding generations.These edited T1 plants conferred resistance to TuMV.It was shown with ELISA analysis the lack of accumulation of viral particles.Furthermore,we found a strong negative correlation(r=-0.938)between TuMV resistance and the genome editing frequency of eIF(iso)4E.Consequently,it was revealed in this study that CRISPR/Cas9 technique can expedite the breeding process to improve traits in Chinese cabbage plants.
