The assembly of the three-dimensional(3D) lanthanide complexes,aiming at obtaining large magnetocalo ric effects,encounte rs a substantial challenge.In this study,we successfully isolated a novel series of Lnexclusive...The assembly of the three-dimensional(3D) lanthanide complexes,aiming at obtaining large magnetocalo ric effects,encounte rs a substantial challenge.In this study,we successfully isolated a novel series of Lnexclusive 3D complexes,fo rmulated as {[Ln_(2)(EDTA)(C_(2)O_(4))(H_(2)O)_(2)]}n(abbreviated as Ln_(2),Ln=Gd^(Ⅲ)(1),Eu^(Ⅲ)(2),Sm^(Ⅲ)(3),H_(4)EDTA=ethylene diamine tetraacetic acid;H_(2)C_(2)O_(4)=oxalic acid).Crystallographic study exhibits that complex 1 features a cute snail-shaped Gd_(2) unit.Adjacent Gd_(2) units are aggregated by hexadentate EDTA^(4-) and C_(2)O_(4)^(2-)ligands,further constructing a charming three-dimensional metal-organic framework with interesting parallelogram-shaped layers.Notably,all coordinated EDTA^(4-)ligands and lightweight C_(2)O_(4)^(2-)groups contribute to building a densely packed metal-organic framework,endowing complex 1 with remarkable magnetocaloric effect(-ΔS_(m)^(max)=42.5 J/(kg·K) at 2.5 K and ΔH=7.0 T).Additionally,complexes 2 and 3 exhibit outstanding solid-state luminescent properties with lifetimes of43 8.22 and 4.13 μs,and quantum yields(QY) of 7.03% and 15.46%,respectively.展开更多
Chronic hepatitis B(CHB)infection remains a serious public health problem worldwide;however,the relationship between cholesterol levels and CHB remains unclear.We isolated peripheral blood mononuclear cells from healt...Chronic hepatitis B(CHB)infection remains a serious public health problem worldwide;however,the relationship between cholesterol levels and CHB remains unclear.We isolated peripheral blood mononuclear cells from healthy blood donors and CHB patients to analyze free cholesterol levels,lipid raft formation,and cholesterol metabolism-related pathways.Hepatitis B virus(HBV)-carrier mice were generated and used to confirm changes in cholesterol metabolism and cell-surface lipid raft formation in dendritic cells(DCs)in the context of CHB.Additionally,HBV-carrier mice were immunized with a recombinant HBV vaccine(rHBVvac)combined with lipophilic statins and evaluated for vaccine efficacy against HBV.Serum samples were analyzed for HBsAg,anti-HBs,and alanine aminotransferase levels,and liver samples were evaluated for HBV DNA and RNA and HBcAg.CHB reduced free cholesterol levels and suppressed lipid raft formation on DCs in patients with CHB and HBV-carrier mice,whereas administration of lipophilic statins promoted free cholesterol accumulation and restored lipid rafts on DCs accompanied by an enhanced antigen-presentation ability in vitro and in vivo.Cholesterol accumulation on DCs improved the rHBVvac-mediated elimination of serum HBV DNA and intrahepatic HBV DNA,HBV RNA,and HBcAg and promoted the rHBVvac-mediated generation and polyfunctionality of HBV-specific CD11a^(hi) CD8α^(lo) cells,induction of the development of memory responses against HBV reinfection,and seroconversion from HBsAg to anti-HBs.The results demonstrated the important role of cholesterol levels in DC dysfunction during CHB,suggesting that strategies to increase cholesterol accumulation on DCs might enhance therapeutic vaccine efficacy against HBV and support development toward clinical CHB treatment.展开更多
基金Project supported by the National Natural Science Foundation of China (92161109)the Postgraduate Research&Practice Innovation Program of Jiangsu Province (KYCX24_1566)。
文摘The assembly of the three-dimensional(3D) lanthanide complexes,aiming at obtaining large magnetocalo ric effects,encounte rs a substantial challenge.In this study,we successfully isolated a novel series of Lnexclusive 3D complexes,fo rmulated as {[Ln_(2)(EDTA)(C_(2)O_(4))(H_(2)O)_(2)]}n(abbreviated as Ln_(2),Ln=Gd^(Ⅲ)(1),Eu^(Ⅲ)(2),Sm^(Ⅲ)(3),H_(4)EDTA=ethylene diamine tetraacetic acid;H_(2)C_(2)O_(4)=oxalic acid).Crystallographic study exhibits that complex 1 features a cute snail-shaped Gd_(2) unit.Adjacent Gd_(2) units are aggregated by hexadentate EDTA^(4-) and C_(2)O_(4)^(2-)ligands,further constructing a charming three-dimensional metal-organic framework with interesting parallelogram-shaped layers.Notably,all coordinated EDTA^(4-)ligands and lightweight C_(2)O_(4)^(2-)groups contribute to building a densely packed metal-organic framework,endowing complex 1 with remarkable magnetocaloric effect(-ΔS_(m)^(max)=42.5 J/(kg·K) at 2.5 K and ΔH=7.0 T).Additionally,complexes 2 and 3 exhibit outstanding solid-state luminescent properties with lifetimes of43 8.22 and 4.13 μs,and quantum yields(QY) of 7.03% and 15.46%,respectively.
基金supported by the National Key Research and Development Programme (2021YFC2300603)the National Postdoctoral Programme for Innovative Talents (No.BX20190192)+1 种基金the China Postdoctoral Science Foundation (No.2020M672064)the National Science Foundation for Young Scientists of China (No.82001687).
文摘Chronic hepatitis B(CHB)infection remains a serious public health problem worldwide;however,the relationship between cholesterol levels and CHB remains unclear.We isolated peripheral blood mononuclear cells from healthy blood donors and CHB patients to analyze free cholesterol levels,lipid raft formation,and cholesterol metabolism-related pathways.Hepatitis B virus(HBV)-carrier mice were generated and used to confirm changes in cholesterol metabolism and cell-surface lipid raft formation in dendritic cells(DCs)in the context of CHB.Additionally,HBV-carrier mice were immunized with a recombinant HBV vaccine(rHBVvac)combined with lipophilic statins and evaluated for vaccine efficacy against HBV.Serum samples were analyzed for HBsAg,anti-HBs,and alanine aminotransferase levels,and liver samples were evaluated for HBV DNA and RNA and HBcAg.CHB reduced free cholesterol levels and suppressed lipid raft formation on DCs in patients with CHB and HBV-carrier mice,whereas administration of lipophilic statins promoted free cholesterol accumulation and restored lipid rafts on DCs accompanied by an enhanced antigen-presentation ability in vitro and in vivo.Cholesterol accumulation on DCs improved the rHBVvac-mediated elimination of serum HBV DNA and intrahepatic HBV DNA,HBV RNA,and HBcAg and promoted the rHBVvac-mediated generation and polyfunctionality of HBV-specific CD11a^(hi) CD8α^(lo) cells,induction of the development of memory responses against HBV reinfection,and seroconversion from HBsAg to anti-HBs.The results demonstrated the important role of cholesterol levels in DC dysfunction during CHB,suggesting that strategies to increase cholesterol accumulation on DCs might enhance therapeutic vaccine efficacy against HBV and support development toward clinical CHB treatment.
