Background:Hepatocellular carcinoma(HCC)is a major cause of cancer-related deaths.The Nod-like receptor(NLR)family is involved in innate immunity and tumor progression,but its role inHCC remains unclear.This study aim...Background:Hepatocellular carcinoma(HCC)is a major cause of cancer-related deaths.The Nod-like receptor(NLR)family is involved in innate immunity and tumor progression,but its role inHCC remains unclear.This study aimed to evaluate the prognostic value and biological function of NLR genes in HCC.Methods:Transcriptomic and clinical data from The Cancer Genome Atlas were analyzed using nonnegative matrix factorization(NMF)to classify HCC into molecular subtypes.Differentially expressed genes were used to build an NLR-based prognostic model(NLR_score)through univariate Cox,least absolute shrinkage and selection operator(LASSO),andmultivariate Cox regression.Predictive performance and correlation with chemotherapy sensitivity were assessed.NLR family pyrin domain containing 5(NLRP5)was identified as a key oncogene and validated via in vitro assays,including cell counting kit-8(CCK-8),colony formation,transwell,and flow cytometry in vivo xenograft models.Results:The two NMFdefined subtypes showed distinct survival outcomes.The NLR_score reliably predicted prognosis and was associated with sensitivity to six chemotherapeutic drugs.NLRP5 knockdown suppressed HCC cell proliferation,migration,and invasion in vitro and reduced tumor growth in vivo.Mechanistically,NLRP5 modulated the p53 signaling pathway,influencing cell cycle and apoptosis.Conclusion:This study developed an NLR-based prognosticmodel that effectively stratifies HCC patients by survival risk.NLRP5 was identified as a novel oncogene promoting HCC progression via the p53 pathway,suggesting its potential as a therapeutic target.展开更多
To shorten the existing box culvert demolition construction period and ensure the normal operation of the railway, the jacking-out construction method was adopted. The ABAQUS finite element software was used to establ...To shorten the existing box culvert demolition construction period and ensure the normal operation of the railway, the jacking-out construction method was adopted. The ABAQUS finite element software was used to establish a three-dimensional model of the box culvert and soil body of the relying project, and three excavation thickness (0m, 1 m, 2 m) were used as the main variation parameters for numerical analysis and research, and the change law of the box culvert itself and soil body stress during the culvert jacking out process was obtained. The results show that the jacking force-displacement curves of the three working conditions can be divided into two stages, and the jacking force reaches the maximum value at the moment when the static friction turns into sliding friction at the end of the first stage. The stress distribution at the bottom slab of the box culvert in the jacking process is approximately normal, and the stress decreases with the increase of the roadbed excavation thickness. The increase of the roadbed excavation thickness can reduce the soil pressure on the side of the box culvert and effectively reduce the deformation of the roadbed in the jacking-out process. The deformation of the roadbed during the jacking process can be reduced by increasing the thickness of the roadbed excavation.展开更多
Yield loss(Y_(Loss)) in the ratoon crop due to crushing damage to left stubble from mechanical harvesting of the main crop is a constraint for wide adoption of mechanized rice ratooning technology.Soil drying before t...Yield loss(Y_(Loss)) in the ratoon crop due to crushing damage to left stubble from mechanical harvesting of the main crop is a constraint for wide adoption of mechanized rice ratooning technology.Soil drying before the harvest of the main crop has been proposed to overcome this problem.The objective of this study was to determine the effect of soil drying during the mid-to-late grain filling stage of the main crop on grain yield of the ratoon crop in a mechanized rice ratooning system.Field experiments were conducted to compare Y_(Loss) between light(LD) and heavy(HD) soil drying treatments in Hubei province,central China in 2017 and 2018.Y_(Loss) was calculated as the percentage of yield reduction in the ratoon crop with the main crop harvested mechanically,relative to the grain yield of the ratoon crop with the main crop harvested manually.In comparison with LD,soil hardness was increased by 42.8%-84.7% in HD at the 5-20 cm soil depth at maturity of the main crop.Soil hardness at 5 and 10 cm depths reached respectively 4.05 and 7.07 kg cm^(-2) in HD.Soil drying treatment did not significantly affect the grain yield of the main crop.Under mechanical harvesting of the main crop,HD increased the grain yield of the ratoon crop by 9.4% relative to LD.Consequently,Y_(Loss) was only 3.4% in HD,in contrast to 16.3% in LD.The differences in grain yield and Y_(Loos) between the two soil drying treatments were explained mainly by panicles m^(-2),which was increased significantly by HD in the track zone of the ratoon crop compared with LD.These results suggest that heavy soil drying practice during the mid-to-late grain filling stage of the main crop is effective for reducing Y_(Loss) of the ratoon crop in a mechanized rice ratooning system.展开更多
Summary What is already known about this topic?Trainees in the China Field Epidemiology Training Program(CFETP)constitute a vital workforce in addressing global public health emergencies.Developing intercultural commu...Summary What is already known about this topic?Trainees in the China Field Epidemiology Training Program(CFETP)constitute a vital workforce in addressing global public health emergencies.Developing intercultural communication competence is essential for their future participation in international public health efforts.However,within China’s existing public health training system,this aspect has not yet received adequate attention or been systematically strengthened.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81973983,82270015,82100017,82302577,82304209,82370016)Anhui Province Scientific Research Planning Project(2023AH010083,2023AH053282)+4 种基金the Joint Construction Project of Clinical Medicine University and Hospital(No.2021lcxk006)the Anhui Provincial Natural Science Foundation(Nos.2208085MH264,2308085QH284,2308085MH243)the China Primary Health Care Foundation(No.MTP2022A015)the Project Supported by Anhui Medical University(2021xkj138,2021xkj067)Anhui Provincial Health Research Project(AHWJ2022b076).
文摘Background:Hepatocellular carcinoma(HCC)is a major cause of cancer-related deaths.The Nod-like receptor(NLR)family is involved in innate immunity and tumor progression,but its role inHCC remains unclear.This study aimed to evaluate the prognostic value and biological function of NLR genes in HCC.Methods:Transcriptomic and clinical data from The Cancer Genome Atlas were analyzed using nonnegative matrix factorization(NMF)to classify HCC into molecular subtypes.Differentially expressed genes were used to build an NLR-based prognostic model(NLR_score)through univariate Cox,least absolute shrinkage and selection operator(LASSO),andmultivariate Cox regression.Predictive performance and correlation with chemotherapy sensitivity were assessed.NLR family pyrin domain containing 5(NLRP5)was identified as a key oncogene and validated via in vitro assays,including cell counting kit-8(CCK-8),colony formation,transwell,and flow cytometry in vivo xenograft models.Results:The two NMFdefined subtypes showed distinct survival outcomes.The NLR_score reliably predicted prognosis and was associated with sensitivity to six chemotherapeutic drugs.NLRP5 knockdown suppressed HCC cell proliferation,migration,and invasion in vitro and reduced tumor growth in vivo.Mechanistically,NLRP5 modulated the p53 signaling pathway,influencing cell cycle and apoptosis.Conclusion:This study developed an NLR-based prognosticmodel that effectively stratifies HCC patients by survival risk.NLRP5 was identified as a novel oncogene promoting HCC progression via the p53 pathway,suggesting its potential as a therapeutic target.
文摘To shorten the existing box culvert demolition construction period and ensure the normal operation of the railway, the jacking-out construction method was adopted. The ABAQUS finite element software was used to establish a three-dimensional model of the box culvert and soil body of the relying project, and three excavation thickness (0m, 1 m, 2 m) were used as the main variation parameters for numerical analysis and research, and the change law of the box culvert itself and soil body stress during the culvert jacking out process was obtained. The results show that the jacking force-displacement curves of the three working conditions can be divided into two stages, and the jacking force reaches the maximum value at the moment when the static friction turns into sliding friction at the end of the first stage. The stress distribution at the bottom slab of the box culvert in the jacking process is approximately normal, and the stress decreases with the increase of the roadbed excavation thickness. The increase of the roadbed excavation thickness can reduce the soil pressure on the side of the box culvert and effectively reduce the deformation of the roadbed in the jacking-out process. The deformation of the roadbed during the jacking process can be reduced by increasing the thickness of the roadbed excavation.
基金supported by the Major International (Regional)Joint Research Project of National Natural Science Foundation of China (32061143038)the China Agriculture Research System(CARS-01-20)the Fundamental Research Funds for the Central Universities (2662020ZKPY015)。
文摘Yield loss(Y_(Loss)) in the ratoon crop due to crushing damage to left stubble from mechanical harvesting of the main crop is a constraint for wide adoption of mechanized rice ratooning technology.Soil drying before the harvest of the main crop has been proposed to overcome this problem.The objective of this study was to determine the effect of soil drying during the mid-to-late grain filling stage of the main crop on grain yield of the ratoon crop in a mechanized rice ratooning system.Field experiments were conducted to compare Y_(Loss) between light(LD) and heavy(HD) soil drying treatments in Hubei province,central China in 2017 and 2018.Y_(Loss) was calculated as the percentage of yield reduction in the ratoon crop with the main crop harvested mechanically,relative to the grain yield of the ratoon crop with the main crop harvested manually.In comparison with LD,soil hardness was increased by 42.8%-84.7% in HD at the 5-20 cm soil depth at maturity of the main crop.Soil hardness at 5 and 10 cm depths reached respectively 4.05 and 7.07 kg cm^(-2) in HD.Soil drying treatment did not significantly affect the grain yield of the main crop.Under mechanical harvesting of the main crop,HD increased the grain yield of the ratoon crop by 9.4% relative to LD.Consequently,Y_(Loss) was only 3.4% in HD,in contrast to 16.3% in LD.The differences in grain yield and Y_(Loos) between the two soil drying treatments were explained mainly by panicles m^(-2),which was increased significantly by HD in the track zone of the ratoon crop compared with LD.These results suggest that heavy soil drying practice during the mid-to-late grain filling stage of the main crop is effective for reducing Y_(Loss) of the ratoon crop in a mechanized rice ratooning system.
基金Supported by funds from African countries to enhance their public health capacitybuilding projects[No.OPP1161303(GAT/16/303)].
文摘Summary What is already known about this topic?Trainees in the China Field Epidemiology Training Program(CFETP)constitute a vital workforce in addressing global public health emergencies.Developing intercultural communication competence is essential for their future participation in international public health efforts.However,within China’s existing public health training system,this aspect has not yet received adequate attention or been systematically strengthened.
