A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum a...A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum .展开更多
基金the National Natural Science Foundation of China (Accession no. 81573586, 90612002).
文摘A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum .
