Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study ...Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study investigated the presence of Notchl, hairy and enhancer of split-l, and glial fibrillary acidic protein in the temporal neocortex and hippocampus of lithium-pilocar- pine-treated rats. The presence of Notchl and hairy and enhancer of split-1 was also explored in brain tissues of patients with intractable temporal lobe epilepsy. Quantitative electroencephalo- gram analysis and behavioral observations were used as auxiliary measures. Results revealed that the presence of Notchl, hairy and enhancer of split-l, and glial fibriUary acidic protein were en- hanced in status epilepticus and vehicle-treated spontaneous recurrent seizures rats, but remain unchanged in the following groups： control, absence of either status epilepticus or spontaneous recurrent seizures, and zileuton-treated spontaneous recurrent seizures. Compared with patient control cases, the presences of Notch1 and hairy and enhancer of split- 1 were upregulated in the temporal neocortex of patients with intractable temporal lobe epilepsy. Therefore, these results suggest that Notchl signaling may play an important role in the onset of temporal lobe epilepsy via astrogliosis. Furthermore, zileuton may be a potential therapeutic strategy for temporal lobe epilepsy by blocking Notchl signaling.展开更多
Gray mold,caused by the fungal pathogen Botrytis cinerea,is a major threat to global agriculture.Conventional pesticide control exacerbates environmental pollution and increases the risk of resistance.Although RNA int...Gray mold,caused by the fungal pathogen Botrytis cinerea,is a major threat to global agriculture.Conventional pesticide control exacerbates environmental pollution and increases the risk of resistance.Although RNA interference(RNAi)holds potential for disease management,its application is limited by RNA instability and ambiguous targeting.Here,we demonstrate that tomato microRNA393(miR393)can move across kingdoms into B.cinerea and reduce its pathogenicity.Using RT–qPCR,GUS staining,and 5′RLM–RACE,we confirmed that miR393 specifically targets nucleotides 1777–1778 of the BcFKS1 gene in B.cinerea,thereby suppressing its expression and disrupting fungal cell wall integrity.To protect miR393 from degradation,we developed a star polycation(SPc)–based nanocarrier delivery system.This nanomaterial can extend the degradation half-life of miR393 six-fold under RNase A and RNase III treatment(up to 2 h)and increase delivery efficiency by 35%–65%.SPc-loaded miR393 enhances tomato immunity by activating the jasmonic acid and salicylic acid pathways,suppressing auxin receptor genes,and inducing PR1 protein accumulation(6.8-fold).Inoculation assays further showed that SPc-loaded double-stranded miR393 can reduce gray mold lesion areas by 67%in tomato,88%in strawberry,and 41%in grape.This study elucidates the dual mechanism by which miR393 controls gray mold—cross-kingdom regulation of pathogenic fungi and modulation of plant immunity—and provides a promising strategy for sustainable disease prevention and control.展开更多
基金funded by the Natural Science Foundation of Hubei Province in China,No.02.02.040458
文摘Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study investigated the presence of Notchl, hairy and enhancer of split-l, and glial fibrillary acidic protein in the temporal neocortex and hippocampus of lithium-pilocar- pine-treated rats. The presence of Notchl and hairy and enhancer of split-1 was also explored in brain tissues of patients with intractable temporal lobe epilepsy. Quantitative electroencephalo- gram analysis and behavioral observations were used as auxiliary measures. Results revealed that the presence of Notchl, hairy and enhancer of split-l, and glial fibriUary acidic protein were en- hanced in status epilepticus and vehicle-treated spontaneous recurrent seizures rats, but remain unchanged in the following groups： control, absence of either status epilepticus or spontaneous recurrent seizures, and zileuton-treated spontaneous recurrent seizures. Compared with patient control cases, the presences of Notch1 and hairy and enhancer of split- 1 were upregulated in the temporal neocortex of patients with intractable temporal lobe epilepsy. Therefore, these results suggest that Notchl signaling may play an important role in the onset of temporal lobe epilepsy via astrogliosis. Furthermore, zileuton may be a potential therapeutic strategy for temporal lobe epilepsy by blocking Notchl signaling.
基金supported by the Innovational Fund for Scientific and Technological Personnel of Hainan Province(KJRC2023C42)the Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture,Hainan University(XTCX2022NYB12).
文摘Gray mold,caused by the fungal pathogen Botrytis cinerea,is a major threat to global agriculture.Conventional pesticide control exacerbates environmental pollution and increases the risk of resistance.Although RNA interference(RNAi)holds potential for disease management,its application is limited by RNA instability and ambiguous targeting.Here,we demonstrate that tomato microRNA393(miR393)can move across kingdoms into B.cinerea and reduce its pathogenicity.Using RT–qPCR,GUS staining,and 5′RLM–RACE,we confirmed that miR393 specifically targets nucleotides 1777–1778 of the BcFKS1 gene in B.cinerea,thereby suppressing its expression and disrupting fungal cell wall integrity.To protect miR393 from degradation,we developed a star polycation(SPc)–based nanocarrier delivery system.This nanomaterial can extend the degradation half-life of miR393 six-fold under RNase A and RNase III treatment(up to 2 h)and increase delivery efficiency by 35%–65%.SPc-loaded miR393 enhances tomato immunity by activating the jasmonic acid and salicylic acid pathways,suppressing auxin receptor genes,and inducing PR1 protein accumulation(6.8-fold).Inoculation assays further showed that SPc-loaded double-stranded miR393 can reduce gray mold lesion areas by 67%in tomato,88%in strawberry,and 41%in grape.This study elucidates the dual mechanism by which miR393 controls gray mold—cross-kingdom regulation of pathogenic fungi and modulation of plant immunity—and provides a promising strategy for sustainable disease prevention and control.
