Grain boundary engineering plays a significant role in the improvement of strength and plasticity of alloys. However, in refractory high-entropy alloys, the susceptibility of grain boundaries to oxygen presents a bott...Grain boundary engineering plays a significant role in the improvement of strength and plasticity of alloys. However, in refractory high-entropy alloys, the susceptibility of grain boundaries to oxygen presents a bottleneck in achieving high mechanical performance. Creating a large number of clean grain boundaries in refractory high-entropy alloys is a challenge. In this study, an ultrafine-grained (UFG) NbMoTaW alloy with high grain-boundary cohesion was prepared by powder metallurgy, taking advantages of rapid hot-pressing sintering and full-process inert atmosphere protection from powder synthesis to sintering. By oxygen control and an increase in the proportion of grain boundaries, the segregation of oxygen and formation of oxides at grain boundaries were strongly mitigated, thus the intrinsic high cohesion of the interfaces was preserved. Compared to the coarse-grained alloys prepared by arc-melting and those sintered by traditional powder metallurgy methods, the UFG NbMoTaW alloy demonstrated simultaneously increased strength and plasticity at ambient temperature. The highly cohesive grain boundaries not only reduce brittle fractures effectively but also promote intragranular deformation. Consequently, the UFG NbMoTaW alloy achieved a high yield strength even at elevated temperatures, with a remarkable performance of 1117 MPa at 1200 ℃. This work provides a feasible solution for producing refractory high-entropy alloys with low impurity content, refined microstructure, and excellent mechanical performance.展开更多
Background: Our study aimed to investigate the clinical value of platelet parameters and the number of Monocytes (MONO) in the peripheral blood of patients with Ocular Myasthenia Gravis (OMG). Methods: Fifty patients ...Background: Our study aimed to investigate the clinical value of platelet parameters and the number of Monocytes (MONO) in the peripheral blood of patients with Ocular Myasthenia Gravis (OMG). Methods: Fifty patients discharged from the Department of Neurology of the First People’s Hospital of Jingzhou City with the diagnosis of OMG from January 1, 2020, to December 31, 2023, were selected as the experimental group, and healthy medical examiners were selected as the control group during the same period. The differences in peripheral blood mononuclear cell count and platelet-related parameters between the two groups were compared. ROC curves were drawn to analyze the clinical value of the studied indexes in patients with OMG. Results: Patients with OMG had significantly higher monocyte counts and narrower platelet distributions compared with normal adults;further logistic regression analysis revealed that higher monocyte counts and narrower Platelet Distribution Width (PDW) may be independent influences on the occurrence of OMG (p Conclusion: Peripheral blood mononuclear cell count and PDW have some diagnostic value in OMG.展开更多
In recent decades, the treatment of myasthenia gravis has been extensively developed, but a standardized standard still needs to be used. Its treatment strategy is associated with patient prognosis, economic costs, an...In recent decades, the treatment of myasthenia gravis has been extensively developed, but a standardized standard still needs to be used. Its treatment strategy is associated with patient prognosis, economic costs, and complications. This article reviews the pathogenesis, treatment methods, and complications of myasthenia gravis, providing new ideas for diagnosing and treating myasthenia gravis and fully embodies the principle of safety and precision.展开更多
Objective: To investigate the feasibility, safety and the clinical value of primary suture following 3-port laparoscopic common bile duct exploration (LCBDE). Methods: From January 2012 to September 2014, 176 patients...Objective: To investigate the feasibility, safety and the clinical value of primary suture following 3-port laparoscopic common bile duct exploration (LCBDE). Methods: From January 2012 to September 2014, 176 patients suffered from choledocholithiasis were treated with primary suture following 3-port LCBDE and the clinical data were retrospectively analyzed. Results: All cases were operated successfully and none was converted to open surgery. The duration of operation was 92.2 ± 18.8 min and the length of postoperative hospital stay was 4.4 ± 3.7 d. Postoperative bile leakage occurred in 2 cases and these patients recovered by simple drainage for 3 to 7 days without re-operation. All patients recovered smoothly without any serious complications. Conclusions: Primary suture following 3-port LCBDE is safe, effective and mini-invasive, which is worthy of further clinical application.展开更多
The rapid advancement of biomedical polymers has raised significant concerns about the disposal of medical polymer waste.Sustainable biomass materials derived from renewable sources in na-ture have emerged as promisin...The rapid advancement of biomedical polymers has raised significant concerns about the disposal of medical polymer waste.Sustainable biomass materials derived from renewable sources in na-ture have emerged as promising alternatives to petroleum-based polymers for medical applica-tions and tissue engineering due to their abundance,biodegradability,and environmental friend-liness.In tissue engineering,interconnected macropores within biomaterials are crucial as they provide space and interfaces for cells,enhancing permeability for nutrient and waste transport.In this review,we summarize recent developments in the use of biomass materials to engineer macroporous tissue engineering scaffolds.We highlight key techniques,such as microparticles assembly,leaching template,and bioprinting that can create macropores within scaffolds com-posed of biomass materials and their composites.In addition,we investigate the applications of the macroporous scaffolds in wound healing,with a focus on cell behaviors within macroporous constructs and their role in treating chronic wounds.We envision that the combination of the bicontinuous macropores and biomass-based materials can create an ideal cellular environment and provide a powerful platform for wound healing and tissue regeneration.展开更多
In the process of signal transmission,an inevitable phenomenon is that the signal may change abruptly at certain discrete moments,negatively contributing to the performance of the system.In this paper,we focus on the ...In the process of signal transmission,an inevitable phenomenon is that the signal may change abruptly at certain discrete moments,negatively contributing to the performance of the system.In this paper,we focus on the distributed state estimation problem for continuous-time linear time-invariant(LTI)systems with intermittent abrupt jumps in communication information.First,the phenomenon of intermittent sudden jumps during information transmission is characterized as impulsive communication disturbances.Under impulsive communication disturbances,a distributed reduced order observer(DROO)is designed with each local observer grouped with two modules:a reduced-order observer and a consensus-based observer.Then,sufficient conditions for the existence of the DROO are presented,by using the graph theory and impulsive analysis.The relationship between the feedback gain and the unstable system matrix,the network topology,the impulse intensity,and the average impulsive interval is obtained.Finally,the validity of the proposed DROO under impulsive communication disturbances is substantiated by numerical simulations.展开更多
In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples.Although its multiplexing capacity a...In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples.Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results.Here,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method.We also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics experiments.In the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics.展开更多
Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is unclea...Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is unclear.In addition,previous studies have shown that eicosapentaenoic acid(EPA),aω-3 polyunsaturated fatty acid(PUFA),reverses PA-induced muscle IR,but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further clarified.Here,we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes in HepG2 cells after treatment with different free fatty acids(FFAs).A total of 234 proteins were determined to be differentially expressed after PA+OA treatment.Their functions were mainly related to responses to stress and endogenous stimuli,lipid metabolic process,and protein binding.For PA+EPA treatment,the PA-induced expression changes of 1326 proteins could be reversed by EPA,415 of which were mitochondrial proteins,with most of the functional proteins involved in oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)cycle.Mechanistic studies revealed that the protein encoded by JUN and reactive oxygen species(ROS)play a role in OA-and EPA-reversed PA-induced IR,respectively.EPA and OA alleviated PA-induced abnormal adenosine triphosphate(ATP)production,ROS generation,and calcium(Ca^(2+))content.Importantly,H_(2)O_(2)-activated production of ROS increased the protein expression of JUN,further resulting in IR in HepG2 cells.Taken together,we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52371128,52304378,52101031 and 92163107).
文摘Grain boundary engineering plays a significant role in the improvement of strength and plasticity of alloys. However, in refractory high-entropy alloys, the susceptibility of grain boundaries to oxygen presents a bottleneck in achieving high mechanical performance. Creating a large number of clean grain boundaries in refractory high-entropy alloys is a challenge. In this study, an ultrafine-grained (UFG) NbMoTaW alloy with high grain-boundary cohesion was prepared by powder metallurgy, taking advantages of rapid hot-pressing sintering and full-process inert atmosphere protection from powder synthesis to sintering. By oxygen control and an increase in the proportion of grain boundaries, the segregation of oxygen and formation of oxides at grain boundaries were strongly mitigated, thus the intrinsic high cohesion of the interfaces was preserved. Compared to the coarse-grained alloys prepared by arc-melting and those sintered by traditional powder metallurgy methods, the UFG NbMoTaW alloy demonstrated simultaneously increased strength and plasticity at ambient temperature. The highly cohesive grain boundaries not only reduce brittle fractures effectively but also promote intragranular deformation. Consequently, the UFG NbMoTaW alloy achieved a high yield strength even at elevated temperatures, with a remarkable performance of 1117 MPa at 1200 ℃. This work provides a feasible solution for producing refractory high-entropy alloys with low impurity content, refined microstructure, and excellent mechanical performance.
文摘Background: Our study aimed to investigate the clinical value of platelet parameters and the number of Monocytes (MONO) in the peripheral blood of patients with Ocular Myasthenia Gravis (OMG). Methods: Fifty patients discharged from the Department of Neurology of the First People’s Hospital of Jingzhou City with the diagnosis of OMG from January 1, 2020, to December 31, 2023, were selected as the experimental group, and healthy medical examiners were selected as the control group during the same period. The differences in peripheral blood mononuclear cell count and platelet-related parameters between the two groups were compared. ROC curves were drawn to analyze the clinical value of the studied indexes in patients with OMG. Results: Patients with OMG had significantly higher monocyte counts and narrower platelet distributions compared with normal adults;further logistic regression analysis revealed that higher monocyte counts and narrower Platelet Distribution Width (PDW) may be independent influences on the occurrence of OMG (p Conclusion: Peripheral blood mononuclear cell count and PDW have some diagnostic value in OMG.
文摘In recent decades, the treatment of myasthenia gravis has been extensively developed, but a standardized standard still needs to be used. Its treatment strategy is associated with patient prognosis, economic costs, and complications. This article reviews the pathogenesis, treatment methods, and complications of myasthenia gravis, providing new ideas for diagnosing and treating myasthenia gravis and fully embodies the principle of safety and precision.
基金supported by the Henan University of Chinese Medicine Doctoral Research Initiation Fund Project(RSBSJJ2020-17)National Key R&D Program-Modernization of Chinese Medicine(2019YFC1708802).
文摘Objective: To investigate the feasibility, safety and the clinical value of primary suture following 3-port laparoscopic common bile duct exploration (LCBDE). Methods: From January 2012 to September 2014, 176 patients suffered from choledocholithiasis were treated with primary suture following 3-port LCBDE and the clinical data were retrospectively analyzed. Results: All cases were operated successfully and none was converted to open surgery. The duration of operation was 92.2 ± 18.8 min and the length of postoperative hospital stay was 4.4 ± 3.7 d. Postoperative bile leakage occurred in 2 cases and these patients recovered by simple drainage for 3 to 7 days without re-operation. All patients recovered smoothly without any serious complications. Conclusions: Primary suture following 3-port LCBDE is safe, effective and mini-invasive, which is worthy of further clinical application.
基金supported by a Merit Award from the Veterans Health Administration-Office of Research and Development(VA-ORD BLR&D Merit Award,BX-19-001).
文摘The rapid advancement of biomedical polymers has raised significant concerns about the disposal of medical polymer waste.Sustainable biomass materials derived from renewable sources in na-ture have emerged as promising alternatives to petroleum-based polymers for medical applica-tions and tissue engineering due to their abundance,biodegradability,and environmental friend-liness.In tissue engineering,interconnected macropores within biomaterials are crucial as they provide space and interfaces for cells,enhancing permeability for nutrient and waste transport.In this review,we summarize recent developments in the use of biomass materials to engineer macroporous tissue engineering scaffolds.We highlight key techniques,such as microparticles assembly,leaching template,and bioprinting that can create macropores within scaffolds com-posed of biomass materials and their composites.In addition,we investigate the applications of the macroporous scaffolds in wound healing,with a focus on cell behaviors within macroporous constructs and their role in treating chronic wounds.We envision that the combination of the bicontinuous macropores and biomass-based materials can create an ideal cellular environment and provide a powerful platform for wound healing and tissue regeneration.
基金supported in part by the National Natural Science Foundation of China under Grant Nos.62425602 and 62273159
文摘In the process of signal transmission,an inevitable phenomenon is that the signal may change abruptly at certain discrete moments,negatively contributing to the performance of the system.In this paper,we focus on the distributed state estimation problem for continuous-time linear time-invariant(LTI)systems with intermittent abrupt jumps in communication information.First,the phenomenon of intermittent sudden jumps during information transmission is characterized as impulsive communication disturbances.Under impulsive communication disturbances,a distributed reduced order observer(DROO)is designed with each local observer grouped with two modules:a reduced-order observer and a consensus-based observer.Then,sufficient conditions for the existence of the DROO are presented,by using the graph theory and impulsive analysis.The relationship between the feedback gain and the unstable system matrix,the network topology,the impulse intensity,and the average impulsive interval is obtained.Finally,the validity of the proposed DROO under impulsive communication disturbances is substantiated by numerical simulations.
基金supported by grants from the National Key R&D Program of China (Grant Nos. 2018YFA0507801 and 2018YFA0507103)the National Natural Science Foundation of China (Grant No. 31900925)the China Scholarship Council (CSC)
文摘In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples.Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results.Here,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method.We also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics experiments.In the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics.
基金supported by grants from the National Key R&D Program of China(Grant Nos.2018YFA0507801 and 2018YFA0507103)the National Natural Science Foundation of China(Grant Nos.91640112 and 21607170)the Strategic Priority Research Programs of the Chinese Academy of Sciences(Grant No.XDA12030202)。
文摘Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is unclear.In addition,previous studies have shown that eicosapentaenoic acid(EPA),aω-3 polyunsaturated fatty acid(PUFA),reverses PA-induced muscle IR,but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further clarified.Here,we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes in HepG2 cells after treatment with different free fatty acids(FFAs).A total of 234 proteins were determined to be differentially expressed after PA+OA treatment.Their functions were mainly related to responses to stress and endogenous stimuli,lipid metabolic process,and protein binding.For PA+EPA treatment,the PA-induced expression changes of 1326 proteins could be reversed by EPA,415 of which were mitochondrial proteins,with most of the functional proteins involved in oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)cycle.Mechanistic studies revealed that the protein encoded by JUN and reactive oxygen species(ROS)play a role in OA-and EPA-reversed PA-induced IR,respectively.EPA and OA alleviated PA-induced abnormal adenosine triphosphate(ATP)production,ROS generation,and calcium(Ca^(2+))content.Importantly,H_(2)O_(2)-activated production of ROS increased the protein expression of JUN,further resulting in IR in HepG2 cells.Taken together,we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR.
