The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransm...The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that [Ca2＋]i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5-10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.展开更多
This review aimed to summarize the relationship between rumen bacteria and production performance in dairy cows.The composition and metabolic functions of rumen bacteria significantly influenced production performance...This review aimed to summarize the relationship between rumen bacteria and production performance in dairy cows.The composition and metabolic functions of rumen bacteria significantly influenced production performance.However,previous studies lacked a systematic review of how variations in rumen bacterial composition correlate with production performance.This review described the differences in rumen bacterial composition among dairy cows with varying production performance,focusing on milk yield and composition,feed efficiency(FE),average daily gain(ADG)and methane production.Additionally,we explored the differences in rumen bacteria across different parities and testing time points.Through this systematic summary,we discussed the impact of rumen bacterial composition on dairy cow production performance and provided new insights and guidelines for devising targeted microbiome adjustment strategies.展开更多
基金supported by the National Natural Science Foundation of China,No.30972441,81202158
文摘The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that [Ca2＋]i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5-10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.
基金supported by grants from the National Key R&D Program of China(No.2023YFD1801100).
文摘This review aimed to summarize the relationship between rumen bacteria and production performance in dairy cows.The composition and metabolic functions of rumen bacteria significantly influenced production performance.However,previous studies lacked a systematic review of how variations in rumen bacterial composition correlate with production performance.This review described the differences in rumen bacterial composition among dairy cows with varying production performance,focusing on milk yield and composition,feed efficiency(FE),average daily gain(ADG)and methane production.Additionally,we explored the differences in rumen bacteria across different parities and testing time points.Through this systematic summary,we discussed the impact of rumen bacterial composition on dairy cow production performance and provided new insights and guidelines for devising targeted microbiome adjustment strategies.
