Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide...Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide in the renin-angiotensin system(RAS),on hypoxia-induced retinal neovascularization and its underlying mechanisms.In vivo,the C57BL/6J mice with oxygen-induced retinopathy(OIR)were injected intravitreally with alamandine(1.0µmol/kg per eye).In vitro,human retinal microvascular endothelial cells(HRMECs)were utilized to investigate the effects of alamandine(10µg/mL)on proliferation,apoptosis,migration,and tubular formation under vascular endothelial growth factor(VEGF)stimulation.Single-cell RNA sequencing(scRNA-seq)matrix data from the Gene Expression Omnibus(GEO)database and RAS-related genes from the Molecular Signatures Database(MSigDB)were sourced for subsequent analyses.By integrating scRNA-seq data across multiple species,we identified that RAS-associated endothelial cell populations were highly related to retinal neovascularization.The liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis revealed a significant decrease in alamandine levels in both the serum and retina of OIR mice compared to those in the control group.Next,alamandine ameliorated hypoxia-induced retinal pathological neovascularization and physiologic revascularization in OIR mice.In vitro,alamandine effectively mitigated VEGF-induced proliferation,scratch wound healing,and tube formation of HRMECs primarily by inhibiting the hypoxia-inducible factor-1α(HIF-1α)/VEGF pathway.Further,coincubation with D-Pro7(Mas-related G protein-coupled receptor D(MrgD)antagonist)hindered the beneficial impacts of alamandine on hypoxia-induced pathological angiogenesis both in vivo and in vitro.Our findings suggested that alamandine could mitigate retinal neovascularization by targeting the MrgD-mediated HIF-1α/VEGF pathway,providing a potential therapeutic agent for OIR prevention and treatment.展开更多
To guarantee the blue skies for the 2022 Winter Olympics held in Beijing and Zhangjiakou from February 4 to 20,Beijing and its surrounding areas adopted a series of emission control measures.This provides an opportuni...To guarantee the blue skies for the 2022 Winter Olympics held in Beijing and Zhangjiakou from February 4 to 20,Beijing and its surrounding areas adopted a series of emission control measures.This provides an opportunity to determine the impacts of large-scale temporary control measures on the air quality in Beijing during this special period.Here,we applied the WRF-CMAQ model to quantify the contributions of emission reduction measures and meteorological conditions.Results show that meteorological conditions in 2022 decreased PM_(2.5)in Beijing by 6.9 and 11.8μg/m^(3)relative to 2021 under the scenarios with and without emission reductions,respectively.Strict emission reduction measures implemented in Beijing and seven neighboring provinces resulted in an average decrease of 13.0μg/m^(3)(-41.2%)in PM_(2.5)in Beijing.Over the entire period,local emission reductions contributed more to good air quality in Beijing than nonlocal emission reductions.Under the emission reduction scenario,local,controlled regions,other regions,and boundary conditions contributed 47.7%,42.0%,5.3%,and 5.0%to the PM_(2.5)concentrations in Beijing,respectively.The results indicate that during the cleaning period with the air masses from the northwest,the abatements of PM_(2.5)were mainly caused by local emission reductions.However,during the potential pollution period with the air masses from the east-northeast and west-southwest,the abatements of PM_(2.5)were caused by both local and nonlocal emission reductions almost equally.This implies that regional coordinated prevention and control strategies need to be arranged scientifically and rationally when heavy pollution events are forecasted.展开更多
To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Deli...To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Delicious')fruit cuticular wax biosynthesis,the expression of genes related to fruit cuticular wax biosynthesis and ethylene biosynthesis and signaling,ethylene production rate,respiration rate,wax constituents and structure,and fruit quality were determined at harvest and during cold storage.The results showed that 1-MCP inhibited the expression levels of ethylene biosynthesis and signaling-related genes,decreased fruit ethylene production,and inhibited the expression of Md LACS1,Md CER6,Md CER4 and Md WSD1,which resulted in decreases in alcohols,acids,and esters content in fruit cuticular wax.1-MCP also reduced fruit dropping rate from 17.17%to 12%;maintained fruit firmness,soluble solids,titratable acidity during cold storage;showed about as one fifth in the total length and one third in the widest width of wax crack as that in control at harvest.In contrast,Ethephon produced the opposite effects.In conclusion,1-MCP inhibited fruit ethylene biosynthesis and signaling,and influenced fruit cuticular wax biosynthesis.Thus,the fruit cuticular wax constituents and structure was altered,and the fruit quality were maintained at harvest and during cold storage.The results provide a new technology for improving apple fruit harvest and postharvest quality by preharvest application of sprayable 1-MCP.展开更多
The new ＂4-to-1＂ school-enterprise cooperation model is constructed in combination with the automation program provided by Tianshi College. The new school-enterprise cooperation model is an effective way for Tianshi...The new ＂4-to-1＂ school-enterprise cooperation model is constructed in combination with the automation program provided by Tianshi College. The new school-enterprise cooperation model is an effective way for Tianshi College to train high-quality skilled personnel, an important carrier to deepen the production-study-research cooperation education, and also an important part to improve the work quality of personnel cultivation. In China, it is an urgent need of the current employment situation, but also an important strategy for Tianshi College to attain a lon^-term stable develot^ment. In the orocess of imolementation, a win-win situation has been achieved bv constantly deenening reform.展开更多
Hydrogen energy carrier produced by water electrolysis in alkaline electrolytes is rather meaningful and significant for global sustainability imperatives,while the highpH condition usually leads to a poor reversibili...Hydrogen energy carrier produced by water electrolysis in alkaline electrolytes is rather meaningful and significant for global sustainability imperatives,while the highpH condition usually leads to a poor reversibility of proton adsorption and desorption that significantly determines the hydrogen-generation activity in hydrogen evolution reaction(HER)process.Herein,we demonstrate a remarkable Ru-Mo solid-solution nanocrystal catalyst in alkaline HER process by a very simple but feasible pyrolysis and alkali leaching strategy.Benefiting from the pinning effect and local chemical-and electronic-structure regulations of Mo solute atoms,an ultra-low overpotential(17.3 mV)and an exceptional stability(>100 h)at the typical current density of 10 mA·cm^(−2)are achieved on the ultrasmall Ru-Mo solid-solution nanocrystal catalyst in 1.0 M KOH electrolyte.Density function theory(DFT)calculations gain an insight into the synergistic effect of neighboring Ru and Mo sites in alkaline HER process,where Mo solute atoms are beneficial for the adsorption and activation of water molecules for proton generation and accumulation due to their rich outermost 4d vacant orbitals,while the energy-favorable Ru sites are responsible for the fast deprotonation kinetics of hydrogen intermediates.Our work may provide an interesting route for the development of efficient and stable solid-solution alloy nanocrystals towards alkaline water electrolysis and beyond.展开更多
Efficient genotype-independent transformation and genome editing are highly desirable for plant biotechnology research and product development efforts.We have developed a novel approach to enable fast,high-throughput,...Efficient genotype-independent transformation and genome editing are highly desirable for plant biotechnology research and product development efforts.We have developed a novel approach to enable fast,high-throughput,and genotype-flexible Agrobacterium-mediated transformation using the important crop soybean as a test system.This new method is called GiFT(genotype-independent fast transformation)and involves only a few simple steps.The method uses germinated seeds as explants,and DNA delivery is achieved through Agrobacterium infection of wounded explants as in conventional in vitro-based methods.Following infection,the wounded explants are incubated in liquid medium with a sublethal level of selection and then transplanted directly into soil.The transplanted seedlings are then selected with herbicide spray for 3 weeks.The time required from initiation to fully established healthy T0 transgenic events is about 35 days.The GiFT method requires minimal in vitro manipulation or use of tissue culture media.Because the regeneration occurs in planta,the GiFT method is highly flexible with respect to genotype,which we demonstrate via successful transformation of elite germplasms from diverse genetic backgrounds.We also show that the soybean GiFT method can be applied to both conventional binary vectors and CRISPR-Cas12a vectors for genome editing applications.Analyses of T1 progeny demonstrate that the events have a high inheritance rate and can be used for genome engineering applications.By minimizing the need for tissue culture,the novel approach described here significantly improves operational efficiency while greatly reducing personnel and supply costs.It is the first industry-scale transformation method to utilize in planta selection in a major field crop.展开更多
Background:Perturbations in bone marrow mesenchymal stem cell(BMSC)differentiation play an important role in steroid-induced osteonecrosis of the femoral head(SONFH).At present,studies on SONFH concentrate upon the ba...Background:Perturbations in bone marrow mesenchymal stem cell(BMSC)differentiation play an important role in steroid-induced osteonecrosis of the femoral head(SONFH).At present,studies on SONFH concentrate upon the balance within BMSC osteogenic and adipogenic differentiation.However,BMSC apoptosis as well as proliferation are important prerequisites in their differentiation.The hedgehog(HH)signaling pathway regulates bone cell apoptosis.Baicalin(BA),a well-known compound in traditional Chinese medicine,can affect the proliferation and apoptosis of numerous cell types via HH signaling.However,the potential role and mechanisms of BA on BMSCs are unclear.Thus,we aimed to explore the role of BA in dexamethasone(Dex)-induced BMSC apoptosis in this study.Methods:Primary BMSCs were treated with 10-6 mol/L Dex alone or with 5.0μmol/L,10.0μmol/L,or 50.0μmol/L BA for 24 hours followed by co-treatment with 5.0μmol/L,10.0μmol/L,or 50.0μmol/L BA and 10-6 mol/L Dex.Cell viability was assayed through the Cell Counting Kit-8(CCK-8).Cell apoptosis was evaluated using Annexin V-fluorescein isothiocyanate/propidium iodide(PI)staining followed by flow cytometry.The imaging and counting,respectively,of Hochest 33342/PI-stained cells were used to assess the morphological characteristics and proportion of apoptotic cells.To quantify the apoptosis-related proteins(e.g.,apoptosis regulator BAX[Bax],B-cell lymphoma 2[Bcl-2],caspase-3,and cleaved caspase-3)and HH signaling pathway proteins,western blotting was used.A HH-signaling pathway inhibitor was used to demonstrate that BA exerts its anti-apoptotic effects via the HH signaling pathway.Results:The results of CCK-8,Hoechst 33342/PI-staining,and flow cytometry showed that BA did not significantly promote cell proliferation(CCK-8:0μmol/L,100%;2.5μmol/L,98.58%;5.0μmol/L,95.18%;10.0μmol/L,98.11%;50.0μmol/L,99.38%,F=2.33,P>0.05),but it did attenuate the effect of Dex on apoptosis(Hoechst 33342/PI-staining:Dex+50.0μmol/L BA,12.27%vs.Dex,39.27%,t=20.62;flow cytometry:Dex+50.0μmol/L BA,12.68%vs.Dex,37.43%,t=11.56;Both P<0.05).The results of western blotting analysis showed that BA reversed Dex-induced apoptosis by activating the HH signaling pathway,which down-regulated the expression of Bax,cleaved-caspase 3,and suppressor of fused(SUFU)while up-regulating Bcl-2,sonic hedgehog(SHH),and zinc finger protein GLI-1(GLI-1)expression(Bax/Bcl-2:Dex+50.0μmol/L BA,1.09 vs.Dex,2.76,t=35.12;cleaved caspase-3/caspase-3:Dex+50.0μmol/L BA,0.38 vs.Dex,0.73,t=10.62;SHH:Dex+50.0μmol/L BA,0.50 vs.Dex,0.12,t=34.01;SUFU:Dex+50.0μmol/L BA,0.75 vs.Dex,1.19,t=10.78;GLI-1:Dex+50.0μmol/L BA,0.40 vs.Dex,0.11,t=30.68.All P<0.05).Conclusions:BA antagonizes Dex-induced apoptosis of human BMSCs by activating the HH signaling pathway.It is a potential candidate for preventing SONFH.展开更多
基金supported by the National Natural Science Foundation of China(Nos.82200379 and 82300309)the Shanghai Sailing Program(No.22YF1443100)+1 种基金the Academy Talent Special Fund of The First Affiliated Hospital of Nanjing Medical University(Nos.YNRCQN0312 and MXJL202208)the Jiangsu Funding Program for Excellent Postdoctoral Talent(No.2023ZB592),China.
文摘Retinopathy of prematurity(ROP)is a vision-threatening disorder that leads to pathological growth of the retinal vasculature due to hypoxia.Here,we investigated the potential effects of alamandine,a novel heptapeptide in the renin-angiotensin system(RAS),on hypoxia-induced retinal neovascularization and its underlying mechanisms.In vivo,the C57BL/6J mice with oxygen-induced retinopathy(OIR)were injected intravitreally with alamandine(1.0µmol/kg per eye).In vitro,human retinal microvascular endothelial cells(HRMECs)were utilized to investigate the effects of alamandine(10µg/mL)on proliferation,apoptosis,migration,and tubular formation under vascular endothelial growth factor(VEGF)stimulation.Single-cell RNA sequencing(scRNA-seq)matrix data from the Gene Expression Omnibus(GEO)database and RAS-related genes from the Molecular Signatures Database(MSigDB)were sourced for subsequent analyses.By integrating scRNA-seq data across multiple species,we identified that RAS-associated endothelial cell populations were highly related to retinal neovascularization.The liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis revealed a significant decrease in alamandine levels in both the serum and retina of OIR mice compared to those in the control group.Next,alamandine ameliorated hypoxia-induced retinal pathological neovascularization and physiologic revascularization in OIR mice.In vitro,alamandine effectively mitigated VEGF-induced proliferation,scratch wound healing,and tube formation of HRMECs primarily by inhibiting the hypoxia-inducible factor-1α(HIF-1α)/VEGF pathway.Further,coincubation with D-Pro7(Mas-related G protein-coupled receptor D(MrgD)antagonist)hindered the beneficial impacts of alamandine on hypoxia-induced pathological angiogenesis both in vivo and in vitro.Our findings suggested that alamandine could mitigate retinal neovascularization by targeting the MrgD-mediated HIF-1α/VEGF pathway,providing a potential therapeutic agent for OIR prevention and treatment.
基金supported by the National Natural Science Foundation of China (Nos.42175084,21577126,and 41561144004)the Department of Science and Technology of China (Nos.2018YFC0213506,2018YFC0213503,and 2016YFC0202702)+4 种基金the National Research Program for Key Issues in Air Pollution Control in China (No.DQGG0107)supported by the“Zhejiang 1000 Talent Plan”and Research Center for Air Pollution and Health in Zhejiang UniversityPengfei Li is supported by the National Natural Science Foundation of China (No.22006030)the Initiation Fund for Introducing Talents of Hebei Agricultural University (No.412201904)the Hebei Youth Top Q15 Fund (No.BJ2020032)。
文摘To guarantee the blue skies for the 2022 Winter Olympics held in Beijing and Zhangjiakou from February 4 to 20,Beijing and its surrounding areas adopted a series of emission control measures.This provides an opportunity to determine the impacts of large-scale temporary control measures on the air quality in Beijing during this special period.Here,we applied the WRF-CMAQ model to quantify the contributions of emission reduction measures and meteorological conditions.Results show that meteorological conditions in 2022 decreased PM_(2.5)in Beijing by 6.9 and 11.8μg/m^(3)relative to 2021 under the scenarios with and without emission reductions,respectively.Strict emission reduction measures implemented in Beijing and seven neighboring provinces resulted in an average decrease of 13.0μg/m^(3)(-41.2%)in PM_(2.5)in Beijing.Over the entire period,local emission reductions contributed more to good air quality in Beijing than nonlocal emission reductions.Under the emission reduction scenario,local,controlled regions,other regions,and boundary conditions contributed 47.7%,42.0%,5.3%,and 5.0%to the PM_(2.5)concentrations in Beijing,respectively.The results indicate that during the cleaning period with the air masses from the northwest,the abatements of PM_(2.5)were mainly caused by local emission reductions.However,during the potential pollution period with the air masses from the east-northeast and west-southwest,the abatements of PM_(2.5)were caused by both local and nonlocal emission reductions almost equally.This implies that regional coordinated prevention and control strategies need to be arranged scientifically and rationally when heavy pollution events are forecasted.
基金supported by the National Natural Science Foundation of China(Grant No.31772024)。
文摘To investigate the roles of Harvista(a sprayable 1-methylcyclopropene,1-MCP,available component is 150 g·hm^(-2))and Ethephon(1 mL·L^(-1))applied at preharvest in apple(Malus domestica Borkh.'Golden Delicious')fruit cuticular wax biosynthesis,the expression of genes related to fruit cuticular wax biosynthesis and ethylene biosynthesis and signaling,ethylene production rate,respiration rate,wax constituents and structure,and fruit quality were determined at harvest and during cold storage.The results showed that 1-MCP inhibited the expression levels of ethylene biosynthesis and signaling-related genes,decreased fruit ethylene production,and inhibited the expression of Md LACS1,Md CER6,Md CER4 and Md WSD1,which resulted in decreases in alcohols,acids,and esters content in fruit cuticular wax.1-MCP also reduced fruit dropping rate from 17.17%to 12%;maintained fruit firmness,soluble solids,titratable acidity during cold storage;showed about as one fifth in the total length and one third in the widest width of wax crack as that in control at harvest.In contrast,Ethephon produced the opposite effects.In conclusion,1-MCP inhibited fruit ethylene biosynthesis and signaling,and influenced fruit cuticular wax biosynthesis.Thus,the fruit cuticular wax constituents and structure was altered,and the fruit quality were maintained at harvest and during cold storage.The results provide a new technology for improving apple fruit harvest and postharvest quality by preharvest application of sprayable 1-MCP.
文摘The new ＂4-to-1＂ school-enterprise cooperation model is constructed in combination with the automation program provided by Tianshi College. The new school-enterprise cooperation model is an effective way for Tianshi College to train high-quality skilled personnel, an important carrier to deepen the production-study-research cooperation education, and also an important part to improve the work quality of personnel cultivation. In China, it is an urgent need of the current employment situation, but also an important strategy for Tianshi College to attain a lon^-term stable develot^ment. In the orocess of imolementation, a win-win situation has been achieved bv constantly deenening reform.
基金supports from the National Natural Science Foundation of China(No.22109171)the Chongqing Talent Program(No.cstc2024ycjh-bgzxm0068))+1 种基金the Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJZD-K202301101)the Natural Science Foundation of Chongqing(No.CSTB2023NSCQ-MSX0315).
文摘Hydrogen energy carrier produced by water electrolysis in alkaline electrolytes is rather meaningful and significant for global sustainability imperatives,while the highpH condition usually leads to a poor reversibility of proton adsorption and desorption that significantly determines the hydrogen-generation activity in hydrogen evolution reaction(HER)process.Herein,we demonstrate a remarkable Ru-Mo solid-solution nanocrystal catalyst in alkaline HER process by a very simple but feasible pyrolysis and alkali leaching strategy.Benefiting from the pinning effect and local chemical-and electronic-structure regulations of Mo solute atoms,an ultra-low overpotential(17.3 mV)and an exceptional stability(>100 h)at the typical current density of 10 mA·cm^(−2)are achieved on the ultrasmall Ru-Mo solid-solution nanocrystal catalyst in 1.0 M KOH electrolyte.Density function theory(DFT)calculations gain an insight into the synergistic effect of neighboring Ru and Mo sites in alkaline HER process,where Mo solute atoms are beneficial for the adsorption and activation of water molecules for proton generation and accumulation due to their rich outermost 4d vacant orbitals,while the energy-favorable Ru sites are responsible for the fast deprotonation kinetics of hydrogen intermediates.Our work may provide an interesting route for the development of efficient and stable solid-solution alloy nanocrystals towards alkaline water electrolysis and beyond.
基金The information described in this article is covered by two Syngenta patent publications:WO20211108336 and WO2021108337.
文摘Efficient genotype-independent transformation and genome editing are highly desirable for plant biotechnology research and product development efforts.We have developed a novel approach to enable fast,high-throughput,and genotype-flexible Agrobacterium-mediated transformation using the important crop soybean as a test system.This new method is called GiFT(genotype-independent fast transformation)and involves only a few simple steps.The method uses germinated seeds as explants,and DNA delivery is achieved through Agrobacterium infection of wounded explants as in conventional in vitro-based methods.Following infection,the wounded explants are incubated in liquid medium with a sublethal level of selection and then transplanted directly into soil.The transplanted seedlings are then selected with herbicide spray for 3 weeks.The time required from initiation to fully established healthy T0 transgenic events is about 35 days.The GiFT method requires minimal in vitro manipulation or use of tissue culture media.Because the regeneration occurs in planta,the GiFT method is highly flexible with respect to genotype,which we demonstrate via successful transformation of elite germplasms from diverse genetic backgrounds.We also show that the soybean GiFT method can be applied to both conventional binary vectors and CRISPR-Cas12a vectors for genome editing applications.Analyses of T1 progeny demonstrate that the events have a high inheritance rate and can be used for genome engineering applications.By minimizing the need for tissue culture,the novel approach described here significantly improves operational efficiency while greatly reducing personnel and supply costs.It is the first industry-scale transformation method to utilize in planta selection in a major field crop.
基金National Natural Science Foundation of China(Grant No.81802151)Shandong Province Natural Science Foundation(Grant No.ZR2019MH012)+2 种基金China Post-doctoral Science Foundation(Grant No.2018M642616)Qingdao Applied Foundational Research Youth Project(Grant No.19-6-2-55-cg)Qingdao Traditional Chinese Medicine Science and Technology Project(Grant No.2021-zyym28)
文摘Background:Perturbations in bone marrow mesenchymal stem cell(BMSC)differentiation play an important role in steroid-induced osteonecrosis of the femoral head(SONFH).At present,studies on SONFH concentrate upon the balance within BMSC osteogenic and adipogenic differentiation.However,BMSC apoptosis as well as proliferation are important prerequisites in their differentiation.The hedgehog(HH)signaling pathway regulates bone cell apoptosis.Baicalin(BA),a well-known compound in traditional Chinese medicine,can affect the proliferation and apoptosis of numerous cell types via HH signaling.However,the potential role and mechanisms of BA on BMSCs are unclear.Thus,we aimed to explore the role of BA in dexamethasone(Dex)-induced BMSC apoptosis in this study.Methods:Primary BMSCs were treated with 10-6 mol/L Dex alone or with 5.0μmol/L,10.0μmol/L,or 50.0μmol/L BA for 24 hours followed by co-treatment with 5.0μmol/L,10.0μmol/L,or 50.0μmol/L BA and 10-6 mol/L Dex.Cell viability was assayed through the Cell Counting Kit-8(CCK-8).Cell apoptosis was evaluated using Annexin V-fluorescein isothiocyanate/propidium iodide(PI)staining followed by flow cytometry.The imaging and counting,respectively,of Hochest 33342/PI-stained cells were used to assess the morphological characteristics and proportion of apoptotic cells.To quantify the apoptosis-related proteins(e.g.,apoptosis regulator BAX[Bax],B-cell lymphoma 2[Bcl-2],caspase-3,and cleaved caspase-3)and HH signaling pathway proteins,western blotting was used.A HH-signaling pathway inhibitor was used to demonstrate that BA exerts its anti-apoptotic effects via the HH signaling pathway.Results:The results of CCK-8,Hoechst 33342/PI-staining,and flow cytometry showed that BA did not significantly promote cell proliferation(CCK-8:0μmol/L,100%;2.5μmol/L,98.58%;5.0μmol/L,95.18%;10.0μmol/L,98.11%;50.0μmol/L,99.38%,F=2.33,P>0.05),but it did attenuate the effect of Dex on apoptosis(Hoechst 33342/PI-staining:Dex+50.0μmol/L BA,12.27%vs.Dex,39.27%,t=20.62;flow cytometry:Dex+50.0μmol/L BA,12.68%vs.Dex,37.43%,t=11.56;Both P<0.05).The results of western blotting analysis showed that BA reversed Dex-induced apoptosis by activating the HH signaling pathway,which down-regulated the expression of Bax,cleaved-caspase 3,and suppressor of fused(SUFU)while up-regulating Bcl-2,sonic hedgehog(SHH),and zinc finger protein GLI-1(GLI-1)expression(Bax/Bcl-2:Dex+50.0μmol/L BA,1.09 vs.Dex,2.76,t=35.12;cleaved caspase-3/caspase-3:Dex+50.0μmol/L BA,0.38 vs.Dex,0.73,t=10.62;SHH:Dex+50.0μmol/L BA,0.50 vs.Dex,0.12,t=34.01;SUFU:Dex+50.0μmol/L BA,0.75 vs.Dex,1.19,t=10.78;GLI-1:Dex+50.0μmol/L BA,0.40 vs.Dex,0.11,t=30.68.All P<0.05).Conclusions:BA antagonizes Dex-induced apoptosis of human BMSCs by activating the HH signaling pathway.It is a potential candidate for preventing SONFH.
