Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explo...Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.展开更多
The integration, analysis and visualization of the big omics data are critical for addressing a broad spectrum of biological questions. One of the most frequently conducted procedures is enrichment analysis, which sta...The integration, analysis and visualization of the big omics data are critical for addressing a broad spectrum of biological questions. One of the most frequently conducted procedures is enrichment analysis, which statistically tests whether individual functional an- notations of Gent Ontology （GO） or Kyoto Encyclopedia of Genes and Genomes （KEGG） are significantly over-or under-represented in an ＂interesting＂ gene or protein list against the reference set （Tavazoie et al., 1999）.展开更多
In the context of the new period,the living standards and comfort demands of rural residents are increasing,which promotes the continuous growth of the total energy consumption of rural residential buildings.In this s...In the context of the new period,the living standards and comfort demands of rural residents are increasing,which promotes the continuous growth of the total energy consumption of rural residential buildings.In this study,it estimated the total energy consumption of rural residential buildings in 30 provinces(or cities)in China from 2004 to 2016.Through the division of climate regions of the residential buildings,this paper analyzed the characteristics of changes in the energy structure of residential buildings and the trend of energy consumption from the perspective of the province.Then based on the people s livelihood and from the perspective of development,it came up with some pertinent strategies and recommendations for energy saving and emission reduction in rural residential buildings.展开更多
This paper is devoted to the problem of stabilizing a Hopfield-type neural network with bi-directional ring architecture and two delays. The delay-independent and delay-dependent stability conditions are explicitly pr...This paper is devoted to the problem of stabilizing a Hopfield-type neural network with bi-directional ring architecture and two delays. The delay-independent and delay-dependent stability conditions are explicitly presented by the method of the characteristic roots and the skill of mathematical analysis. Moreover, if a link between the adjacent two neurons is cut, the ring neural network turns to a linear one, and the stability results are also established. Furthermore, a comparative analysis for the ring and linear network shows that the stability domain is enlarged after the breaking.展开更多
In microbial ecology,there is limited understanding of the mechanisms governing patterns in community structure across space and time.Here,we studied the changes of bacterial co-occurrence network structure over four ...In microbial ecology,there is limited understanding of the mechanisms governing patterns in community structure across space and time.Here,we studied the changes of bacterial co-occurrence network structure over four primary successional soils after glacier retreat,including a sand dune system and three glacier foreland series,varying in timescale from centuries to millennia.We found that in all series,network structure was most complex in the earliest stages of succession,and became simpler over time.Richness and abundance of keystone species and network stability also declined over time.It appears that with less productive,nutrient poor and physiologically extreme conditions of early succession,closer interactions among bacterial species are ecologically selected for.These may take the form of consortia(with positive interactions)or stronger niche exclusion(with negative interactions).Additionally,we quantified the relative roles of different structuring processes on bacterial community using a bin-based null model analysis(iCAMP).With each successional series,community composition was initially governed by stochasticity,but as succession proceeded there was a progressive increase in deterministic selection over time,correlated with decreasing pH.Overall,our results show a consistency among the four series in long-term processes of community succession,with more integrated networks and greater stochasticity in early stages.展开更多
Land application of anaerobic digestion(AD)effluent as a fertilizer is desirable for nutrient recycling,but often supplies excess phosphorus(P),which contributes to surface water eutrophication.Reducing the P content ...Land application of anaerobic digestion(AD)effluent as a fertilizer is desirable for nutrient recycling,but often supplies excess phosphorus(P),which contributes to surface water eutrophication.Reducing the P content in AD effluent filtrate using calcium(Ca)treatment prior to land application is a potential strategy for improving effluent disposal and meeting the discharge standard.This study took flue gas desulphurization(FGD)gypsum,a by-product of coal-fired power plants,as a low-cost Ca source,and combined with traditional phosphorus removal agents to achieve high phosphorus removal efficiency with less chemical cost.As the results showed,FGD gypsum dosages of 20 mmol/L Ca(3.44 g/L)and 40 mmol/L Ca(6.89 g/L)removed up to 97.1%of soluble P(initially 102.8 mg/L)within 60-90 minutes.Combining FGD gypsum treatment with traditional chemical treatments using calcium hydroxide[Ca(OH)2]or ferric chloride(FeCl3)could achieve>99%P removal with reduced chemical costs.This study demonstrated that FGD gypsum is an efficient calcium-based precipitant for phosphorus removal,offering a cost-effective and sustainable approach to enhance wastewater treatment practices and meet discharge standards in wastewater management.展开更多
Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of ESCC patients. Here, we found benzydamine, a locally acti...Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of ESCC patients. Here, we found benzydamine, a locally acting non-steroidal anti-inflammatory drug, had potent cytotoxic effect on ESCC cells. Benzydamine could suppress ESCC proliferation in vivo and in vitro. In terms of mechanism, CDK2 was identified as a target of benzydamine by molecular docking, pull-down assay and in vitro kinase assay. Specifically, benzydamine inhibited the growth of ESCC cells by inhibiting CDK2 activity and affecting downstream phosphorylation of MCM2, c-Myc and Rb, resulting in cell cycle arrest. Our study illustrates that benzydamine inhibits the growth of ESCC cells by downregulating the CDK2 pathway.展开更多
As an important protein acylation modification,lysine succinylation(Ksucc)is involved in diverse biological processes,and participates in human tumorigenesis.Here,we collected 26,243 non-redundant known Ksucc sites fr...As an important protein acylation modification,lysine succinylation(Ksucc)is involved in diverse biological processes,and participates in human tumorigenesis.Here,we collected 26,243 non-redundant known Ksucc sites from 13 species as the benchmark data set,combined 10 types of informative features,and implemented a hybrid-learning architecture by integrating deep-learning and conventional machine-learning algorithms into a single framework.We constructed a new tool named HybridSucc,which achieved area under curve(AUC)values of 0.885 and 0.952 for general and human-specific prediction of Ksucc sites,respectively.In comparison,the accuracy of HybridSucc was 17.84%-50.62%better than that of other existing tools.Using HybridSucc,we conducted a proteome-wide prediction and prioritized 370 cancer mutations that change Ksucc states of 218 important proteins,including PKM2,SHMT2,and IDH2.We not only developed a high-profile tool for predicting Ksucc sites,but also generated useful candidates for further experimental consideration.The online service of HybridSucc can be freely accessed for academic research at http://hybridsucc.biocuckoo.org/.展开更多
基金supported by the National Natural Science Foundation of China(32202634)National Natural Science Foundation of China(31961143020)+2 种基金National Natural Science Foundation of China(U20A2052)China Agricultural Research System(CARS-35)the Heilongjiang Provincial Research Institutes Research Business Fund Project(CZKYF2023-1-B009)。
文摘Cold stress in low-temperature environments can trigger changes in gene expression,but epigenomics regulation of temperature stability in vital tissues,including the fat and diencephalon,is still unclear.Here,we explore the cold-induced changes in epigenomic features in the diencephalon and fat tissues of two cold-resistant Chinese pig breeds,Min and Enshi black(ES)pigs,utilizing H3K27ac CUT&Tag,RNA-seq,and selective signature analysis.Our results show significant alterations in H3K27ac modifications in the diencephalon of Min pigs and the fat of ES pigs after cold exposure.Dramatic changes in H3K27ac modifications in the diencephalon of Min pig are primarily associated with genes involved in energy metabolism and hormone regulation,whereas those in the fat of ES pig are primarily associated with immunity-related genes.Moreover,transcription factors PRDM1 and HSF1,which show evidence of selection,are enriched in genomic regions presenting cold-responsive alterations in H3K27ac modification in the Min pig diencephalon and ES pig fat,respectively.Our results indicate the diversity of epigenomic response mechanisms to cold exposure between Min and ES pigs,providing unique epigenetic resources for studies of low-temperature adaptation in large mammals.
基金supported by the Special Project on Precision Medicine under the National Key R&D Program (2017YFC0906600)the Natural Science Foundation of China (No. 31671360)
文摘The integration, analysis and visualization of the big omics data are critical for addressing a broad spectrum of biological questions. One of the most frequently conducted procedures is enrichment analysis, which statistically tests whether individual functional an- notations of Gent Ontology （GO） or Kyoto Encyclopedia of Genes and Genomes （KEGG） are significantly over-or under-represented in an ＂interesting＂ gene or protein list against the reference set （Tavazoie et al., 1999）.
基金Student s Platform for Innovation and Entrepreneurship Training Program at State Level,the Ministry of Education of China(201910414024)Student s Platform for Innovation and Entrepreneurship Training Program at State Level,the Ministry of Education of China(201910414012).
文摘In the context of the new period,the living standards and comfort demands of rural residents are increasing,which promotes the continuous growth of the total energy consumption of rural residential buildings.In this study,it estimated the total energy consumption of rural residential buildings in 30 provinces(or cities)in China from 2004 to 2016.Through the division of climate regions of the residential buildings,this paper analyzed the characteristics of changes in the energy structure of residential buildings and the trend of energy consumption from the perspective of the province.Then based on the people s livelihood and from the perspective of development,it came up with some pertinent strategies and recommendations for energy saving and emission reduction in rural residential buildings.
文摘This paper is devoted to the problem of stabilizing a Hopfield-type neural network with bi-directional ring architecture and two delays. The delay-independent and delay-dependent stability conditions are explicitly presented by the method of the characteristic roots and the skill of mathematical analysis. Moreover, if a link between the adjacent two neurons is cut, the ring neural network turns to a linear one, and the stability results are also established. Furthermore, a comparative analysis for the ring and linear network shows that the stability domain is enlarged after the breaking.
文摘In microbial ecology,there is limited understanding of the mechanisms governing patterns in community structure across space and time.Here,we studied the changes of bacterial co-occurrence network structure over four primary successional soils after glacier retreat,including a sand dune system and three glacier foreland series,varying in timescale from centuries to millennia.We found that in all series,network structure was most complex in the earliest stages of succession,and became simpler over time.Richness and abundance of keystone species and network stability also declined over time.It appears that with less productive,nutrient poor and physiologically extreme conditions of early succession,closer interactions among bacterial species are ecologically selected for.These may take the form of consortia(with positive interactions)or stronger niche exclusion(with negative interactions).Additionally,we quantified the relative roles of different structuring processes on bacterial community using a bin-based null model analysis(iCAMP).With each successional series,community composition was initially governed by stochasticity,but as succession proceeded there was a progressive increase in deterministic selection over time,correlated with decreasing pH.Overall,our results show a consistency among the four series in long-term processes of community succession,with more integrated networks and greater stochasticity in early stages.
基金supported by the Shaanxi Province Science Foundation for Youths(Grant No.2023-JC-QN-0202)the Technology Innovation Center for Land Engineering and Human Settlements(Grant No.201912131-D2)+1 种基金the Shaanxi Province Key Research and Development Projects(Grant No.2022ZDLNY02-07)the“Young Talent Starting Fund”,and“Human Environment Improvements and Resources Utilization in Rural Areas”Research Projects of Xi’an Jiaotong University(Grant No.202012435).
文摘Land application of anaerobic digestion(AD)effluent as a fertilizer is desirable for nutrient recycling,but often supplies excess phosphorus(P),which contributes to surface water eutrophication.Reducing the P content in AD effluent filtrate using calcium(Ca)treatment prior to land application is a potential strategy for improving effluent disposal and meeting the discharge standard.This study took flue gas desulphurization(FGD)gypsum,a by-product of coal-fired power plants,as a low-cost Ca source,and combined with traditional phosphorus removal agents to achieve high phosphorus removal efficiency with less chemical cost.As the results showed,FGD gypsum dosages of 20 mmol/L Ca(3.44 g/L)and 40 mmol/L Ca(6.89 g/L)removed up to 97.1%of soluble P(initially 102.8 mg/L)within 60-90 minutes.Combining FGD gypsum treatment with traditional chemical treatments using calcium hydroxide[Ca(OH)2]or ferric chloride(FeCl3)could achieve>99%P removal with reduced chemical costs.This study demonstrated that FGD gypsum is an efficient calcium-based precipitant for phosphorus removal,offering a cost-effective and sustainable approach to enhance wastewater treatment practices and meet discharge standards in wastewater management.
基金supported by the National Natural Science Foundations of China(No.81872335)the National Natural Science Youth Foundation(No.81902486)+2 种基金the Natural Science Foundation of Henan(No.161100510300)the Central Plains Science and Technology Innovation Leading Talents(No.224200510015)the Science and Technology Project of Henan Province(No.212102310187).
文摘Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of ESCC patients. Here, we found benzydamine, a locally acting non-steroidal anti-inflammatory drug, had potent cytotoxic effect on ESCC cells. Benzydamine could suppress ESCC proliferation in vivo and in vitro. In terms of mechanism, CDK2 was identified as a target of benzydamine by molecular docking, pull-down assay and in vitro kinase assay. Specifically, benzydamine inhibited the growth of ESCC cells by inhibiting CDK2 activity and affecting downstream phosphorylation of MCM2, c-Myc and Rb, resulting in cell cycle arrest. Our study illustrates that benzydamine inhibits the growth of ESCC cells by downregulating the CDK2 pathway.
基金supported by the Special Project on Precision Medicine under the National Key R&D Program of China(Grant Nos.2017YFC0906600 and 2018YFC0910500)the National Natural Science Foundation of China(Grant Nos.31671360,31801095,and 31601067)+4 种基金Fundamental Research Funds for the Central Universities(Grant Nos.2019kfyRCPY043 and 2017KFXKJC001)the National Program for Support of Top-Notch Young ProfessionalsChangjiang Scholars Program of Chinaprogram for HUST Academic Frontier Youth TeamChina Postdoctoral Science Foundation(Grant No.2018M632870)
文摘As an important protein acylation modification,lysine succinylation(Ksucc)is involved in diverse biological processes,and participates in human tumorigenesis.Here,we collected 26,243 non-redundant known Ksucc sites from 13 species as the benchmark data set,combined 10 types of informative features,and implemented a hybrid-learning architecture by integrating deep-learning and conventional machine-learning algorithms into a single framework.We constructed a new tool named HybridSucc,which achieved area under curve(AUC)values of 0.885 and 0.952 for general and human-specific prediction of Ksucc sites,respectively.In comparison,the accuracy of HybridSucc was 17.84%-50.62%better than that of other existing tools.Using HybridSucc,we conducted a proteome-wide prediction and prioritized 370 cancer mutations that change Ksucc states of 218 important proteins,including PKM2,SHMT2,and IDH2.We not only developed a high-profile tool for predicting Ksucc sites,but also generated useful candidates for further experimental consideration.The online service of HybridSucc can be freely accessed for academic research at http://hybridsucc.biocuckoo.org/.
