Phosphorus use efficiency(PUE)can be improved through cultivation techniques and breeding.However,little is known about rice(Oryza sativa L.)agronomic and physiological traits associated with high PUE.We characterized...Phosphorus use efficiency(PUE)can be improved through cultivation techniques and breeding.However,little is known about rice(Oryza sativa L.)agronomic and physiological traits associated with high PUE.We characterized the agronomic and physiological traits of rice varieties with different tolerances to low phosphorus in nutrient solution.Two varieties with strong tolerance to low phosphorus(STVs)and two with weak tolerance(WTVs)were grown at normal(NP,control)and low phosphorus(LP,1/20 of NP)concentrations.Plants grown at LP produced significantly lower grain yield than those grown at NP.WTVs yields were lower than STVs yields.Compared to NP,LP significantly increased phosphorus translocation efficiency(PTE),internal phosphorus efficiency(IPE)and phosphorus harvest index(PHI).Under the LP condition,PTE and IPE were higher for STVs than for WTVs.LP also reduced tiller number,shoot biomass,leaf area index(LAI),leaf photosynthetic rate,and mean root diameter of both kinds of varieties at the main growth stages,but to a lower extent in STVs.LP significantly increased the number of productive tillers,root biomass,root-shoot ratio,root bleeding rate,and root acid phosphatase(RAP)activity.Total root length,root oxidation activity(ROA),and root total and active absorbing surface areas for STVs were significantly increased under LP,whereas the opposite responses were observed for WTVs.Total root length,ROA,root bleeding rate,root active absorbing surface area,and RAP activity were positively and significantly correlated with grain yield,PTE,and IPE.These results suggest that the tolerance of rice varieties to a low-phosphorus growth condition is closely associated with root growth with higher biomass and activity.展开更多
Objective： To investigate the proteornic differences between the high-sensitivity （HS） group and low-sensitivity （LS） group of cervical cancer treated by radiotherapy and confirm the radiotherapy sensitivity asso...Objective： To investigate the proteornic differences between the high-sensitivity （HS） group and low-sensitivity （LS） group of cervical cancer treated by radiotherapy and confirm the radiotherapy sensitivity associated proteins in early cervical cancer. Methods： The fresh carcinoma tissues were collected from 10 untreated cervical cancer patients and preserved in the -80 ℃ refrigeratory. The tissues were classified into two groups： high sensitivity group （HS） and low sensitivity group （LS）, according to their response to radiotherapy. In the first part of our experiment, protein separating was performed by using two-dimensional gel electrophoresis （2-DE） with Amersham 18 cm linear pH 3-10 immobilized pH gradient （IPG） strips. The images of the gels were acquired by the scanner and then analyzed by using PD-quest7.3 software to find the differentially expression protein-spots in each group. Then the differentially expressed protein-spots was incised from the gels and digested by trypsin. The peptide mass fingerprintings （PMF） was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and the proteins were identified by data searching in the Mascot-database. Part of differentially expression proteins were assayed by Western Blot. Results： Most of the gels were clear and successfully analyzed by PD-quest7.3 software. Most of the protein-spots concentrated on the area of 20-100Kda （Mw） and pH4-8. The average number of the protein-spots was 754 ± 64 in HS group and 777 ±48个 in LS group. The match rate was 87.6% between two groups. Five high expression proteins were found in HS group which were low expression in LS group, 3 high expression protein were found in LS group which were low expression in HS group. Reselts of Western Blot were in coincidence to proteomic result. Conclusion： The 2-DE gels image of HS group and LS group with early cervical cancer tissues treated by radiotherapy are successfully acquired. Some differentially expression proteins between the two groups are further confirmed by immunohistochemical assay.展开更多
Objective： Colorectal carcinoma clinical stage associated proteins would be found by comparing differential expressed proteins from colorectal carcinoma tissues with different clinical stages. Methods： Total protein...Objective： Colorectal carcinoma clinical stage associated proteins would be found by comparing differential expressed proteins from colorectal carcinoma tissues with different clinical stages. Methods： Total protein from colorectal carcinoma tissues were extracted; differential proteome profiles were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis （2-DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results： Well-resolved, reproducible 2-DE profiles of human colorectal carcinoma tissues were obtained. Average protein spots were 970 ± 41,980 ± 32, 1010 ± 43, 1240 ±34 in stage Ⅰ, stage Ⅱ, stage Ⅲ, stage Ⅳ respectively; Compared to stage Ⅰ, differential expressed protein spots was 52.00 ± 12 in stage Ⅱ, 42.00 ± 11 in stage Ⅲ, 72.00 ± 15 in stage Ⅳ; Part of differential expressing proteins were analyzed by mass spectrometry and bioinformation, 19 of them were well characterized. Three proteins were overexpressed in stage Ⅰ, stage Ⅲ, stage Ⅳ, and one protein were overexpressed in stage Ⅳ exclusively. Conclusion： Differential expressed proteins exist in clinical stage of colorectal carcinoma, which would be biomarkers for diagnosis and prediction of prognosis.展开更多
ADC189 is a novel drug of cap-dependent endonuclease inhibitor.In our study,its antiviral efficacy was evaluated in vitro and in vivo,and compared with baloxavir marboxil and oseltamivir.A first-in-human phase I study...ADC189 is a novel drug of cap-dependent endonuclease inhibitor.In our study,its antiviral efficacy was evaluated in vitro and in vivo,and compared with baloxavir marboxil and oseltamivir.A first-in-human phase I study in healthy volunteers included single ascending dose(SAD)and food effect(FE)parts.In the preclinical study,ADC189 showed potent antiviral activity against various types of influenza viruses,including H1N1,H3N2,influenza B virus,and highly pathogenic avian influenza,comparable to baloxavir marboxil.Additionally,ADC189 exhibited much better antiviral efficacy than oseltamivir in H1N1 infected mice.In the phase I study,ADC189 was rapidly metabolized to ADC189-I07,and its exposure increased proportionally with the dose.The terminal elimination half-life(T1/2)ranged from 76.69 to 98.28 hours.Of note,food had no effect on the concentration,clearance,and exposure of ADC189.It was well tolerated,with few treatment-emergent adverse events(TEAEs)reported and no serious adverse events(SAEs).ADC189 demonstrated excellent antiviral efficacy both in vitro and in vivo.It was safe,well-tolerated,and had favorable pharmacokinetic characteristics in healthy volunteers,supporting its potential for single oral dosing in clinical practice.展开更多
Accurate sag source location and precise sag type recognition are both essential to verifying the responsible party for the sag and taking countermeasures to improve power quality.In this paper,an attention-based inde...Accurate sag source location and precise sag type recognition are both essential to verifying the responsible party for the sag and taking countermeasures to improve power quality.In this paper,an attention-based independently recurrent neural network(IndRNN)for sag source location and sag type recognition in sparsely monitored power system is proposed.Specially,the given inputs are voltage waveforms collected by limited meters in sparsely monitored power system,and the desired outputs simultaneously contain the following information:the located lines where sag occurs;the corresponding sag types,including motor starting,transformer energizing and short circuit;and the fault phase for short circuit.In essence,the responsibility of the proposed method is to automatically establish a nonlinear function that relates the given inputs to the desired outputs with categorization labels as few as possible.A favorable feature of the proposed method is that it can be realized without system parameters or models.The proposed method is validated by IEEE 30-bus system and a real 134-bus system.Experimental results demonstrate that the accuracy of sag source location is higher than 99%for all lines,and the accuracy of sag type recognition is also higher than 99%for various sag sources including motor starting,transformer energizing and 7 different types of short circuits.Furthermore,a comparison among different monitor placements for the proposed method is conducted,which illustrates that the observability of power networks should be ensured to achieve satisfactory performance.展开更多
Commercial lithium(Li)-ion batteries(LIBs)are approaching their theoretical limits in energy density.As a result,Li metal batteries(LMBs)with either liquid or solid-state electrolytes have been proposed as a next-gene...Commercial lithium(Li)-ion batteries(LIBs)are approaching their theoretical limits in energy density.As a result,Li metal batteries(LMBs)with either liquid or solid-state electrolytes have been proposed as a next-generation alternative,although they currently pose major safety and stability issues.To resolve these issues,most research has focused on the development and production of novelmaterials and coatings.Although promising performance benchmarks have been achieved,these strategiesmay generate issues for large-scale production,due to the added costs associated with using novel materials and developing the required cell fabrication process and infrastructure.Optimizing external conditions,such as selecting specific cell cycling protocols,testing the cell or synthesizing materials at specific temperatures,testing or fabricating the cell under specific mechanical pressures,etc.,are often overlooked as a means of directly improving battery performance without requiring complex material modifications.In this review,we will discuss how these external parameters can address the key failure mechanisms and challenges in LMBs that use either liquid or solid electrolytes.Similarities and differences in mechanisms(Li+transport,Li dendrite propagation,thermal effects,etc.)observed in liquid and solid-state configurations will also be discussed.Finally,we propose the outstanding scientific and economic gaps in LMBs,thereby providing future directions to explore.展开更多
基金supported by the National Key Research and Development Program(2016YFD0300206-4,2018YFD0300800)the National Natural Science Foundation of China(31461143015,31771710,31871559)+4 种基金Young Elite Scientists Sponsorship Program by CAST(2016QNRC001)Six Talent Peaks Project in Jiangsu Province(SWYY-151)the Jiangsu Provincial Key Research and Development Program(Modern Agriculture)(BE2015320)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Top Talent Supporting Program of Yangzhou University(2015-01)。
文摘Phosphorus use efficiency(PUE)can be improved through cultivation techniques and breeding.However,little is known about rice(Oryza sativa L.)agronomic and physiological traits associated with high PUE.We characterized the agronomic and physiological traits of rice varieties with different tolerances to low phosphorus in nutrient solution.Two varieties with strong tolerance to low phosphorus(STVs)and two with weak tolerance(WTVs)were grown at normal(NP,control)and low phosphorus(LP,1/20 of NP)concentrations.Plants grown at LP produced significantly lower grain yield than those grown at NP.WTVs yields were lower than STVs yields.Compared to NP,LP significantly increased phosphorus translocation efficiency(PTE),internal phosphorus efficiency(IPE)and phosphorus harvest index(PHI).Under the LP condition,PTE and IPE were higher for STVs than for WTVs.LP also reduced tiller number,shoot biomass,leaf area index(LAI),leaf photosynthetic rate,and mean root diameter of both kinds of varieties at the main growth stages,but to a lower extent in STVs.LP significantly increased the number of productive tillers,root biomass,root-shoot ratio,root bleeding rate,and root acid phosphatase(RAP)activity.Total root length,root oxidation activity(ROA),and root total and active absorbing surface areas for STVs were significantly increased under LP,whereas the opposite responses were observed for WTVs.Total root length,ROA,root bleeding rate,root active absorbing surface area,and RAP activity were positively and significantly correlated with grain yield,PTE,and IPE.These results suggest that the tolerance of rice varieties to a low-phosphorus growth condition is closely associated with root growth with higher biomass and activity.
基金Supported by grants from the Hunan Natural Science foundation (No.06JJ4199)the Hunan Science Technology Foundation (No.2007SK3010)
文摘Objective： To investigate the proteornic differences between the high-sensitivity （HS） group and low-sensitivity （LS） group of cervical cancer treated by radiotherapy and confirm the radiotherapy sensitivity associated proteins in early cervical cancer. Methods： The fresh carcinoma tissues were collected from 10 untreated cervical cancer patients and preserved in the -80 ℃ refrigeratory. The tissues were classified into two groups： high sensitivity group （HS） and low sensitivity group （LS）, according to their response to radiotherapy. In the first part of our experiment, protein separating was performed by using two-dimensional gel electrophoresis （2-DE） with Amersham 18 cm linear pH 3-10 immobilized pH gradient （IPG） strips. The images of the gels were acquired by the scanner and then analyzed by using PD-quest7.3 software to find the differentially expression protein-spots in each group. Then the differentially expressed protein-spots was incised from the gels and digested by trypsin. The peptide mass fingerprintings （PMF） was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and the proteins were identified by data searching in the Mascot-database. Part of differentially expression proteins were assayed by Western Blot. Results： Most of the gels were clear and successfully analyzed by PD-quest7.3 software. Most of the protein-spots concentrated on the area of 20-100Kda （Mw） and pH4-8. The average number of the protein-spots was 754 ± 64 in HS group and 777 ±48个 in LS group. The match rate was 87.6% between two groups. Five high expression proteins were found in HS group which were low expression in LS group, 3 high expression protein were found in LS group which were low expression in HS group. Reselts of Western Blot were in coincidence to proteomic result. Conclusion： The 2-DE gels image of HS group and LS group with early cervical cancer tissues treated by radiotherapy are successfully acquired. Some differentially expression proteins between the two groups are further confirmed by immunohistochemical assay.
文摘Objective： Colorectal carcinoma clinical stage associated proteins would be found by comparing differential expressed proteins from colorectal carcinoma tissues with different clinical stages. Methods： Total protein from colorectal carcinoma tissues were extracted; differential proteome profiles were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis （2-DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results： Well-resolved, reproducible 2-DE profiles of human colorectal carcinoma tissues were obtained. Average protein spots were 970 ± 41,980 ± 32, 1010 ± 43, 1240 ±34 in stage Ⅰ, stage Ⅱ, stage Ⅲ, stage Ⅳ respectively; Compared to stage Ⅰ, differential expressed protein spots was 52.00 ± 12 in stage Ⅱ, 42.00 ± 11 in stage Ⅲ, 72.00 ± 15 in stage Ⅳ; Part of differential expressing proteins were analyzed by mass spectrometry and bioinformation, 19 of them were well characterized. Three proteins were overexpressed in stage Ⅰ, stage Ⅲ, stage Ⅳ, and one protein were overexpressed in stage Ⅳ exclusively. Conclusion： Differential expressed proteins exist in clinical stage of colorectal carcinoma, which would be biomarkers for diagnosis and prediction of prognosis.
基金sponsored by Jiaxing Andicon Biotech Co.,Ltd.Jiaxing Andicon Biotech Co.,Ltd.is responsible for the initiation,management,and funding of this study.
文摘ADC189 is a novel drug of cap-dependent endonuclease inhibitor.In our study,its antiviral efficacy was evaluated in vitro and in vivo,and compared with baloxavir marboxil and oseltamivir.A first-in-human phase I study in healthy volunteers included single ascending dose(SAD)and food effect(FE)parts.In the preclinical study,ADC189 showed potent antiviral activity against various types of influenza viruses,including H1N1,H3N2,influenza B virus,and highly pathogenic avian influenza,comparable to baloxavir marboxil.Additionally,ADC189 exhibited much better antiviral efficacy than oseltamivir in H1N1 infected mice.In the phase I study,ADC189 was rapidly metabolized to ADC189-I07,and its exposure increased proportionally with the dose.The terminal elimination half-life(T1/2)ranged from 76.69 to 98.28 hours.Of note,food had no effect on the concentration,clearance,and exposure of ADC189.It was well tolerated,with few treatment-emergent adverse events(TEAEs)reported and no serious adverse events(SAEs).ADC189 demonstrated excellent antiviral efficacy both in vitro and in vivo.It was safe,well-tolerated,and had favorable pharmacokinetic characteristics in healthy volunteers,supporting its potential for single oral dosing in clinical practice.
基金This work was partly supported by National Natural Science Foundation of China(No.61903296)Key Project of Natural Science Basic Research Plan in Shaanxi Province of China(No.2019ZDLGY18-03)+1 种基金Thousand Talents Plan of Shaanxi Province for Young Professionals,Project of Shaanxi Science and Technology(No.2019JQ-329)Doctoral Scientific Research Foundation of Xi’an University of Technology(No.103-451116012).
文摘Accurate sag source location and precise sag type recognition are both essential to verifying the responsible party for the sag and taking countermeasures to improve power quality.In this paper,an attention-based independently recurrent neural network(IndRNN)for sag source location and sag type recognition in sparsely monitored power system is proposed.Specially,the given inputs are voltage waveforms collected by limited meters in sparsely monitored power system,and the desired outputs simultaneously contain the following information:the located lines where sag occurs;the corresponding sag types,including motor starting,transformer energizing and short circuit;and the fault phase for short circuit.In essence,the responsibility of the proposed method is to automatically establish a nonlinear function that relates the given inputs to the desired outputs with categorization labels as few as possible.A favorable feature of the proposed method is that it can be realized without system parameters or models.The proposed method is validated by IEEE 30-bus system and a real 134-bus system.Experimental results demonstrate that the accuracy of sag source location is higher than 99%for all lines,and the accuracy of sag type recognition is also higher than 99%for various sag sources including motor starting,transformer energizing and 7 different types of short circuits.Furthermore,a comparison among different monitor placements for the proposed method is conducted,which illustrates that the observability of power networks should be ensured to achieve satisfactory performance.
文摘Commercial lithium(Li)-ion batteries(LIBs)are approaching their theoretical limits in energy density.As a result,Li metal batteries(LMBs)with either liquid or solid-state electrolytes have been proposed as a next-generation alternative,although they currently pose major safety and stability issues.To resolve these issues,most research has focused on the development and production of novelmaterials and coatings.Although promising performance benchmarks have been achieved,these strategiesmay generate issues for large-scale production,due to the added costs associated with using novel materials and developing the required cell fabrication process and infrastructure.Optimizing external conditions,such as selecting specific cell cycling protocols,testing the cell or synthesizing materials at specific temperatures,testing or fabricating the cell under specific mechanical pressures,etc.,are often overlooked as a means of directly improving battery performance without requiring complex material modifications.In this review,we will discuss how these external parameters can address the key failure mechanisms and challenges in LMBs that use either liquid or solid electrolytes.Similarities and differences in mechanisms(Li+transport,Li dendrite propagation,thermal effects,etc.)observed in liquid and solid-state configurations will also be discussed.Finally,we propose the outstanding scientific and economic gaps in LMBs,thereby providing future directions to explore.
