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枯草芽孢杆菌fmb60菌株非核糖体肽类化合物对铜绿微囊藻生长的抑制作用 被引量:4
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作者 杨杰 王自山 +5 位作者 柴金龙 陈扬 卢静 房耀维 王淑军 陆兆新 《生物工程学报》 CAS CSCD 北大核心 2021年第2期625-634,共10页
铜绿微囊藻是一种分布广泛的水华微藻,可对人类健康和生态环境造成严重危害,而枯草芽孢杆菌作为一种生防微生物可通过非核糖体肽合成酶合成多种生物活性物质。因此,枯草芽孢杆菌fmb60非核糖体肽(Non-ribosomal peptide,NRP)类产物对铜... 铜绿微囊藻是一种分布广泛的水华微藻,可对人类健康和生态环境造成严重危害,而枯草芽孢杆菌作为一种生防微生物可通过非核糖体肽合成酶合成多种生物活性物质。因此,枯草芽孢杆菌fmb60非核糖体肽(Non-ribosomal peptide,NRP)类产物对铜绿微囊藻生长抑制作用的研究在水华治理方面具有重要的意义。利用基因组挖掘技术从枯草芽孢杆菌fmb60中分离鉴定出bacillibactin、表面活性素(Surfactin)和芬芥素(Fengycin)3种NRP类产物,进而通过添加不同浓度的NRP类产物研究其对铜绿微囊藻生长的影响,结果显示其对铜绿微囊藻4 d的半效应浓度值EC50.4 d为26.5 mg/L,且随着样品浓度的增加,枯草芽孢杆菌fmb60的NRP类产物对铜绿微囊藻的抑制作用增强。当向其分别加入50 mg/L的样品时,培养4 d的铜绿微囊藻Fv/Fm、Fv/Fo、Yield参数分别比对照组降低了2.8%、1.7%、2.0%。表明枯草芽孢杆菌fmb60 NRP类产物能够显著抑制铜绿微囊藻的光合作用强度及代谢合成,从而为枯草芽孢杆菌抑藻剂的开发奠定理论基础。 展开更多
关键词 枯草芽孢杆菌 非核糖体肽类产物 铜绿微囊藻 生长抑制
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Changes in antioxidant and flavor profiles of raspberry,blackberry,and mulberry juices fermented by urolithin A-producing Limosilactobacillus fermentum FUA033
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作者 Ziyan Hua Yingying Bian +6 位作者 Fengxia Lu Shu Liu Wenyue Ma Tao Chen Juan Feng Yanqiu Xia yaowei fang 《Food Bioscience》 2025年第3期1412-1421,共10页
Consumers are growing interest in foods that promote health and offer improved nutritional benefits.Berries,including raspberry,blackberry,and mulberry,are rich in bioactive compounds,such as polyphenols and flavonoid... Consumers are growing interest in foods that promote health and offer improved nutritional benefits.Berries,including raspberry,blackberry,and mulberry,are rich in bioactive compounds,such as polyphenols and flavonoids,which are recognized for their antioxidant and anti-inflammatory properties.Nonetheless,the bioavailability of these compounds,particularly ellagitannins(ETs)and ellagic acid(EA),is often restricted.To overcome this challenge,Limosilactobacillus fermentum FUA033 was employed to ferment raspberry,blackberry,and mulberry juices,with the objective of enhancing bioactivity and improving flavor.Headspace solid-phase microextraction gas chromatography-mass spectrometry(HS-SPME-GC-MS)was utilized to identify changes in volatile organic compounds(VOCs)that contribute to the flavor profiles of the fermented juices.The results indicated that fermentation with L.fermentum FUA033 significantly elevated the levels of polyphenols and flavonoids,thereby increasing antioxidant activity and facilitating the conversion of EA to urolithin A.Additionally,HS-SPME-GC-MS analysis demonstrated that fermentation improved the flavor characteristics of berry juices by increasing the content of VOCs,such as terpenes and esters.These findings provide a systematic illustration of the potential of L.fermentum FUA033 to enhance the functional properties of berry juices and offer novel insights for the development of innovative functional foods. 展开更多
关键词 Fermentation Limosilactobacillus fermentum FUA033 Flavor profiles Berry juices Urolithin a Metabolomics
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