Currently,stem cell transplantations in cardiac repair are limited owing to disadvantages,such as immunological rejection and poor cell viability.Although direct injection of exosomes can have a curative effect simila...Currently,stem cell transplantations in cardiac repair are limited owing to disadvantages,such as immunological rejection and poor cell viability.Although direct injection of exosomes can have a curative effect similar to that of stem cell transplantation,high clearance hinders its application in clinical practice.Previous reports suggested that induction of coronary collateralization can be a desired method of adjunctive therapy for someone who had missed the optimal operation time to attenuate myocardial ischemia.In this study,to mimic the paracrine and biological activity of stem cells,we developed artificial stem cells that can continuously release Tβ4-exosomes(Tβ4-ASCs)by encapsulating specific exosomes within microspheres using microfluidics technology.The results show that Tβ4-ASCs can greatly promote coronary collateralization in the periphery of the myocardial infarcted area,and its therapeutic effect is superior to that of directly injecting the exosomes.In addition,to better understand how it works,we demonstrated that the Tβ4-ASC-derived exosomes can enhance the angiogenic capacity of coronary endothelial cells(CAECs)via the miR-17-5p/PHD3/Hif-1αpathway.In brief,as artificial stem cells,Tβ4-ASCs can constantly release functional exosomes and stimulate the formation of collateral circulation after myocardial infarction,providing a feasible and alternative method for clinical revascularization.展开更多
Organ-on-a-chip stands as a pivotal platform for skeletal muscle research while constructing 3D skeletal muscle tissues that possess both macroscopic and microscopic structures remains a considerable challenge.This st...Organ-on-a-chip stands as a pivotal platform for skeletal muscle research while constructing 3D skeletal muscle tissues that possess both macroscopic and microscopic structures remains a considerable challenge.This study draws inspiration from LEGO-like assembly,employing a modular approach to construct muscle tissue that integrates biomimetic macroscopic and microscopic structures.Modular LEGO-like hybrid nanofibrous scaffold bricks were fabricated by the combination of 3D printing and electrospinning techniques.Skeletal muscle cells cultured on these modular scaffold bricks exhibited a highly orientated nanofibrous structure.A variety of construction of skeletal muscle tissues further enabled development by various assembling processes.Moreover,skeletal muscle-on-a-chip(SMoC)was further assembled as a functional platform for electrical or perfusion stimuli investigation.The electrical stimulus was conveniently applied and tuned in such a SMoC platform to significantly enhance the differentiation of skeletal muscle tissues.Additionally,the effect of perfusion stimulation on skeletal muscle vascularization within the SMoC platform was also demonstrated.These findings highlight the potential of these assembled SMoCs as functional ex vivo platforms for skeletal tissue engineering and drug research applications,and such a LEGO-like assembly strategy could also be applied to the other engineering organ-on-chips fabrication,which facilitates the development of bionic functional platforms for various biomedical research applications.展开更多
Heart-on-a-chip(HoC)has emerged as a highly efficient,cost-effective device for the development of engineered cardiac tissue,facilitating high-throughput testing in drug development and clinical treatment.HoC is prima...Heart-on-a-chip(HoC)has emerged as a highly efficient,cost-effective device for the development of engineered cardiac tissue,facilitating high-throughput testing in drug development and clinical treatment.HoC is primarily used to create a biomimetic microphysiological environment conducive to fostering the maturation of cardiac tissue and to gather information regarding the real-time condition of cardiac tissue.The development of architectural design and advanced manufacturing for these“3S”components,scaffolds,stimulation,and sensors is essential for improving the maturity of cardiac tissue cultivated on-chip,as well as the precision and accuracy of tissue states.In this review,the typical structures and manufacturing technologies of the“3S”components are summarized.The design and manufacturing suggestions for each component are proposed.Furthermore,key challenges and future perspectives of HoC platforms with integrated“3S”components are discussed.展开更多
Objective To explore the value of epidural injection of hydromorphone for postoperative analgesia after cesarean section.Methods 98 patients with cesarean section in our hospital from April 2019 to April 2020 were sel...Objective To explore the value of epidural injection of hydromorphone for postoperative analgesia after cesarean section.Methods 98 patients with cesarean section in our hospital from April 2019 to April 2020 were selected and divided into observation group and control group according to the order of admission.The control group was given a large dose of0.6 mg hydromorphone,and the observation group was given a small dose of 0.4 mg hydromorphone.The postoperative pain score,postoperative complications,and patients’recognition of analgesia were compared between the two groups.Results The pain scores of the observation group were(2.30±0.45),(2.50±0.33),(2.98±0.73)at 4 h,8 h,and 12 h after operation,which were better than those in the control group.The analgesic satisfaction of the observation group was 93.88%,and that of the control group was 71.43%.The analgesic effect of the observation group was better,and the incidence of postoperative complications was 10.20%in the observation group and 26.52%in the control group.The situation of SAS and SDS in the observation group was better than that in the control group(P<0.05).Conclusion The use of a small dose of 0.4 mg hydromorphone epidural injection,can effectively improve postpartum pain of puerpera,and improve satisfaction of maternal analgesia,while reducing the incidence of postoperative complications of maternal,with clinical research value,worthy of promotion in clinical medicine.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.81971765,31771060,31671025,81871504,32171355 and 82172103).
文摘Currently,stem cell transplantations in cardiac repair are limited owing to disadvantages,such as immunological rejection and poor cell viability.Although direct injection of exosomes can have a curative effect similar to that of stem cell transplantation,high clearance hinders its application in clinical practice.Previous reports suggested that induction of coronary collateralization can be a desired method of adjunctive therapy for someone who had missed the optimal operation time to attenuate myocardial ischemia.In this study,to mimic the paracrine and biological activity of stem cells,we developed artificial stem cells that can continuously release Tβ4-exosomes(Tβ4-ASCs)by encapsulating specific exosomes within microspheres using microfluidics technology.The results show that Tβ4-ASCs can greatly promote coronary collateralization in the periphery of the myocardial infarcted area,and its therapeutic effect is superior to that of directly injecting the exosomes.In addition,to better understand how it works,we demonstrated that the Tβ4-ASC-derived exosomes can enhance the angiogenic capacity of coronary endothelial cells(CAECs)via the miR-17-5p/PHD3/Hif-1αpathway.In brief,as artificial stem cells,Tβ4-ASCs can constantly release functional exosomes and stimulate the formation of collateral circulation after myocardial infarction,providing a feasible and alternative method for clinical revascularization.
基金supported by the National Natural Science Foundation of China(32271423,32000955)the National Key R&D Program of China(2022YFB4600600)the Guangdong Basic and Applied Basic Research Foundation(2024A1515013273).
文摘Organ-on-a-chip stands as a pivotal platform for skeletal muscle research while constructing 3D skeletal muscle tissues that possess both macroscopic and microscopic structures remains a considerable challenge.This study draws inspiration from LEGO-like assembly,employing a modular approach to construct muscle tissue that integrates biomimetic macroscopic and microscopic structures.Modular LEGO-like hybrid nanofibrous scaffold bricks were fabricated by the combination of 3D printing and electrospinning techniques.Skeletal muscle cells cultured on these modular scaffold bricks exhibited a highly orientated nanofibrous structure.A variety of construction of skeletal muscle tissues further enabled development by various assembling processes.Moreover,skeletal muscle-on-a-chip(SMoC)was further assembled as a functional platform for electrical or perfusion stimuli investigation.The electrical stimulus was conveniently applied and tuned in such a SMoC platform to significantly enhance the differentiation of skeletal muscle tissues.Additionally,the effect of perfusion stimulation on skeletal muscle vascularization within the SMoC platform was also demonstrated.These findings highlight the potential of these assembled SMoCs as functional ex vivo platforms for skeletal tissue engineering and drug research applications,and such a LEGO-like assembly strategy could also be applied to the other engineering organ-on-chips fabrication,which facilitates the development of bionic functional platforms for various biomedical research applications.
基金supported by the National Natural Science Foundation of China(No.U2005214)the National Key Research and Development Program of China(2022YFB4600600).
文摘Heart-on-a-chip(HoC)has emerged as a highly efficient,cost-effective device for the development of engineered cardiac tissue,facilitating high-throughput testing in drug development and clinical treatment.HoC is primarily used to create a biomimetic microphysiological environment conducive to fostering the maturation of cardiac tissue and to gather information regarding the real-time condition of cardiac tissue.The development of architectural design and advanced manufacturing for these“3S”components,scaffolds,stimulation,and sensors is essential for improving the maturity of cardiac tissue cultivated on-chip,as well as the precision and accuracy of tissue states.In this review,the typical structures and manufacturing technologies of the“3S”components are summarized.The design and manufacturing suggestions for each component are proposed.Furthermore,key challenges and future perspectives of HoC platforms with integrated“3S”components are discussed.
文摘Objective To explore the value of epidural injection of hydromorphone for postoperative analgesia after cesarean section.Methods 98 patients with cesarean section in our hospital from April 2019 to April 2020 were selected and divided into observation group and control group according to the order of admission.The control group was given a large dose of0.6 mg hydromorphone,and the observation group was given a small dose of 0.4 mg hydromorphone.The postoperative pain score,postoperative complications,and patients’recognition of analgesia were compared between the two groups.Results The pain scores of the observation group were(2.30±0.45),(2.50±0.33),(2.98±0.73)at 4 h,8 h,and 12 h after operation,which were better than those in the control group.The analgesic satisfaction of the observation group was 93.88%,and that of the control group was 71.43%.The analgesic effect of the observation group was better,and the incidence of postoperative complications was 10.20%in the observation group and 26.52%in the control group.The situation of SAS and SDS in the observation group was better than that in the control group(P<0.05).Conclusion The use of a small dose of 0.4 mg hydromorphone epidural injection,can effectively improve postpartum pain of puerpera,and improve satisfaction of maternal analgesia,while reducing the incidence of postoperative complications of maternal,with clinical research value,worthy of promotion in clinical medicine.
