AIM:To elucidate the profiles of commensal bacteria on the ocular surfaces of patients with varying severity of dry eye(DE).METHODS:The single-center,prospective,case-control,observational study categorized all partic...AIM:To elucidate the profiles of commensal bacteria on the ocular surfaces of patients with varying severity of dry eye(DE).METHODS:The single-center,prospective,case-control,observational study categorized all participants into three distinct groups:1)control group(n=61),2)mild DE group(n=56),and 3)moderate-to-severe DE group(n=82).Schirmer’s tear secretion strips were used,and the bacterial microbiota was analyzed using 16S ribosomal ribonucleic acid gene sequencing.RESULTS:The three groups had significant differences in alpha diversity:the control group had the highest richness(Chao1,Faith’s phylogenetic diversity),the mild DE group showed the highest diversity(Shannon,Simpson),and the moderate-to-severe DE group had the lowest of the above-mentioned indices.DE severity was positively correlated with a reduction in beta diversity of the microbial community,with the moderate-to-severe DE group exhibiting the lowest beta diversity.Linear discriminant analysis effect size presented distinct dominant taxa that significantly differed between each.Furthermore,the exacerbation of DE corresponded with the enrichment of certain pathogenic bacteria,as determined by random forest analysis.CONCLUSION:As DE severity worsens,microbial community diversity tends to decrease.DE development corresponds with changes in microbial constituents,primarily characterized by reduced microbial diversity and a more homogenous species composition.展开更多
随着电动汽车渗透率的高速增长,不合理的充换电站比例规划会导致配电网电能质量和运行经济性下降,亟需建立配电网电能质量指标体系对充换电站的比例规划进行评估。传统指标体系赋权方式单一,且不能准确描述指标间相互关系。文中提出了...随着电动汽车渗透率的高速增长,不合理的充换电站比例规划会导致配电网电能质量和运行经济性下降,亟需建立配电网电能质量指标体系对充换电站的比例规划进行评估。传统指标体系赋权方式单一,且不能准确描述指标间相互关系。文中提出了基于改进序关系分析(G1-criteria importance though intercrieria correlation,CRITIC)组合赋权针对不同比例充换电站规划的配电网电能质量评价方法。首先,从配电网电压越限率及稳定裕度、馈线均衡度及损耗、电流谐波畸变率多个角度设立可量化指标,建立配电网电能质量指标体系;其次,采用Kendall相关系数对CRITIC客观赋权法进行改进,利用主客观组合赋权法对体系内各个指标进行赋权;然后,通过赋权后的指标体系对不同职能区域进行充换电站比例规划评估。最后,通过仿真实例分析,验证了所提配电网电能质量指标体系的合理性以及改进后赋权方法的有效性。展开更多
为了提高甘露聚糖酶ManA在毕赤酵母中分泌表达的酶活,选择毕赤酵母内质网未折叠蛋白反应(Unfolded protein response,UPR)激活调控因子HAC1与5种毕赤酵母蛋白折叠相关的分子伴侣ERO1、PDI、PDI1、CPR5、BiP,通过构建pPICZA-HAC1等6种胞...为了提高甘露聚糖酶ManA在毕赤酵母中分泌表达的酶活,选择毕赤酵母内质网未折叠蛋白反应(Unfolded protein response,UPR)激活调控因子HAC1与5种毕赤酵母蛋白折叠相关的分子伴侣ERO1、PDI、PDI1、CPR5、BiP,通过构建pPICZA-HAC1等6种胞内表达重组质粒,分别电转化至分泌表达ManA的毕赤酵母重组菌中胞内共表达,并分析其重组菌摇瓶发酵时ManA表达的影响。结果发现在摇瓶发酵水平,胞内共表达HAC1、ERO1、PDI的重组菌发酵上清液中的ManA酶活力分别提高了26%、15%、20%,其重组菌发酵上清液的酶活力分别达到1 014 U/mL、925 U/mL、965 U/mL。通过对各重组菌上清液酶活力、胞内滞留酶活力、上清液蛋白浓度数据进行分析,进一步选择将HAC1、ERO1、PDI进行两基因或三基因组合,并分别在分泌表达ManA的重组菌胞内共表达,但各共表达重组菌发酵上清液的酶活力都没有进一步的提升。单独共表达HAC1或者分子伴侣ERO1、PDI可以辅助ManA的正确折叠,提高其蛋白表达。展开更多
随着电力系统中新能源机组渗透率的快速提高,系统惯量水平下降将威胁系统频率稳定性,惯量的空间分布特征也将更加凸显,频率响应的分散性将不能被忽略。针对以上问题,提出一种考虑频率响应分散性及系统分区的含风电电力系统等效惯量估计...随着电力系统中新能源机组渗透率的快速提高,系统惯量水平下降将威胁系统频率稳定性,惯量的空间分布特征也将更加凸显,频率响应的分散性将不能被忽略。针对以上问题,提出一种考虑频率响应分散性及系统分区的含风电电力系统等效惯量估计方法。首先,为降低频率响应分散性对估计精度的影响,基于谱聚类算法对电力系统进行分区,并基于皮尔逊相关系数定义频率相似度指标确定区域频率的最优测量节点。其次,由于测量所得的频率变化率(rate of change of frequency, RoCoF)曲线中包含大量的振荡分量,提出一种基于摇摆方程的数值积分方法估计区域及系统全局惯量。最后,在DIgSILENT/PowerFactory中建立改进IEEE10机39节点系统以验证所提方法的有效性。仿真结果表明,该方法适用于不同场景下含风电系统的等效惯量估计。展开更多
基金Supported by Shanghai Municipal Health Commission(No.201940243).
文摘AIM:To elucidate the profiles of commensal bacteria on the ocular surfaces of patients with varying severity of dry eye(DE).METHODS:The single-center,prospective,case-control,observational study categorized all participants into three distinct groups:1)control group(n=61),2)mild DE group(n=56),and 3)moderate-to-severe DE group(n=82).Schirmer’s tear secretion strips were used,and the bacterial microbiota was analyzed using 16S ribosomal ribonucleic acid gene sequencing.RESULTS:The three groups had significant differences in alpha diversity:the control group had the highest richness(Chao1,Faith’s phylogenetic diversity),the mild DE group showed the highest diversity(Shannon,Simpson),and the moderate-to-severe DE group had the lowest of the above-mentioned indices.DE severity was positively correlated with a reduction in beta diversity of the microbial community,with the moderate-to-severe DE group exhibiting the lowest beta diversity.Linear discriminant analysis effect size presented distinct dominant taxa that significantly differed between each.Furthermore,the exacerbation of DE corresponded with the enrichment of certain pathogenic bacteria,as determined by random forest analysis.CONCLUSION:As DE severity worsens,microbial community diversity tends to decrease.DE development corresponds with changes in microbial constituents,primarily characterized by reduced microbial diversity and a more homogenous species composition.
文摘随着电动汽车渗透率的高速增长,不合理的充换电站比例规划会导致配电网电能质量和运行经济性下降,亟需建立配电网电能质量指标体系对充换电站的比例规划进行评估。传统指标体系赋权方式单一,且不能准确描述指标间相互关系。文中提出了基于改进序关系分析(G1-criteria importance though intercrieria correlation,CRITIC)组合赋权针对不同比例充换电站规划的配电网电能质量评价方法。首先,从配电网电压越限率及稳定裕度、馈线均衡度及损耗、电流谐波畸变率多个角度设立可量化指标,建立配电网电能质量指标体系;其次,采用Kendall相关系数对CRITIC客观赋权法进行改进,利用主客观组合赋权法对体系内各个指标进行赋权;然后,通过赋权后的指标体系对不同职能区域进行充换电站比例规划评估。最后,通过仿真实例分析,验证了所提配电网电能质量指标体系的合理性以及改进后赋权方法的有效性。
文摘随着电力系统中新能源机组渗透率的快速提高,系统惯量水平下降将威胁系统频率稳定性,惯量的空间分布特征也将更加凸显,频率响应的分散性将不能被忽略。针对以上问题,提出一种考虑频率响应分散性及系统分区的含风电电力系统等效惯量估计方法。首先,为降低频率响应分散性对估计精度的影响,基于谱聚类算法对电力系统进行分区,并基于皮尔逊相关系数定义频率相似度指标确定区域频率的最优测量节点。其次,由于测量所得的频率变化率(rate of change of frequency, RoCoF)曲线中包含大量的振荡分量,提出一种基于摇摆方程的数值积分方法估计区域及系统全局惯量。最后,在DIgSILENT/PowerFactory中建立改进IEEE10机39节点系统以验证所提方法的有效性。仿真结果表明,该方法适用于不同场景下含风电系统的等效惯量估计。
