Diacylglycerols(DAGs),which are involved in rapid energy supply and fat reduction,are typically produced from triglycerides,glycerol and fatty acids through lipase-catalysis.This study introduces a novel method for pr...Diacylglycerols(DAGs),which are involved in rapid energy supply and fat reduction,are typically produced from triglycerides,glycerol and fatty acids through lipase-catalysis.This study introduces a novel method for preparing DAGs in a solvent-free system by using phospholipase C(PLC)hydrolysis of rapeseed oil sediment,which is a byproduct of the rapeseed oil water degumming.The optimisation of DAGs production by PLC hydrolysis using Response Surface Methodology(RMS)and Box-Behnken design.Under optimal conditions of 0.6% enzyme concentration,51℃ reaction temperature,56% water content and 2.2 h reaction time,phosphatidylcholine(PC)and phosphatidylethanolamine in the oil sediment were almost entirely converted into DAGs.This reaction yielded an oil product containing 27.1%DAGs,with 1,2-DAGs at 24.88%±0.01% and 1,3-DAGs at 2.22%±0.01%,which is favoured in drug delivery systems,and the fatty acid composition of DAGs oil was almost consistent with rapeseed oil.Moreover,the residual phospholipids were primarily phosphatidylinositol(PI),with an amount of 69.73%,suggesting the potential for high-purity PI production.In this study,rapeseed oil sediment achieved comprehensive utilization through hydrolysis by PLC,which not only enhanced the value of the oil sediment but also provided a new approach for the synthesis of DAGs.展开更多
基金supported by the“National Key Research and Development Program of China(Grant number:2021YFD1600500)”“Special Project of Central Guide to Local Science and Technology Development(Innovation platform construction for food green processing technology and intelligent equipment)(Grant number:2022BGE247)”.
文摘Diacylglycerols(DAGs),which are involved in rapid energy supply and fat reduction,are typically produced from triglycerides,glycerol and fatty acids through lipase-catalysis.This study introduces a novel method for preparing DAGs in a solvent-free system by using phospholipase C(PLC)hydrolysis of rapeseed oil sediment,which is a byproduct of the rapeseed oil water degumming.The optimisation of DAGs production by PLC hydrolysis using Response Surface Methodology(RMS)and Box-Behnken design.Under optimal conditions of 0.6% enzyme concentration,51℃ reaction temperature,56% water content and 2.2 h reaction time,phosphatidylcholine(PC)and phosphatidylethanolamine in the oil sediment were almost entirely converted into DAGs.This reaction yielded an oil product containing 27.1%DAGs,with 1,2-DAGs at 24.88%±0.01% and 1,3-DAGs at 2.22%±0.01%,which is favoured in drug delivery systems,and the fatty acid composition of DAGs oil was almost consistent with rapeseed oil.Moreover,the residual phospholipids were primarily phosphatidylinositol(PI),with an amount of 69.73%,suggesting the potential for high-purity PI production.In this study,rapeseed oil sediment achieved comprehensive utilization through hydrolysis by PLC,which not only enhanced the value of the oil sediment but also provided a new approach for the synthesis of DAGs.
