Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of ...Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of apoptosis and myocarditis,and miR-219a-5p has been found to suppress the inflammatory response.However,unknown is the precise mechanism by which miR-219a-5p contributes to MI.Methods:We measured the expression of miR-219a-5p and evaluated its effects on target proteins,inflammatory factors,and apoptosis in a mouse model of MI.Echocardiography was utilized to examine the MI clinical index,and triphenyl tetrazolium chloride staining was employed to analyze the infarcted region.Enzyme-linked immunosorbent assay and Western blotting measured serum and molecular markers in heart tissues.To quantify the association with miR-219a-5p and ATPase sarcoplasmic/endoplasmic reticulum Ca^(2+) transporting 2(ATP2A2),the luciferase activity assay and Pearson’s correlation analysis were employed.Results:MiR-219a-5p exhibited low expression in a mouse model of MI,and its amplification prevented both apoptotic and inflammatory reactions.Specifically,miR-219a-5p targeted ATP2A2.Conclusion:In a mouse model of MI,miR-219a-5p exerted a potent protective effect via direct targeting of ATP2A2.展开更多
Metal-organic frameworks(MOFs)materials exhibit inherent advantages in microwave absorption(MA)due to their unique compositional and structural characteristics.However,it remains a significant challenge to systematica...Metal-organic frameworks(MOFs)materials exhibit inherent advantages in microwave absorption(MA)due to their unique compositional and structural characteristics.However,it remains a significant challenge to systematically elucidate the coordinated effects of component modulation and morphology design on electromagnetic parameters during the complex modification process to enhance the MA performance of MOFs.In this study,the twodimensional(2D)Co-based MOFs material,layered zeolitic imidazolate framework(ZIF-L),was meticulously selected as a structure design template and combined with an efficient ion-exchange component modulation method,aiming to stimulate the potential advantages between the two in the synergistic modulation of electromagnetic parameters.Specifically,the ion-exchange method enhanced various polarization mechanisms,including interface,dipole,and defect-induced polarizations.Meanwhile,the 2D template facilitates the formation of a robust conductive network on the surface and ensures the high efficiency and uniformity of the ion-exchange process.Ultimately,the resulting material achieved an adequate absorption bandwidth(EAB)of 6.47 GHz at a thickness of 2 mm,a 78% improvement over the untreated sample(EAB=3.64 GHz).This research not only provides valuable theoretical insights into the application of 2D MOFs materials in MA but also offers innovative perspectives for the design and development of energy-saving and environmentally friendly microwave absorption materials(MAMs).展开更多
基金supported by the National Nature Science Foundation of the People’s Republic of China(No.81400225 for Zulong Sheng and No.82000382 for Yanru He)the Jiangsu Provincial Medical Youth Talent(No.QNRC2016815).
文摘Background:Myocardial infarction(MI)is known worldwide for its important disabling features,including myocarditis and cardiomyocyte apoptosis.It is believed that microRNA(miRNA)has a role in the cellular processes of apoptosis and myocarditis,and miR-219a-5p has been found to suppress the inflammatory response.However,unknown is the precise mechanism by which miR-219a-5p contributes to MI.Methods:We measured the expression of miR-219a-5p and evaluated its effects on target proteins,inflammatory factors,and apoptosis in a mouse model of MI.Echocardiography was utilized to examine the MI clinical index,and triphenyl tetrazolium chloride staining was employed to analyze the infarcted region.Enzyme-linked immunosorbent assay and Western blotting measured serum and molecular markers in heart tissues.To quantify the association with miR-219a-5p and ATPase sarcoplasmic/endoplasmic reticulum Ca^(2+) transporting 2(ATP2A2),the luciferase activity assay and Pearson’s correlation analysis were employed.Results:MiR-219a-5p exhibited low expression in a mouse model of MI,and its amplification prevented both apoptotic and inflammatory reactions.Specifically,miR-219a-5p targeted ATP2A2.Conclusion:In a mouse model of MI,miR-219a-5p exerted a potent protective effect via direct targeting of ATP2A2.
基金supported by the National Natural Science Foundation of China(No.52172295)Young talent lift project(No.2021-JCJQQT-064)+1 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX24_0571)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX23_0371).
文摘Metal-organic frameworks(MOFs)materials exhibit inherent advantages in microwave absorption(MA)due to their unique compositional and structural characteristics.However,it remains a significant challenge to systematically elucidate the coordinated effects of component modulation and morphology design on electromagnetic parameters during the complex modification process to enhance the MA performance of MOFs.In this study,the twodimensional(2D)Co-based MOFs material,layered zeolitic imidazolate framework(ZIF-L),was meticulously selected as a structure design template and combined with an efficient ion-exchange component modulation method,aiming to stimulate the potential advantages between the two in the synergistic modulation of electromagnetic parameters.Specifically,the ion-exchange method enhanced various polarization mechanisms,including interface,dipole,and defect-induced polarizations.Meanwhile,the 2D template facilitates the formation of a robust conductive network on the surface and ensures the high efficiency and uniformity of the ion-exchange process.Ultimately,the resulting material achieved an adequate absorption bandwidth(EAB)of 6.47 GHz at a thickness of 2 mm,a 78% improvement over the untreated sample(EAB=3.64 GHz).This research not only provides valuable theoretical insights into the application of 2D MOFs materials in MA but also offers innovative perspectives for the design and development of energy-saving and environmentally friendly microwave absorption materials(MAMs).
