Increased matrix stiffness of nucleus pulposus(NP)tissue is a main feature of intervertebral disc degeneration(IVDD)and affects various functions of nucleus pulposus cells(NPCs).Glycolysis is the main energy source fo...Increased matrix stiffness of nucleus pulposus(NP)tissue is a main feature of intervertebral disc degeneration(IVDD)and affects various functions of nucleus pulposus cells(NPCs).Glycolysis is the main energy source for NPC survival,but the effects and underlying mechanisms of increased extracellular matrix(ECM)stiffness on NPC glycolysis remain unknown.In this study,hydrogels with different stiffness were established to mimic the mechanical environment of NPCs.Notably,increased matrix stiffness in degenerated NP tissues from IVDD patients was accompanied with impaired glycolysis,and NPCs cultured on rigid substrates exhibited a reduction in glycolysis.展开更多
Super high-rise buildings are typically located in bustling urban areas.Due to their considerable height and limited construction space,numerous challenges arise during the construction process.To enhance the safety a...Super high-rise buildings are typically located in bustling urban areas.Due to their considerable height and limited construction space,numerous challenges arise during the construction process.To enhance the safety and efficiency of super highrise building construction,this paper designs an intelligent construction machine steel truss platform suitable for the core tube structure of such buildings.Numerical analyses of the designed steel truss platform were conducted during the construction,jacking,and self-climbing phases.The analysis results indicate that the strength and stiffness of the intelligent construction machine steel truss platform meet the design standards for steel structures,ensuring the overall safety and reliability of the structure.However,stress and deformation are relatively high at the connections between primary and secondary trusses,particularly in the chords and webs,as well as at the joints between truss beams and columns.To facilitate construction and improve structural safety reserves,technical and structural optimization suggestions are proposed based on the analysis results.This study provides valuable insights for the design of similar intelligent construction machine steel truss platforms in engineering projects.展开更多
基金supported by the National Nature Science Foundation of China(No.82002345 to J.D and 81902179 to L.S)the Gusu Talent Program(No.Qngg2022008 and GSWS2021027 to J.D)the Preliminary Research Project of the Second Affiliated Hospital of Soochow University(No.SDFEYBS1905 to J.D).
文摘Increased matrix stiffness of nucleus pulposus(NP)tissue is a main feature of intervertebral disc degeneration(IVDD)and affects various functions of nucleus pulposus cells(NPCs).Glycolysis is the main energy source for NPC survival,but the effects and underlying mechanisms of increased extracellular matrix(ECM)stiffness on NPC glycolysis remain unknown.In this study,hydrogels with different stiffness were established to mimic the mechanical environment of NPCs.Notably,increased matrix stiffness in degenerated NP tissues from IVDD patients was accompanied with impaired glycolysis,and NPCs cultured on rigid substrates exhibited a reduction in glycolysis.
基金Shaanxi Provincial Key Research and Development Program(2023JBGS-17)。
文摘Super high-rise buildings are typically located in bustling urban areas.Due to their considerable height and limited construction space,numerous challenges arise during the construction process.To enhance the safety and efficiency of super highrise building construction,this paper designs an intelligent construction machine steel truss platform suitable for the core tube structure of such buildings.Numerical analyses of the designed steel truss platform were conducted during the construction,jacking,and self-climbing phases.The analysis results indicate that the strength and stiffness of the intelligent construction machine steel truss platform meet the design standards for steel structures,ensuring the overall safety and reliability of the structure.However,stress and deformation are relatively high at the connections between primary and secondary trusses,particularly in the chords and webs,as well as at the joints between truss beams and columns.To facilitate construction and improve structural safety reserves,technical and structural optimization suggestions are proposed based on the analysis results.This study provides valuable insights for the design of similar intelligent construction machine steel truss platforms in engineering projects.
