The development of pattern-based traditional Chinese medicine(TCM)compound preparations constitutes a core domain that represents the principle of pattern differentiation-based treatment,a hallmark of TCM.However,the ...The development of pattern-based traditional Chinese medicine(TCM)compound preparations constitutes a core domain that represents the principle of pattern differentiation-based treatment,a hallmark of TCM.However,the field has long been constrained by scientific and regulatory challenges in animal modeling,efficacy evaluation,and clinical positioning.This article proposes a new research and development(R&D)paradigm strategically based on human use experience(HUE)and centered on the patient as the key to overcoming these bottlenecks and achieving high-quality progress.We systematically dissect the traditional problems in this field and demonstrate the pivotal role of high-quality HUE in enabling precise clinical positioning and optimizing R&D pathways(e.g.,applying for exemptions from non-clinical studies).HUE guides the implementation of the“pattern-symptom integration”model.Furthermore,we detail the implementation of the patient-centered concept throughout the process of clinical trial design,collection of patients’experience data,clinical outcome assessment,and benefit-risk assessment.The integrative application of artificial intelligence in the R&D of pattern-based TCM drugs is also specifically explored.By synthesizing the“TCM theory,HUE,and clinical trials”evidence system,this article aims to provide a systematic strategic framework for establishing an R&D pathway that adheres to the intrinsic principles of TCM while simultaneously meeting modern scientific standards.展开更多
Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enh...Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gill and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.展开更多
High-frequency(HF)and ultrahigh-frequency(UHF)dual-band radio frequency identification(RFID)tags with both near-field and farfield communication can meet different application scenarios.However,it is time-consuming to...High-frequency(HF)and ultrahigh-frequency(UHF)dual-band radio frequency identification(RFID)tags with both near-field and farfield communication can meet different application scenarios.However,it is time-consuming to calculate the return loss of a UHF antenna in a dualband tag antenna using electromagnetic(EM)simulators.To overcome this,the present work proposes a model of a multi-scale convolutional neural network stacked with long and short-term memory(MSCNN-LSTM)for predicting the return loss of UHF antennas instead of EM simulators.In the proposed MSCNN-LSTM,the MSCNN has three branches,which include three convolution layers with different kernel sizes and numbers.Therefore,MSCNN can extract fine-grain localized information of the antenna and overall features.The LSTM can effectively learn the EM characteristics of different structures of the antenna to improve the prediction accuracy of the model.Experimental results show that the mean absolute error(0.0073),mean square error(0.00032),and root mean square error(0.01814)of theMSCNNLSTM are better than those of other prediction methods.In predicting the return loss of 100UHFantennas,compared with the simulation time of 4800 s for High Frequency Structure Simulator(HFSS),MSCNN-LSTM takes only 0.927519 s under the premise of ensuring prediction accuracy,significantly reducing the calculation time,which provides a basis for the rapid design of HF-UHF RFID tag antenna.ThenMSCNN-LSTM is used to determine the dimensions of the UHF antenna quickly.The return loss of the designed dualband RFID tag antenna is−58.76 and−22.63 dB at 13.56 and 915 MHz,respectively,achieving the desired goal.展开更多
Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophy...Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.展开更多
By using the method of reverse transcription at different dNTP concentrations, a novel methylated nucleoside, Am64, has been identified from Schizosaccharomyces pombe U6 snRNA, and a higher ordered structure repressin...By using the method of reverse transcription at different dNTP concentrations, a novel methylated nucleoside, Am64, has been identified from Schizosaccharomyces pombe U6 snRNA, and a higher ordered structure repressing the passage of reverse transcriptase at the 5’ end of U6 snRNA has been demonstrated.展开更多
基金supported by the Key Laboratory of Clinical Evaluation Technology for Human Experience with Chinese Medicine,Science and Technology Innovation Project of Guangdong Provincial Medical Products Administration(2022ZDB06)the Jointly Funded Project by Guangzhou Science and Technology Bureau,Research Institutes and Enterprises(2024A03j0355)+1 种基金the State Key Laboratory of Traditional Chinese Medicine Syndrome"Unveiling the List and Taking on Leadership"Project(SKLKY2025C0013)the National Key Research and Development Program of China(2025YFC3507902).
文摘The development of pattern-based traditional Chinese medicine(TCM)compound preparations constitutes a core domain that represents the principle of pattern differentiation-based treatment,a hallmark of TCM.However,the field has long been constrained by scientific and regulatory challenges in animal modeling,efficacy evaluation,and clinical positioning.This article proposes a new research and development(R&D)paradigm strategically based on human use experience(HUE)and centered on the patient as the key to overcoming these bottlenecks and achieving high-quality progress.We systematically dissect the traditional problems in this field and demonstrate the pivotal role of high-quality HUE in enabling precise clinical positioning and optimizing R&D pathways(e.g.,applying for exemptions from non-clinical studies).HUE guides the implementation of the“pattern-symptom integration”model.Furthermore,we detail the implementation of the patient-centered concept throughout the process of clinical trial design,collection of patients’experience data,clinical outcome assessment,and benefit-risk assessment.The integrative application of artificial intelligence in the R&D of pattern-based TCM drugs is also specifically explored.By synthesizing the“TCM theory,HUE,and clinical trials”evidence system,this article aims to provide a systematic strategic framework for establishing an R&D pathway that adheres to the intrinsic principles of TCM while simultaneously meeting modern scientific standards.
基金funded by the National Natural Science Foundation of China,No.81171401Science and Technology Development Program of Dalian City,No.2008J99JH268the Scientific Research Program of Higher Learning School of Department of Education of Liaoning Province,No.L20100108
文摘Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gill and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.
基金The research work is carried out under the Beijing Natural Science Foundation-Beijing Education Commission Joint Project(KZ202210015020)Discipline Construction and Postgraduate Education Project of BIGC(No.21090122005)BIGC Project(Ee202204).
文摘High-frequency(HF)and ultrahigh-frequency(UHF)dual-band radio frequency identification(RFID)tags with both near-field and farfield communication can meet different application scenarios.However,it is time-consuming to calculate the return loss of a UHF antenna in a dualband tag antenna using electromagnetic(EM)simulators.To overcome this,the present work proposes a model of a multi-scale convolutional neural network stacked with long and short-term memory(MSCNN-LSTM)for predicting the return loss of UHF antennas instead of EM simulators.In the proposed MSCNN-LSTM,the MSCNN has three branches,which include three convolution layers with different kernel sizes and numbers.Therefore,MSCNN can extract fine-grain localized information of the antenna and overall features.The LSTM can effectively learn the EM characteristics of different structures of the antenna to improve the prediction accuracy of the model.Experimental results show that the mean absolute error(0.0073),mean square error(0.00032),and root mean square error(0.01814)of theMSCNNLSTM are better than those of other prediction methods.In predicting the return loss of 100UHFantennas,compared with the simulation time of 4800 s for High Frequency Structure Simulator(HFSS),MSCNN-LSTM takes only 0.927519 s under the premise of ensuring prediction accuracy,significantly reducing the calculation time,which provides a basis for the rapid design of HF-UHF RFID tag antenna.ThenMSCNN-LSTM is used to determine the dimensions of the UHF antenna quickly.The return loss of the designed dualband RFID tag antenna is−58.76 and−22.63 dB at 13.56 and 915 MHz,respectively,achieving the desired goal.
基金Ningxia Natural Science Foundation(NZ15270)National Agriculture Research System of Oat and Buckwheat(CARS-08-E-5).
文摘Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.
文摘By using the method of reverse transcription at different dNTP concentrations, a novel methylated nucleoside, Am64, has been identified from Schizosaccharomyces pombe U6 snRNA, and a higher ordered structure repressing the passage of reverse transcriptase at the 5’ end of U6 snRNA has been demonstrated.
