Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield.However,the molecular mechanism of linseed response to salt stress is still unclear.In this study,a sa...Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield.However,the molecular mechanism of linseed response to salt stress is still unclear.In this study,a salt-tolerant(ST)linseed variety STS and a salt-sensitive(SS)variety DYM were selected as experiment materials.Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci(QTL).A total of 38,625 QTL loci were identified.Fifteen genes(which were not annotated in the reference genome)were identified within a 2.597 Mb region in chromosome 1.Two salt tolerance candidate genes Lus.o.m.scaffold91.141 and Lus.o.m.Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions.Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance.In this paper,results showed that Lus.o.m.scaffold91.141 and Lus.o.m.Scaffold1.14 might be involved in response to salt stress in lineseed.The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties.展开更多
Crop yield is primarily seed-filling-limited in production system under field conditions.This study was aimed to determine whether seed filling traits of oil flax(Linum usitatissimum L.)could be controlled by phosphor...Crop yield is primarily seed-filling-limited in production system under field conditions.This study was aimed to determine whether seed filling traits of oil flax(Linum usitatissimum L.)could be controlled by phosphorus(P),nitrogen(N),and phosphorus and nitrogen(NP)supply.Effects on seed filling traits were investigated in 2 years including capsule diameter,capsule height,capsule dry matter(DM),seed DM per capsule,pericarp DM per capsule,protein content and oil content.DM translocation from pericarps to seed,translocation efficiency,and contribution of photoassimilates during seed filling period were also detected.In a randomized complete block design,cultivar'Longyaza 1'was grown under P(33 kg P/ha),N(75 kg N/ha),and NP(33 kg P/ha and 75 kg N/ha)along with a zerofertilizer(CK)treatment in 2013 and 2014.Results suggested that DM translocation efficiency and contribution efficiency increased to different extent due to P,N or NP application.At 42 DAA(days after anthesis),seed DM per capsule reached the greatest,while protein content and pericarp DM obtained the least level.However,the highest oil content was detected at 35 DAA.A significant positive linear relationship was observed between seed DM,capsule DM and DM translocation in both years.Protein content showed inconsistent relation with oil content.The results indicated that appropriate N and P management could be an effective approach to increase oil flax production.展开更多
The secoisolariciresinol diglycoside(SDG)lignan of oil flax(Linum usitatissimum L.)is influenced by genotype,environment and the interaction of genotype×environment.This field study was conducted to investigate t...The secoisolariciresinol diglycoside(SDG)lignan of oil flax(Linum usitatissimum L.)is influenced by genotype,environment and the interaction of genotype×environment.This field study was conducted to investigate the effects of genotype and environment on the SDG lignan content,six genotypes(Longya 8,Zhangya 2,Linxiabai,Shandanbai,Gaolanbai,and DYMS)were sown under eight locations(Hohhot,Datong,Minle,Yili,Shuangta,Jingtai,Guyuan,and Zhangjiakou)in 2014 and 2015 in China.The experimental design was a randomized complete block with three replications.Results showed that Longya 8 had the highest SDG lignan content(7.27mg/g)and Shandanbai had the lowest(4.71 mg/g)across 16 environments.The SDG lignan content ranged from 4.70 mg/g at Datong to 7.74 mg/g at Minle Research Station.In the present study,the results indicated that differences among genotypes in SDG lignan content accounted for 51.38%variation of the total variation and environments accounted for 44.40%variation of the total variation.Moreover,we found that the altitude had a positive effect on SDG lignan content of flaxseed,presumably owing to differences in temperature,humidity,sunshine time,etc.These results indicated that the SDG lignan content was genetically controlled,and was also to be influenced by environmental conditions,especially altitude.展开更多
基金financially supported by grants from the National Natural Science Foundation of China (NSFC) (Grant no. 31560347, and 31760426)the China Agriculture Research System (CARS-14)
文摘Soil salinization is detrimental to the growth and development of flax and ultimately leads to a decrease in yield.However,the molecular mechanism of linseed response to salt stress is still unclear.In this study,a salt-tolerant(ST)linseed variety STS and a salt-sensitive(SS)variety DYM were selected as experiment materials.Bulk segregation analysis and whole-genome resequencing technologies were performed to map salt tolerance quantitative trait loci(QTL).A total of 38,625 QTL loci were identified.Fifteen genes(which were not annotated in the reference genome)were identified within a 2.597 Mb region in chromosome 1.Two salt tolerance candidate genes Lus.o.m.scaffold91.141 and Lus.o.m.Scaffold1.14 encoding WD40 and cytochrome P450 were identified by predicting protein functions.Previous studies showed that WD40 and cytochrome P450 could significantly improve plant salt stress tolerance.In this paper,results showed that Lus.o.m.scaffold91.141 and Lus.o.m.Scaffold1.14 might be involved in response to salt stress in lineseed.The fine mapping and functional analysis of these genes provide a molecular breeding basis for the genetic improvement of high salt-tolerant linseed varieties.
基金supported by the National Natural Science Foundation of China (31660368, 31560347 and 31360315)the Technology Innovation of Oil Crops Breeding and Innovation Team of New Cultivars Breeding (2017GAAS22)+1 种基金the Science and Technology Plan Project of Gansu Province (17ZD2NA016-3)the China Agriculture Research System of Construct Special (CARS-17-GW04, CARS-17-GW-09)
文摘Crop yield is primarily seed-filling-limited in production system under field conditions.This study was aimed to determine whether seed filling traits of oil flax(Linum usitatissimum L.)could be controlled by phosphorus(P),nitrogen(N),and phosphorus and nitrogen(NP)supply.Effects on seed filling traits were investigated in 2 years including capsule diameter,capsule height,capsule dry matter(DM),seed DM per capsule,pericarp DM per capsule,protein content and oil content.DM translocation from pericarps to seed,translocation efficiency,and contribution of photoassimilates during seed filling period were also detected.In a randomized complete block design,cultivar'Longyaza 1'was grown under P(33 kg P/ha),N(75 kg N/ha),and NP(33 kg P/ha and 75 kg N/ha)along with a zerofertilizer(CK)treatment in 2013 and 2014.Results suggested that DM translocation efficiency and contribution efficiency increased to different extent due to P,N or NP application.At 42 DAA(days after anthesis),seed DM per capsule reached the greatest,while protein content and pericarp DM obtained the least level.However,the highest oil content was detected at 35 DAA.A significant positive linear relationship was observed between seed DM,capsule DM and DM translocation in both years.Protein content showed inconsistent relation with oil content.The results indicated that appropriate N and P management could be an effective approach to increase oil flax production.
基金the China Agriculture Research System of MOF and MARA,Technology Innovation Project of Gansu Academy of Agricultural Sciences(2021GAAS20)the Technology Innovation of Oil Crops Molecular Breeding and Application(2020GAAS08)。
文摘The secoisolariciresinol diglycoside(SDG)lignan of oil flax(Linum usitatissimum L.)is influenced by genotype,environment and the interaction of genotype×environment.This field study was conducted to investigate the effects of genotype and environment on the SDG lignan content,six genotypes(Longya 8,Zhangya 2,Linxiabai,Shandanbai,Gaolanbai,and DYMS)were sown under eight locations(Hohhot,Datong,Minle,Yili,Shuangta,Jingtai,Guyuan,and Zhangjiakou)in 2014 and 2015 in China.The experimental design was a randomized complete block with three replications.Results showed that Longya 8 had the highest SDG lignan content(7.27mg/g)and Shandanbai had the lowest(4.71 mg/g)across 16 environments.The SDG lignan content ranged from 4.70 mg/g at Datong to 7.74 mg/g at Minle Research Station.In the present study,the results indicated that differences among genotypes in SDG lignan content accounted for 51.38%variation of the total variation and environments accounted for 44.40%variation of the total variation.Moreover,we found that the altitude had a positive effect on SDG lignan content of flaxseed,presumably owing to differences in temperature,humidity,sunshine time,etc.These results indicated that the SDG lignan content was genetically controlled,and was also to be influenced by environmental conditions,especially altitude.
