The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are bar...The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are barely understood.This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells.The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL,which did not cause disorder in the normal cell metabolism and absorption activity.The Caco-2 cell monolayer model could absorb DBP by passive and active transport,and the absorptive pattern was dosedependent when the concentration was more than 0.25 mg/mL.During DBP absorption,an increase in m RNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP.But the glucose transport amounts were significantly lowered after 30 min of DBP treatment,indicating that DBP owned the inhibitory effect on glucose transportation.Furthermore,DBP also owned protective effects on the barrier function of intestinal epithelial cells.展开更多
Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptide...Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptides from yak bone collagen.The prepared yak bone collagen peptides(YBCPs)was separated,purified and identified through ultrafiltration,reverse-phase high-performance liquid chromatography(RP-HPLC),and nano-liquid chromatography-tandem mass spectrometry(nano LC-MS/MS)successively.Finally,a total of 115 peptides were identified.After that,12 peptides were screened out based on the predicted biological activity score.Molecular docking results indicated that 6 peptides(GPAGPSGPAGK,GPAGPSGPAGKDGR,GPSGPQGIR,GPAGPQGPR,GEAGPAGPAGPAGPR,and GEGGPQGPR)could successfully interact with the key factors in the nuclear factor kappa-B(NF-κB)signaling pathway and nitric oxide(NO)production due to form various bonds such as salt bridge,conventional hydrogen bond,and carbon-hydrogen bond.The anti-inflammatory effects of these 6 peptides were further verified by in vitro cell test.Results indicated that the 6 peptides might play an anti-inflammatory role by regulating different proteins in the NF-κB signaling pathway and NO production.This study would further promote the application of yak bone collagen in health food.展开更多
基金supported by the China Postdoctoral Science Foundation(2021M701462)the National Natural Science Foundation of China(32072254)+2 种基金the Postdoctoral Research Funding Program of Jiangsu Province(2021K097A)the Fundamental Research Funds for the Central Universities(JUSRP121106)the“Qing Lan Project”of Jiangsu Province。
文摘The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are barely understood.This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells.The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL,which did not cause disorder in the normal cell metabolism and absorption activity.The Caco-2 cell monolayer model could absorb DBP by passive and active transport,and the absorptive pattern was dosedependent when the concentration was more than 0.25 mg/mL.During DBP absorption,an increase in m RNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP.But the glucose transport amounts were significantly lowered after 30 min of DBP treatment,indicating that DBP owned the inhibitory effect on glucose transportation.Furthermore,DBP also owned protective effects on the barrier function of intestinal epithelial cells.
基金This work was supported by Postdoctoral Science Foundation of Jiangsu Province(2021K269B)the National Key Research&Developmental Program of China(2021YFC2100300)+1 种基金China Agriculture Research System of Finance and Agriculture Ministry(No.CARS-11-HNSHY)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630052021012,1630052020023,1630052020024).
文摘Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptides from yak bone collagen.The prepared yak bone collagen peptides(YBCPs)was separated,purified and identified through ultrafiltration,reverse-phase high-performance liquid chromatography(RP-HPLC),and nano-liquid chromatography-tandem mass spectrometry(nano LC-MS/MS)successively.Finally,a total of 115 peptides were identified.After that,12 peptides were screened out based on the predicted biological activity score.Molecular docking results indicated that 6 peptides(GPAGPSGPAGK,GPAGPSGPAGKDGR,GPSGPQGIR,GPAGPQGPR,GEAGPAGPAGPAGPR,and GEGGPQGPR)could successfully interact with the key factors in the nuclear factor kappa-B(NF-κB)signaling pathway and nitric oxide(NO)production due to form various bonds such as salt bridge,conventional hydrogen bond,and carbon-hydrogen bond.The anti-inflammatory effects of these 6 peptides were further verified by in vitro cell test.Results indicated that the 6 peptides might play an anti-inflammatory role by regulating different proteins in the NF-κB signaling pathway and NO production.This study would further promote the application of yak bone collagen in health food.
