From Jan</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> 2013 to Nov</span><span style="font-family:Verdana;">.<...From Jan</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> 2013 to Nov</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> 2018, we carried out the investigation on vegetation studies in the ecological monitoring programs, Shenzhen</span><span style="font-family:Verdana;"> and</span><span style="font-family:""><span style="font-family:Verdana;"> totally researched 170 plant communities of mountain areas etc., </span><span style="font-family:Verdana;">few areas of street regions and parks etc., for communities’ structural characteristics and plant diversity. We comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level and the main affected factors. We also researched and analyzed these communities’ structural characteristics and pant diversity, </span><span style="font-family:Verdana;">comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level, the main affected factors;researched and analyzed the relationship between plant community structure characteristics with the absorbing ability to PM</span><sub><span style="font-family:Verdana;">2.5</span></sub><span style="font-family:Verdana;"> pollutants. The result showed that the structure and plant diversity of natural </span><span style="font-family:Verdana;">forest </span></span><span style="font-family:Verdana;">were </span><span style="font-family:Verdana;">obviously better than</span><span style="font-family:Verdana;"> artificial disturbed forest or artificial forest, the semi-natural forest w</span><span style="font-family:Verdana;">as</span><span style="font-family:Verdana;"> the middle level of the forests, In the indices of height, DBH, coverage and crow width etc. of tree layer, natural forests and semi-natural forests were obvious higher than that of artificial disturbed forest or artificial forest;in some forests of artificial disturbed forest or artificial forest, though their richness indices of family and genus were a slightly higher, but this contribution was majorly due to herb layer plants, and some shrub layer plants. Analyz</span><span style="font-family:Verdana;">ing</span><span style="font-family:Verdana;"> three kinds of communities, the number of tree layer species of natural forest was</span><span style="font-family:Verdana;"> the </span><span style="font-family:""><span style="font-family:Verdana;">largest, and their every </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity index value was </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">highest;these characteristics values of semi-natural forest were second, artificial disturbed forests or artificial forests were </span><span style="font-family:Verdana;">the </span><span style="font-family:""><span style="font-family:Verdana;">lowest;in shrub layer, and herb layer, few </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity indices of artificial disturbed forest or artificial forest were </span><span style="font-family:Verdana;">a slightly higher than that of some natural forests, but the integral values of the diversity indices of natural forest and semi-natural forest were obvious</span></span><span style="font-family:Verdana;">ly</span><span style="font-family:Verdana;"> higher than the former. The research showed that in the artificial disturbed forest or artificial forest, because there have some little scale clearing area</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;">, so some pioneer plant species could enter the community and formed a temporarily </span><span style="font-family:Verdana;">increase of species diversity, however</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> these species major </span><span style="font-family:Verdana;">were</span><span style="font-family:Verdana;"> some shrub and herb plants;but tree layer possessed obvious</span><span style="font-family:Verdana;">ly</span><span style="font-family:Verdana;"> more, even surpass</span><span style="font-family:Verdana;">ed</span><span style="font-family:Verdana;"> several ten times biomass than shrub and herb layers</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> Therefore</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> the respect</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> of maintain ecosystem stability and other</span><span style="font-family:Verdana;"> ecological efficient have </span><span style="font-family:Verdana;">major and dominated position and so on;and then this community can </span></span><span style="font-family:Verdana;">be </span><span style="font-family:Verdana;">natural recovery and succession, those forementioned new entire plants shall withdraw from the community and are replaced by the original plants of the community;but this process has already caused the ecological efficient loss and ecosystem unstable. Above research results are better evidence and theory reference to the argument problems on that </span><span style="font-family:Verdana;">are</span><span style="font-family:""><span style="font-family:Verdana;"> natural forest and natural restoration forest biodiversity higher? or is artificial forest biodiversity higher? and or is more artificial disturbed forest higher?</span><span style="font-family:Verdana;"> Our researches showed that used random investigation method set quadrats, and combined typical investigation method, the structural indices in the all layer of community and all diversity indices of tree, shrub and herb layers and the integral values </span></span><span style="font-family:Verdana;">were</span><span style="font-family:""><span style="font-family:Verdana;"> similar, same or usually these indices in the community which set 600 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, 800 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total quatrat area were higher (</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity) than that of more than 3000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, 4000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> or 7000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, even more than 17,000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total qua</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;">rat area of communities. These lot</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> of researches further proved that according to minimum area method for vegetation survey, in subtropical region, using random investigation method combined with typical investigation set 400 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> or 400 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> - 500 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total qua</span></span><span style="font-family:Verdana;">d</span><span style="font-family:""><span展开更多
Acute lung injury(ALI)has been a kind of acute and severe disease that is mainly charac-terized by systemic uncontrolled inflammatory response to the production of huge amounts of reactive oxygen species(ROS)in the lu...Acute lung injury(ALI)has been a kind of acute and severe disease that is mainly charac-terized by systemic uncontrolled inflammatory response to the production of huge amounts of reactive oxygen species(ROS)in the lung tissue.Given the critical role of ROS in ALI,a Fe_(3)O_(4) loaded bovine serum albumin(BSA)nanocluster(BF)was developed to act as a nanomedicine for the treatment of ALI.Combining with NIR irradiation,it exhibited excellent ROS scavenging capacity.Significantly,it also dis-played the excellent antioxidant and anti-inflammatory functions for lipopolysaccharides(LPS)induced macrophages(RAW264.7),and Sprague Dawley rats via lowering intracellular ROS levels,reducing inflammatory factors expression levels,inducing macrophage M2 polarization,inhibiting NF-κB signaling pathway,increasing CD4^(+)/CD8^(+)T cell ratios,as well as upregulating HSP70 and CD31 expression levels to reprogram redox homeostasis,reduce systemic inflammation,activate immunoregu-lation,and accelerate lung tissue repair,finally achieving the synergistic enhancement of ALI immuno-therapy.It finally provides an effective therapeutic strategy of BF+NIR for the management of inflammation related diseases.展开更多
Diabetic infectious wound is usually in the environment of high glucose levels and immune disorder.It always results in high reactive oxygen species(ROS)levels,serious immune dysfunction,and extreme susceptibility to ...Diabetic infectious wound is usually in the environment of high glucose levels and immune disorder.It always results in high reactive oxygen species(ROS)levels,serious immune dysfunction,and extreme susceptibility to infection,delaying wound healing.Herein,we prepared an immunomodulatory polyphenol metal organic framework nanozyme(CE)combined with near infrared(NIR)irradiation for diabetic infectious wound healing.This nanozyme was formed by the self-assembly of Cu ions and ellagic acid.In vitro experiments confirmed that CE+NIR could efficiently lower the inflammatory factors(IL-6(50.72%),IL-1β(61.44%)and iNOS(57.33%))expression,and upregulate anti-inflammatory factor(IL-10(111.44%))expression of lipopolysaccharide induced RAW264.7,decreasing the ROS levels,promoting cellular migration(527.91%),and accelerating angiogenesis(91.81%)of high glucose treated L929,together with inhibiting the bacterial growth of E.coli(92.21%)and MRSA(95.89%).Transcriptome sequencing results and their related validation experiments demonstrated that CE+NIR achieved antioxidant and anti-inflammation through ferroptosis inhibition.And transcriptomics and metabolomics analysis proved the efficient antibacterial activity of CE+NIR through NIR amplified cuproptosis.Markedly,it also confirmed that the strategy of CE+NIR was helpful to immunoregu-lation activation via inducing macrophage M2 directional polarization,and immune activating T cells number,thereby restoring immune homeostasis,and accelerating tissue repair.It might offer an efficient strategy of other infectious diseases’immunotherapy with high efficacy and biosafety.展开更多
文摘From Jan</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> 2013 to Nov</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> 2018, we carried out the investigation on vegetation studies in the ecological monitoring programs, Shenzhen</span><span style="font-family:Verdana;"> and</span><span style="font-family:""><span style="font-family:Verdana;"> totally researched 170 plant communities of mountain areas etc., </span><span style="font-family:Verdana;">few areas of street regions and parks etc., for communities’ structural characteristics and plant diversity. We comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level and the main affected factors. We also researched and analyzed these communities’ structural characteristics and pant diversity, </span><span style="font-family:Verdana;">comparatively analyzed natural forests, semi-natural forest and artificial forest composition and structural characters and family, genus and species diversity level, the main affected factors;researched and analyzed the relationship between plant community structure characteristics with the absorbing ability to PM</span><sub><span style="font-family:Verdana;">2.5</span></sub><span style="font-family:Verdana;"> pollutants. The result showed that the structure and plant diversity of natural </span><span style="font-family:Verdana;">forest </span></span><span style="font-family:Verdana;">were </span><span style="font-family:Verdana;">obviously better than</span><span style="font-family:Verdana;"> artificial disturbed forest or artificial forest, the semi-natural forest w</span><span style="font-family:Verdana;">as</span><span style="font-family:Verdana;"> the middle level of the forests, In the indices of height, DBH, coverage and crow width etc. of tree layer, natural forests and semi-natural forests were obvious higher than that of artificial disturbed forest or artificial forest;in some forests of artificial disturbed forest or artificial forest, though their richness indices of family and genus were a slightly higher, but this contribution was majorly due to herb layer plants, and some shrub layer plants. Analyz</span><span style="font-family:Verdana;">ing</span><span style="font-family:Verdana;"> three kinds of communities, the number of tree layer species of natural forest was</span><span style="font-family:Verdana;"> the </span><span style="font-family:""><span style="font-family:Verdana;">largest, and their every </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity index value was </span></span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">highest;these characteristics values of semi-natural forest were second, artificial disturbed forests or artificial forests were </span><span style="font-family:Verdana;">the </span><span style="font-family:""><span style="font-family:Verdana;">lowest;in shrub layer, and herb layer, few </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity indices of artificial disturbed forest or artificial forest were </span><span style="font-family:Verdana;">a slightly higher than that of some natural forests, but the integral values of the diversity indices of natural forest and semi-natural forest were obvious</span></span><span style="font-family:Verdana;">ly</span><span style="font-family:Verdana;"> higher than the former. The research showed that in the artificial disturbed forest or artificial forest, because there have some little scale clearing area</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;">, so some pioneer plant species could enter the community and formed a temporarily </span><span style="font-family:Verdana;">increase of species diversity, however</span></span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> these species major </span><span style="font-family:Verdana;">were</span><span style="font-family:Verdana;"> some shrub and herb plants;but tree layer possessed obvious</span><span style="font-family:Verdana;">ly</span><span style="font-family:Verdana;"> more, even surpass</span><span style="font-family:Verdana;">ed</span><span style="font-family:Verdana;"> several ten times biomass than shrub and herb layers</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> Therefore</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> the respect</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> of maintain ecosystem stability and other</span><span style="font-family:Verdana;"> ecological efficient have </span><span style="font-family:Verdana;">major and dominated position and so on;and then this community can </span></span><span style="font-family:Verdana;">be </span><span style="font-family:Verdana;">natural recovery and succession, those forementioned new entire plants shall withdraw from the community and are replaced by the original plants of the community;but this process has already caused the ecological efficient loss and ecosystem unstable. Above research results are better evidence and theory reference to the argument problems on that </span><span style="font-family:Verdana;">are</span><span style="font-family:""><span style="font-family:Verdana;"> natural forest and natural restoration forest biodiversity higher? or is artificial forest biodiversity higher? and or is more artificial disturbed forest higher?</span><span style="font-family:Verdana;"> Our researches showed that used random investigation method set quadrats, and combined typical investigation method, the structural indices in the all layer of community and all diversity indices of tree, shrub and herb layers and the integral values </span></span><span style="font-family:Verdana;">were</span><span style="font-family:""><span style="font-family:Verdana;"> similar, same or usually these indices in the community which set 600 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, 800 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total quatrat area were higher (</span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-diversity) than that of more than 3000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, 4000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> or 7000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">, even more than 17,000 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total qua</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;">rat area of communities. These lot</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> of researches further proved that according to minimum area method for vegetation survey, in subtropical region, using random investigation method combined with typical investigation set 400 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> or 400 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> - 500 m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> total qua</span></span><span style="font-family:Verdana;">d</span><span style="font-family:""><span
基金supported by the Joint Project on Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation(Nos.2024GXNSFAA010089 and 2025GXNSFBA069275,China)the National Natural Science Foundation of China(No.82160902,China)+2 种基金the China Post-doctoral Science Foundation(No.2024MD753921,China)the Key Laboratory of Micro-Nanoscale Bioanalysis and Drug Screening,Education Department of Guangxi Zhuang Autono-mous Region(GXYWSX202404,China)the First-class Disci-pline Innovation-driven Talent Program of Guangxi Medical University,and the Major Talent Project of Guangxi Autonomous Region.
文摘Acute lung injury(ALI)has been a kind of acute and severe disease that is mainly charac-terized by systemic uncontrolled inflammatory response to the production of huge amounts of reactive oxygen species(ROS)in the lung tissue.Given the critical role of ROS in ALI,a Fe_(3)O_(4) loaded bovine serum albumin(BSA)nanocluster(BF)was developed to act as a nanomedicine for the treatment of ALI.Combining with NIR irradiation,it exhibited excellent ROS scavenging capacity.Significantly,it also dis-played the excellent antioxidant and anti-inflammatory functions for lipopolysaccharides(LPS)induced macrophages(RAW264.7),and Sprague Dawley rats via lowering intracellular ROS levels,reducing inflammatory factors expression levels,inducing macrophage M2 polarization,inhibiting NF-κB signaling pathway,increasing CD4^(+)/CD8^(+)T cell ratios,as well as upregulating HSP70 and CD31 expression levels to reprogram redox homeostasis,reduce systemic inflammation,activate immunoregu-lation,and accelerate lung tissue repair,finally achieving the synergistic enhancement of ALI immuno-therapy.It finally provides an effective therapeutic strategy of BF+NIR for the management of inflammation related diseases.
基金financially supported by the Key Research&Development Program of Guangxi(Grant No.GuiKeAB22080088)the National Natural Science Foundation of China(Grant No.82360372)+2 种基金the Key Project of Hunan Provincial Administration of Traditional Chinese Medicine(Grant No.C2024004)the First-class Discipline Innovation-driven Talent Program of Guangxi Medical Universitythe Major Talent Project of Guangxi Autonomous Region.
文摘Diabetic infectious wound is usually in the environment of high glucose levels and immune disorder.It always results in high reactive oxygen species(ROS)levels,serious immune dysfunction,and extreme susceptibility to infection,delaying wound healing.Herein,we prepared an immunomodulatory polyphenol metal organic framework nanozyme(CE)combined with near infrared(NIR)irradiation for diabetic infectious wound healing.This nanozyme was formed by the self-assembly of Cu ions and ellagic acid.In vitro experiments confirmed that CE+NIR could efficiently lower the inflammatory factors(IL-6(50.72%),IL-1β(61.44%)and iNOS(57.33%))expression,and upregulate anti-inflammatory factor(IL-10(111.44%))expression of lipopolysaccharide induced RAW264.7,decreasing the ROS levels,promoting cellular migration(527.91%),and accelerating angiogenesis(91.81%)of high glucose treated L929,together with inhibiting the bacterial growth of E.coli(92.21%)and MRSA(95.89%).Transcriptome sequencing results and their related validation experiments demonstrated that CE+NIR achieved antioxidant and anti-inflammation through ferroptosis inhibition.And transcriptomics and metabolomics analysis proved the efficient antibacterial activity of CE+NIR through NIR amplified cuproptosis.Markedly,it also confirmed that the strategy of CE+NIR was helpful to immunoregu-lation activation via inducing macrophage M2 directional polarization,and immune activating T cells number,thereby restoring immune homeostasis,and accelerating tissue repair.It might offer an efficient strategy of other infectious diseases’immunotherapy with high efficacy and biosafety.
