Breeding hybrids with nuclear malesterile lines is an important method for the cross-breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper. Yet, there ar...Breeding hybrids with nuclear malesterile lines is an important method for the cross-breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper. Yet, there are approximately 20 pepper nuclear malesterility lines in the world. Using the self-developed testing material, sweet pepper nuclear malesterile dual-purpose line AB91, the genome-wide resequencing technique was applied to find that the mutation site causing the abortion of sweet pepper nuclear malesterility AB91 is on chromosome #5. The mutation gene Capana05g000747 was filtered out and validated by the flight mass spectrometry genotyping and quantitative realtime PCR method and determined to be the gene causing the abortion of sweet pepper nuclear male sterility AB91. The gene Capana05g000747 mutation site is a non-synonymous mutation site located at the 6th exon, the base C mutated into A, and the amino acid changed from alanine to serine. The three-dimensional protein structure of fertile and sterile plant Capana05g000747 was predicted. The results showed that the three-dimensional structure of the two proteins differed significantly. Sequence alignment analysis showed that the gene Capana05g000747 has a similar function to gene At2g02148. The gene At2g02148 contains a pentatricopeptide repeat protein which has important physiological functions in the gene expression process of organelles and is closely related to the performance of malesterility genes. Therefore, Capana05g000747 was selected as an important candidate gene for sweet pepper nuclear male sterile testing material AB91.展开更多
Coal spontaneous combustion is a great threat to mine safety,and gas is the key index to describe coal spontaneous combustion.Taking the coal samples of different kinds of coal as research object,the temperature progr...Coal spontaneous combustion is a great threat to mine safety,and gas is the key index to describe coal spontaneous combustion.Taking the coal samples of different kinds of coal as research object,the temperature programmed oxidation experiment was carried out,and the gases produced by coal samples at different temperatures were collected and analyzed by gas chromatography.This research studied the variation characteristics of gas species and gas concentrations in different coal samples during heating oxidation.The experimental results show that different coal samples produce different kinds of gases in the process of heating and oxidation.The order of gas production is CO,C2H6,C2H4,C3H8,and the relationship between gas production and temperature is approximately exponential.With the increase of coal metamorphic degree,the turning point temperature of sharp rise in coal sample gas production rate become higher,the oxidation ability of coal sample decreases,and the quantity of gas production decreases during the same time period.展开更多
In view of the lack of wheat genetic resources with high amylose and high resistant starch contents in the present world,the grain starch components and SGP-1 (SGP-A1,SGP-B1 and SGP-D1) protein composition of 43 black...In view of the lack of wheat genetic resources with high amylose and high resistant starch contents in the present world,the grain starch components and SGP-1 (SGP-A1,SGP-B1 and SGP-D1) protein composition of 43 black-kernel wheat genetic resources such as Jizi 439 were identified by SDS-PAGE electrophoresis detection.The results showed that 11 materials were lack of SGP-A1,and no materials had SGP-B1 and SGP-D1 deletion.Seven materials were identified to have an amylose content more than 30% of the total starch.A total of 12 materials were selected for the determination of resistant starch content,and five materials were found to have a high resistant starch content.The above results lay a foundation for wheat breeding for high resistant starch content.展开更多
文摘Breeding hybrids with nuclear malesterile lines is an important method for the cross-breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper. Yet, there are approximately 20 pepper nuclear malesterility lines in the world. Using the self-developed testing material, sweet pepper nuclear malesterile dual-purpose line AB91, the genome-wide resequencing technique was applied to find that the mutation site causing the abortion of sweet pepper nuclear malesterility AB91 is on chromosome #5. The mutation gene Capana05g000747 was filtered out and validated by the flight mass spectrometry genotyping and quantitative realtime PCR method and determined to be the gene causing the abortion of sweet pepper nuclear male sterility AB91. The gene Capana05g000747 mutation site is a non-synonymous mutation site located at the 6th exon, the base C mutated into A, and the amino acid changed from alanine to serine. The three-dimensional protein structure of fertile and sterile plant Capana05g000747 was predicted. The results showed that the three-dimensional structure of the two proteins differed significantly. Sequence alignment analysis showed that the gene Capana05g000747 has a similar function to gene At2g02148. The gene At2g02148 contains a pentatricopeptide repeat protein which has important physiological functions in the gene expression process of organelles and is closely related to the performance of malesterility genes. Therefore, Capana05g000747 was selected as an important candidate gene for sweet pepper nuclear male sterile testing material AB91.
基金National Natural Science Foundation of China(No.51474086)Hebei Province Natural Science Foundation(No.E2014209138)the North China University of Science and Technology Fostering Fund Project(No.GP201511)for the financial supports
文摘Coal spontaneous combustion is a great threat to mine safety,and gas is the key index to describe coal spontaneous combustion.Taking the coal samples of different kinds of coal as research object,the temperature programmed oxidation experiment was carried out,and the gases produced by coal samples at different temperatures were collected and analyzed by gas chromatography.This research studied the variation characteristics of gas species and gas concentrations in different coal samples during heating oxidation.The experimental results show that different coal samples produce different kinds of gases in the process of heating and oxidation.The order of gas production is CO,C2H6,C2H4,C3H8,and the relationship between gas production and temperature is approximately exponential.With the increase of coal metamorphic degree,the turning point temperature of sharp rise in coal sample gas production rate become higher,the oxidation ability of coal sample decreases,and the quantity of gas production decreases during the same time period.
基金Supported by National Science and Technology Support Program(2013BAD01B02-11)National Natural Science Foundation of China(31201209)+1 种基金National Key Research and Development Project(2016YFD0100102-5)Science and Technology Support Program of Hebei Province(16226320D)
文摘In view of the lack of wheat genetic resources with high amylose and high resistant starch contents in the present world,the grain starch components and SGP-1 (SGP-A1,SGP-B1 and SGP-D1) protein composition of 43 black-kernel wheat genetic resources such as Jizi 439 were identified by SDS-PAGE electrophoresis detection.The results showed that 11 materials were lack of SGP-A1,and no materials had SGP-B1 and SGP-D1 deletion.Seven materials were identified to have an amylose content more than 30% of the total starch.A total of 12 materials were selected for the determination of resistant starch content,and five materials were found to have a high resistant starch content.The above results lay a foundation for wheat breeding for high resistant starch content.
