Traditional submarine tunnels with drainage systems are highly susceptible to water leakage,which is challenging to locate and manage,leading to high maintenance costs.To address this problem,a new controlled drainage...Traditional submarine tunnels with drainage systems are highly susceptible to water leakage,which is challenging to locate and manage,leading to high maintenance costs.To address this problem,a new controlled drainage system for submarine tunnels was proposed in this study.The system utilizes a double-adhesive waterproof membrane instead of traditional waterproof sheets,which not only reduces the likelihood of leakage but also makes subsequent leak detection and repair easier.In addition,replacing blind pipes with drainage sheets significantly improves the clogging resistance of the drainage system.The influence of grouting circles and drainage sheets on the water inflow and the external water pressure on the lining was then analyzed using numerical calculation methods.Finally,the design process of the new controlled drainage system was proposed.The research suggests that the new system allows for a multi-stage control method using grouting circles and drainage sheets,providing greater design flexibility.In the primary control stage,grouting circles effectively reduce the tunnel water inflow and the external water pressure on the lining,with the permeability coefficient playing a crucial role.In the secondary control stage,the spacing and width of the drainage sheets can regulate the water inflow and pressure.Unlike grouting circles,drainage sheets decrease water pressure while inevitably increasing water inflow,and vice versa.These findings can serve as a valuable reference for the design of waterproof and drainage systems in submarine tunnels.展开更多
Quantum singular value thresholding(QSVT) algorithm,as a core module of many mathematical models,seeks the singular values of a sparse and low rank matrix exceeding a threshold and their associated singular vectors.Th...Quantum singular value thresholding(QSVT) algorithm,as a core module of many mathematical models,seeks the singular values of a sparse and low rank matrix exceeding a threshold and their associated singular vectors.The existing all-qubit QSVT algorithm demands lots of ancillary qubits,remaining a huge challenge for realization on nearterm intermediate-scale quantum computers.In this paper,we propose a hybrid QSVT(HQSVT) algorithm utilizing both discrete variables(DVs) and continuous variables(CVs).In our algorithm,raw data vectors are encoded into a qubit system and the following data processing is fulfilled by hybrid quantum operations.Our algorithm requires O [log(MN)] qubits with0(1) qumodes and totally performs 0(1) operations,which significantly reduces the space and runtime consumption.展开更多
Double-bonded spray membrane waterproofing materials have excellent waterproofing performance and can improve the load-bearing capacity of tunnel linings,leading to an increasing global application.However,due to the ...Double-bonded spray membrane waterproofing materials have excellent waterproofing performance and can improve the load-bearing capacity of tunnel linings,leading to an increasing global application.However,due to the double-bonded capability of spray membrane materials,traditional interlayer drainage methods cannot be applied.This limitation makes it difficult to use them in drainage-type tunnels,significantly restricting their range of applications.In this regard,a novel tunnel waterproof-drainage system based on double-bonded spray membrane materials was proposed in this paper.The proposed drainage system primarily comprises upper drainage sheets and bottom drainage blind pipes,both located in the tunnel circumferential direction,as well as longitudinal drainage pipes within the tunnel.Subsequently,numerical calculation methods are employed to analyze the seepage characteristics of this system,revealing the water pressure distribution around the tunnel.The results indicate that in the novel waterproof-drainage system,the water pressure in the secondary lining exhibits a“mushroom-shaped”distribution in the circumferential direction,while the water pressure in the longitudinal direction exhibits a“wave-like”distribution.Furthermore,comparative results with other waterproof-drainage systems indicate that under typical working conditions with a water head of 160 m and a rock permeability coefficient of 10^(−6)m/s,the maximum water pressure in the secondary lining of the novel waterproof-drainage system is 0.6 MPa.This represents a significant reduction compared to fully encapsulated waterproofing and traditional drainage systems,which respectively reduce the water pressure by 65%and 30%.The applicability analysis of the double-bonded waterproofing and drainage system reveals that it can reduce at least 40%of the static water pressure in any groundwater environments.The novel drainage system provides a valuable reference for the application of double-bonded spray membrane waterproofing materials in drainage-type tunnels.展开更多
Emerging evidence has demonstrated that stromal cell-derived factor 1(SDF-1)and its cognate receptor CXCR4 have critical roles in tumorigenesis,angiogenesis and metastasis.In this study,we demonstrated the significant...Emerging evidence has demonstrated that stromal cell-derived factor 1(SDF-1)and its cognate receptor CXCR4 have critical roles in tumorigenesis,angiogenesis and metastasis.In this study,we demonstrated the significant inhibitory effects of a novel chemically synthetic peptide(E5)on the CXCR4/CXCL12 axis in breast cancer both in vitro and in vivo.E5 was capable of specifically binding to the murine breast cancer cell line 4T1,remarkably inhibiting CXCL12-or stromal cell(MS-5)-induced migration,and adhesion and sensitizing 4T1 cells to multiple chemotherapeutic drugs.Furthermore,E5 combined with either paclitaxel or cyclophosphamide significantly inhibited tumor growth in a breast cancer model.Mechanistic studies implied that E5 can inhibit the expression of CXCR4 to block the CXCL12-mediated recruitment of endothelial progenitor cells and repress CXCR4 downstream of the Akt and Erk signaling pathway,which are involved in tumor angiogenesis and progression.Further pharmacokinetic evaluation suggested that E5 has an acceptable stability,with a half-life of 10 h in healthy mice.In conclusion,E5 demonstrates a promising anti-tumor effect and could be a potential chemotherapeutic sensitizer to improve current clinical breast cancer therapies.展开更多
Objectives:Fractionated radiotherapy represents a standardized and widely adopted treatment modality for cancermanagement,with approximately 40% of non-small cell lung cancer(NSCLC)patients receiving it.However,repeat...Objectives:Fractionated radiotherapy represents a standardized and widely adopted treatment modality for cancermanagement,with approximately 40% of non-small cell lung cancer(NSCLC)patients receiving it.However,repeated irradiationmay induce radioresistance in cancer cells,reducing treatment effectiveness and raising recurrence risk.The long noncoding RNA CRYBG3(lncRNA CRYBG3),which is upregulated in lung cancer cells after Xray irradiation,contributes to the radioresistance of NSCLC cells by promoting wild-type p53 protein degradation.This study aims to elucidate the mechanism of fractionated irradiation-induced radioresistance,in which lncRNA CRYBG3 regulates radiation-induced mitochondrial damage and reactive oxygen species(ROS)generation through the p53 downstream signaling pathway.Methods:To investigate the critical roles of lncRNA CRYBG3 in mediating radioresistance induced by fractionated irradiation in NSCLC,we established radioresistant NSCLC cells by irradiating the A549 and H460 cell lines with 60 Gy of X-rays in 12 fractions,and named the radioresistant cells A549R and H460R,respectively.Lentiviral vectors were used to deliver short hairpin RNA(shRNA)into cells to knock down lncRNA CRYBG3,thereby investigating its contribution to adaptive radioresistance in A549R and H460R cells.All cells were irradiated with 4 Gy of X-rays,and subsequent analyses were conducted to evaluate mitochondrial damage,ROS generation,apoptosis,and the expression of oxidative stress-related proteins.Results:Increased expression levels of lncRNA CRYBG3 were positively associated with the acquisition of radioresistance in NSCLC cells.Additionally,suppressing lncRNA CRYBG3 increased mitochondrial damage and promoted radiation-induced apoptosis in radioresistant NSCLC cells.Mechanistically,the downregulation of lncRNA CRYBG3 led to increased p53 levels,resulting in decreased expression of heat shock factor 1(HSF1)and tumor necrosis factor receptor-associated protein 1(TRAP1),as well as enhanced radiation-induced mitochondrial oxidative damage and apoptosis.Conclusion:The results indicate that lncRNA CRYBG3 plays a regulatory role in adaptive radioresistance in NSCLC cells through the p53/HSF1/TRAP1 axis.Therefore,targeting lncRNA CRYBG3 could potentially improve the efficacy of fractionated radiotherapy in NSCLC.展开更多
基金supported by the Fundamental Research Funds for the Central Universities of Central South University(No.2023ZZTS0183)the Fundamental Research Funds for the Central Universities(No.502802002).
文摘Traditional submarine tunnels with drainage systems are highly susceptible to water leakage,which is challenging to locate and manage,leading to high maintenance costs.To address this problem,a new controlled drainage system for submarine tunnels was proposed in this study.The system utilizes a double-adhesive waterproof membrane instead of traditional waterproof sheets,which not only reduces the likelihood of leakage but also makes subsequent leak detection and repair easier.In addition,replacing blind pipes with drainage sheets significantly improves the clogging resistance of the drainage system.The influence of grouting circles and drainage sheets on the water inflow and the external water pressure on the lining was then analyzed using numerical calculation methods.Finally,the design process of the new controlled drainage system was proposed.The research suggests that the new system allows for a multi-stage control method using grouting circles and drainage sheets,providing greater design flexibility.In the primary control stage,grouting circles effectively reduce the tunnel water inflow and the external water pressure on the lining,with the permeability coefficient playing a crucial role.In the secondary control stage,the spacing and width of the drainage sheets can regulate the water inflow and pressure.Unlike grouting circles,drainage sheets decrease water pressure while inevitably increasing water inflow,and vice versa.These findings can serve as a valuable reference for the design of waterproof and drainage systems in submarine tunnels.
基金Project supported by the Key Research and Development Program of Guangdong Province,China(Grant No.2018B030326001)the National Natural Science Foundation of China(Grant Nos.61521001,12074179,and 11890704)。
文摘Quantum singular value thresholding(QSVT) algorithm,as a core module of many mathematical models,seeks the singular values of a sparse and low rank matrix exceeding a threshold and their associated singular vectors.The existing all-qubit QSVT algorithm demands lots of ancillary qubits,remaining a huge challenge for realization on nearterm intermediate-scale quantum computers.In this paper,we propose a hybrid QSVT(HQSVT) algorithm utilizing both discrete variables(DVs) and continuous variables(CVs).In our algorithm,raw data vectors are encoded into a qubit system and the following data processing is fulfilled by hybrid quantum operations.Our algorithm requires O [log(MN)] qubits with0(1) qumodes and totally performs 0(1) operations,which significantly reduces the space and runtime consumption.
基金supported by the Fundamental Research Funds for the Central Universities of Central South University(No.2023ZZTS0183)the Fundamental Research Funds for the Central Universities(No.502802002).
文摘Double-bonded spray membrane waterproofing materials have excellent waterproofing performance and can improve the load-bearing capacity of tunnel linings,leading to an increasing global application.However,due to the double-bonded capability of spray membrane materials,traditional interlayer drainage methods cannot be applied.This limitation makes it difficult to use them in drainage-type tunnels,significantly restricting their range of applications.In this regard,a novel tunnel waterproof-drainage system based on double-bonded spray membrane materials was proposed in this paper.The proposed drainage system primarily comprises upper drainage sheets and bottom drainage blind pipes,both located in the tunnel circumferential direction,as well as longitudinal drainage pipes within the tunnel.Subsequently,numerical calculation methods are employed to analyze the seepage characteristics of this system,revealing the water pressure distribution around the tunnel.The results indicate that in the novel waterproof-drainage system,the water pressure in the secondary lining exhibits a“mushroom-shaped”distribution in the circumferential direction,while the water pressure in the longitudinal direction exhibits a“wave-like”distribution.Furthermore,comparative results with other waterproof-drainage systems indicate that under typical working conditions with a water head of 160 m and a rock permeability coefficient of 10^(−6)m/s,the maximum water pressure in the secondary lining of the novel waterproof-drainage system is 0.6 MPa.This represents a significant reduction compared to fully encapsulated waterproofing and traditional drainage systems,which respectively reduce the water pressure by 65%and 30%.The applicability analysis of the double-bonded waterproofing and drainage system reveals that it can reduce at least 40%of the static water pressure in any groundwater environments.The novel drainage system provides a valuable reference for the application of double-bonded spray membrane waterproofing materials in drainage-type tunnels.
基金This work was supported by the CAMS Innovation Fund for Medical Sciences(CIFMS,2016-I2M-3-004)the Key Project of the Major Research Plan from the National Natural Science Foundation of China(91127043).
文摘Emerging evidence has demonstrated that stromal cell-derived factor 1(SDF-1)and its cognate receptor CXCR4 have critical roles in tumorigenesis,angiogenesis and metastasis.In this study,we demonstrated the significant inhibitory effects of a novel chemically synthetic peptide(E5)on the CXCR4/CXCL12 axis in breast cancer both in vitro and in vivo.E5 was capable of specifically binding to the murine breast cancer cell line 4T1,remarkably inhibiting CXCL12-or stromal cell(MS-5)-induced migration,and adhesion and sensitizing 4T1 cells to multiple chemotherapeutic drugs.Furthermore,E5 combined with either paclitaxel or cyclophosphamide significantly inhibited tumor growth in a breast cancer model.Mechanistic studies implied that E5 can inhibit the expression of CXCR4 to block the CXCL12-mediated recruitment of endothelial progenitor cells and repress CXCR4 downstream of the Akt and Erk signaling pathway,which are involved in tumor angiogenesis and progression.Further pharmacokinetic evaluation suggested that E5 has an acceptable stability,with a half-life of 10 h in healthy mice.In conclusion,E5 demonstrates a promising anti-tumor effect and could be a potential chemotherapeutic sensitizer to improve current clinical breast cancer therapies.
基金supported by the National Natural Science Foundation of China(No.12205215)Suzhou Fundamental Research Project(No.SJC2023001)+2 种基金Basic Research Program of Nantong Science and Technology Bureau(No.JC12022103)College Student Innovation Training Program Project of Soochow University(No.202410285101Z)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Objectives:Fractionated radiotherapy represents a standardized and widely adopted treatment modality for cancermanagement,with approximately 40% of non-small cell lung cancer(NSCLC)patients receiving it.However,repeated irradiationmay induce radioresistance in cancer cells,reducing treatment effectiveness and raising recurrence risk.The long noncoding RNA CRYBG3(lncRNA CRYBG3),which is upregulated in lung cancer cells after Xray irradiation,contributes to the radioresistance of NSCLC cells by promoting wild-type p53 protein degradation.This study aims to elucidate the mechanism of fractionated irradiation-induced radioresistance,in which lncRNA CRYBG3 regulates radiation-induced mitochondrial damage and reactive oxygen species(ROS)generation through the p53 downstream signaling pathway.Methods:To investigate the critical roles of lncRNA CRYBG3 in mediating radioresistance induced by fractionated irradiation in NSCLC,we established radioresistant NSCLC cells by irradiating the A549 and H460 cell lines with 60 Gy of X-rays in 12 fractions,and named the radioresistant cells A549R and H460R,respectively.Lentiviral vectors were used to deliver short hairpin RNA(shRNA)into cells to knock down lncRNA CRYBG3,thereby investigating its contribution to adaptive radioresistance in A549R and H460R cells.All cells were irradiated with 4 Gy of X-rays,and subsequent analyses were conducted to evaluate mitochondrial damage,ROS generation,apoptosis,and the expression of oxidative stress-related proteins.Results:Increased expression levels of lncRNA CRYBG3 were positively associated with the acquisition of radioresistance in NSCLC cells.Additionally,suppressing lncRNA CRYBG3 increased mitochondrial damage and promoted radiation-induced apoptosis in radioresistant NSCLC cells.Mechanistically,the downregulation of lncRNA CRYBG3 led to increased p53 levels,resulting in decreased expression of heat shock factor 1(HSF1)and tumor necrosis factor receptor-associated protein 1(TRAP1),as well as enhanced radiation-induced mitochondrial oxidative damage and apoptosis.Conclusion:The results indicate that lncRNA CRYBG3 plays a regulatory role in adaptive radioresistance in NSCLC cells through the p53/HSF1/TRAP1 axis.Therefore,targeting lncRNA CRYBG3 could potentially improve the efficacy of fractionated radiotherapy in NSCLC.
