In this study,a novel co-cultured bacterium associated with Dunaliella salina was isolated using a culturomics approach and was proposed to be named Sinobaca sp.SCU0214.An algal-bacterial co-culture system was constru...In this study,a novel co-cultured bacterium associated with Dunaliella salina was isolated using a culturomics approach and was proposed to be named Sinobaca sp.SCU0214.An algal-bacterial co-culture system was constructed,within which the carotenoid content was increased by 49.66%,reaching 2.61±0.34 mg/L under stable co-culture conditions.The biomass of D.salina reached(2.10±0.34)×10^(6)cells/mL.Total pigment content reached 12.84±1.51 mg/L;chlorophyll-a and chlorophyll-b reached 10.08±1.22 mg/L and 2.75±0.30 mg/L,respectively.The system demonstrated robustness under varying light qualities,with photosynthetic efficiency remaining unaffected.A secondary metabolite,ectoine,produced by SCU0214 and accumulated within the system,was identified.It was demonstrated that ectoine promoted carotenoid production in D.salina and served as a compensatory osmotic regulator.This study presented an effective and innovative algal-bacterial system for enhanced carotenoid production by D.salina,and established ectoine as an interaction-regulating molecule in the co-culture relationship,offering valuable insights for improving carotenoid yields and understanding microbial interactions within such systems.The research findings provide a more efficient production system for the application of carotenoids as high-value additives in food.展开更多
基金supported by National Natural Science Foundation of China(32171473,32271535)Natural Science Foundation of Sichuan Province(2024NSFSC0386,2024NSFSC0035).
文摘In this study,a novel co-cultured bacterium associated with Dunaliella salina was isolated using a culturomics approach and was proposed to be named Sinobaca sp.SCU0214.An algal-bacterial co-culture system was constructed,within which the carotenoid content was increased by 49.66%,reaching 2.61±0.34 mg/L under stable co-culture conditions.The biomass of D.salina reached(2.10±0.34)×10^(6)cells/mL.Total pigment content reached 12.84±1.51 mg/L;chlorophyll-a and chlorophyll-b reached 10.08±1.22 mg/L and 2.75±0.30 mg/L,respectively.The system demonstrated robustness under varying light qualities,with photosynthetic efficiency remaining unaffected.A secondary metabolite,ectoine,produced by SCU0214 and accumulated within the system,was identified.It was demonstrated that ectoine promoted carotenoid production in D.salina and served as a compensatory osmotic regulator.This study presented an effective and innovative algal-bacterial system for enhanced carotenoid production by D.salina,and established ectoine as an interaction-regulating molecule in the co-culture relationship,offering valuable insights for improving carotenoid yields and understanding microbial interactions within such systems.The research findings provide a more efficient production system for the application of carotenoids as high-value additives in food.
