With the progress of urbanization,rural tourism has emerged as a popular leisure activity in China.The crop field with pattern art has been gaining popularity over the years,using strains with colorful leaves to creat...With the progress of urbanization,rural tourism has emerged as a popular leisure activity in China.The crop field with pattern art has been gaining popularity over the years,using strains with colorful leaves to create various impressive designs(Xu 2024).A lot of successful cases in rice fields have not only attracted tourists but also increased the income of farmers(Song et al.2020).Plants with colorful leaves also have gained significant popularity in ornamental agriculture.Therefore,developing plants with colorful leaves has gained significant popularity in ornamental agriculture.展开更多
Peanut seedlings develop from seeds by hypocotyl elongation and differentiation.However,the intricate gene regulatory networks and molecular crosstalk underlying hypocotyl growth in peanuts remain largely unknown.In t...Peanut seedlings develop from seeds by hypocotyl elongation and differentiation.However,the intricate gene regulatory networks and molecular crosstalk underlying hypocotyl growth in peanuts remain largely unknown.In this study,a single-nucleus gene expression landscape in peanut seedlings was initially developed from diverse tissues,including stems,roots,leaves,and hypocotyls.Core transcription factor interaction networks driving developmental trajectories were identified to decipher hypocotyl cell heterogeneity.Jasmonic acid and cytokinin regulate peanut hypocotyl expansion and differentiation based on the number and size of cortex cells and hormone levels between the hypocotyl at 3 and 7 d after germination.We further demonstrated that AhBHLH143 potentially represses hypocotyl elongation by promoting the JA pathway and inhibiting the cytokinin pathway.The single-nucleus transcriptomic atlas of peanut seedlings reveals new insights into hypocotyl development and provides a valuable resource for future investigations of seedling development.展开更多
Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (design...Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.展开更多
Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A...Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut.展开更多
基金supported by the Guangdong Provincial Key Research and Development Program-Modern Seed Industry,China(2022B0202060004)the Knowledge Innovation Program of Wuhan-Basic Research,China(2022020801010291)+1 种基金the Project of the Development for High-quality Seed Industry of Hubei Province,China(HBZY2023B003)the Innovation Program of the Chinese Academy of Agricultural Sciences(2023-2060299-089-031)。
文摘With the progress of urbanization,rural tourism has emerged as a popular leisure activity in China.The crop field with pattern art has been gaining popularity over the years,using strains with colorful leaves to create various impressive designs(Xu 2024).A lot of successful cases in rice fields have not only attracted tourists but also increased the income of farmers(Song et al.2020).Plants with colorful leaves also have gained significant popularity in ornamental agriculture.Therefore,developing plants with colorful leaves has gained significant popularity in ornamental agriculture.
基金supported by the Guangdong Provincial Key Research and Development Program-Modern Seed Industry(2022B0202060004)National Key Research and Development Project(2023YFD1202800)+9 种基金National Natural Science Foundation of China(32272121,32172051,32301869,32000471)Guangdong Science and Technology Plan Project(2023B1212060038)Guangdong Basic and Applied Basic Research Foundation(2021A1515010811,2023A1515010098,2023A1515010569)China Agriculture Research System of MOF and MARA(CARS-13)Guangzhou Basic and Applied Basic Research Foundation(2023A04J0776)Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science(R2020PY-JX004,R2020PY-JG005,R2021PY-QY003,R2022YJYB3025)the Foundation of the Director of Crop Research Institute of Guangdong Academy of Agriculture Sciences(202201,202306)Special Funds for the Revitalization of Agriculture through Seed Industry under the Provincial Rural Revitalization Strategy(2022-NPY-00-022)the Project of Collaborative Innovation Center of GDAAS(XTXM202203)Science and Technology Planning Project of Heyuan City(Heyuan She Nong Da Zhuan Xiang 2022002).
文摘Peanut seedlings develop from seeds by hypocotyl elongation and differentiation.However,the intricate gene regulatory networks and molecular crosstalk underlying hypocotyl growth in peanuts remain largely unknown.In this study,a single-nucleus gene expression landscape in peanut seedlings was initially developed from diverse tissues,including stems,roots,leaves,and hypocotyls.Core transcription factor interaction networks driving developmental trajectories were identified to decipher hypocotyl cell heterogeneity.Jasmonic acid and cytokinin regulate peanut hypocotyl expansion and differentiation based on the number and size of cortex cells and hormone levels between the hypocotyl at 3 and 7 d after germination.We further demonstrated that AhBHLH143 potentially represses hypocotyl elongation by promoting the JA pathway and inhibiting the cytokinin pathway.The single-nucleus transcriptomic atlas of peanut seedlings reveals new insights into hypocotyl development and provides a valuable resource for future investigations of seedling development.
文摘Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.
基金National Natural Science Foundation of China (31501246,31771841,31801401)the Natural Science Foundation of Guangdong Province (2017A030311007)+4 种基金the Modem Agroindustry Technology Research System (CARS-14)the Science and Technology Planning Project of Guangdong Province (2015B020231006, 2015A020209051, 2016B020201003, 2016LM3161, 2016LM3164, 2014A020208060 and S2013020012647)the International Science & Technology Cooperation Program of Guangdong Province (2013B050800021)the Agricultural Science and Technology Program of Guangdong (2013B020301014)the teamwork projects funded Guangdong Natural Science Foundation of Guangdong Province (no. 2017A030312004).
文摘Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut.
