A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegr...A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.展开更多
Mold manufacturing Extended Enterprise （EE） has the following characteristics： distributed in locality, tight cooperation and frequent information exchange. It needs a collaborative, highly efficient, reliable and ...Mold manufacturing Extended Enterprise （EE） has the following characteristics： distributed in locality, tight cooperation and frequent information exchange. It needs a collaborative, highly efficient, reliable and intelligent manufacturing management system. The background of the Collaborative Manufacturing is introduced. A mold Collaborative Manufacturing Execution System （c-MES） is proposed. The feature of Web Service platform is analyzed. The necessity and feasibility of importing the Web Service to mold c-MES are discussed. Based on Web Service, the model of mold c-MES is built. Every module＇ s function is described in detail, including the functions it supplies and the mechanism of information interaction among them. The feasibility of mold c-MES model is validated by a real mold manufacturing case.展开更多
文摘A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
文摘Mold manufacturing Extended Enterprise （EE） has the following characteristics： distributed in locality, tight cooperation and frequent information exchange. It needs a collaborative, highly efficient, reliable and intelligent manufacturing management system. The background of the Collaborative Manufacturing is introduced. A mold Collaborative Manufacturing Execution System （c-MES） is proposed. The feature of Web Service platform is analyzed. The necessity and feasibility of importing the Web Service to mold c-MES are discussed. Based on Web Service, the model of mold c-MES is built. Every module＇ s function is described in detail, including the functions it supplies and the mechanism of information interaction among them. The feasibility of mold c-MES model is validated by a real mold manufacturing case.
